##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049471_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7657980
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93994774078804	33.0	31.0	34.0	30.0	34.0
2	32.04082865194216	33.0	31.0	34.0	30.0	34.0
3	32.06516627100097	34.0	31.0	34.0	30.0	34.0
4	35.78126451100682	37.0	35.0	37.0	33.0	37.0
5	35.8440702378434	37.0	35.0	37.0	35.0	37.0
6	35.91694924771284	37.0	35.0	37.0	35.0	37.0
7	35.889437815194086	37.0	35.0	37.0	35.0	37.0
8	35.89113199564376	37.0	35.0	37.0	35.0	37.0
9	37.59157532404107	39.0	37.0	39.0	35.0	39.0
10-11	37.57555367081136	39.0	37.0	39.0	35.0	39.0
12-13	37.559152348269386	39.0	37.0	39.0	35.0	39.0
14-15	38.844119467535826	40.0	38.0	41.0	35.0	41.0
16-17	38.84554294996853	40.0	38.0	41.0	35.0	41.0
18-19	38.92384493038634	40.0	38.0	41.0	35.0	41.0
20-21	38.94946617776489	40.0	38.0	41.0	35.0	41.0
22-23	38.92158695896307	40.0	38.0	41.0	35.0	41.0
24-25	38.90137072700634	40.0	38.0	41.0	35.0	41.0
26-27	38.79659498980149	40.0	38.0	41.0	35.0	41.0
28-29	38.71444454020512	40.0	38.0	41.0	35.0	41.0
30-31	38.602684715812785	40.0	38.0	41.0	34.5	41.0
32-33	38.4706479384903	40.0	38.0	41.0	34.0	41.0
34-35	38.358585488601435	40.0	38.0	41.0	34.0	41.0
36-37	38.23325903175511	40.0	38.0	41.0	34.0	41.0
38-39	38.136755384579224	40.0	38.0	41.0	34.0	41.0
40-41	38.04379908278685	40.0	38.0	41.0	33.0	41.0
42-43	37.89255573924194	40.0	37.0	41.0	33.0	41.0
44-45	37.701887899942285	40.0	37.0	41.0	33.0	41.0
46-47	37.433842867179074	40.0	37.0	41.0	33.0	41.0
48-49	37.16505801791073	40.0	37.0	41.0	32.0	41.0
50-51	36.98458797228512	40.0	36.5	41.0	31.5	41.0
52-53	36.85257143528711	40.0	36.0	41.0	31.0	41.0
54-55	36.693773684444196	39.5	36.0	41.0	31.0	41.0
56-57	36.522592315467	39.0	35.0	41.0	31.0	41.0
58-59	36.32422140042152	39.0	35.0	41.0	31.0	41.0
60-61	36.119102426488446	39.0	35.0	41.0	31.0	41.0
62-63	35.88674180397442	38.5	35.0	40.5	30.0	41.0
64-65	35.65473799879342	38.0	35.0	40.0	30.0	41.0
66-67	35.37795952718602	37.0	35.0	40.0	30.0	41.0
68-69	35.078909190151975	37.0	35.0	40.0	29.0	41.0
70-71	34.792990122721655	36.0	34.0	39.0	29.0	41.0
72-73	34.458624206383405	36.0	34.0	39.0	29.0	41.0
74-75	34.13689726272464	35.0	34.0	39.0	29.0	40.0
76-77	33.82799635151828	35.0	34.0	37.5	29.0	39.5
78-79	33.53553907166119	35.0	34.0	37.0	29.0	39.0
80-81	33.244412103452866	35.0	34.0	36.5	29.0	39.0
82-83	32.993059723321295	35.0	34.0	36.0	28.0	38.0
84-85	32.732938908171604	35.0	34.0	36.0	28.0	37.0
86-87	32.5106900905983	35.0	33.0	35.5	27.0	37.0
88-89	32.31117278969128	35.0	33.0	35.0	27.0	36.5
90-91	32.13632864018972	35.0	33.0	35.0	27.0	36.0
92-93	31.987826554783375	35.0	33.0	35.0	27.0	36.0
94-95	31.838321071614185	35.0	33.0	35.0	26.0	36.0
96-97	31.70232829022797	35.0	33.0	35.0	26.0	35.5
98-99	31.56982272087417	35.0	33.0	35.0	25.5	35.0
100	31.461933564725946	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2970247274386004
1101	2	0.3630583016693052
1101	3	0.45309189436758857
1101	4	0.28418868337141134
1101	5	0.2645593731092646
1101	6	0.23310730574657867
1101	7	0.25529275203246016
1101	8	0.2657504395262009
1101	9	0.3393503593045324
1101	10-11	0.3203888442126157
1101	12-13	0.3204086534713042
1101	14-15	0.4224834204577732
1101	16-17	0.3985880208020163
1101	18-19	0.42681992481863773
1101	20-21	0.4160006607646096
1101	22-23	0.4096606580864517
1101	24-25	0.42438512012310525
1101	26-27	0.38599541041902086
1101	28-29	0.44510562875049686
1101	30-31	0.457513938399984
1101	32-33	0.46243408348748716
1101	34-35	0.39978794000352025
1101	36-37	0.38758380513657187
1101	38-39	0.37125714469843984
1101	40-41	0.4253657779698585
1101	42-43	0.41740843041807807
1101	44-45	0.37561135193634243
1101	46-47	0.3882401655661525
1101	48-49	0.3756272397865885
1101	50-51	0.3442901881230398
1101	52-53	0.3976891743822648
1101	54-55	0.3380479439547912
1101	56-57	0.3810742351609733
1101	58-59	0.38519921659624146
1101	60-61	0.3113665055956858
1101	62-63	0.3515117573908384
1101	64-65	0.2857971001954809
1101	66-67	0.3270031735665242
1101	68-69	0.32753944370170984
1101	70-71	0.32368502169110513
1101	72-73	0.37761556562118415
1101	74-75	0.38240258064841726
1101	76-77	0.3664363364200369
1101	78-79	0.35531538687668984
1101	80-81	0.3386633412001956
1101	82-83	0.3103792810058934
1101	84-85	0.3219267154760743
1101	86-87	0.30952872453812574
1101	88-89	0.3215288895966637
1101	90-91	0.3383956724156292
1101	92-93	0.3420304083838843
1101	94-95	0.3022087810106875
1101	96-97	0.3189364598392572
1101	98-99	0.3582265069084265
1101	100	0.32713015083421837
1102	1	0.2134612251808896
1102	2	0.24686030919591673
1102	3	0.30209577398586873
1102	4	0.20074972824638593
1102	5	0.2069989637273224
1102	6	0.17203153343176325
1102	7	0.1756269505680237
1102	8	0.20929319746480246
1102	9	0.2236368593914193
1102	10-11	0.2565664551664497
1102	12-13	0.2648197359364488
1102	14-15	0.3036668829431406
1102	16-17	0.27403037191475477
1102	18-19	0.2984927042938281
1102	20-21	0.3331162775115928
1102	22-23	0.27550666411926983
1102	24-25	0.26386100042729765
1102	26-27	0.24729172042193426
1102	28-29	0.2855696206458518
1102	30-31	0.3064981935123541
1102	32-33	0.300837329865395
1102	34-35	0.2926309832627112
1102	36-37	0.3152052272893684
1102	38-39	0.2782100065777797
1102	40-41	0.3168650070878556
1102	42-43	0.2898151415125554
1102	44-45	0.3428437234792483
1102	46-47	0.3452222429617535
1102	48-49	0.25318960261618173
1102	50-51	0.22509513421110228
1102	52-53	0.22980985882986715
1102	54-55	0.24718226744357708
1102	56-57	0.22692254403234102
1102	58-59	0.24167563684731164
1102	60-61	0.22601280591372586
1102	62-63	0.18555805697332062
1102	64-65	0.14867064007094655
1102	66-67	0.15208097817180288
1102	68-69	0.17163122999644997
1102	70-71	0.16614722694232853
1102	72-73	0.14041390408351617
1102	74-75	0.16844330660305218
1102	76-77	0.13876040430486114
1102	78-79	0.17493687009626768
1102	80-81	0.2288452478233296
1102	82-83	0.20737315149403912
1102	84-85	0.13152206831644975
1102	86-87	0.1436088138994478
1102	88-89	0.15750947204356436
1102	90-91	0.1676396858782141
1102	92-93	0.17683807941457275
1102	94-95	0.12147921639466475
1102	96-97	0.13798997212203545
1102	98-99	0.16606220529641647
1102	100	0.1870331304950703
1103	1	0.2609377543992828
1103	2	0.2724610770800808
1103	3	0.349728260539365
1103	4	0.21428343515076875
1103	5	0.23489212674729032
1103	6	0.23078483956324192
1103	7	0.22382328006771957
1103	8	0.20888235946244293
1103	9	0.2770381544633196
1103	10-11	0.2670662656666707
1103	12-13	0.2444651213051543
1103	14-15	0.3456135514211027
1103	16-17	0.3594206623673628
1103	18-19	0.3331556002586993
1103	20-21	0.3424420750909789
1103	22-23	0.3518008147614964
1103	24-25	0.32798513911271243
1103	26-27	0.3345297704177952
1103	28-29	0.34800752930600964
1103	30-31	0.36213539190006827
1103	32-33	0.418593688004016
1103	34-35	0.4042144835580217
1103	36-37	0.37184707704194864
1103	38-39	0.36284598384187916
1103	40-41	0.38642386023766306
1103	42-43	0.43412481010554416
1103	44-45	0.43527645579563057
1103	46-47	0.37463966454150466
1103	48-49	0.31423980992507694
1103	50-51	0.30989220360191894
1103	52-53	0.3233105913509462
1103	54-55	0.32241551182124084
1103	56-57	0.3284228581521873
1103	58-59	0.313557908022041
1103	60-61	0.28539152237291887
1103	62-63	0.3415128269587058
1103	64-65	0.3161584527091321
1103	66-67	0.29690450864274
1103	68-69	0.29244421521831043
1103	70-71	0.26437206341994823
1103	72-73	0.28169186778499267
1103	74-75	0.3062697791136628
1103	76-77	0.2537928124486797
1103	78-79	0.2639522231869549
1103	80-81	0.2761319993356395
1103	82-83	0.2730127826886104
1103	84-85	0.3139664126428414
1103	86-87	0.25071546845086345
1103	88-89	0.22349038691162093
1103	90-91	0.26062177255400343
1103	92-93	0.23112635847954976
1103	94-95	0.22349106419502718
1103	96-97	0.26365497091848056
1103	98-99	0.33238194624559014
1103	100	0.2972452404896515
1104	1	0.2301971681747439
1104	2	0.2054267644633896
1104	3	0.273976663508094
1104	4	0.16420507657981886
1104	5	0.15680004550199556
1104	6	0.14979930298701305
1104	7	0.1863704676160296
1104	8	0.1525518956042049
1104	9	0.2249808940257978
1104	10-11	0.22569713508641343
1104	12-13	0.17104725511806862
1104	14-15	0.3027263282383714
1104	16-17	0.26818350605382335
1104	18-19	0.2474711858371208
1104	20-21	0.2510698686970869
1104	22-23	0.25401428800271475
1104	24-25	0.24419649084340733
1104	26-27	0.2745283911718843
1104	28-29	0.25069363886670715
1104	30-31	0.25091184535838096
1104	32-33	0.32138855279354317
1104	34-35	0.3160702753886824
1104	36-37	0.32594664742806145
1104	38-39	0.3073124522567383
1104	40-41	0.3047462677585955
1104	42-43	0.2919053566687495
1104	44-45	0.30279072088245584
1104	46-47	0.30991765380702674
1104	48-49	0.259731210439341
1104	50-51	0.2931925875612933
1104	52-53	0.31643567817611995
1104	54-55	0.30834508655505033
1104	56-57	0.27711620446816454
1104	58-59	0.30384225043365376
1104	60-61	0.21244103699827122
1104	62-63	0.24639844413813705
1104	64-65	0.2256568033067765
1104	66-67	0.2946965679698792
1104	68-69	0.3028804054190033
1104	70-71	0.27544844277733915
1104	72-73	0.2903732337960321
1104	74-75	0.28816752712023685
1104	76-77	0.23815937601245452
1104	78-79	0.2653314561158382
1104	80-81	0.24704683289170504
1104	82-83	0.2665951635623074
1104	84-85	0.24730386437735064
1104	86-87	0.295530181307754
1104	88-89	0.2751830216561757
1104	90-91	0.26135102826900436
1104	92-93	0.27089119142303986
1104	94-95	0.25130708629655985
1104	96-97	0.25730480323793614
1104	98-99	0.31501707998547346
1104	100	0.33155825545845374
1105	1	0.1715324287585993
1105	2	0.16622069245629234
1105	3	0.2674857080065749
1105	4	0.1544507484627431
1105	5	0.16993451752715316
1105	6	0.11314317830060361
1105	7	0.09936773733518578
1105	8	0.09674632176677989
1105	9	0.1414070617741814
1105	10-11	0.14174925844768183
1105	12-13	0.15227410702592437
1105	14-15	0.2127280726748424
1105	16-17	0.20167938093292292
1105	18-19	0.22580105935755768
1105	20-21	0.20623274158289462
1105	22-23	0.18056693031507365
1105	24-25	0.232304852634023
1105	26-27	0.2155440678242755
1105	28-29	0.2739332826412664
1105	30-31	0.26559342229891314
1105	32-33	0.26795022763274545
1105	34-35	0.24326221070288057
1105	36-37	0.2289728818004022
1105	38-39	0.2350460388489921
1105	40-41	0.21043828637359496
1105	42-43	0.23207825610153066
1105	44-45	0.20745709678189428
1105	46-47	0.1812902583457756
1105	48-49	0.18183350416055788
1105	50-51	0.16482384589398436
1105	52-53	0.12843081878177998
1105	54-55	0.21758741297423967
1105	56-57	0.17391233540651285
1105	58-59	0.1751165708688589
1105	60-61	0.13418045289486713
1105	62-63	0.151825685494984
1105	64-65	0.14094874947468838
1105	66-67	0.11406553163362787
1105	68-69	0.16424137120444016
1105	70-71	0.19247667470045826
1105	72-73	0.14441920015585907
1105	74-75	0.14123922863156224
1105	76-77	0.1251468018040356
1105	78-79	0.09808634593977672
1105	80-81	0.1213519969826038
1105	82-83	0.06653721173093885
1105	84-85	0.08625250478058177
1105	86-87	0.10183399573375596
1105	88-89	0.0821633287120278
1105	90-91	0.08267284726491653
1105	92-93	0.08584970495294186
1105	94-95	0.06343954600454182
1105	96-97	0.1047601701498202
1105	98-99	0.17057439073017733
1105	100	0.14940290617446905
1106	1	0.1511096848285156
1106	2	0.17648237980406378
1106	3	0.2980765070929223
1106	4	0.15283454660563933
1106	5	0.1461121880128573
1106	6	0.1427250836541205
1106	7	0.15431139178394204
1106	8	0.1293511342610003
1106	9	0.16110947788394725
1106	10-11	0.15854088602618788
1106	12-13	0.15331264367939212
1106	14-15	0.20829815315375555
1106	16-17	0.2416490853508222
1106	18-19	0.2226246967647043
1106	20-21	0.2098074248606565
1106	22-23	0.18847708065074187
1106	24-25	0.16106753611485658
1106	26-27	0.17530124526998492
1106	28-29	0.19216651462781442
1106	30-31	0.23314794050310184
1106	32-33	0.19402971766201915
1106	34-35	0.1809114223603956
1106	36-37	0.19171256295668115
1106	38-39	0.22211928484109222
1106	40-41	0.2569441982209497
1106	42-43	0.2643402475720933
1106	44-45	0.35499620422321243
1106	46-47	0.31125813262850244
1106	48-49	0.3261354115208732
1106	50-51	0.3708052765276051
1106	52-53	0.2992313636749131
1106	54-55	0.3143407825976823
1106	56-57	0.37264237466140315
1106	58-59	0.31819544106369335
1106	60-61	0.3072014448830842
1106	62-63	0.30394749714551494
1106	64-65	0.3085264874279403
1106	66-67	0.29440985646172635
1106	68-69	0.3152293330592073
1106	70-71	0.39148636597978737
1106	72-73	0.400283108084885
1106	74-75	0.3691737108915234
1106	76-77	0.32731150032785195
1106	78-79	0.31146853012747044
1106	80-81	0.26002026646817455
1106	82-83	0.29195168206972966
1106	84-85	0.30446324435254013
1106	86-87	0.332193754599416
1106	88-89	0.2931411754454274
1106	90-91	0.2919110311546902
1106	92-93	0.2807497665290768
1106	94-95	0.2783050439998682
1106	96-97	0.2870109921872839
1106	98-99	0.3197312920829454
1106	100	0.2717693108730366
1107	1	0.10837023832019455
1107	2	0.13886081301312458
1107	3	0.23129525777486037
1107	4	0.09895502730652339
1107	5	0.12852921507403892
1107	6	0.1351569496388123
1107	7	0.05465147280870042
1107	8	0.08251397532738736
1107	9	0.08571651211241971
1107	10-11	0.10011372855826295
1107	12-13	0.12143892688236235
1107	14-15	0.18005447222351023
1107	16-17	0.17864289037438397
1107	18-19	0.19488090755100274
1107	20-21	0.1712516494182168
1107	22-23	0.18014373997117872
1107	24-25	0.1610405599797602
1107	26-27	0.17053781348617036
1107	28-29	0.16540459564183863
1107	30-31	0.15964331578750546
1107	32-33	0.11193127423256755
1107	34-35	0.08824869602667462
1107	36-37	0.10807052937939687
1107	38-39	0.10822112436373743
1107	40-41	0.13058670164772934
1107	42-43	0.07554931462189529
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2216	64-65	-0.6660926942149104
2216	66-67	-0.626332712135067
2216	68-69	-0.6663758518818668
2216	70-71	-0.6641924478616872
2216	72-73	-0.6470349922643379
2216	74-75	-0.6457975506528442
2216	76-77	-0.5674668531042499
2216	78-79	-0.6067672353064566
2216	80-81	-0.5379380595546621
2216	82-83	-0.6276449720085182
2216	84-85	-0.7263526033151706
2216	86-87	-0.6192040806148782
2216	88-89	-0.623361009783423
2216	90-91	-0.5576134357113531
2216	92-93	-0.5864289476448512
2216	94-95	-0.6415535855924155
2216	96-97	-0.670258800065767
2216	98-99	-0.7716359747100334
2216	100	-0.760447797105158
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	499.0
3	76.0
4	84.0
5	211.0
6	622.0
7	1634.0
8	3329.0
9	5202.0
10	6300.0
11	8137.0
12	11089.0
13	15424.0
14	18530.0
15	24946.0
16	33174.0
17	48353.0
18	49060.0
19	26470.0
20	19110.0
21	18349.0
22	19455.0
23	21129.0
24	24421.0
25	29844.0
26	38544.0
27	50214.0
28	65741.0
29	86598.0
30	113010.0
31	147082.0
32	190744.0
33	251110.0
34	346380.0
35	521861.0
36	891186.0
37	1653939.0
38	2150340.0
39	764124.0
40	1659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99015909270678	19.993985894307322	14.959198868825164	23.05665614416073
2	15.82333722469894	20.425895601712202	39.470643694551306	24.280123479037552
3	18.253116357054992	25.36223651667933	30.645117903154617	25.739529223111056
4	13.205675648147421	17.58907701508753	37.020402247067764	32.18484508969728
5	13.347735786295182	37.415582466776605	34.86690464408584	14.369777102842368
6	30.78323528947043	37.406272448027	17.232563159386817	14.57792910311575
7	26.84512103714034	32.982444456632166	21.963220065865933	18.209214440361556
8	25.42170389580542	35.69727264892308	19.79414937098295	19.086874084288546
9	25.453761958114278	16.641007158545726	21.097874374182226	36.80735650915777
10-11	24.349384563553315	26.800337686961832	27.98051183210194	20.869765917382914
12-13	25.14423516384216	24.45195730466781	28.330708620288902	22.073098911201125
14-15	22.350593759712094	26.157877142536286	26.321347143763763	25.170181953987864
16-17	21.39268449382213	29.34802650307261	27.098810652417477	22.16047835068778
18-19	21.354906907565702	27.59502505882752	30.001612696820835	21.04845533678594
20-21	22.150762994941225	26.71551766915035	30.08303103429364	21.050688301614787
22-23	22.09215746188943	26.46391084855275	30.08448703182824	21.359444657729583
24-25	21.805965802992432	26.62460596658649	29.9899777748179	21.579450455603176
26-27	21.611730508567533	26.768553848403887	30.035165931485846	21.58454971154273
28-29	21.645988890020607	26.696720283939108	30.22447172753128	21.432819098509007
30-31	21.812344769769574	26.771080624394422	29.948765862538163	21.46780874329784
32-33	21.6703033959347	26.767770351972715	30.039808147840553	21.522118104252037
34-35	21.91394466948203	26.574762535289985	29.930843381675064	21.58044941355292
36-37	21.9469429275083	26.63539210078898	29.7046479619952	21.71301700970752
38-39	21.772876137049195	26.789388324336183	29.788384143076897	21.649351395537728
40-41	21.865158958367612	26.587637993308938	29.80317916735223	21.744023880971223
42-43	21.84365851046882	26.819970801699668	29.55370084539265	21.782669842438867
44-45	21.915067681033378	26.848411722151273	29.40343275903045	21.8330878377849
46-47	22.125292831791153	26.84448118172155	29.011717189128206	22.018508797359097
48-49	22.135687217778056	27.002780106503284	28.658673697241312	22.20285897847735
50-51	22.149065419340346	27.131606507199024	28.473494315733394	22.245833757727233
52-53	22.246656428979968	27.228244262847383	28.347370977725195	22.177728330447454
54-55	22.196839114231167	27.406339530790103	28.26428118119922	22.132540173779507
56-57	22.182853702934715	27.18383960261061	28.34012363573684	22.293183058717833
58-59	22.173791260880808	26.903667807959803	28.531708100569602	22.39083283058979
60-61	22.149052361066495	27.091054037748858	28.46069067822063	22.29920292296402
62-63	21.990152755687532	27.128942619333035	28.533712545606022	22.347192079373414
64-65	22.05544412495201	27.06256741333877	28.489464584655483	22.39252387705374
66-67	22.003139209034238	27.03024165641592	28.53415652691702	22.43246260763282
68-69	22.10077592263234	27.0992611628654	28.394733336989653	22.405229577512607
70-71	22.095069456958623	26.992313900010185	28.41106923757962	22.501547405451568
72-73	22.0472696455201	27.09254268096809	28.358013470915306	22.502174202596507
74-75	22.035252381862108	27.18035006363951	28.175508185645327	22.60888936885306
76-77	22.132527115505656	27.049345911062712	28.06407825562355	22.75404871780809
78-79	22.087939639434943	27.126474605574842	28.167793595700168	22.617792159290047
80-81	21.978477583286114	27.13091421498311	28.17768000889009	22.712928192840685
82-83	21.93704560961357	27.14467691966166	28.272391019760573	22.6458864509642
84-85	21.937543296537125	27.02185769158697	28.10186675407045	22.938732257805455
86-87	21.988554752692256	27.082347292290216	28.081643643390453	22.84745431162708
88-89	21.89555364918059	26.996996512700587	28.120655035893176	22.986794802225642
90-91	21.82764688103766	27.001549440915674	28.086667358325005	23.084136319721658
92-93	21.840805206187962	27.073860624050987	28.015762414135143	23.06957175562591
94-95	21.723984495865903	26.880013371711716	28.176312025758826	23.21969010666356
96-97	21.81027688474965	27.0181374648918	27.983208524858565	23.18837712549998
98-99	21.657030420382007	27.051145406594923	28.005278737281746	23.286545435741324
100	21.73057782645906	26.880843418899047	28.053921311302442	23.334657443339452
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1405.0
1	1111.5
2	1277.5
3	1768.0
4	1251.5
5	931.0
6	2376.0
7	6526.0
8	11206.0
9	12969.5
10	10051.0
11	6220.5
12	5428.5
13	5860.0
14	6539.0
15	7220.0
16	7983.0
17	8816.0
18	9710.5
19	10713.5
20	11978.5
21	13831.0
22	16959.0
23	22204.5
24	29081.5
25	37623.5
26	48901.0
27	62145.5
28	78337.0
29	97435.0
30	114071.5
31	129363.5
32	144298.0
33	158250.0
34	172601.0
35	186412.5
36	200909.5
37	216169.0
38	231583.0
39	243178.5
40	252457.0
41	259654.0
42	264506.5
43	268973.5
44	270093.5
45	270838.5
46	270108.0
47	263144.0
48	257233.0
49	255179.5
50	250287.0
51	241242.0
52	226435.0
53	215819.0
54	210476.0
55	201431.5
56	186029.5
57	165809.0
58	150010.5
59	139208.5
60	125900.5
61	109384.0
62	94304.0
63	79318.0
64	64574.5
65	52774.5
66	42937.0
67	33930.0
68	26875.0
69	21123.5
70	15754.5
71	11703.0
72	8767.5
73	6216.0
74	4255.5
75	2848.5
76	1732.5
77	1084.5
78	695.0
79	382.5
80	199.0
81	102.0
82	62.0
83	34.5
84	21.5
85	19.0
86	13.0
87	9.0
88	5.0
89	3.5
90	3.5
91	1.0
92	1.5
93	1.5
94	0.5
95	1.0
96	1.0
97	0.0
98	0.5
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12137665546266771
2	0.0
3	0.0
4	0.0
5	0.012418418434104031
6	1.6975756008764714E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.3058273852895934E-5
76-77	0.0
78-79	0.0
80-81	1.4364101238185527E-4
82-83	7.182050619092764E-4
84-85	4.570395848513577E-4
86-87	5.419183648951812E-4
88-89	6.333262818654528E-4
90-91	7.377924726886203E-4
92-93	2.0893238164633495E-4
94-95	2.938111616901585E-4
96-97	6.072097341596608E-4
98-99	0.0011491280990548421
100	7.83496431173756E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7657980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.97374763068176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.79626545403606	30.04078385201844
2	16.79558347863757	15.107206653565886
3	6.477427495832262	8.739425684807973
4	3.1470692995281895	5.661420018129889
5	1.785959063200306	4.016063609354969
6	1.0919731697086295	2.9466075452370917
7	0.7534182460777838	2.3718829441617197
8	0.5458285201793932	1.9638363292941212
9	0.3901827531950234	1.579318260483381
>10	2.0186179504839186	16.3517242176094
>50	0.13299489585975238	4.104200559173121
>100	0.06142190661689572	4.793476630164282
>500	0.002297996432986908	0.6855176355201307
>1k	8.434344323179882E-4	0.6111826336928855
>5k	2.908394468225261E-5	0.10492888213696075
>10k+	8.725183404675783E-5	0.9224245446497893
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28071	0.36655880532464175	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26852	0.3506407694979616	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15598	0.20368295555747076	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8022	0.10475347284793117	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.6116547705791865E-5	0.0	0.0	0.0
4	0.0	2.6116547705791865E-5	0.0	0.0	0.0
5	0.0	3.91748215586878E-5	0.0	0.0	0.0
6	0.0	3.91748215586878E-5	0.0	0.0	0.0
7	0.0	3.91748215586878E-5	0.0	0.0	0.0
8	0.0	3.91748215586878E-5	0.0	0.0	0.0
9	9.140791697027154E-5	3.91748215586878E-5	0.0	1.3058273852895933E-5	0.0
10-11	1.4364101238185527E-4	3.91748215586878E-5	0.0	1.9587410779343897E-5	0.0
12-13	1.6975756008764714E-4	5.223309541158373E-5	0.0	3.91748215586878E-5	0.0
14-15	1.6975756008764714E-4	7.83496431173756E-5	0.0	5.223309541158373E-5	0.0
16-17	1.8281583394054308E-4	7.83496431173756E-5	0.0	5.223309541158373E-5	0.0
18-19	1.8934497086699106E-4	1.0446619082316746E-4	0.0	6.529136926447967E-5	0.0
20-21	1.95874107793439E-4	1.3058273852895933E-4	0.0	9.79370538967195E-5	0.0
22-23	1.95874107793439E-4	1.566992862347512E-4	0.0	2.6116547705791867E-4	0.0
24-25	1.95874107793439E-4	1.566992862347512E-4	0.0	4.243939002191178E-4	0.0
26-27	1.95874107793439E-4	1.566992862347512E-4	0.0	8.879626219969234E-4	0.0
28-29	1.95874107793439E-4	1.566992862347512E-4	0.0	0.002742237509108146	0.0
30-31	1.95874107793439E-4	1.566992862347512E-4	0.0	0.00908855860161557	0.0
32-33	1.95874107793439E-4	1.566992862347512E-4	0.0	0.023269844005860553	0.0
34-35	2.2199065549923086E-4	1.566992862347512E-4	0.0	0.04677473694107323	0.0
36-37	2.2199065549923086E-4	1.6975756008764714E-4	0.0	0.08372965194476872	0.0
38-39	2.2199065549923086E-4	1.6975756008764714E-4	0.0	0.12984494605627073	0.0
40-41	2.2199065549923086E-4	1.6975756008764714E-4	0.0	0.17599288585240494	0.0
42-43	2.2199065549923086E-4	1.8281583394054308E-4	0.0	0.22483735919916217	0.0
44-45	2.2199065549923086E-4	1.8281583394054308E-4	0.0	0.289665159741864	0.0
46-47	2.2199065549923086E-4	1.8281583394054308E-4	0.0	0.3716711195380505	0.0
48-49	2.2851979242567884E-4	1.8281583394054308E-4	0.0	0.4569677643451667	0.0
50-51	2.350489293521268E-4	3.6563166788108615E-4	0.0	0.5409194069454347	0.0
52-53	2.4810720320502276E-4	3.91748215586878E-4	0.0	0.6264706880926825	0.0
54-55	2.4810720320502276E-4	4.1786476329266984E-4	0.0	0.7259486183040436	0.0
56-57	2.546363401314707E-4	4.505104479249097E-4	0.0	0.8489183831767646	0.0
58-59	2.6116547705791867E-4	4.570395848513577E-4	0.0	0.9872381489635648	0.0
60-61	2.6116547705791867E-4	4.570395848513577E-4	0.0	1.1285417303257517	0.0
62-63	2.8728202476371054E-4	4.8315613255714955E-4	0.0	1.2744157075364522	0.0
64-65	2.8728202476371054E-4	4.962144064100455E-4	0.0	1.4287253296561233	0.0
66-67	2.8728202476371054E-4	5.158018171893894E-4	0.0	1.612305072617061	0.0
68-69	2.8728202476371054E-4	5.223309541158373E-4	0.0	1.8226542769764351	0.0
70-71	2.938111616901585E-4	5.223309541158373E-4	0.0	2.0493002071042232	0.0
72-73	3.0034029861660645E-4	5.419183648951813E-4	0.0	2.281378379154816	0.0
74-75	3.133985724695024E-4	5.484475018216293E-4	0.0	2.519926925899519	0.0
76-77	3.133985724695024E-4	5.484475018216293E-4	0.0	2.785284108864217	0.0
78-79	3.133985724695024E-4	5.484475018216293E-4	0.0	3.0862707920365424	0.0
80-81	3.133985724695024E-4	5.484475018216293E-4	0.0	3.413217323628424	0.0
82-83	3.133985724695024E-4	5.484475018216293E-4	0.0	3.7520338261525885	0.0
84-85	3.6563166788108615E-4	5.81093186453869E-4	0.0	4.090693629390518	0.0
86-87	3.6563166788108615E-4	5.87622323380317E-4	0.0	4.4558551994129	0.0
88	3.6563166788108615E-4	5.87622323380317E-4	0.0	4.750848134886746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15520	0.0	57.645878	1
GTATCAA	27905	0.0	40.949116	1
GTGGTAT	6140	0.0	34.722237	1
ATCAACG	34655	0.0	32.66184	3
TCAACGC	35030	0.0	32.32561	4
TATCAAC	35660	0.0	31.820427	2
CAACGCA	36735	0.0	30.799671	5
TGGTATC	6825	0.0	30.166264	2
AACGCAG	38075	0.0	29.748484	6
ACGCAGA	42860	0.0	26.240868	7
CGCAGAG	43585	0.0	25.631842	8
GCAGAGT	46610	0.0	23.675907	9
GTACATG	25830	0.0	23.267214	1
TACATGG	26390	0.0	22.247084	2
GAGTACT	31365	0.0	21.974775	12-13
CAGAGTA	45300	0.0	21.377752	10-11
ACATGGG	26670	0.0	20.938398	3
AGAGTAC	43695	0.0	20.436642	10-11
GTACTTT	34030	0.0	20.088127	14-15
AGTACTT	32330	0.0	19.63981	12-13
>>END_MODULE