FastQCFastQC Report
Wed 25 May 2016
SRR2049470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7465473
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77100.043.8553921
GTATCAA130800.035.054631
TCAACGC136100.032.3585054
ATCAACG136300.032.347243
CAACGCA149300.029.529485
TATCAAC158150.028.2946172
AACGCAG157850.027.8408646
ACGCAGA175950.024.5502827
CGCAGAG176050.024.293288
GTACATG201400.022.7663651
TACATGG201700.021.416362
ACATGGG206600.020.2028623
GCAGAGT206550.019.8191649
GTGGTAT43300.019.7666021
AGAGTAC179300.017.43204510-11
CAGAGTA198600.017.38279210-11
CATGGGG139000.016.365814
TGGTATC48200.015.8955892
GTATAGG26650.015.7051231
GAGTACA157800.014.7220561
Produced by FastQC (version 0.11.5)