FastQCFastQC Report
Wed 25 May 2016
SRR2049470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7465473
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195710.26215351659566644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192150.2573848971123464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101140.1354770153210654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132400.057.4840971
GTATCAA236750.039.1454661
ATCAACG285400.031.9187743
TCAACGC287450.031.7238464
TATCAAC302700.030.3895872
CAACGCA301300.030.2811835
AACGCAG313500.029.1588446
GTGGTAT57000.028.2407971
ACGCAGA351000.025.7623947
CGCAGAG353800.025.39918
TGGTATC61000.025.0437852
GCAGAGT389250.022.9048239
GTACATG195300.021.6662221
CAGAGTA377850.021.28231210-11
GAGTACT282250.021.15577512-13
AGAGTAC361400.020.3133310-11
TACATGG198050.020.1264612
ACATGGG198800.019.459423
GTACTTT303400.019.45639214-15
AGTACTT293650.018.62992912-13
Produced by FastQC (version 0.11.5)