##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7465473 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95465685831293 33.0 31.0 34.0 30.0 34.0 2 32.1826533965095 33.0 31.0 34.0 30.0 34.0 3 32.185598019040455 34.0 31.0 34.0 30.0 34.0 4 35.86220698942987 37.0 35.0 37.0 35.0 37.0 5 35.911444994844935 37.0 35.0 37.0 35.0 37.0 6 35.96927803502872 37.0 35.0 37.0 35.0 37.0 7 35.94232542264904 37.0 35.0 37.0 35.0 37.0 8 35.951033377255534 37.0 35.0 37.0 35.0 37.0 9 37.68656895551025 39.0 37.0 39.0 35.0 39.0 10-11 37.660265665685216 39.0 37.0 39.0 35.0 39.0 12-13 37.63954675075511 39.0 37.0 39.0 35.0 39.0 14-15 38.95239049153349 40.0 38.0 41.0 36.0 41.0 16-17 38.961571356563745 40.0 38.0 41.0 36.0 41.0 18-19 39.038427437886384 40.0 38.5 41.0 36.0 41.0 20-21 39.06397591954321 40.0 39.0 41.0 36.0 41.0 22-23 39.03890034831015 40.0 39.0 41.0 35.5 41.0 24-25 39.019986007584514 40.0 39.0 41.0 35.0 41.0 26-27 38.92679901193132 40.0 38.5 41.0 35.0 41.0 28-29 38.85123621771855 40.0 38.0 41.0 35.0 41.0 30-31 38.749603876405416 40.0 38.0 41.0 35.0 41.0 32-33 38.623079274414366 40.0 38.0 41.0 35.0 41.0 34-35 38.514365801068465 40.0 38.0 41.0 34.5 41.0 36-37 38.38766317954669 40.0 38.0 41.0 34.0 41.0 38-39 38.288004055469756 40.0 38.0 41.0 34.0 41.0 40-41 38.20070884992819 40.0 38.0 41.0 34.0 41.0 42-43 38.06518923851175 40.0 38.0 41.0 33.5 41.0 44-45 37.90057615907257 40.0 38.0 41.0 33.0 41.0 46-47 37.665021358994935 40.0 38.0 41.0 33.0 41.0 48-49 37.427058673978195 40.0 37.0 41.0 33.0 41.0 50-51 37.27318938799993 40.0 37.0 41.0 32.0 41.0 52-53 37.16244918439863 40.0 37.0 41.0 32.0 41.0 54-55 37.02357988569512 40.0 37.0 41.0 32.0 41.0 56-57 36.875914694219645 40.0 36.0 41.0 31.0 41.0 58-59 36.70344029105725 39.5 36.0 41.0 31.0 41.0 60-61 36.519683414567304 39.0 35.0 41.0 31.0 41.0 62-63 36.30533952771646 39.0 35.0 41.0 31.0 41.0 64-65 36.086843928040466 39.0 35.0 41.0 31.0 41.0 66-67 35.82658057968999 38.0 35.0 40.0 30.0 41.0 68-69 35.534946278688565 37.5 35.0 40.0 30.0 41.0 70-71 35.24983353365553 37.0 35.0 40.0 30.0 41.0 72-73 34.91524100348364 36.5 35.0 39.0 29.5 41.0 74-75 34.5871961495273 36.0 34.0 39.0 29.0 41.0 76-77 34.262245942085656 36.0 34.0 39.0 29.0 40.0 78-79 33.94498774558558 35.0 34.0 37.5 29.0 39.5 80-81 33.62796302391021 35.0 34.0 37.0 29.0 39.0 82-83 33.344208665679986 35.0 34.0 36.5 29.0 39.0 84-85 33.05455494916397 35.0 34.0 36.0 29.0 37.5 86-87 32.80373909328987 35.0 34.0 36.0 29.0 37.0 88-89 32.582033984986616 35.0 34.0 35.5 28.5 37.0 90-91 32.38548843455733 35.0 34.0 35.0 28.0 36.0 92-93 32.21879102636899 35.0 33.5 35.0 27.0 36.0 94-95 32.05785380243154 35.0 33.0 35.0 27.0 36.0 96-97 31.91480258518114 35.0 33.0 35.0 27.0 36.0 98-99 31.77978153561067 35.0 33.0 35.0 27.0 35.5 100 31.670194909284383 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28463428296673854 1101 2 0.3620208702751313 1101 3 0.4542225947274261 1101 4 0.2567311185434562 1101 5 0.23658872738153747 1101 6 0.23725202606382823 1101 7 0.24636765350122403 1101 8 0.2551951548152189 1101 9 0.32555923566830813 1101 10-11 0.28155989975005724 1101 12-13 0.289226389458868 1101 14-15 0.4219264696732026 1101 16-17 0.3995972419009348 1101 18-19 0.39332696346338025 1101 20-21 0.40351038802751305 1101 22-23 0.3908994721470691 1101 24-25 0.4102738360181064 1101 26-27 0.3667364997800462 1101 28-29 0.3932734137089824 1101 30-31 0.41633908861886937 1101 32-33 0.3882105124187447 1101 34-35 0.4011167673971272 1101 36-37 0.44992604982194706 1101 38-39 0.419566153465766 1101 40-41 0.44295170974585574 1101 42-43 0.417035604567225 1101 44-45 0.4370132281780741 1101 46-47 0.4313521665480238 1101 48-49 0.40519677576244106 1101 50-51 0.37037588472904304 1101 52-53 0.42077631704884766 1101 54-55 0.39504983499549695 1101 56-57 0.4166750452944896 1101 58-59 0.3946906630595137 1101 60-61 0.2955433523586066 1101 62-63 0.3487460549283341 1101 64-65 0.3051132459548569 1101 66-67 0.39064080535201384 1101 68-69 0.3323699077562381 1101 70-71 0.3103041121004608 1101 72-73 0.3411158661731548 1101 74-75 0.3204327966051679 1101 76-77 0.3400874988190381 1101 78-79 0.3523096438867839 1101 80-81 0.303899212770105 1101 82-83 0.2711353447291245 1101 84-85 0.32115449841843 1101 86-87 0.2778759541933198 1101 88-89 0.3197228777913992 1101 90-91 0.30512443303207704 1101 92-93 0.2925423039486219 1101 94-95 0.31069005987321674 1101 96-97 0.27112513015548956 1101 98-99 0.3330300177024661 1101 100 0.3555555427769299 1102 1 0.23801501633758804 1102 2 0.28878001221934824 1102 3 0.3574847823127101 1102 4 0.22854724412102456 1102 5 0.2115125231479169 1102 6 0.1947570614496712 1102 7 0.21944362314312116 1102 8 0.18003746590191838 1102 9 0.23665623242108325 1102 10-11 0.24107689626767126 1102 12-13 0.2516892260541752 1102 14-15 0.3169417438241524 1102 16-17 0.3621907772784141 1102 18-19 0.35513080282742493 1102 20-21 0.3410263991867879 1102 22-23 0.2600323716278723 1102 24-25 0.3036889791534847 1102 26-27 0.2755460089246853 1102 28-29 0.3276014894624524 1102 30-31 0.33266036100928886 1102 32-33 0.36427275935866277 1102 34-35 0.38481508967030464 1102 36-37 0.35410391579635814 1102 38-39 0.3081382680520477 1102 40-41 0.28152007659126355 1102 42-43 0.26405913780084944 1102 44-45 0.3162016992133161 1102 46-47 0.3696782522521076 1102 48-49 0.3839365962189447 1102 50-51 0.31964477470871344 1102 52-53 0.34232080401236686 1102 54-55 0.35875316772711585 1102 56-57 0.3963756368445672 1102 58-59 0.3768888989955457 1102 60-61 0.3283947786090664 1102 62-63 0.34015150324394483 1102 64-65 0.2939502124036295 1102 66-67 0.30900518035686986 1102 68-69 0.32580641636531027 1102 70-71 0.3000512180799362 1102 72-73 0.27126870373692924 1102 74-75 0.25754501432367505 1102 76-77 0.2812521656988949 1102 78-79 0.25183473678374213 1102 80-81 0.2783381477342459 1102 82-83 0.20030569237590612 1102 84-85 0.12348531807463559 1102 86-87 0.17014103536760672 1102 88-89 0.17572590067796057 1102 90-91 0.2187351678291165 1102 92-93 0.2034180002074919 1102 94-95 0.18249453709443486 1102 96-97 0.22141365746472985 1102 98-99 0.2884541379921366 1102 100 0.3335160646371058 1103 1 0.3183357189852636 1103 2 0.31193026740820784 1103 3 0.38070315081766637 1103 4 0.2204810697897912 1103 5 0.20150533216197175 1103 6 0.19503245597089602 1103 7 0.2147733233276483 1103 8 0.18374103071293746 1103 9 0.2610725632233084 1103 10-11 0.2656650003389345 1103 12-13 0.24595872409077657 1103 14-15 0.360075613890416 1103 16-17 0.3467648769035776 1103 18-19 0.34068251720476184 1103 20-21 0.34876999794764885 1103 22-23 0.3740540144018496 1103 24-25 0.3283527141610989 1103 26-27 0.3298674669722601 1103 28-29 0.3521739245684472 1103 30-31 0.39461338882808406 1103 32-33 0.4106770409539635 1103 34-35 0.3756258535015462 1103 36-37 0.3664854024964228 1103 38-39 0.38188270743185626 1103 40-41 0.35632934285837337 1103 42-43 0.3953457791850923 1103 44-45 0.39093785493026445 1103 46-47 0.39824864391992776 1103 48-49 0.41242967830547883 1103 50-51 0.36699247894552656 1103 52-53 0.37357623421104336 1103 54-55 0.33384543901750163 1103 56-57 0.3704320245694106 1103 58-59 0.33846541788185647 1103 60-61 0.2591627641817382 1103 62-63 0.3466871500770168 1103 64-65 0.32949757004428193 1103 66-67 0.35230753412860594 1103 68-69 0.3692979877681495 1103 70-71 0.34359627391621217 1103 72-73 0.3789154722593153 1103 74-75 0.3344654934590565 1103 76-77 0.35048793893634667 1103 78-79 0.3612440099240999 1103 80-81 0.37374145577354057 1103 82-83 0.3718019849990739 1103 84-85 0.3502907456919715 1103 86-87 0.327412413409121 1103 88-89 0.3090340804602789 1103 90-91 0.30596324282992526 1103 92-93 0.32943547672987705 1103 94-95 0.32324617198816696 1103 96-97 0.34464947903941834 1103 98-99 0.3894843557682073 1103 100 0.33732818504566 1104 1 0.22356893886160023 1104 2 0.201717566720383 1104 3 0.3053277808126751 1104 4 0.1781035159091573 1104 5 0.16939691402748736 1104 6 0.12189421493755503 1104 7 0.13563777539396682 1104 8 0.15298367477709007 1104 9 0.18738611093714752 1104 10-11 0.21959688265695831 1104 12-13 0.18127975761178305 1104 14-15 0.30954014062027824 1104 16-17 0.2751103884644408 1104 18-19 0.27464681807627755 1104 20-21 0.2807813060354931 1104 22-23 0.26216302636034783 1104 24-25 0.29179723225527 1104 26-27 0.270324304884511 1104 28-29 0.2734668071108146 1104 30-31 0.34057095624914524 1104 32-33 0.3156295573644101 1104 34-35 0.33681435803875104 1104 36-37 0.3288046106213187 1104 38-39 0.32512963324467137 1104 40-41 0.34034640891302104 1104 42-43 0.3471480219485201 1104 44-45 0.2954180998361551 1104 46-47 0.25987808068553164 1104 48-49 0.3064713890900066 1104 50-51 0.32238201317386483 1104 52-53 0.36943110233877974 1104 54-55 0.30046983429552654 1104 56-57 0.30631069087345963 1104 58-59 0.36055593953823717 1104 60-61 0.3350440869697522 1104 62-63 0.34932892500546586 1104 64-65 0.3552668347574297 1104 66-67 0.372701271411394 1104 68-69 0.37509675968045286 1104 70-71 0.30474164334760445 1104 72-73 0.3267197703374123 1104 74-75 0.2950146719643598 1104 76-77 0.3093560110111113 1104 78-79 0.2746686874383215 1104 80-81 0.2809203046910298 1104 82-83 0.2694842636488488 1104 84-85 0.2533759074296569 1104 86-87 0.2623171832392046 1104 88-89 0.2857991381694944 1104 90-91 0.3052928792692242 1104 92-93 0.3425137095364974 1104 94-95 0.31483649657678114 1104 96-97 0.26536102271534645 1104 98-99 0.33653873326029426 1104 100 0.3493834805140743 1105 1 0.17365727010210108 1105 2 0.18652542388213078 1105 3 0.26022089272020565 1105 4 0.15693535334939668 1105 5 0.17432130664179368 1105 6 0.16138299830926428 1105 7 0.11851582407641104 1105 8 0.1107400712736677 1105 9 0.1598943554090937 1105 10-11 0.1210667513679482 1105 12-13 0.15309446706211105 1105 14-15 0.2090557233015744 1105 16-17 0.2333686212288626 1105 18-19 0.1938897701643043 1105 20-21 0.1949142761820042 1105 22-23 0.1997349665184629 1105 24-25 0.23151101341188252 1105 26-27 0.18582962932504188 1105 28-29 0.266367710981001 1105 30-31 0.19452761967808385 1105 32-33 0.215722626952207 1105 34-35 0.18024305394278883 1105 36-37 0.23281805054520532 1105 38-39 0.22701793126937275 1105 40-41 0.1947356108893672 1105 42-43 0.15821930432061748 1105 44-45 0.13146044145611313 1105 46-47 0.14112210390656088 1105 48-49 0.12142611518436297 1105 50-51 0.1337051101718174 1105 52-53 0.13816872914512146 1105 54-55 0.10968573251453506 1105 56-57 0.1202038996729442 1105 58-59 0.1512250840466507 1105 60-61 0.12076929312592455 1105 62-63 0.10545734614378688 1105 64-65 0.12997996331216655 1105 66-67 0.06836605241682747 1105 68-69 0.1362550717195532 1105 70-71 0.113999138792785 1105 72-73 0.16745723284446257 1105 74-75 0.1302970407622368 1105 76-77 0.08438883523353269 1105 78-79 0.07234306869042939 1105 80-81 0.059891384817191806 1105 82-83 0.016052007719977723 1105 84-85 0.034088492882936805 1105 86-87 0.004138482812876987 1105 88-89 0.04652896690376451 1105 90-91 0.05127523402338596 1105 92-93 0.051801170390902485 1105 94-95 -0.04061932716459893 1105 96-97 0.0014953094344321016 1105 98-99 0.017836825791953004 1105 100 0.004702604507659913 1106 1 0.2241510515102938 1106 2 0.18895425545662192 1106 3 0.2687574484889197 1106 4 0.1212146396973992 1106 5 0.14828253048822404 1106 6 0.15366501103598296 1106 7 0.1458053059776745 1106 8 0.1496923813031259 1106 9 0.17939767275513674 1106 10-11 0.18213434398112582 1106 12-13 0.20276070503842192 1106 14-15 0.24739105310584364 1106 16-17 0.2201352004283592 1106 18-19 0.208200140924788 1106 20-21 0.23290192475230498 1106 22-23 0.24101036419955335 1106 24-25 0.2191056831920264 1106 26-27 0.21142241560703923 1106 28-29 0.24906333789570567 1106 30-31 0.23648627469071215 1106 32-33 0.21562430983167502 1106 34-35 0.21475990880982465 1106 36-37 0.21687723158157013 1106 38-39 0.1965763719455893 1106 40-41 0.21451377081922374 1106 42-43 0.22659484073742675 1106 44-45 0.2325524842392781 1106 46-47 0.20528211776019845 1106 48-49 0.20972145095823436 1106 50-51 0.23088852571418528 1106 52-53 0.18810906744916167 1106 54-55 0.22752089674479947 1106 56-57 0.2698978148942217 1106 58-59 0.19477046625819838 1106 60-61 0.1905321061689378 1106 62-63 0.17451870173600526 1106 64-65 0.2559465641804479 1106 66-67 0.2487726921969653 1106 68-69 0.2231883081936985 1106 70-71 0.20074402884527132 1106 72-73 0.25112956018791266 1106 74-75 0.2184772123231582 1106 76-77 0.23655526486036393 1106 78-79 0.20091271075749262 1106 80-81 0.11646792940778994 1106 82-83 0.14662780681400278 1106 84-85 0.13479855371799232 1106 86-87 0.17443567952031458 1106 88-89 0.14502126834663898 1106 90-91 0.20136067024385795 1106 92-93 0.19543139629047346 1106 94-95 0.10789174572263249 1106 96-97 0.15761627165993275 1106 98-99 0.1555297155631159 1106 100 0.10360478400311379 1107 1 0.06729935295919276 1107 2 0.09479700049735129 1107 3 0.17692882089475148 1107 4 0.08498869045991597 1107 5 0.08427734639472106 1107 6 0.07645810633287198 1107 7 0.07871107147212086 1107 8 0.07269970527827496 1107 9 0.034762162722522305 1107 10-11 0.07389440236402578 1107 12-13 0.07926377032382703 1107 14-15 0.14200497420188896 1107 16-17 0.1296793314215492 1107 18-19 0.12372830137330482 1107 20-21 0.09721840756751021 1107 22-23 0.10835390797366529 1107 24-25 0.13208474570304674 1107 26-27 0.12713110423203489 1107 28-29 0.12477686083907713 1107 30-31 0.1253030222996614 1107 32-33 0.08300622121189605 1107 34-35 0.1105266333065913 1107 36-37 0.05857595333327481 1107 38-39 0.1007670479530347 1107 40-41 0.11949224258364666 1107 42-43 0.12126347862098186 1107 44-45 0.08663177393233923 1107 46-47 0.06534552280621142 1107 48-49 0.07565332452060147 1107 50-51 0.07250764899950468 1107 52-53 0.07527981612370382 1107 54-55 0.1511757456543137 1107 56-57 0.11843914150173873 1107 58-59 0.056774699522243566 1107 60-61 0.07728616546166478 1107 62-63 0.060749816284037195 1107 64-65 0.048323642387600785 1107 66-67 0.03348303509570627 1107 68-69 3.634823139151422E-4 1107 70-71 -0.006003882468057498 1107 72-73 -0.023005296106511253 1107 74-75 0.015890983705368456 1107 76-77 0.024872134100355936 1107 78-79 -0.008230398580799658 1107 80-81 -0.005246085557182312 1107 82-83 0.0037141433192147133 1107 84-85 0.00943958754057661 1107 86-87 0.014747355046267785 1107 88-89 0.01643509974265811 1107 90-91 0.02111518284698377 1107 92-93 0.02698134220932502 1107 94-95 0.03484855662553343 1107 96-97 0.03926575002083155 1107 98-99 0.10525277589165327 1107 100 0.1100821521548383 1108 1 0.0010269327131311456 1108 2 0.06861360006825379 1108 3 0.12604491017166453 1108 4 0.058577759482979275 1108 5 0.07154682470068252 1108 6 0.0891039159690763 1108 7 0.07376213901569884 1108 8 0.04378323638962911 1108 9 0.08023454640095196 1108 10-11 -0.001862907539575076 1108 12-13 -7.232777905912258E-4 1108 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2214 100 -0.3787942679920242 2215 1 -0.3865729121390835 2215 2 -0.40030318248624397 2215 3 -0.30338510566896915 2215 4 -0.22797470643128293 2215 5 -0.21339845312002126 2215 6 -0.2334281200512791 2215 7 -0.22335580168127223 2215 8 -0.2313211940466715 2215 9 -0.3848767269356017 2215 10-11 -0.3977025608326272 2215 12-13 -0.4283257084392105 2215 14-15 -0.5288816494632798 2215 16-17 -0.5208978444109889 2215 18-19 -0.4993027611031309 2215 20-21 -0.4877593372396092 2215 22-23 -0.4540909200238872 2215 24-25 -0.47736748045529964 2215 26-27 -0.39845685570328726 2215 28-29 -0.4086046503625482 2215 30-31 -0.4138900285842624 2215 32-33 -0.4266840007755306 2215 34-35 -0.4324653507766101 2215 36-37 -0.443589201606315 2215 38-39 -0.45586277879836246 2215 40-41 -0.5652352311564073 2215 42-43 -0.4808723861628792 2215 44-45 -0.49119382076432316 2215 46-47 -0.4469718131316398 2215 48-49 -0.4850524404211143 2215 50-51 -0.5049307564657255 2215 52-53 -0.5199977586940037 2215 54-55 -0.503022385740266 2215 56-57 -0.42811471191114947 2215 58-59 -0.44399856058925735 2215 60-61 -0.4588837685662597 2215 62-63 -0.44612008323475294 2215 64-65 -0.49231343980163444 2215 66-67 -0.45949496959497793 2215 68-69 -0.4383250056962922 2215 70-71 -0.417113239128426 2215 72-73 -0.4137602844863011 2215 74-75 -0.4003602864223055 2215 76-77 -0.38676266132655 2215 78-79 -0.40146101755102137 2215 80-81 -0.41425722859752057 2215 82-83 -0.41112890937537827 2215 84-85 -0.3663552407765067 2215 86-87 -0.3179684974250705 2215 88-89 -0.29985824169961006 2215 90-91 -0.3585806149210029 2215 92-93 -0.39018572457861467 2215 94-95 -0.3544883621778929 2215 96-97 -0.340760334194222 2215 98-99 -0.37965709563640004 2215 100 -0.38302832392202646 2216 1 -0.3227659386531556 2216 2 -0.3253420946453254 2216 3 -0.27083947755946625 2216 4 -0.20500129864850436 2216 5 -0.2632273997523953 2216 6 -0.23115882058116455 2216 7 -0.30065847273143476 2216 8 -0.26028385033049517 2216 9 -0.3973912936409718 2216 10-11 -0.39710159482893914 2216 12-13 -0.448420876157563 2216 14-15 -0.5894429805772461 2216 16-17 -0.6066535494993062 2216 18-19 -0.5389175915784961 2216 20-21 -0.5554204613344922 2216 22-23 -0.529799895292193 2216 24-25 -0.52802707090936 2216 26-27 -0.5416622269263485 2216 28-29 -0.5318064922687924 2216 30-31 -0.5610322320821481 2216 32-33 -0.5094034738931512 2216 34-35 -0.5377273956110997 2216 36-37 -0.5934757745308019 2216 38-39 -0.5270125033920792 2216 40-41 -0.5946710731728189 2216 42-43 -0.6906073863529016 2216 44-45 -0.6883801140336843 2216 46-47 -0.6788438819091169 2216 48-49 -0.617416547845572 2216 50-51 -0.6561736289751892 2216 52-53 -0.6733260080034071 2216 54-55 -0.6419116939386527 2216 56-57 -0.5820468149441496 2216 58-59 -0.6161554268719129 2216 60-61 -0.6251391655599505 2216 62-63 -0.6444358059248216 2216 64-65 -0.6680080778876061 2216 66-67 -0.6435721354489559 2216 68-69 -0.6457803938933893 2216 70-71 -0.6247479275087571 2216 72-73 -0.5899383994767007 2216 74-75 -0.5854638311972238 2216 76-77 -0.5012695626034613 2216 78-79 -0.5511259726520379 2216 80-81 -0.5473436770030986 2216 82-83 -0.5506054548980117 2216 84-85 -0.6241266501503517 2216 86-87 -0.575447686625921 2216 88-89 -0.6195203208237814 2216 90-91 -0.5835634006942456 2216 92-93 -0.5642287737033769 2216 94-95 -0.5888709431098462 2216 96-97 -0.6130047288149711 2216 98-99 -0.7119975034742545 2216 100 -0.7120799710859984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 585.0 3 63.0 4 67.0 5 144.0 6 407.0 7 1147.0 8 2543.0 9 4295.0 10 5487.0 11 7506.0 12 10644.0 13 15563.0 14 18925.0 15 24644.0 16 31326.0 17 43878.0 18 46068.0 19 24610.0 20 17272.0 21 16436.0 22 16990.0 23 18817.0 24 21710.0 25 26777.0 26 34675.0 27 44170.0 28 58149.0 29 77029.0 30 100683.0 31 131055.0 32 170755.0 33 224696.0 34 308010.0 35 451474.0 36 758570.0 37 1489964.0 38 2272358.0 39 984870.0 40 3111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.84142260256058 20.96282535360513 15.399934275386132 22.795817768448163 2 15.28534092883331 21.015922232924826 40.131120961793044 23.56761587644882 3 17.602930182722513 25.882593105621037 31.32498101593831 25.18949569571814 4 12.546679895567234 18.022756227234364 38.82117047372618 30.609393403472225 5 12.50512435366159 37.84709625317978 35.92244260589929 13.725336787259343 6 29.60532757740082 38.84395824662844 17.76506113423465 13.785653041736094 7 25.70103729529261 33.29709986225923 23.044326863147184 17.957535979300975 8 24.30488999156517 36.202099987502464 20.751250456602012 18.74175956433035 9 24.422511473820883 17.022752610584753 22.217882242692458 36.3368536729019 10-11 23.181063008331822 27.45483775776833 29.191278302124996 20.17282093177485 12-13 24.139642591969725 25.1061855022448 29.338114276215315 21.416057629570158 14-15 21.41572275460644 27.109481207687715 27.194084018520996 24.280712019184854 16-17 20.25783229006387 30.30556134889243 27.938953097814434 21.497653263229267 18-19 20.132816768609302 28.57220165420195 30.971975921686408 20.32300565550234 20-21 21.141018124370685 27.79683886071251 30.822474342884902 20.2396686720319 22-23 20.86980958875613 27.646500094501715 30.88845140823629 20.595238908505863 24-25 20.744827554797933 27.776431580423637 30.813231793886338 20.665509070892092 26-27 20.54015867447381 27.732348640200026 30.87413215478778 20.853360530538385 28-29 20.518344919337327 27.710300472588944 31.089289318975506 20.682065289098226 30-31 20.853929817976706 28.020347806495316 30.62866210888446 20.497060266643523 32-33 20.551222943274993 27.88341743383172 30.86578707069197 20.699572552201314 34-35 20.769990059571576 27.646560371995182 30.817558378417548 20.76589119001569 36-37 21.004121239203464 27.817822125938974 30.3987503537954 20.779306281062166 38-39 20.810409467692136 28.019369971601265 30.487204226711423 20.68301633399518 40-41 20.8256529760405 27.731451175297266 30.504664607319587 20.938231241342645 42-43 20.834125312622522 27.821941087992684 30.329404446309027 21.014529153075767 44-45 20.970158220383357 27.960492255480663 30.085742725209773 20.983606798926203 46-47 21.11288192991924 28.02066258896121 29.743460327296074 21.122995153823474 48-49 21.14200265676401 28.05103574817028 29.46562796489921 21.3413336301665 50-51 21.16452634682357 28.127929737338814 29.242634726560528 21.46490918927709 52-53 21.173279978375113 28.173291899923825 29.203052505849257 21.4503756158518 54-55 21.254172374610423 28.461713008673396 29.051642139754573 21.232472476961604 56-57 21.19126276392668 28.15844354403264 29.085591763576137 21.564701928464547 58-59 21.0867014052559 27.892492545348436 29.37318907991496 21.6476169694807 60-61 21.173608155839556 28.176500002076228 29.135468040672034 21.514423801412182 62-63 21.02673869425286 28.14712477025903 29.2488500058871 21.577286529601007 64-65 21.05392384380735 27.95480607859676 29.291265268791406 21.70000480880448 66-67 20.997417042429863 28.023234428682546 29.177635496103193 21.80171303278439 68-69 21.32256723719984 28.142389638272082 29.013613738874948 21.521429385653125 70-71 21.266294848296955 27.936294190602524 29.0670262955877 21.730384665512823 72-73 21.081222850849503 28.029355943019286 29.136338715577697 21.753082490553513 74-75 21.162364471907168 28.04325513221032 28.965433869055175 21.828946526827337 76-77 21.198931400595782 28.036528964742086 28.837543180452197 21.926996454209934 78-79 21.284518743822396 28.05052673822543 28.92878321306634 21.73617130488584 80-81 21.085591438063332 27.938484169864676 28.994567716217905 21.981356675854087 82-83 20.9840928862336 27.98578943875128 29.101143289267796 21.928974385747324 84-85 20.98637757482053 27.868751337411457 28.99160432110734 22.15326676666067 86-87 21.051574779602785 28.000346664025162 28.870388978334372 22.07768957803768 88-89 20.965756116974017 27.86404088549154 28.88751768064971 22.282685316884727 90-91 20.842234645567085 27.789835957776283 28.917789079805296 22.450140316851332 92-93 20.887799456657618 27.93006618414663 28.793757275591314 22.388377083604443 94-95 20.724971168872415 27.733029819814746 28.97415301718696 22.567845994125875 96-97 20.74280472221677 27.786353225656192 28.88371065341207 22.58713139871497 98-99 20.669567297133966 27.809537306732263 28.820348733357946 22.70054666277582 100 20.759729999512423 27.696160508624512 28.76698419978493 22.77712529207814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 760.0 1 586.5 2 660.0 3 913.0 4 635.5 5 475.5 6 1235.0 7 3275.0 8 5679.5 9 6872.5 10 5600.0 11 3851.0 12 3717.0 13 4136.5 14 4744.0 15 5388.0 16 6159.0 17 7035.0 18 8089.0 19 9307.0 20 11027.0 21 13946.0 22 18309.5 23 24765.5 24 33286.5 25 43999.0 26 58513.0 27 76801.0 28 99071.0 29 122920.0 30 143882.0 31 164143.5 32 185012.5 33 203749.0 34 219208.0 35 232812.0 36 246893.5 37 259622.5 38 268878.0 39 272423.5 40 274843.0 41 275856.0 42 272862.0 43 270359.0 44 265457.5 45 260929.0 46 256414.0 47 248266.0 48 239762.5 49 233439.5 50 226369.5 51 213906.0 52 195723.0 53 180542.0 54 170367.0 55 160273.0 56 146544.5 57 129914.5 58 116114.0 59 104740.5 60 91119.5 61 75053.0 62 61681.0 63 50294.0 64 40043.5 65 32088.5 66 25347.5 67 19275.0 68 14684.5 69 10824.5 70 7523.5 71 5326.0 72 3920.0 73 2714.5 74 1872.0 75 1238.5 76 687.0 77 449.5 78 303.5 79 154.0 80 73.5 81 43.5 82 22.5 83 11.0 84 9.0 85 6.5 86 5.5 87 3.5 88 1.5 89 2.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13559757030800326 2 0.0 3 0.0 4 0.0 5 0.01496221337884418 6 2.1431997677843052E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.697499274325954E-6 76-77 0.0 78-79 0.0 80-81 1.6743748185814886E-4 82-83 5.291024426717503E-4 84-85 3.4157246299062365E-4 86-87 4.2194245428253506E-4 88-89 5.023124455744466E-4 90-91 6.362624310609656E-4 92-93 1.9422747895545266E-4 94-95 2.344124746014084E-4 96-97 5.157074441230984E-4 98-99 0.001131877377361086 100 6.697499274325953E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7465473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.69767760057526 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97540378324518 41.375359164742356 2 15.84193185154911 17.964014893788313 3 5.1510491183571965 8.761575666520312 4 2.2048478561110474 5.000390116164148 5 1.131157737677206 3.206700836310915 6 0.6501975571256765 2.2118814882355937 7 0.41518396391899165 1.6477976571845503 8 0.3032295947493699 1.3753931041642289 9 0.20218047961763738 1.031684728544143 >10 1.0294567333143987 10.391799459159188 >50 0.06358625891355016 2.46360845572194 >100 0.029978383894774696 3.011533424245168 >500 0.0012530320823749101 0.46682203414042356 >1k 4.4910172130958176E-4 0.35415045973500064 >5k 2.3636930556361316E-5 0.08195072701748012 >10k+ 7.091079166908395E-5 0.6553377843262106 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19571 0.26215351659566644 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19215 0.2573848971123464 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10114 0.1354770153210654 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.3394998548651908E-5 0.0 3 0.0 0.0 0.0 2.6789997097303816E-5 0.0 4 0.0 0.0 0.0 5.357999419460763E-5 0.0 5 0.0 0.0 0.0 6.697499274325953E-5 0.0 6 0.0 0.0 0.0 6.697499274325953E-5 0.0 7 0.0 0.0 0.0 6.697499274325953E-5 0.0 8 0.0 0.0 0.0 6.697499274325953E-5 0.0 9 0.0 0.0 0.0 8.036999129191145E-5 0.0 10-11 0.0 0.0 0.0 9.376498984056336E-5 0.0 12-13 0.0 6.697499274325954E-6 0.0 1.1385748766354121E-4 0.0 14-15 0.0 4.0184995645955717E-5 0.0 1.6743748185814886E-4 0.0 16-17 0.0 6.697499274325953E-5 0.0 1.875299796811267E-4 0.0 18-19 0.0 7.36724920175855E-5 0.0 1.875299796811267E-4 0.0 20-21 0.0 8.036999129191145E-5 0.0 2.1431997677843052E-4 0.0 22-23 0.0 8.036999129191145E-5 0.0 3.6166496081360146E-4 0.0 24-25 0.0 8.036999129191145E-5 0.0 7.568174179988327E-4 0.0 26-27 0.0 8.036999129191145E-5 0.0 0.0012457348650246273 0.0 28-29 0.0 8.036999129191145E-5 0.0 0.0031344296603845463 0.0 30-31 0.0 9.376498984056336E-5 0.0 0.008626379065331828 0.0 32-33 0.0 1.0715998838921526E-4 0.0 0.023180044988442124 0.0 34-35 0.0 1.0715998838921526E-4 0.0 0.047706287331023764 0.0 36-37 0.0 1.2725248621219313E-4 0.0 0.0856744107171776 0.0 38-39 0.0 1.47344984035171E-4 0.0 0.13587886527752494 0.0 40-41 0.0 1.5404248330949693E-4 0.0 0.18668609477256165 0.0 42-43 0.0 1.741349811324748E-4 0.0 0.2397570790223205 0.0 44-45 0.0 1.741349811324748E-4 0.0 0.3102415613853268 0.0 46-47 0.0 1.875299796811267E-4 0.0 0.3964115870488045 0.0 48-49 0.0 2.0762247750410456E-4 0.0 0.4884352270780431 0.0 50-51 0.0 2.2101747605275646E-4 0.0 0.580278034626875 0.0 52-53 0.0 2.2771497532708243E-4 0.0 0.6729111470900773 0.0 54-55 0.0 2.2771497532708243E-4 0.0 0.7812097103559279 0.0 56-57 0.0 2.344124746014084E-4 0.0 0.9132576060485385 0.0 58-59 0.0 2.478074731500603E-4 0.0 1.0612790375104164 0.0 60-61 0.0 2.5450497242438625E-4 0.0 1.2143972659200561 0.0 62-63 0.0 2.5450497242438625E-4 0.0 1.3665979369291135 0.0 64-65 0.0 2.5450497242438625E-4 0.0 1.5335732913373339 0.0 66-67 0.0 2.94689968070342E-4 0.0 1.7312298899212415 0.0 68-69 0.0 2.94689968070342E-4 0.0 1.9567480854863448 0.0 70-71 0.0 2.94689968070342E-4 0.0 2.199083701729281 0.0 72-73 0.0 2.94689968070342E-4 0.0 2.4477551522857293 0.0 74-75 0.0 2.94689968070342E-4 0.0 2.704858754428554 0.0 76-77 0.0 3.0808496661899386E-4 0.0 2.986709616390013 0.0 78-79 0.0 3.0808496661899386E-4 0.0 3.3043653094720185 0.0 80-81 0.0 3.0808496661899386E-4 0.0 3.6463128324220047 0.0 82-83 0.0 3.0808496661899386E-4 0.0 4.0006373340309445 0.0 84-85 0.0 3.0808496661899386E-4 0.0 4.357064850412023 0.0 86-87 0.0 3.0808496661899386E-4 0.0 4.743182381076188 0.0 88 0.0 3.0808496661899386E-4 0.0 5.057027197071103 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13240 0.0 57.484097 1 GTATCAA 23675 0.0 39.145466 1 ATCAACG 28540 0.0 31.918774 3 TCAACGC 28745 0.0 31.723846 4 TATCAAC 30270 0.0 30.389587 2 CAACGCA 30130 0.0 30.281183 5 AACGCAG 31350 0.0 29.158844 6 GTGGTAT 5700 0.0 28.240797 1 ACGCAGA 35100 0.0 25.762394 7 CGCAGAG 35380 0.0 25.3991 8 TGGTATC 6100 0.0 25.043785 2 GCAGAGT 38925 0.0 22.904823 9 GTACATG 19530 0.0 21.666222 1 CAGAGTA 37785 0.0 21.282312 10-11 GAGTACT 28225 0.0 21.155775 12-13 AGAGTAC 36140 0.0 20.31333 10-11 TACATGG 19805 0.0 20.126461 2 ACATGGG 19880 0.0 19.45942 3 GTACTTT 30340 0.0 19.456392 14-15 AGTACTT 29365 0.0 18.629929 12-13 >>END_MODULE