FastQCFastQC Report
Wed 25 May 2016
SRR2049469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4869549
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52690.10820303892619214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50250.043.991161
GTATCAA87200.035.0590821
ATCAACG93300.031.6861293
TCAACGC94500.031.2327464
CAACGCA102100.029.0932035
TATCAAC107450.028.0383072
AACGCAG106900.027.7860096
TACCTGG40150.025.519442
ACGCAGA120750.024.5211147
CGCAGAG120500.024.453228
GTACCTG46200.023.1093391
GTACATG139950.022.5839541
TACATGG140200.021.890992
ACATGGG141700.020.7968653
GCAGAGT138800.020.653829
GTGGTAT32750.020.2493671
TGGTATC31550.019.9620722
AGAGTAC121800.017.99966410-11
ACCTGGG56950.017.826263
CAGAGTA134200.017.28202610-11
Produced by FastQC (version 0.11.5)