##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049469_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4869549
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.055811739444454	31.0	31.0	34.0	28.0	34.0
2	31.251007023443034	33.0	31.0	34.0	28.0	34.0
3	31.31648023256363	33.0	31.0	34.0	28.0	34.0
4	34.8869406591863	37.0	35.0	37.0	32.0	37.0
5	34.91297797804273	37.0	35.0	37.0	32.0	37.0
6	34.91203456418654	37.0	35.0	37.0	32.0	37.0
7	34.837467905138645	37.0	35.0	37.0	32.0	37.0
8	34.81393574641101	37.0	35.0	37.0	32.0	37.0
9	36.376115529384755	39.0	37.0	39.0	32.0	39.0
10-11	36.309012087156326	39.0	37.0	39.0	32.0	39.0
12-13	36.23976111545443	39.0	37.0	39.0	32.0	39.0
14-15	37.48815680877223	40.0	38.0	41.0	32.0	41.0
16-17	37.47915012252675	40.0	38.0	41.0	32.0	41.0
18-19	37.49448039233202	40.0	38.0	41.0	32.0	41.0
20-21	37.55012178745917	40.0	38.0	41.0	32.0	41.0
22-23	37.51081927710349	40.0	38.0	41.0	32.0	41.0
24-25	37.52001140146655	40.0	38.0	41.0	32.0	41.0
26-27	37.525916260417546	40.0	38.0	41.0	32.0	41.0
28-29	37.503090224577264	40.0	38.0	41.0	32.0	41.0
30-31	37.44564014039082	40.0	38.0	41.0	32.0	41.0
32-33	37.39651361963911	40.0	38.0	41.0	32.0	41.0
34-35	37.328128025819225	40.0	38.0	41.0	31.5	41.0
36-37	37.21261856077432	40.0	37.5	41.0	31.0	41.0
38-39	37.10229910408541	40.0	37.0	41.0	31.0	41.0
40-41	36.98555923762139	40.0	37.0	41.0	30.0	41.0
42-43	36.89193023830339	40.0	37.0	41.0	30.0	41.0
44-45	36.74229512835788	40.0	37.0	41.0	30.0	41.0
46-47	36.62978984296082	40.0	37.0	41.0	30.0	41.0
48-49	36.52862410872136	40.0	36.0	41.0	30.0	41.0
50-51	36.41919600767956	40.0	36.0	41.0	30.0	41.0
52-53	36.300803318746766	40.0	36.0	41.0	30.0	41.0
54-55	36.15347694416875	39.0	35.0	41.0	29.5	41.0
56-57	35.99486800523005	39.0	35.0	41.0	29.0	41.0
58-59	35.807621917347994	39.0	35.0	41.0	29.0	41.0
60-61	35.60714000413591	39.0	35.0	41.0	28.5	41.0
62-63	35.339951400016716	38.5	35.0	40.0	28.0	41.0
64-65	35.091640724839195	38.0	34.5	40.0	28.0	41.0
66-67	34.858262335998674	37.5	34.0	40.0	28.0	41.0
68-69	34.56318295595752	37.0	34.0	40.0	28.0	41.0
70-71	34.23749026860598	36.5	34.0	39.5	27.0	41.0
72-73	33.89285496459733	36.0	34.0	39.0	26.0	41.0
74-75	33.58870113022787	35.5	34.0	39.0	26.0	40.5
76-77	33.258981786608985	35.0	33.5	38.0	26.0	40.0
78-79	32.909728601149716	35.0	33.0	37.0	26.0	39.0
80-81	32.575385523382145	35.0	33.0	37.0	25.5	39.0
82-83	32.27689381501244	35.0	33.0	36.0	25.0	39.0
84-85	31.99200921892356	35.0	33.0	36.0	25.0	37.0
86-87	31.715933960208638	35.0	33.0	36.0	24.5	37.0
88-89	31.483168667159937	35.0	33.0	35.0	24.0	37.0
90-91	31.26981769769644	35.0	32.0	35.0	23.5	36.0
92-93	31.06035179027873	35.0	32.0	35.0	22.0	36.0
94-95	30.849253493496008	35.0	32.0	35.0	20.0	36.0
96-97	30.64528480974316	35.0	32.0	35.0	18.5	36.0
98-99	30.461927069632118	35.0	32.0	35.0	9.5	35.0
100	30.316301160538686	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30646089582980096
1101	2	0.3222164449977143
1101	3	0.37075519838336746
1101	4	0.4162848011234672
1101	5	0.37009814296879995
1101	6	0.39198332459499596
1101	7	0.42798308529614104
1101	8	0.38746613534577534
1101	9	0.4955659699975712
1101	10-11	0.5284953593357429
1101	12-13	0.4699181707847444
1101	14-15	0.6076016510391042
1101	16-17	0.5375707541045216
1101	18-19	0.5409496902660109
1101	20-21	0.5244946391460203
1101	22-23	0.5359488772791821
1101	24-25	0.5682911105036084
1101	26-27	0.5402333241831698
1101	28-29	0.5468559047123875
1101	30-31	0.4972079032912049
1101	32-33	0.5178269230253036
1101	34-35	0.4952115035914062
1101	36-37	0.5144705371373917
1101	38-39	0.5824966566497949
1101	40-41	0.5872498464359097
1101	42-43	0.5500974649514632
1101	44-45	0.5975448278390161
1101	46-47	0.5266203446714641
1101	48-49	0.5513728325920724
1101	50-51	0.5064800399760827
1101	52-53	0.5356785981159717
1101	54-55	0.5470189223555337
1101	56-57	0.5322267751520471
1101	58-59	0.540981207445796
1101	60-61	0.5707687867220983
1101	62-63	0.5456620467800022
1101	64-65	0.4879791941578375
1101	66-67	0.49951281224633703
1101	68-69	0.5219612340521991
1101	70-71	0.5160975626977802
1101	72-73	0.4754461752952466
1101	74-75	0.3856442553930606
1101	76-77	0.4513284942962983
1101	78-79	0.4074404973584649
1101	80-81	0.4308003198555568
1101	82-83	0.4241021206245321
1101	84-85	0.43779059315802726
1101	86-87	0.372032952210418
1101	88-89	0.3506777254511988
1101	90-91	0.3483806048501634
1101	92-93	0.24442043527829327
1101	94-95	0.2981705830066268
1101	96-97	0.33413099556043235
1101	98-99	0.36932381810484927
1101	100	0.3580365795369538
1102	1	0.24033430078373996
1102	2	0.23826288186402067
1102	3	0.26739242117279716
1102	4	0.2581584465888582
1102	5	0.2418683791734182
1102	6	0.26553306117944686
1102	7	0.26251361205368084
1102	8	0.2982219218049593
1102	9	0.3472834954759705
1102	10-11	0.3842127685505332
1102	12-13	0.3437004482570316
1102	14-15	0.405753111622559
1102	16-17	0.34246955076250885
1102	18-19	0.40467322426516716
1102	20-21	0.3687129158125586
1102	22-23	0.39880859618538267
1102	24-25	0.39898920374488966
1102	26-27	0.31522930950671935
1102	28-29	0.3836120237205023
1102	30-31	0.29643672995049997
1102	32-33	0.2773481728929923
1102	34-35	0.290745679500489
1102	36-37	0.3207980199485192
1102	38-39	0.4282412623068552
1102	40-41	0.44417994359777424
1102	42-43	0.31366662701924497
1102	44-45	0.20555992668646184
1102	46-47	0.2602008375192426
1102	48-49	0.1843942867003605
1102	50-51	0.2637979276763218
1102	52-53	0.2488648763962047
1102	54-55	0.24970628511108828
1102	56-57	0.2795983822719492
1102	58-59	0.2721299397445449
1102	60-61	0.21070880049709473
1102	62-63	0.07730739038557743
1102	64-65	0.12408680175985154
1102	66-67	0.1653076157825808
1102	68-69	0.1917906062700041
1102	70-71	0.11378994608888604
1102	72-73	0.1250498768966608
1102	74-75	0.16165067045365333
1102	76-77	0.13363572427126513
1102	78-79	0.14520146260672107
1102	80-81	0.23463223658391286
1102	82-83	0.17191232755762798
1102	84-85	0.1378100314926911
1102	86-87	0.14040133766354046
1102	88-89	0.0666933355085284
1102	90-91	0.05423232286781854
1102	92-93	0.07737575694007504
1102	94-95	0.11858418669094561
1102	96-97	0.08307229810603545
1102	98-99	0.14321968774720517
1102	100	0.15918854080312528
1103	1	0.2088997602961591
1103	2	0.299115178561685
1103	3	0.28351363532844687
1103	4	0.2751756070220779
1103	5	0.3286364727999569
1103	6	0.29590215902925365
1103	7	0.3394814333739973
1103	8	0.36103312471266236
1103	9	0.36280787762048305
1103	10-11	0.3876706727432975
1103	12-13	0.3520198385708184
1103	14-15	0.44929027152716117
1103	16-17	0.3934215040270317
1103	18-19	0.36945352799158115
1103	20-21	0.3577510994839841
1103	22-23	0.4233111001876466
1103	24-25	0.3929422904936928
1103	26-27	0.405903810625837
1103	28-29	0.4044375247866796
1103	30-31	0.3891529070539761
1103	32-33	0.4656582670271021
1103	34-35	0.47952095761067426
1103	36-37	0.46619138482031275
1103	38-39	0.47281775765124934
1103	40-41	0.49093966792128185
1103	42-43	0.506458353362433
1103	44-45	0.41593858140000606
1103	46-47	0.3814463631864484
1103	48-49	0.38659591873256005
1103	50-51	0.3739754625708116
1103	52-53	0.42352739722085886
1103	54-55	0.36447447142529654
1103	56-57	0.3428897688336363
1103	58-59	0.2918410811199479
1103	60-61	0.3598100857444919
1103	62-63	0.3991183677033021
1103	64-65	0.3614646180689789
1103	66-67	0.32066000386190296
1103	68-69	0.27384637005528134
1103	70-71	0.3279851266044531
1103	72-73	0.38399111377061956
1103	74-75	0.3751935718728987
1103	76-77	0.3315113457525314
1103	78-79	0.3824754578694538
1103	80-81	0.39067621326023527
1103	82-83	0.3706601027239387
1103	84-85	0.2327051704255254
1103	86-87	0.26469699610763087
1103	88-89	0.23115510555792795
1103	90-91	0.26460252086641134
1103	92-93	0.25004443264980836
1103	94-95	0.2792138881686448
1103	96-97	0.27097431048609977
1103	98-99	0.1960516308129776
1103	100	0.218757020242073
1104	1	0.24087864894511668
1104	2	0.24589284982794624
1104	3	0.31540688196195177
1104	4	0.3567120204135392
1104	5	0.3349151652096083
1104	6	0.3941407542886992
1104	7	0.3563865511647606
1104	8	0.36834335349084313
1104	9	0.43374054342309876
1104	10-11	0.44784545473270043
1104	12-13	0.4621363580375899
1104	14-15	0.48337889823113045
1104	16-17	0.43066348380750696
1104	18-19	0.3849512522933196
1104	20-21	0.46241188785669607
1104	22-23	0.4476883079829008
1104	24-25	0.4472460467389041
1104	26-27	0.43597764345383183
1104	28-29	0.3699593251424034
1104	30-31	0.36533651984845505
1104	32-33	0.39717839242776876
1104	34-35	0.40797782261861215
1104	36-37	0.4011837833941314
1104	38-39	0.36656928127752053
1104	40-41	0.4041256471858219
1104	42-43	0.3560107507409924
1104	44-45	0.32707184452353744
1104	46-47	0.27190503499593177
1104	48-49	0.28390277446199974
1104	50-51	0.23995671984847178
1104	52-53	0.22492857190139404
1104	54-55	0.2646318650052706
1104	56-57	0.22384329262663272
1104	58-59	0.3189226338322442
1104	60-61	0.31320362089530107
1104	62-63	0.3100050371221883
1104	64-65	0.32414229584268384
1104	66-67	0.3657732634263553
1104	68-69	0.274370263437838
1104	70-71	0.2884001428149503
1104	72-73	0.30605178629983243
1104	74-75	0.3109218587417786
1104	76-77	0.25849345088857234
1104	78-79	0.2855824803376663
1104	80-81	0.27646515170259534
1104	82-83	0.3366966889638334
1104	84-85	0.2822170121501557
1104	86-87	0.2759194593558476
1104	88-89	0.2366738372566779
1104	90-91	0.2030240805537673
1104	92-93	0.16368013557635663
1104	94-95	0.19661530519838166
1104	96-97	0.23493502983343362
1104	98-99	0.22852863049886096
1104	100	0.17483343523383255
1105	1	0.08875209488201108
1105	2	0.07066313296470739
1105	3	0.11054732090265063
1105	4	0.10919322620271998
1105	5	0.0865675250714375
1105	6	0.12191922241991193
1105	7	0.1541114709024498
1105	8	0.1625093549142278
1105	9	0.1452469196468158
1105	10-11	0.2269788714100116
1105	12-13	0.15913916143494333
1105	14-15	0.21496581274063686
1105	16-17	0.21494158914463668
1105	18-19	0.21228223795459655
1105	20-21	0.2226553249096881
1105	22-23	0.2260564945271426
1105	24-25	0.20631113062006534
1105	26-27	0.2307478108627734
1105	28-29	0.16783819970377323
1105	30-31	0.16745137803849275
1105	32-33	0.22766116682368676
1105	34-35	0.1925591555726598
1105	36-37	0.15698076402058092
1105	38-39	0.2052432276463918
1105	40-41	0.1608065767513125
1105	42-43	0.08116422183154981
1105	44-45	0.11411869788963713
1105	46-47	0.0786440942124429
1105	48-49	0.09476465481741059
1105	50-51	0.06811046594792458
1105	52-53	0.05423076139597782
1105	54-55	-0.018902625764333436
1105	56-57	-0.013588901046809099
1105	58-59	0.03156490284864333
1105	60-61	0.06818037191084159
1105	62-63	0.18023677738182187
1105	64-65	0.17370488491486213
1105	66-67	0.10646003193244269
1105	68-69	0.05830486649334432
1105	70-71	0.13245782598202283
1105	72-73	0.11683415266426778
1105	74-75	0.12727832612934975
1105	76-77	0.06949593020199529
1105	78-79	0.09884156027290203
1105	80-81	0.12222577101351106
1105	82-83	0.07748478259036062
1105	84-85	0.05749776199469636
1105	86-87	0.0411604979898712
1105	88-89	0.04869147739876567
1105	90-91	0.02725525146929897
1105	92-93	-0.009452089173343836
1105	94-95	-0.020448296032146374
1105	96-97	0.0016999600864799902
1105	98-99	-0.023061804621438142
1105	100	-0.07229358691727938
1106	1	0.10847567232056221
1106	2	0.1102321096288783
1106	3	0.09003857491661194
1106	4	0.12748927021083745
1106	5	0.1472580104354293
1106	6	0.19626680964621812
1106	7	0.1764895730861582
1106	8	0.14648335751475372
1106	9	0.19175691579393828
1106	10-11	0.13334721034762964
1106	12-13	0.143779737935553
1106	14-15	0.21213555656700578
1106	16-17	0.17057293690098163
1106	18-19	0.14990277709038935
1106	20-21	0.2013858819402472
1106	22-23	0.16034230445495723
1106	24-25	0.20793530078024958
1106	26-27	0.2588399630936138
1106	28-29	0.22314298280607403
1106	30-31	0.27007322662722544
1106	32-33	0.2590137146559144
1106	34-35	0.19579406224049478
1106	36-37	0.24243794906652028
1106	38-39	0.21668894026555563
1106	40-41	0.22000604701907633
1106	42-43	0.16729691831348248
1106	44-45	0.10443726798891362
1106	46-47	0.13030409109250485
1106	48-49	0.08192230179283655
1106	50-51	0.04970467731266126
1106	52-53	0.07833358922359679
1106	54-55	0.12080772149558783
1106	56-57	0.050181281976854564
1106	58-59	0.0714104289719586
1106	60-61	0.07406658712363878
1106	62-63	0.09411183697451264
1106	64-65	0.05689478009517046
1106	66-67	0.10855700540054158
1106	68-69	0.12238239354607572
1106	70-71	0.12517635967478213
1106	72-73	0.1298667100181916
1106	74-75	0.09359228284103693
1106	76-77	0.010850959102945978
1106	78-79	0.04321708249520384
1106	80-81	0.09664698078174894
1106	82-83	0.12418103392305824
1106	84-85	0.09453170559655177
1106	86-87	0.11766022636316364
1106	88-89	0.11303553336852801
1106	90-91	0.04977391472898063
1106	92-93	-0.0548733687353149
1106	94-95	0.04103205090615347
1106	96-97	0.12577351392862468
1106	98-99	0.1692732901831704
1106	100	0.1373457370155613
1107	1	0.0971710707664073
1107	2	0.1282433200087567
1107	3	0.210021775800179
1107	4	0.12574598178692042
1107	5	0.10352640724863704
1107	6	0.125269465722738
1107	7	0.16130897008827105
1107	8	0.12161683301368242
1107	9	0.15302474715213776
1107	10-11	0.15328021798721636
1107	12-13	0.13037311205246027
1107	14-15	0.1683612162280781
1107	16-17	0.12360217207049118
1107	18-19	0.15149662085777038
1107	20-21	0.1327107044987983
1107	22-23	0.11472757988153148
1107	24-25	0.1683117230349751
1107	26-27	0.24320364292903918
1107	28-29	0.15363295217569828
1107	30-31	0.17051416698619448
1107	32-33	0.13460394867514225
1107	34-35	0.1949880545465561
1107	36-37	0.18590345516540907
1107	38-39	0.2496071275688294
1107	40-41	0.22559556458702446
1107	42-43	0.18492689955037633
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2216	88-89	-0.7540558073043826
2216	90-91	-0.8579290830871784
2216	92-93	-0.8297755554358233
2216	94-95	-0.7872313761528282
2216	96-97	-0.7878190452383436
2216	98-99	-0.7945283766092004
2216	100	-0.876593384229583
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71545.0
3	12969.0
4	8218.0
5	6807.0
6	5958.0
7	6403.0
8	7119.0
9	7927.0
10	9055.0
11	9985.0
12	11400.0
13	12607.0
14	13921.0
15	14447.0
16	14506.0
17	15041.0
18	14353.0
19	13456.0
20	13805.0
21	14863.0
22	16692.0
23	20107.0
24	24633.0
25	29784.0
26	36041.0
27	40953.0
28	49212.0
29	62609.0
30	75679.0
31	88719.0
32	114370.0
33	154161.0
34	214946.0
35	316333.0
36	531421.0
37	979305.0
38	1326767.0
39	502365.0
40	1067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.766817200619286	20.3681213851864	13.865716424944338	22.999344989249973
2	16.081288994056298	20.759168847023663	39.86938602146585	23.290156137454197
3	18.01814310649452	25.928773333585287	30.602494420397047	25.450589139523146
4	12.74938693021479	17.80685323704954	39.02173779097709	30.422022041758577
5	12.716116118500908	37.36617960142337	35.31316119486731	14.604543085208418
6	29.394663178064523	38.42070241662371	18.5874447290001	13.597189676311666
7	26.142689478075436	32.62635466189373	23.145210553104224	18.085745306926608
8	24.419268063963997	35.586967156653145	21.48754372972167	18.506221049661193
9	24.448699867593085	17.48373050273758	22.362454023664835	35.7051156060045
10-11	23.084766426564375	27.2287234665264	29.48906086943306	20.197449237476167
12-13	23.872284284456573	25.091534107847835	29.122988588492866	21.91319301920273
14-15	21.582047716367605	27.510929720975064	26.445550201342638	24.461472361314694
16-17	20.864825786628863	30.480354367439027	26.909353799042197	21.745466046889913
18-19	20.69794174179836	29.34320534914236	28.96775739831267	20.99109551074661
20-21	22.146270790882788	28.866976346407537	28.15289322990958	20.833859632800092
22-23	21.71419786759644	28.909016297820113	28.201172674081388	21.175613160502063
24-25	21.41355955631874	28.858126356670905	28.383426969321075	21.34488711768928
26-27	21.259962183390634	28.667029211040873	28.45066292019225	21.622345685376242
28-29	21.310559771614006	28.74725556844905	28.515429404697112	21.426755255239836
30-31	21.660030864387696	29.322154132168095	27.94955352613512	21.06826147730909
32-33	21.098028221661526	28.96194357926906	28.492766928427148	21.44726127064226
34-35	21.28810485651441	28.404273453368468	28.5767169905096	21.730904699607528
36-37	21.86977340551611	28.926756128044843	27.900370679695136	21.303099786743907
38-39	21.60697069022108	28.953928161074177	28.17291533072862	21.266185817976123
40-41	21.36007664104537	28.685944749890197	28.35680682712419	21.597171781940247
42-43	21.23674317670038	28.723152234063342	28.298601181090678	21.7415034081456
44-45	21.560312356604797	28.876256075232444	28.112987923684685	21.450443644478074
46-47	21.437944071793993	29.204189454885103	27.92167853425563	21.436187939065277
48-49	21.265843766274326	28.743260356234607	28.249450286612976	21.74144559087809
50-51	21.336038587455626	28.586528339703683	28.232167159730654	21.84526591311004
52-53	21.325424123910263	28.650654163091758	28.17918230768796	21.844739405310015
54-55	21.50107358468226	29.137978608816223	27.926027864012674	21.434919942488843
56-57	21.21574962617283	28.854476477644315	27.977060979016382	21.952712917166473
58-59	21.075648959093282	28.362083261242454	28.404542007718558	22.157725771945707
60-61	21.505334054877768	28.854151156713087	27.882479306085106	21.758035482324047
62-63	21.332145497863948	28.82398126848505	27.96803113703582	21.875842096615184
64-65	21.333585162845985	28.515080911014827	28.193289626739777	21.95804429939941
66-67	21.168270443325877	28.605437258827614	27.969126238158577	22.25716605968793
68-69	21.72591381573137	28.809662200880286	27.822893196984104	21.641530786404235
70-71	21.707628694028546	28.431484718579057	28.007047591268464	21.85383899612393
72-73	21.378450287626027	28.55158551453032	28.31627987034774	21.753684327495915
74-75	21.397977947312015	28.508416490066125	28.163202009626037	21.930403552995827
76-77	21.50754871072574	28.618250298593583	27.822811984983186	22.051389005697487
78-79	21.62311868337565	28.6927985373579	27.960501926782865	21.723580852483583
80-81	21.41454638107927	28.490718710804195	28.11026939960282	21.984465508513715
82-83	21.318650150242203	28.507365467224442	28.225755685774708	21.948228696758648
84-85	21.430707392097386	28.359198003202714	28.101588479105654	22.108506125594243
86-87	21.66745777075426	28.49555211910803	27.952740379766933	21.88424973037078
88-89	21.36068325300596	28.374109076808253	28.068018861220345	22.197188808965446
90-91	21.314511794338628	28.219974905618795	28.0854453615959	22.380067938446675
92-93	21.531388444795315	28.419819736744955	27.85521475844944	22.19357706001029
94-95	21.43433259228953	28.131125729336624	28.11975744371107	22.314784234662778
96-97	21.425308730595418	28.18027890962394	28.089755178234704	22.304657181545934
98-99	21.41847342164528	28.17611510874109	28.005712800536365	22.39969866907726
100	21.53186654801603	28.23950232451059	27.894763756734424	22.333867370738954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3893.0
1	2727.0
2	1885.0
3	2221.5
4	2053.5
5	1764.0
6	1582.0
7	1566.5
8	1643.5
9	1658.0
10	1478.5
11	1222.5
12	1156.5
13	1263.5
14	1319.5
15	1424.5
16	1633.0
17	1928.0
18	2466.0
19	3128.0
20	4244.0
21	5968.5
22	8637.5
23	12649.0
24	18012.5
25	25126.5
26	34649.5
27	46519.5
28	61009.0
29	77072.5
30	91644.5
31	105158.5
32	118330.0
33	130218.0
34	140781.5
35	149514.5
36	156656.0
37	163120.0
38	169495.5
39	172557.5
40	174022.5
41	174538.0
42	171731.0
43	168696.5
44	165479.0
45	162435.5
46	159886.5
47	156345.0
48	154373.0
49	153371.0
50	149572.5
51	142810.0
52	133138.0
53	125110.0
54	120129.0
55	115320.5
56	107128.0
57	96814.5
58	88091.0
59	79628.0
60	69788.0
61	58745.5
62	48299.5
63	38841.0
64	30812.5
65	24162.5
66	18592.5
67	13975.5
68	10503.5
69	7854.0
70	5742.5
71	4271.5
72	3227.0
73	2373.0
74	1689.5
75	1219.5
76	796.5
77	586.0
78	464.5
79	316.5
80	229.0
81	170.5
82	134.5
83	118.0
84	94.5
85	81.5
86	78.5
87	59.0
88	42.5
89	30.0
90	30.0
91	28.5
92	15.5
93	13.5
94	17.0
95	15.0
96	9.0
97	8.0
98	7.5
99	12.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08173241505527513
2	0.014333976308688955
3	0.01895452741106004
4	0.01250629165041773
5	0.010740214340178115
6	0.007372345981116527
7	0.020350960633109965
8	0.008933065464584092
9	0.00961074629293185
10-11	0.013389330305537536
12-13	0.015104068159084138
14-15	0.009980390381121538
16-17	0.012146915453566645
18-19	0.008871458116552478
20-21	0.013307187174828716
22-23	0.013902724872467655
24-25	0.016089785727589966
26-27	0.00533930349607325
28-29	0.012300933823645681
30-31	0.010411641817342839
32-33	0.012198254910259656
34-35	0.008604492941748816
36-37	0.010688874883485104
38-39	0.019200956803186495
40-41	0.01268084580317397
42-43	0.00832725987560655
44-45	0.02076167628665406
46-47	0.01841032917011411
48-49	0.01797907773389281
50-51	0.01038083814332703
52-53	0.011120126319706405
54-55	0.011921021844117391
56-57	0.020309889067755556
58-59	0.0023924186818943603
60-61	0.007475024894502551
62-63	0.015381301225226402
64-65	0.024150080428392857
66-67	0.012834864173253004
68-69	0.013943796437822066
70-71	0.02503311908351266
72-73	0.020279085393739748
74-75	0.02015587069767652
76-77	0.009703157314979272
78-79	0.011335752037817054
80-81	0.019786226609486833
82-83	0.011859414496085777
84-85	0.011335752037817054
86-87	0.016962556491371173
88-89	0.014847370875619076
90-91	0.01499112135435951
92-93	0.008060294700802887
94-95	0.015217014963808763
96-97	0.013553616566955173
98-99	0.009929050924428525
100	0.007228595502376092
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4869549.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.54295352189843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85022365653668	55.41713122325087
2	12.469053445412188	17.0933150154191
3	3.262698139280783	6.709049007501217
4	1.2260801591961772	3.3615662146362157
5	0.6409317649121722	2.1965678086541676
6	0.358004339995953	1.4723204902188318
7	0.2323348854732463	1.1147443479555823
8	0.1609040815853602	0.8823072788471279
9	0.11347075919290704	0.6999858876108562
>10	0.6388287778566805	7.706884313726546
>50	0.03363478283774387	1.578727158028758
>100	0.013655221567207242	1.573343728151806
>500	8.999312497331478E-5	0.03927211160683124
>1k	5.9995351922965566E-5	0.04644796109460701
>5k	2.999767596039435E-5	0.10833745329744218
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5269	0.10820303892619214	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.0535782677204808E-5	0.0	0.0	0.0
4	0.0	2.0535782677204808E-5	0.0	2.0535782677204808E-5	0.0
5	0.0	2.0535782677204808E-5	0.0	2.0535782677204808E-5	0.0
6	0.0	2.0535782677204808E-5	0.0	2.0535782677204808E-5	0.0
7	0.0	2.0535782677204808E-5	0.0	2.0535782677204808E-5	0.0
8	0.0	2.0535782677204808E-5	0.0	2.0535782677204808E-5	0.0
9	0.0	2.0535782677204808E-5	0.0	4.1071565354409616E-5	0.0
10-11	0.0	2.0535782677204808E-5	0.0	4.1071565354409616E-5	0.0
12-13	0.0	2.0535782677204808E-5	0.0	4.1071565354409616E-5	0.0
14-15	0.0	3.0803674015807214E-5	0.0	4.1071565354409616E-5	0.0
16-17	0.0	5.1339456693012025E-5	0.0	4.1071565354409616E-5	0.0
18-19	0.0	8.214313070881925E-5	0.0	7.187523937021683E-5	0.0
20-21	0.0	1.0267891338602405E-4	0.0	9.241102204742165E-5	0.0
22-23	0.0	1.0267891338602405E-4	0.0	3.4910830551248173E-4	0.0
24-25	0.0	1.0267891338602405E-4	0.0	9.65181785828626E-4	0.0
26-27	0.0	1.2321469606322885E-4	0.0	0.001930363571657252	0.0
28-29	0.0	1.2321469606322885E-4	0.0	0.0042714427968586	0.0
30-31	0.0	1.2321469606322885E-4	0.0	0.012249594366952669	0.0
32-33	0.0	1.2321469606322885E-4	0.0	0.029756349099269767	0.0
34-35	0.0	1.2321469606322885E-4	0.0	0.06218234994657616	0.0
36-37	0.0	1.2321469606322885E-4	0.0	0.1093530427561156	0.0
38-39	0.0	1.2321469606322885E-4	0.0	0.168999223542057	0.0
40-41	0.0	1.2321469606322885E-4	0.0	0.23554542730753916	0.0
42-43	1.0267891338602404E-5	1.2321469606322885E-4	0.0	0.30672245006673104	0.0
44-45	2.0535782677204808E-5	1.2321469606322885E-4	0.0	0.39533435231886976	0.0
46-47	2.0535782677204808E-5	1.2321469606322885E-4	0.0	0.49935835947025076	0.0
48-49	4.1071565354409616E-5	1.2321469606322885E-4	0.0	0.6100667638830619	0.0
50-51	4.1071565354409616E-5	2.669651748036625E-4	0.0	0.725087682658086	0.0
52-53	4.1071565354409616E-5	2.875009574808673E-4	0.0	0.8415255704378373	0.0
54-55	4.1071565354409616E-5	3.080367401580721E-4	0.0	0.975819321255418	0.0
56-57	4.1071565354409616E-5	3.388404141738793E-4	0.0	1.1370970905108462	0.0
58-59	4.1071565354409616E-5	3.4910830551248173E-4	0.0	1.3118668689851976	0.0
60-61	4.1071565354409616E-5	3.4910830551248173E-4	0.0	1.4909183581477463	0.0
62-63	4.1071565354409616E-5	3.4910830551248173E-4	0.0	1.6752886150236912	0.0
64-65	4.1071565354409616E-5	3.4910830551248173E-4	0.0	1.8778843790256552	0.0
66-67	4.1071565354409616E-5	3.9017987086689134E-4	0.0	2.120699473400925	0.0
68-69	6.160734803161443E-5	3.9017987086689134E-4	0.0	2.3837628494959184	0.0
70-71	6.160734803161443E-5	3.9017987086689134E-4	0.0	2.6635526205814952	0.0
72-73	6.160734803161443E-5	4.31251436221301E-4	0.0	2.9645147836072705	0.0
74-75	6.160734803161443E-5	4.517872188985058E-4	0.0	3.2653229282629663	0.0
76-77	6.160734803161443E-5	4.723230015757106E-4	0.0	3.593412860205329	0.0
78-79	6.160734803161443E-5	4.723230015757106E-4	0.0	3.958918988185559	0.0
80-81	6.160734803161443E-5	4.723230015757106E-4	0.0	4.352086815431983	0.0
82-83	6.160734803161443E-5	4.723230015757106E-4	0.0	4.759978798857964	0.0
84-85	6.160734803161443E-5	5.031266755915178E-4	0.0	5.171094900164266	0.0
86-87	6.160734803161443E-5	5.236624582687226E-4	0.0	5.61569459512575	0.0
88	6.160734803161443E-5	5.544661322845299E-4	0.0	5.9659118329028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5025	0.0	43.99116	1
GTATCAA	8720	0.0	35.059082	1
ATCAACG	9330	0.0	31.686129	3
TCAACGC	9450	0.0	31.232746	4
CAACGCA	10210	0.0	29.093203	5
TATCAAC	10745	0.0	28.038307	2
AACGCAG	10690	0.0	27.786009	6
TACCTGG	4015	0.0	25.51944	2
ACGCAGA	12075	0.0	24.521114	7
CGCAGAG	12050	0.0	24.45322	8
GTACCTG	4620	0.0	23.109339	1
GTACATG	13995	0.0	22.583954	1
TACATGG	14020	0.0	21.89099	2
ACATGGG	14170	0.0	20.796865	3
GCAGAGT	13880	0.0	20.65382	9
GTGGTAT	3275	0.0	20.249367	1
TGGTATC	3155	0.0	19.962072	2
AGAGTAC	12180	0.0	17.999664	10-11
ACCTGGG	5695	0.0	17.82626	3
CAGAGTA	13420	0.0	17.282026	10-11
>>END_MODULE