FastQCFastQC Report
Wed 25 May 2016
SRR2049469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4869549
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164850.3385323774337213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158540.32557429856440506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91790.18849794919406296No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.10493784948051657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91150.057.824561
GTATCAA168450.040.4527931
ATCAACG207250.032.6358643
TCAACGC210200.032.2449264
TATCAAC216650.031.3283422
CAACGCA217950.031.0552145
AACGCAG229100.029.6846686
TATAACG4350.029.17452
GTGGTAT37100.028.4135251
TACCTGG37850.027.941232
TGGTATC39100.026.2064742
ACGCAGA259500.026.0803787
CGCAGAG261900.025.661938
GTACCTG45100.024.1038511
GCAGAGT285450.023.3307429
GTACATG132450.023.0947041
GTACACG5150.021.9308261
TACATGG135600.021.6645282
ATAACGC5900.021.5100123
TAACGCA7400.020.9609574
Produced by FastQC (version 0.11.5)