##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049469_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4869549
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.925522876964582	33.0	31.0	34.0	30.0	34.0
2	32.092126601457345	33.0	31.0	34.0	30.0	34.0
3	32.1102297153186	34.0	31.0	34.0	30.0	34.0
4	35.7943203775134	37.0	35.0	37.0	33.0	37.0
5	35.85909537002297	37.0	35.0	37.0	35.0	37.0
6	35.9335494929818	37.0	35.0	37.0	35.0	37.0
7	35.89864092136664	37.0	35.0	37.0	35.0	37.0
8	35.91942703523468	37.0	35.0	37.0	35.0	37.0
9	37.64106429568734	39.0	37.0	39.0	35.0	39.0
10-11	37.61232292764689	39.0	37.0	39.0	35.0	39.0
12-13	37.59382665622628	39.0	37.0	39.0	35.0	39.0
14-15	38.880019073634955	40.0	38.0	41.0	35.0	41.0
16-17	38.889561846487226	40.0	38.0	41.0	35.0	41.0
18-19	38.969072392535736	40.0	38.5	41.0	35.0	41.0
20-21	38.99117372060534	40.0	39.0	41.0	35.0	41.0
22-23	38.94857460105648	40.0	38.5	41.0	35.0	41.0
24-25	38.92256315728623	40.0	38.0	41.0	35.0	41.0
26-27	38.82088289901179	40.0	38.0	41.0	35.0	41.0
28-29	38.73085402775493	40.0	38.0	41.0	35.0	41.0
30-31	38.605846660542895	40.0	38.0	41.0	35.0	41.0
32-33	38.45198744277961	40.0	38.0	41.0	34.0	41.0
34-35	38.31592330213742	40.0	38.0	41.0	34.0	41.0
36-37	38.16995968209787	40.0	38.0	41.0	34.0	41.0
38-39	38.053545821183846	40.0	38.0	41.0	34.0	41.0
40-41	37.957626055308204	40.0	38.0	41.0	33.0	41.0
42-43	37.805720714587736	40.0	38.0	41.0	33.0	41.0
44-45	37.618388889813005	40.0	37.0	41.0	33.0	41.0
46-47	37.348240257978716	40.0	37.0	41.0	32.5	41.0
48-49	37.083067240929296	40.0	37.0	41.0	31.5	41.0
50-51	36.9197033441906	40.0	37.0	41.0	31.0	41.0
52-53	36.797258226583196	40.0	36.5	41.0	31.0	41.0
54-55	36.65312721978976	40.0	36.0	41.0	31.0	41.0
56-57	36.50209054267654	39.5	36.0	41.0	31.0	41.0
58-59	36.32477330036108	39.0	35.0	41.0	30.5	41.0
60-61	36.12911822018836	39.0	35.0	41.0	30.0	41.0
62-63	35.90092172806968	39.0	35.0	41.0	30.0	41.0
64-65	35.67209283652346	38.5	35.0	40.5	30.0	41.0
66-67	35.40402417143764	38.0	35.0	40.0	29.0	41.0
68-69	35.102239344957816	37.0	35.0	40.0	29.0	41.0
70-71	34.80803725355264	37.0	34.5	39.5	29.0	41.0
72-73	34.471858482171555	36.0	34.0	39.0	29.0	41.0
74-75	34.14770679995211	36.0	34.0	39.0	28.5	41.0
76-77	33.828362647136316	35.0	34.0	38.5	28.0	40.0
78-79	33.5203846393167	35.0	34.0	37.0	28.0	39.0
80-81	33.21154484737704	35.0	34.0	37.0	28.0	39.0
82-83	32.937911703938084	35.0	34.0	36.5	27.0	39.0
84-85	32.654315317496554	35.0	34.0	36.0	27.0	37.0
86-87	32.409998338655186	35.0	33.5	36.0	26.5	37.0
88-89	32.18852711000547	35.0	33.0	35.0	26.0	37.0
90-91	31.998086886485794	35.0	33.0	35.0	26.0	36.0
92-93	31.832673929351568	35.0	33.0	35.0	26.0	36.0
94-95	31.666937430961266	35.0	33.0	35.0	25.5	36.0
96-97	31.517275624498286	35.0	33.0	35.0	25.0	36.0
98-99	31.37676928602628	35.0	33.0	35.0	25.0	35.0
100	31.264861899941863	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.349008703652963
1101	2	0.3576044825620457
1101	3	0.5090394932124482
1101	4	0.3216357676720847
1101	5	0.2444328483411411
1101	6	0.262019336968514
1101	7	0.27302997999010614
1101	8	0.28625033744209105
1101	9	0.39692408110841626
1101	10-11	0.3414173169244634
1101	12-13	0.32214807039854065
1101	14-15	0.4520974112407785
1101	16-17	0.43884278641539964
1101	18-19	0.4329708061546427
1101	20-21	0.4718203473831011
1101	22-23	0.447098877396364
1101	24-25	0.4368312368807139
1101	26-27	0.38607860469687694
1101	28-29	0.43902088310185405
1101	30-31	0.49494921256494706
1101	32-33	0.4694219292978872
1101	34-35	0.48785969973648236
1101	36-37	0.4617238818300109
1101	38-39	0.43818533917568203
1101	40-41	0.49674501743972854
1101	42-43	0.5380225830213874
1101	44-45	0.4981674060119943
1101	46-47	0.4242718234450962
1101	48-49	0.46536156582626376
1101	50-51	0.4623955261752144
1101	52-53	0.41117236674591595
1101	54-55	0.45634412058220875
1101	56-57	0.46391672592572775
1101	58-59	0.46249680476181254
1101	60-61	0.3870371233759897
1101	62-63	0.40027187651718066
1101	64-65	0.4250465621656687
1101	66-67	0.4274902837522987
1101	68-69	0.46726504988902917
1101	70-71	0.406828775701058
1101	72-73	0.4293473774624488
1101	74-75	0.45694252257985823
1101	76-77	0.43920670416491703
1101	78-79	0.4043435775941049
1101	80-81	0.410590324643735
1101	82-83	0.38774154501980007
1101	84-85	0.38674782995236967
1101	86-87	0.37057940996208316
1101	88-89	0.3744440542369887
1101	90-91	0.3631173673379777
1101	92-93	0.3616988987669636
1101	94-95	0.37420857252926254
1101	96-97	0.38376737859128696
1101	98-99	0.43340505172783494
1101	100	0.44355986375281375
1102	1	0.23420648426490231
1102	2	0.24211829847666877
1102	3	0.29543849947031475
1102	4	0.19627893450683587
1102	5	0.222152070797911
1102	6	0.19207925729622843
1102	7	0.19012221512051042
1102	8	0.19764264801532505
1102	9	0.1939666214759086
1102	10-11	0.19751112000032833
1102	12-13	0.18669527956199516
1102	14-15	0.26187591621601314
1102	16-17	0.2769604091483657
1102	18-19	0.28876912412209776
1102	20-21	0.28184756679372214
1102	22-23	0.22982573641988324
1102	24-25	0.29140424797937925
1102	26-27	0.2214220470397379
1102	28-29	0.23049843797755187
1102	30-31	0.24751075113606902
1102	32-33	0.26011474583383176
1102	34-35	0.31083724136436075
1102	36-37	0.30214774580509385
1102	38-39	0.28356813860923324
1102	40-41	0.30552870568832446
1102	42-43	0.25275889691212683
1102	44-45	0.2714254390630657
1102	46-47	0.23809435787527633
1102	48-49	0.24506619993454848
1102	50-51	0.20161101697210881
1102	52-53	0.17516191800530123
1102	54-55	0.23090328340548893
1102	56-57	0.2929600724823658
1102	58-59	0.2909097794414208
1102	60-61	0.21861387910890784
1102	62-63	0.1928226139475484
1102	64-65	0.18402737218997345
1102	66-67	0.1666459884769722
1102	68-69	0.19555070880851844
1102	70-71	0.1697370507961793
1102	72-73	0.15687089725951608
1102	74-75	0.1626482958636828
1102	76-77	0.13065838683318276
1102	78-79	0.15926392485479823
1102	80-81	0.15225219097050058
1102	82-83	0.11584826954338467
1102	84-85	0.09084665259740632
1102	86-87	0.11240791988235088
1102	88-89	0.07223690229152879
1102	90-91	0.12081709021114762
1102	92-93	0.12219810823184574
1102	94-95	0.09092992620296769
1102	96-97	0.1256794769052405
1102	98-99	0.21590241863874482
1102	100	0.23271671279357165
1103	1	0.28722540607409286
1103	2	0.24527130835470956
1103	3	0.36846037104374574
1103	4	0.2435051876051375
1103	5	0.2529252547979013
1103	6	0.22369081984448513
1103	7	0.24130543497050638
1103	8	0.21813582863159553
1103	9	0.28092011629353664
1103	10-11	0.28158012503574525
1103	12-13	0.28631011886746904
1103	14-15	0.37413669947797956
1103	16-17	0.3384927472772077
1103	18-19	0.3361576194260465
1103	20-21	0.32113064994751994
1103	22-23	0.3170310965185692
1103	24-25	0.3121126351811725
1103	26-27	0.31560495988770754
1103	28-29	0.3502447846314354
1103	30-31	0.32922193611398853
1103	32-33	0.35892429639770995
1103	34-35	0.3094462022702018
1103	36-37	0.2812842024523903
1103	38-39	0.2447576099629103
1103	40-41	0.2700360152770429
1103	42-43	0.26217488005683975
1103	44-45	0.3138229855088568
1103	46-47	0.24966751452412694
1103	48-49	0.206830249466897
1103	50-51	0.20590171362620424
1103	52-53	0.24025488225897362
1103	54-55	0.17174119179143332
1103	56-57	0.14766121851207004
1103	58-59	0.16452773045594427
1103	60-61	0.14657212010909149
1103	62-63	0.2112275937164796
1103	64-65	0.1635684063562195
1103	66-67	0.20362858654893756
1103	68-69	0.17084923925127526
1103	70-71	0.20328015807307054
1103	72-73	0.22137367591104606
1103	74-75	0.20258442102844754
1103	76-77	0.15435883478304646
1103	78-79	0.17404723239601338
1103	80-81	0.1402671509251121
1103	82-83	0.17964355476703986
1103	84-85	0.1729635917931347
1103	86-87	0.1847402200568382
1103	88-89	0.17685541431985996
1103	90-91	0.17578197454280797
1103	92-93	0.1543363275740539
1103	94-95	0.12581295729236075
1103	96-97	0.15833903850034403
1103	98-99	0.22396722821253334
1103	100	0.20753445183677854
1104	1	0.26538480029617517
1104	2	0.26485308475250235
1104	3	0.3108813837475992
1104	4	0.243587434015339
1104	5	0.22740723278064934
1104	6	0.17020804818751145
1104	7	0.1727480678083424
1104	8	0.19812596342814714
1104	9	0.23334028907017768
1104	10-11	0.24580702345376437
1104	12-13	0.16827934095589114
1104	14-15	0.30519693865520026
1104	16-17	0.31618868989031057
1104	18-19	0.3247244831259408
1104	20-21	0.30564040810450877
1104	22-23	0.2832179867997482
1104	24-25	0.28482344120738645
1104	26-27	0.3182304539998171
1104	28-29	0.315953379657806
1104	30-31	0.33356865040385486
1104	32-33	0.3554138226654189
1104	34-35	0.38181118287899807
1104	36-37	0.39669786612054736
1104	38-39	0.37379992340041923
1104	40-41	0.37136942580379895
1104	42-43	0.3845057918304491
1104	44-45	0.32872299839517893
1104	46-47	0.2636897513242715
1104	48-49	0.3359079499620492
1104	50-51	0.3125668550193268
1104	52-53	0.31811965194003733
1104	54-55	0.2769562520865918
1104	56-57	0.3034184199298835
1104	58-59	0.3244906072738303
1104	60-61	0.291869765889885
1104	62-63	0.3044976666004757
1104	64-65	0.20453253947529504
1104	66-67	0.27627856758326885
1104	68-69	0.19823296444999272
1104	70-71	0.27573599537681304
1104	72-73	0.28186114463601086
1104	74-75	0.2715494063474253
1104	76-77	0.2749033671394443
1104	78-79	0.3138767652507113
1104	80-81	0.3141544226030959
1104	82-83	0.2629679847425379
1104	84-85	0.21911554275147438
1104	86-87	0.17391221699506332
1104	88-89	0.23884959798761685
1104	90-91	0.20476684889194985
1104	92-93	0.2395269094359911
1104	94-95	0.2155519160149737
1104	96-97	0.23116357789922404
1104	98-99	0.22312676740270732
1104	100	0.20671024142353644
1105	1	0.19436737161620954
1105	2	0.18927901663013103
1105	3	0.2695400673874957
1105	4	0.13703270415112456
1105	5	0.16224303236296578
1105	6	0.15277801077889563
1105	7	0.13227735602536939
1105	8	0.13568046968227065
1105	9	0.1966832808839385
1105	10-11	0.15950464053495494
1105	12-13	0.17013444884057094
1105	14-15	0.24128723479856973
1105	16-17	0.1991587049480401
1105	18-19	0.22494795287217073
1105	20-21	0.22719305728359984
1105	22-23	0.17576030378452856
1105	24-25	0.18864272182710096
1105	26-27	0.15061815906612708
1105	28-29	0.2522582324339453
1105	30-31	0.2806384229416139
1105	32-33	0.24287734452919807
1105	34-35	0.2663152588798354
1105	36-37	0.3211091767188776
1105	38-39	0.2652641309803414
1105	40-41	0.30539960555075396
1105	42-43	0.26585217766763236
1105	44-45	0.2586821699394548
1105	46-47	0.28014803159171464
1105	48-49	0.3385492593347692
1105	50-51	0.2705324716143238
1105	52-53	0.24555978757949504
1105	54-55	0.23031703998741904
1105	56-57	0.22135291793305356
1105	58-59	0.23643853692944106
1105	60-61	0.27563636112816425
1105	62-63	0.26520244355302935
1105	64-65	0.23049113113802377
1105	66-67	0.19059229155316615
1105	68-69	0.20146610777725016
1105	70-71	0.27776058816153437
1105	72-73	0.3041340810575619
1105	74-75	0.32587263564435887
1105	76-77	0.24818764399087456
1105	78-79	0.15208136970438346
1105	80-81	0.21225587421889003
1105	82-83	0.15497244341596428
1105	84-85	0.16483521097659093
1105	86-87	0.16921727934671082
1105	88-89	0.1929524244408043
1105	90-91	0.17935856260919536
1105	92-93	0.20585486245297702
1105	94-95	0.13600784270509791
1105	96-97	0.17561218414849122
1105	98-99	0.16931002566288456
1105	100	0.1735895121513984
1106	1	0.11048377268381415
1106	2	0.09029380436857792
1106	3	0.19239429847388578
1106	4	0.13921794809333932
1106	5	0.13220816393946677
1106	6	0.12525510410405616
1106	7	0.11082569452977964
1106	8	0.0604250826016397
1106	9	0.13223736763514182
1106	10-11	0.09959619503390371
1106	12-13	0.06878245235695601
1106	14-15	0.08924721214076925
1106	16-17	0.12162856729248261
1106	18-19	0.11711037929612189
1106	20-21	0.16051880957360964
1106	22-23	0.18672031547662016
1106	24-25	0.1754571871141266
1106	26-27	0.15452838025974813
1106	28-29	0.16708461997819057
1106	30-31	0.15244929242930283
1106	32-33	0.13618207993547315
1106	34-35	0.09739293098975565
1106	36-37	0.14045095618774894
1106	38-39	0.16654753707199887
1106	40-41	0.17566483923613418
1106	42-43	0.11599518918184515
1106	44-45	0.10544084181068314
1106	46-47	0.0558767580136319
1106	48-49	0.03984290475766272
1106	50-51	0.0017066516090764594
1106	52-53	0.015421760081643754
1106	54-55	0.049752275232769705
1106	56-57	0.10389772430576727
1106	58-59	0.03005512342083705
1106	60-61	0.006036694912864959
1106	62-63	-0.02440430705426877
1106	64-65	-0.022637225223959945
1106	66-67	-0.04481720840209391
1106	68-69	0.019641752127647294
1106	70-71	0.008252080436854214
1106	72-73	0.07842747421658913
1106	74-75	0.08668586170976056
1106	76-77	0.025525903580238207
1106	78-79	0.03671986770825697
1106	80-81	-1.3628735425186278E-4
1106	82-83	0.03146496268745835
1106	84-85	0.012671080185320704
1106	86-87	0.03592418133420949
1106	88-89	0.019306581467070316
1106	90-91	0.04445155564665981
1106	92-93	0.12631331975770266
1106	94-95	0.09499737386406082
1106	96-97	0.07468236316832133
1106	98-99	0.04116544061725236
1106	100	0.058687806449469804
1107	1	0.06644074228649544
1107	2	0.11796250729808833
1107	3	0.19862407148171002
1107	4	0.08965228627042876
1107	5	0.14106552202952827
1107	6	0.10472575490631897
1107	7	0.08467305666653857
1107	8	0.10550763382634187
1107	9	0.10735080749061865
1107	10-11	0.087308659807519
1107	12-13	0.05068021725804783
1107	14-15	0.1302038370554044
1107	16-17	0.0848277138823832
1107	18-19	0.09117313501351276
1107	20-21	0.08049248207164794
1107	22-23	0.1203791447077478
1107	24-25	0.12528942504760465
1107	26-27	0.13478883529379004
1107	28-29	0.1575755723856318
1107	30-31	0.1407012512675081
1107	32-33	0.13635820407148458
1107	34-35	0.11616536044481762
1107	36-37	0.10857540335914706
1107	38-39	0.14580782403156434
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2216	68-69	-0.7137237449407721
2216	70-71	-0.6909381910302699
2216	72-73	-0.6216463029762878
2216	74-75	-0.61286098507383
2216	76-77	-0.5153213030730583
2216	78-79	-0.5638191999595534
2216	80-81	-0.5620899698418356
2216	82-83	-0.5028722519677657
2216	84-85	-0.6401425097296567
2216	86-87	-0.5987266080644105
2216	88-89	-0.6371092600827488
2216	90-91	-0.6144677688628839
2216	92-93	-0.6311763657399183
2216	94-95	-0.7062546276999129
2216	96-97	-0.700765479997493
2216	98-99	-0.758674564307011
2216	100	-0.7788385162299498
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	354.0
3	42.0
4	55.0
5	140.0
6	461.0
7	1069.0
8	2248.0
9	3581.0
10	4872.0
11	6758.0
12	10053.0
13	14307.0
14	15446.0
15	18931.0
16	23692.0
17	33594.0
18	34186.0
19	18095.0
20	12932.0
21	12199.0
22	12478.0
23	13877.0
24	16018.0
25	19359.0
26	24463.0
27	31511.0
28	40940.0
29	53196.0
30	68978.0
31	89612.0
32	116031.0
33	153009.0
34	210769.0
35	308823.0
36	510268.0
37	986454.0
38	1421142.0
39	577845.0
40	1761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40814066410919	20.39416151747743	14.247889382440674	22.949808435972706
2	16.07066691391749	20.995311886172622	39.48788686590894	23.446134334000952
3	17.63832749192995	26.146404934009286	31.08211869312743	25.133148880933327
4	12.631375102704585	18.156383681527796	38.3261365682941	30.886104647473513
5	12.182846936979438	37.2798349032626	35.37579204186241	15.161526117895551
6	29.108546004046786	38.56523173959589	18.16332100430862	14.162901252048703
7	25.549019015929403	33.01053136543035	23.167812871376796	18.272636747263455
8	24.53009508683453	35.08263290912567	21.33885499457958	19.04841700946022
9	24.519827195495928	16.595931163235033	22.58964844588277	36.29459319538626
10-11	23.069990670593928	26.95057591575729	29.32879410393036	20.650639309718414
12-13	24.3362064946877	24.774645454846024	29.423494865746292	21.465653184719983
14-15	21.67616549294401	26.78982180896013	27.559153835396256	23.974858862699605
16-17	20.238999545953845	29.845433324523484	28.05006172029484	21.865505409227836
18-19	19.96779373202734	27.82418864662826	31.444965437250964	20.763052184093432
20-21	21.360530513195368	27.31100970541625	31.04813197279666	20.280327808591718
22-23	20.89436824642282	27.341330788539143	31.169303358483507	20.59499760655453
24-25	20.671349646548375	27.29279446618157	31.25345899589469	20.782396891375363
26-27	20.5189433354095	27.10133936428199	31.39734295722253	20.982374343085983
28-29	20.605234694219117	27.13301580906158	31.44631053101632	20.815438965702985
30-31	20.994264561256085	27.68167031484846	30.863073767201026	20.46099135669443
32-33	20.41500147138883	27.408883245655808	31.379661648337454	20.796453634617908
34-35	20.579749787916704	26.913868204221785	31.346742788705896	21.15963921915561
36-37	21.27794586315899	27.48180580994256	30.52709809471062	20.71315023218783
38-39	21.020334737364795	27.49690987810165	30.75180062876459	20.730954755768963
40-41	20.758339221969017	27.267935901250812	30.84072056775689	21.133004309023278
42-43	20.796196937334443	27.344975889964346	30.577071921855598	21.281755250845613
44-45	21.08480682707988	27.590583850783716	30.266745441929015	21.057863880207385
46-47	21.08230146159326	27.979921754560845	29.760928578806784	21.17684820503911
48-49	21.096666241575964	27.649470207610605	29.781371950461942	21.47249160035149
50-51	21.204571511653338	27.538135461826137	29.572451165395396	21.684841861125125
52-53	21.1510963335619	27.766442025739963	29.393913070799783	21.688548569898362
54-55	21.341401431631553	28.22613552096919	29.167208297934778	21.26525474946448
56-57	21.013568196972656	27.884676794503964	29.21929731069551	21.882457697827867
58-59	20.846437729654223	27.394220696824284	29.663301467959354	22.096040105562135
60-61	21.275964160130638	27.88857859321264	29.130931837835494	21.704525408821226
62-63	21.059650493300303	27.805788585349482	29.327387402816974	21.807173518533236
64-65	21.031013344356943	27.505575978391427	29.561259163836322	21.90215151341531
66-67	20.928375502536273	27.6318607739649	29.257955921585342	22.181807801913482
68-69	21.475551431970395	27.789555049143154	29.078411573638547	21.656481945247908
70-71	21.460693793203436	27.47131202499451	29.15844978662295	21.9095443951791
72-73	21.12058016050357	27.601703977103426	29.439369025755774	21.838346836637232
74-75	21.14967936455717	27.62305092319638	29.18632711160726	22.040942600639195
76-77	21.256927489588872	27.744386595144643	28.855649671047566	22.14303624421892
78-79	21.34392733290085	27.81551227844714	28.989152794232076	21.851407594419936
80-81	21.12278355627189	27.591515051574167	29.121690229293705	22.164011162860234
82-83	20.942785011950356	27.615106582897965	29.253984915760167	22.188123489391508
84-85	21.034979372739897	27.57069629144679	29.031464452296905	22.362859883516407
86-87	21.210899015252522	27.67928477586868	28.90872770379437	22.20108850508443
88-89	20.907370840182484	27.526894594014873	28.999966013073774	22.56576855272887
90-91	20.732594744834365	27.382376083360278	29.030856983967702	22.85417218783766
92-93	20.87377434546706	27.63996647733328	28.800342783847594	22.685916393352063
94-95	20.738472747868304	27.33804336408609	29.05082444704953	22.87265944099608
96-97	20.679873363405402	27.348409480227488	29.055280448840538	22.91643670752658
98-99	20.615176727771566	27.419106088002966	28.940095430840362	23.025621753385106
100	20.752119396756903	27.43754756603593	28.799235082695596	23.01109795451157
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1710.0
1	1243.5
2	2379.0
3	3485.5
4	1907.5
5	902.5
6	1715.5
7	4014.5
8	6486.5
9	7406.5
10	5694.5
11	3579.5
12	3257.0
13	3577.5
14	4040.0
15	4486.0
16	4989.0
17	5561.0
18	6273.0
19	7160.5
20	8225.0
21	9821.0
22	12323.5
23	16052.5
24	21095.5
25	27808.0
26	36664.5
27	47719.0
28	61782.0
29	76993.0
30	90536.5
31	103439.5
32	116623.5
33	128522.0
34	138571.0
35	147091.0
36	154693.0
37	161448.0
38	166985.5
39	169639.5
40	171878.0
41	172881.5
42	169729.5
43	167084.5
44	163763.0
45	160593.0
46	159243.0
47	156183.0
48	153634.0
49	152241.5
50	148671.5
51	141710.0
52	131560.5
53	123291.5
54	117944.5
55	112485.5
56	104599.5
57	94901.5
58	86604.5
59	78580.0
60	68493.5
61	57465.5
62	47112.0
63	37667.5
64	29661.0
65	22874.0
66	17371.0
67	12889.5
68	9674.5
69	7203.5
70	5055.5
71	3624.0
72	2634.5
73	1850.0
74	1266.0
75	806.0
76	479.0
77	333.5
78	222.0
79	110.5
80	54.5
81	33.0
82	14.5
83	6.0
84	4.0
85	2.5
86	1.0
87	0.5
88	1.0
89	0.5
90	1.0
91	1.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	1.0
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12654149285693603
2	0.0
3	0.0
4	0.0
5	0.013266115609474306
6	2.053578267720481E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.7455415275624087E-4
82-83	6.058055889775419E-4
84-85	4.5178721889850576E-4
86-87	5.441982409459275E-4
88-89	6.058055889775419E-4
90-91	7.290202850407708E-4
92-93	1.6428626141763846E-4
94-95	2.566972834650601E-4
96-97	5.031266755915177E-4
98-99	0.0011089322645690598
100	6.160734803161443E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4869549.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.98625808528935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7524665115926	52.62566838172346
2	13.203424301140299	17.424891270892502
3	3.4475043358097897	6.824637325586963
4	1.293461138840473	3.4140264212327893
5	0.6239873174163365	2.058729408449087
6	0.394626817077372	1.5623968199426241
7	0.24615640764920366	1.1370058171141633
8	0.1732572942897672	0.9146080428930805
9	0.12932276204416837	0.7680172637294059
>10	0.6790914044679274	7.960312788451594
>50	0.03865468035380381	1.7461153430418614
>100	0.01720577575530266	1.9880412180033127
>500	3.7389089796105636E-4	0.17397625203039108
>1k	3.427326210321422E-4	0.4429170919519455
>5k	6.231502200530051E-5	0.29377720497935067
>10k+	6.231502200530051E-5	0.6648793499774107
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16485	0.3385323774337213	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15854	0.32557429856440506	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9179	0.18849794919406296	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5110	0.10493784948051657	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.1071565354409616E-5	0.0	0.0	2.0535782677204808E-5	0.0
9	4.1071565354409616E-5	2.0535782677204808E-5	0.0	8.214313070881923E-5	0.0
10-11	5.1339456693012025E-5	2.0535782677204808E-5	0.0	8.214313070881923E-5	0.0
12-13	6.160734803161443E-5	2.0535782677204808E-5	0.0	8.214313070881923E-5	0.0
14-15	6.160734803161443E-5	4.1071565354409616E-5	0.0	9.241102204742165E-5	0.0
16-17	6.160734803161443E-5	6.160734803161443E-5	0.0	1.1294680472462645E-4	0.0
18-19	6.160734803161443E-5	1.0267891338602405E-4	0.0	1.4375047874043366E-4	0.0
20-21	6.160734803161443E-5	1.4375047874043366E-4	0.0	1.5401837007903606E-4	0.0
22-23	6.160734803161443E-5	1.4375047874043366E-4	0.0	4.1071565354409615E-4	0.0
24-25	6.160734803161443E-5	1.7455415275624087E-4	0.0	0.0010267891338602403	0.0
26-27	6.160734803161443E-5	1.8482204409484327E-4	0.0	0.0020125067023660714	0.0
28-29	6.160734803161443E-5	1.8482204409484327E-4	0.0	0.004415193275599034	0.0
30-31	6.160734803161443E-5	1.8482204409484327E-4	0.0	0.01250629165041773	0.0
32-33	6.160734803161443E-5	1.8482204409484327E-4	0.0	0.03023893999218408	0.0
34-35	6.160734803161443E-5	1.8482204409484327E-4	0.0	0.06298324547098715	0.0
36-37	6.160734803161443E-5	1.9508993543344567E-4	0.0	0.11042090345533026	0.0
38-39	6.160734803161443E-5	2.156257181106505E-4	0.0	0.17048806778615433	0.0
40-41	6.160734803161443E-5	2.258936094492529E-4	0.0	0.2375065945532122	0.0
42-43	6.160734803161443E-5	2.669651748036625E-4	0.0	0.30896085037854637	0.0
44-45	6.160734803161443E-5	2.7723306614226494E-4	0.0	0.39809641508895377	0.0
46-47	6.160734803161443E-5	2.875009574808673E-4	0.0	0.5027056920466351	0.0
48-49	6.160734803161443E-5	2.875009574808673E-4	0.0	0.6140507057224396	0.0
50-51	6.160734803161443E-5	3.080367401580721E-4	0.0	0.729995734717938	0.0
52-53	6.160734803161443E-5	3.2857252283527693E-4	0.0	0.8468032665858789	0.0
54-55	6.160734803161443E-5	3.4910830551248173E-4	0.0	0.9812099642081844	0.0
56-57	6.160734803161443E-5	3.4910830551248173E-4	0.0	1.1429189848998336	0.0
58-59	7.187523937021683E-5	3.4910830551248173E-4	0.0	1.3178222459615871	0.0
60-61	8.214313070881923E-5	3.6964408818968654E-4	0.0	1.4971612360816167	0.0
62-63	8.214313070881923E-5	3.6964408818968654E-4	0.0	1.681849797589058	0.0
64-65	8.214313070881923E-5	3.6964408818968654E-4	0.0	1.8852156534414173	0.0
66-67	8.214313070881923E-5	3.6964408818968654E-4	0.0	2.1279383367946396	0.0
68-69	8.214313070881923E-5	3.6964408818968654E-4	0.0	2.390816890845538	0.0
70-71	8.214313070881923E-5	3.6964408818968654E-4	0.0	2.671571843716944	0.0
72-73	8.214313070881923E-5	3.9017987086689134E-4	0.0	2.9737866894860283	0.0
74-75	8.214313070881923E-5	3.9017987086689134E-4	0.0	3.2761144820598376	0.0
76-77	8.214313070881923E-5	4.107156535440962E-4	0.0	3.605549507767557	0.0
78-79	8.214313070881923E-5	4.107156535440962E-4	0.0	3.972801177275349	0.0
80-81	8.214313070881923E-5	4.107156535440962E-4	0.0	4.367519456113903	0.0
82-83	8.214313070881923E-5	4.107156535440962E-4	0.0	4.776900283783981	0.0
84-85	8.214313070881923E-5	4.107156535440962E-4	0.0	5.190552554250917	0.0
86-87	8.214313070881923E-5	4.2098354488269863E-4	0.0	5.6375344000029575	0.0
88	8.214313070881923E-5	4.31251436221301E-4	0.0	5.990226199592611	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9115	0.0	57.82456	1
GTATCAA	16845	0.0	40.452793	1
ATCAACG	20725	0.0	32.635864	3
TCAACGC	21020	0.0	32.244926	4
TATCAAC	21665	0.0	31.328342	2
CAACGCA	21795	0.0	31.055214	5
AACGCAG	22910	0.0	29.684668	6
TATAACG	435	0.0	29.1745	2
GTGGTAT	3710	0.0	28.413525	1
TACCTGG	3785	0.0	27.94123	2
TGGTATC	3910	0.0	26.206474	2
ACGCAGA	25950	0.0	26.080378	7
CGCAGAG	26190	0.0	25.66193	8
GTACCTG	4510	0.0	24.103851	1
GCAGAGT	28545	0.0	23.330742	9
GTACATG	13245	0.0	23.094704	1
GTACACG	515	0.0	21.930826	1
TACATGG	13560	0.0	21.664528	2
ATAACGC	590	0.0	21.510012	3
TAACGCA	740	0.0	20.960957	4
>>END_MODULE