FastQCFastQC Report
Wed 25 May 2016
SRR2049468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5518234
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99270.17989450972901838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58650.041.290531
GTATCAA99500.035.7753641
TCAACGC105150.032.6734434
ATCAACG105750.032.400063
CAACGCA115300.029.9202615
TATCAAC122450.028.3267142
AACGCAG123950.027.9472626
ACGCAGA140050.024.4328677
CGCAGAG142500.023.4824858
GTACCTG42000.023.3995911
GTACATG163700.022.9801351
TACATGG163950.022.1598452
TACCTGG41600.022.144272
ACATGGG166950.020.804473
GTGGTAT35750.019.993041
GCAGAGT163250.019.7499319
CATGGGG116100.018.4594784
AGAGTAC143250.017.56982210-11
CAGAGTA154400.017.25990110-11
ACCTGGG54700.016.6691343
Produced by FastQC (version 0.11.5)