##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049468_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5518234
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.640030125580033	31.0	31.0	34.0	27.0	34.0
2	30.856730069801316	31.0	31.0	34.0	27.0	34.0
3	30.984178815178915	31.0	31.0	34.0	27.0	34.0
4	34.55828023965638	37.0	35.0	37.0	32.0	37.0
5	34.60874765368776	37.0	35.0	37.0	32.0	37.0
6	34.602380942888615	37.0	35.0	37.0	32.0	37.0
7	34.49261919664878	37.0	35.0	37.0	31.0	37.0
8	34.460737620042934	37.0	35.0	37.0	31.0	37.0
9	36.02966981827882	39.0	37.0	39.0	31.0	39.0
10-11	35.973571889122496	39.0	37.0	39.0	31.0	39.0
12-13	35.894650534935636	39.0	37.0	39.0	30.0	39.0
14-15	37.11132664906925	40.0	38.0	41.0	31.0	41.0
16-17	37.098877104522934	40.0	38.0	41.0	31.0	41.0
18-19	37.11181285534467	40.0	38.0	41.0	31.0	41.0
20-21	37.153878668429066	40.0	38.0	41.0	31.0	41.0
22-23	37.112380881274696	40.0	38.0	41.0	31.0	41.0
24-25	37.11151004107474	40.0	37.0	41.0	31.0	41.0
26-27	37.10374569110335	40.0	37.0	41.0	31.0	41.0
28-29	37.0775196376232	40.0	37.0	41.0	31.0	41.0
30-31	37.0192013423135	40.0	37.0	41.0	30.5	41.0
32-33	36.97210430003512	40.0	37.0	41.0	30.0	41.0
34-35	36.90413744687159	40.0	37.0	41.0	30.0	41.0
36-37	36.79632016329862	40.0	37.0	41.0	30.0	41.0
38-39	36.69803391809771	40.0	37.0	41.0	30.0	41.0
40-41	36.58029942188026	40.0	37.0	41.0	30.0	41.0
42-43	36.4846719077154	40.0	36.5	41.0	30.0	41.0
44-45	36.333487670149545	40.0	36.0	41.0	29.5	41.0
46-47	36.20939996382901	40.0	36.0	41.0	29.0	41.0
48-49	36.10549996611235	40.0	36.0	41.0	29.0	41.0
50-51	35.99059807902311	40.0	35.0	41.0	28.5	41.0
52-53	35.87896906872743	39.0	35.0	41.0	28.0	41.0
54-55	35.7323202495581	39.0	35.0	41.0	28.0	41.0
56-57	35.57065729724401	39.0	35.0	41.0	28.0	41.0
58-59	35.384328754452966	39.0	35.0	41.0	28.0	41.0
60-61	35.18885924373631	39.0	35.0	40.5	27.0	41.0
62-63	34.93469214969862	38.0	34.0	40.0	27.0	41.0
64-65	34.696633560664516	38.0	34.0	40.0	26.0	41.0
66-67	34.47082816712738	37.0	34.0	40.0	26.0	41.0
68-69	34.186139351828864	37.0	34.0	40.0	26.0	41.0
70-71	33.879213712213	36.0	34.0	39.0	26.0	41.0
72-73	33.54611411911854	36.0	33.5	39.0	26.0	41.0
74-75	33.24806722585523	35.5	33.0	39.0	25.0	40.0
76-77	32.92547371133591	35.0	33.0	37.5	25.0	40.0
78-79	32.58623320431863	35.0	33.0	37.0	25.0	39.0
80-81	32.25952388028489	35.0	33.0	37.0	24.0	39.0
82-83	31.970827079823003	35.0	33.0	36.0	24.0	39.0
84-85	31.693415502133472	35.0	32.5	36.0	23.5	37.0
86-87	31.428120663241174	35.0	32.0	35.5	22.5	37.0
88-89	31.209274380173078	35.0	32.0	35.0	21.5	36.5
90-91	31.009420586368755	35.0	32.0	35.0	20.0	36.0
92-93	30.810062693970572	35.0	32.0	35.0	19.5	36.0
94-95	30.613645144442952	35.0	32.0	35.0	18.0	36.0
96-97	30.427485858700447	35.0	32.0	35.0	11.0	35.5
98-99	30.258574482343448	35.0	32.0	35.0	2.0	35.0
100	30.129301512041714	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12617189086154212
1101	2	0.150706879535079
1101	3	0.25429735538720166
1101	4	0.3273046544307192
1101	5	0.27536430219782915
1101	6	0.31047398265931037
1101	7	0.3183808006865121
1101	8	0.27267772121727774
1101	9	0.4009721941548463
1101	10-11	0.44979807659031223
1101	12-13	0.4328692244359402
1101	14-15	0.532478586905512
1101	16-17	0.4712290898466094
1101	18-19	0.48468458042790985
1101	20-21	0.4868810995663324
1101	22-23	0.4553278870604487
1101	24-25	0.4531682152758023
1101	26-27	0.4839838383336641
1101	28-29	0.436627670909175
1101	30-31	0.4457814598461525
1101	32-33	0.45133219594479357
1101	34-35	0.42121494467046716
1101	36-37	0.3659950235532392
1101	38-39	0.42326761020747483
1101	40-41	0.44473025986766856
1101	42-43	0.4187569270832441
1101	44-45	0.3867884541682969
1101	46-47	0.37139936316950894
1101	48-49	0.32208804703009974
1101	50-51	0.328863531205954
1101	52-53	0.33875383549499816
1101	54-55	0.3062154044425398
1101	56-57	0.3244513790120891
1101	58-59	0.3377847238467311
1101	60-61	0.28998608092805256
1101	62-63	0.2601968835827435
1101	64-65	0.24230091155720856
1101	66-67	0.30214474054116636
1101	68-69	0.24966758222575436
1101	70-71	0.265673374001274
1101	72-73	0.23305865176558171
1101	74-75	0.21089703644788926
1101	76-77	0.22454134527897907
1101	78-79	0.25207987472887794
1101	80-81	0.28674484023346025
1101	82-83	0.2518066663746552
1101	84-85	0.2115732705072766
1101	86-87	0.2247357545649038
1101	88-89	0.2414982932040708
1101	90-91	0.2641897266049149
1101	92-93	0.29019353441022133
1101	94-95	0.3176460974727391
1101	96-97	0.3026157793302424
1101	98-99	0.33823270462809063
1101	100	0.3086549191822101
1102	1	0.1342557076230193
1102	2	0.2248799210861634
1102	3	0.23977856500499684
1102	4	0.2112373022241485
1102	5	0.21774270896988668
1102	6	0.25848084885964084
1102	7	0.2371839738738828
1102	8	0.32490279819768375
1102	9	0.34248030235974625
1102	10-11	0.3927816248263909
1102	12-13	0.3470582206080266
1102	14-15	0.4414269889278515
1102	16-17	0.3742929142997298
1102	18-19	0.4264825323355126
1102	20-21	0.44239652933060825
1102	22-23	0.38954841727233
1102	24-25	0.35793213207983143
1102	26-27	0.359873259422244
1102	28-29	0.3885888698848703
1102	30-31	0.3150277980005427
1102	32-33	0.3165984061722966
1102	34-35	0.327666205477783
1102	36-37	0.3796556668033162
1102	38-39	0.38094207283690906
1102	40-41	0.3646119262088803
1102	42-43	0.33573022206635983
1102	44-45	0.26693288143492566
1102	46-47	0.24186037582320097
1102	48-49	0.19537173144752273
1102	50-51	0.25537706705183183
1102	52-53	0.26072333155580196
1102	54-55	0.2288786408514838
1102	56-57	0.2676574477372
1102	58-59	0.30278398623330816
1102	60-61	0.2595668606835062
1102	62-63	0.27418169300986506
1102	64-65	0.21575838281373194
1102	66-67	0.2707277382532922
1102	68-69	0.2575434175836193
1102	70-71	0.18561613861049864
1102	72-73	0.21325667325614717
1102	74-75	0.23272446731049
1102	76-77	0.2168219862948888
1102	78-79	0.19209723635814413
1102	80-81	0.19383070440514416
1102	82-83	0.2141207600779964
1102	84-85	0.21623626644571203
1102	86-87	0.2008653477007556
1102	88-89	0.15286871072737895
1102	90-91	0.17307276669782823
1102	92-93	0.13838096667129918
1102	94-95	0.17299980655971225
1102	96-97	0.1519207598836836
1102	98-99	0.1427147934128712
1102	100	0.07564172997200203
1103	1	0.20783996299197938
1103	2	0.28859914640085194
1103	3	0.3515559989893937
1103	4	0.3386935362074013
1103	5	0.2592457939331254
1103	6	0.324123857819437
1103	7	0.3601758518572282
1103	8	0.36858695441220846
1103	9	0.45582732126189995
1103	10-11	0.43656236586200947
1103	12-13	0.45975737071870526
1103	14-15	0.5211111794038814
1103	16-17	0.4657698748635042
1103	18-19	0.42134257203807124
1103	20-21	0.4522454891063816
1103	22-23	0.4999914471153275
1103	24-25	0.5031679674957061
1103	26-27	0.4580566245630351
1103	28-29	0.49492871711933617
1103	30-31	0.5371670131101993
1103	32-33	0.4859741247972167
1103	34-35	0.4774409564598443
1103	36-37	0.46583698114815775
1103	38-39	0.5238223072508745
1103	40-41	0.532123084651623
1103	42-43	0.4755518419749123
1103	44-45	0.4605645237317333
1103	46-47	0.3847490940390301
1103	48-49	0.37099740163728256
1103	50-51	0.37829148955577097
1103	52-53	0.38486831320346226
1103	54-55	0.3352093924714552
1103	56-57	0.2965297832421925
1103	58-59	0.33711670870571453
1103	60-61	0.30322352611702996
1103	62-63	0.3965060834099532
1103	64-65	0.341780201683747
1103	66-67	0.2993712966208264
1103	68-69	0.3100145899920079
1103	70-71	0.27826374146741273
1103	72-73	0.3921302527801558
1103	74-75	0.4043565917156471
1103	76-77	0.32917293344203813
1103	78-79	0.3631786886055508
1103	80-81	0.32752795748877617
1103	82-83	0.3104422570122054
1103	84-85	0.3003427202124591
1103	86-87	0.310914043907637
1103	88-89	0.3608540484336764
1103	90-91	0.3393997821819923
1103	92-93	0.30590101679099035
1103	94-95	0.3489903210166396
1103	96-97	0.32827819877073594
1103	98-99	0.3127199856627563
1103	100	0.2966975472700675
1104	1	0.17659582286551512
1104	2	0.1563036165185956
1104	3	0.18789746700573318
1104	4	0.2567760865740496
1104	5	0.27126395114059676
1104	6	0.21442571546853628
1104	7	0.2546158463624195
1104	8	0.23054267127091777
1104	9	0.30771680254624556
1104	10-11	0.2598237775393599
1104	12-13	0.2871119347581512
1104	14-15	0.36736641908392187
1104	16-17	0.35772487758362814
1104	18-19	0.2813427887744524
1104	20-21	0.3447534658628797
1104	22-23	0.33317780251528006
1104	24-25	0.33580031975126445
1104	26-27	0.33991164944016106
1104	28-29	0.281602392115488
1104	30-31	0.3185901556599049
1104	32-33	0.28660164679401845
1104	34-35	0.31680118807775415
1104	36-37	0.3342030897524211
1104	38-39	0.3577687056925001
1104	40-41	0.3769092726500105
1104	42-43	0.4132838376133634
1104	44-45	0.37114363711152265
1104	46-47	0.3415305182028945
1104	48-49	0.27579047998155914
1104	50-51	0.30062661826678294
1104	52-53	0.2750746976959846
1104	54-55	0.29102372797720477
1104	56-57	0.2721345284280332
1104	58-59	0.292566521680925
1104	60-61	0.3194840485515087
1104	62-63	0.31966212846055697
1104	64-65	0.3537776208056016
1104	66-67	0.28883507833391775
1104	68-69	0.26171742850578994
1104	70-71	0.28666707883350284
1104	72-73	0.30675845398824464
1104	74-75	0.29886987898132844
1104	76-77	0.20323423491127102
1104	78-79	0.20273514461329256
1104	80-81	0.21395325882641458
1104	82-83	0.2091605965433061
1104	84-85	0.18614093614457872
1104	86-87	0.19874832664508446
1104	88-89	0.14467537906721262
1104	90-91	0.13452372810641933
1104	92-93	0.08134427777854825
1104	94-95	0.13616657494186768
1104	96-97	0.19425946651017867
1104	98-99	0.18666601923880677
1104	100	0.18795388700419124
1105	1	0.1562899348579343
1105	2	0.12222760001698063
1105	3	0.17558694789687834
1105	4	0.15769061335225132
1105	5	0.2073473042988141
1105	6	0.24292829101725744
1105	7	0.25131795284521274
1105	8	0.23855653464428173
1105	9	0.30227659451627886
1105	10-11	0.34246216445280453
1105	12-13	0.34100450061248466
1105	14-15	0.3535274475209107
1105	16-17	0.3038342126765201
1105	18-19	0.35544871724873417
1105	20-21	0.34143458619120537
1105	22-23	0.3173481302346488
1105	24-25	0.33201244383214146
1105	26-27	0.3157401814554035
1105	28-29	0.28927408498761054
1105	30-31	0.32730663487903655
1105	32-33	0.330209239907326
1105	34-35	0.32084131439274444
1105	36-37	0.3242686940920194
1105	38-39	0.3066362167594221
1105	40-41	0.42606207272710606
1105	42-43	0.3367896908192165
1105	44-45	0.30061241339439704
1105	46-47	0.26105148616582596
1105	48-49	0.2907408695000413
1105	50-51	0.30720296413968384
1105	52-53	0.2634579713113041
1105	54-55	0.21232901892072675
1105	56-57	0.20943341237661173
1105	58-59	0.1974287705623965
1105	60-61	0.301542567795714
1105	62-63	0.29707621708178067
1105	64-65	0.27841303048730026
1105	66-67	0.2812433085538615
1105	68-69	0.2803674778743641
1105	70-71	0.22835574221131338
1105	72-73	0.2536716852746892
1105	74-75	0.2542657729777247
1105	76-77	0.21318777636687258
1105	78-79	0.2681588942270068
1105	80-81	0.2506507497184529
1105	82-83	0.195586606421152
1105	84-85	0.19090245299928554
1105	86-87	0.15126744373673873
1105	88-89	0.17413983391386978
1105	90-91	0.20153953995826868
1105	92-93	0.13536328313085733
1105	94-95	0.1503397496894472
1105	96-97	0.12225654610994496
1105	98-99	0.16001477695389
1105	100	0.10394092590671988
1106	1	0.19938121617585836
1106	2	0.16517310881526015
1106	3	0.17553832270677816
1106	4	0.17658002019338426
1106	5	0.22896038425412968
1106	6	0.2131726778661971
1106	7	0.2731845668677124
1106	8	0.2867445838444951
1106	9	0.3081504968703328
1106	10-11	0.31401741458582677
1106	12-13	0.27459183174098456
1106	14-15	0.31868016013448397
1106	16-17	0.3457516439238759
1106	18-19	0.33765111743110765
1106	20-21	0.35720091764143547
1106	22-23	0.38047733272337325
1106	24-25	0.36214774714077436
1106	26-27	0.33366649154764616
1106	28-29	0.3097152139488202
1106	30-31	0.28163626052321433
1106	32-33	0.32775764261841545
1106	34-35	0.2829189143369746
1106	36-37	0.3611060295817623
1106	38-39	0.28464128258266186
1106	40-41	0.3646458003948254
1106	42-43	0.30341956840018725
1106	44-45	0.25707654944599767
1106	46-47	0.2654895763102729
1106	48-49	0.25083179256969856
1106	50-51	0.25998114001198047
1106	52-53	0.2634590827862766
1106	54-55	0.22310555643474572
1106	56-57	0.16379770720720188
1106	58-59	0.24473403628182666
1106	60-61	0.19741876008264825
1106	62-63	0.25818333743339394
1106	64-65	0.16406801790739678
1106	66-67	0.2235919258458665
1106	68-69	0.18191030083384874
1106	70-71	0.17093832866658687
1106	72-73	0.15317431126313608
1106	74-75	0.18098814147054298
1106	76-77	0.08639573090621866
1106	78-79	0.08438041516495076
1106	80-81	0.08405921434385277
1106	82-83	0.12620038866671024
1106	84-85	0.14303285978219904
1106	86-87	0.16337194358366958
1106	88-89	0.12417614086969309
1106	90-91	0.08144047294214118
1106	92-93	-0.01962892881549294
1106	94-95	0.057664148972186524
1106	96-97	0.08014249420288522
1106	98-99	0.15447410363118053
1106	100	0.19703265255071045
1107	1	0.05089761711388974
1107	2	0.09965008711652246
1107	3	0.12551078129455817
1107	4	0.07921782735588323
1107	5	0.11447619865206349
1107	6	0.16149633112360107
1107	7	0.12046835596001415
1107	8	0.09319955498823873
1107	9	0.08253492736911738
1107	10-11	0.13164662384969716
1107	12-13	0.1250901202775836
1107	14-15	0.18822628415416176
1107	16-17	0.19820888980429885
1107	18-19	0.1987853691000936
1107	20-21	0.18353429534403887
1107	22-23	0.14245005536241706
1107	24-25	0.14241002363058897
1107	26-27	0.25677649595210283
1107	28-29	0.20897511289510362
1107	30-31	0.17232347557241212
1107	32-33	0.15030253403816118
1107	34-35	0.11277586132003137
1107	36-37	0.1510845267188614
1107	38-39	0.15048030005224433
1107	40-41	0.23370029126828484
1107	42-43	0.15989905085972111
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2216	92-93	-1.1704629930185853
2216	94-95	-1.1179189417248558
2216	96-97	-1.091494117977362
2216	98-99	-1.1241729364553912
2216	100	-1.1773900850100105
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107442.0
3	19603.0
4	12563.0
5	10022.0
6	8497.0
7	8337.0
8	8152.0
9	9352.0
10	10420.0
11	11613.0
12	12598.0
13	14258.0
14	15408.0
15	16223.0
16	16965.0
17	18494.0
18	17807.0
19	15806.0
20	15829.0
21	17316.0
22	20427.0
23	26669.0
24	30566.0
25	36441.0
26	44736.0
27	49796.0
28	59365.0
29	74531.0
30	90817.0
31	105924.0
32	134291.0
33	179022.0
34	248451.0
35	366277.0
36	607093.0
37	1084262.0
38	1456216.0
39	535521.0
40	1124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76284421614232	20.293760850905006	14.129766713136757	22.813628219815918
2	15.362807979580728	20.890459558646953	40.20173508703981	23.544997374732514
3	17.993678453727817	26.33653180592538	30.43442641818375	25.235363322163057
4	12.616195047830042	17.782876152979288	38.74104647946902	30.85988231972165
5	12.972155171023298	38.096422151247324	35.16526733598453	13.766155341744843
6	29.735523743020558	38.36602957223435	18.287451309948818	13.610995374796273
7	26.182345203745367	33.08978781814454	22.982316638049618	17.745550340060472
8	24.38002756580861	36.40625948650752	21.017687607438237	18.19602534024563
9	24.649234965497556	17.811471213226465	21.847355567738518	35.691938253537465
10-11	23.29622852569377	27.67471423776317	29.128717881426525	19.900339355116532
12-13	23.767124380100434	25.455203205262745	28.985690066346155	21.79198234829067
14-15	21.368901622083822	27.612451341699096	26.211302508759786	24.807344527457296
16-17	20.99049018574591	30.735383285632174	26.840719197543894	21.433407331078026
18-19	20.970943763376678	29.76058877456826	28.646396806823343	20.622070655231724
20-21	22.027066777416525	29.031675172572406	28.152777896087365	20.788480153923697
22-23	21.780429418654006	28.915392208001222	28.189837944897565	21.1143404284472
24-25	21.564523107021145	28.936514619611703	28.1641781225005	21.334784150866657
26-27	21.328161833019223	28.97683906903933	28.209359861013212	21.48563923692824
28-29	21.389434895335164	28.949531122155992	28.357497120345236	21.303536862163604
30-31	21.613142046247447	29.229481070951536	27.974150508091217	21.1832263747098
32-33	21.316490108834536	29.082862864858498	28.273101285896818	21.327545740410145
34-35	21.55300511568049	28.79860616872013	28.245988711684728	21.402400003914646
36-37	21.741891122131427	28.992827973025655	27.95600765320686	21.309273251636057
38-39	21.56086059833485	29.098187537196434	28.033872127904903	21.30707973656381
40-41	21.524746252004086	28.83375061305139	28.19822520490325	21.443277930041276
42-43	21.409639767163544	28.905545954771473	28.152878916759704	21.53193536130528
44-45	21.576010682348223	29.000719394599265	28.016006122013604	21.40726380103891
46-47	21.60201455544767	29.003069828122612	27.896521580418636	21.498394036011074
48-49	21.524372903493123	28.92250127011591	27.983240813794936	21.569885012596032
50-51	21.524999574094352	28.931181621754465	27.942328388115673	21.60149041603551
52-53	21.5711755802444	28.92905581160544	27.862560037508405	21.637208570641754
54-55	21.607413517152484	29.179722622003744	27.77519815238409	21.43766570845968
56-57	21.534071676470308	28.99651219643716	27.79305910324886	21.67635702384367
58-59	21.50043493231366	28.721480219640817	28.05722984360559	21.720855004439933
60-61	21.693839516790447	28.99895240079274	27.77000553385378	21.537202548563034
62-63	21.507659340740005	29.069998106902812	27.790875199040276	21.631467353316914
64-65	21.618092872440805	28.81357130382366	27.882577483350317	21.68575834038522
66-67	21.466208850211917	28.856482878552676	27.87729757797696	21.800010693258447
68-69	21.675109427845136	28.990556051406735	27.788850226694457	21.545484294053672
70-71	21.70957307303184	28.76689136984105	27.821657115444935	21.701878441682172
72-73	21.51471375891204	28.87409452222765	27.993752169385626	21.61743954947468
74-75	21.522082904476086	28.952909820395174	27.826844051418824	21.698163223709916
76-77	21.671933346237243	28.897732536770704	27.680342997490985	21.74999111950107
78-79	21.657266632008014	28.880533034025625	27.89908659011859	21.56311374384778
80-81	21.580900145184707	28.829341811201882	27.899026845576756	21.69073119803665
82-83	21.442486771297688	28.907614396829896	28.000254823338196	21.649644008534224
84-85	21.52160152747364	28.808781704751564	27.86822911923329	21.80138764854151
86-87	21.618050320159302	28.98479092655571	27.7629683842633	21.63419036902169
88-89	21.450229523765938	28.872000909848428	27.887987146125376	21.78978242026026
90-91	21.427628298022988	28.823439268147265	27.851458116724555	21.897474317105193
92-93	21.459196432770515	28.948426483206525	27.835452405843757	21.756924678179203
94-95	21.404280654948543	28.782782770082903	27.960281872917943	21.85265470205061
96-97	21.47166739450188	28.86986841059548	27.868056631046993	21.790407563855645
98-99	21.38586776517253	28.935830145170705	27.830654805514605	21.84764728414216
100	21.502531609323512	28.83694942616976	27.794167059030773	21.866351905475952
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8936.0
1	6672.0
2	5698.0
3	6597.0
4	5267.5
5	3801.5
6	2927.5
7	2512.5
8	2472.5
9	2451.5
10	2051.5
11	1658.5
12	1616.0
13	1663.0
14	1716.5
15	1840.5
16	2057.0
17	2430.5
18	3079.5
19	3885.5
20	5061.5
21	7070.5
22	10313.0
23	15442.5
24	22191.5
25	30449.5
26	41674.0
27	55058.0
28	70239.0
29	86970.0
30	102555.0
31	117126.5
32	131144.0
33	143158.5
34	153094.5
35	163026.5
36	171913.0
37	180595.5
38	189382.0
39	193728.5
40	197545.5
41	199032.5
42	196941.0
43	195709.5
44	192493.0
45	188642.0
46	184638.0
47	178630.0
48	173448.5
49	169906.5
50	165399.0
51	157606.0
52	147356.5
53	138834.0
54	132692.0
55	126332.0
56	116055.0
57	104167.5
58	94487.5
59	85888.0
60	75915.5
61	64988.5
62	55459.5
63	46241.5
64	37794.5
65	31187.0
66	25516.0
67	20129.0
68	15536.5
69	11916.0
70	8884.0
71	6549.0
72	4911.0
73	3574.5
74	2612.0
75	1899.5
76	1289.5
77	864.0
78	568.0
79	387.5
80	262.5
81	172.5
82	126.0
83	101.0
84	88.5
85	72.5
86	49.5
87	39.0
88	33.5
89	30.5
90	25.5
91	21.5
92	15.0
93	11.0
94	12.5
95	11.0
96	13.0
97	14.0
98	10.5
99	11.0
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07779662841409045
2	0.012703339510430331
3	0.0165451483210027
4	0.010365635092676389
5	0.008644069823787827
6	0.007665495881472224
7	0.020423200610920088
8	0.009586400286758409
9	0.009223965493308185
10-11	0.01286643516748293
12-13	0.014642365655389024
14-15	0.009921652470699866
16-17	0.013111078653061832
18-19	0.008490035036571482
20-21	0.012594609072395261
22-23	0.013192626481588131
24-25	0.016037739610172384
26-27	0.0054274610319170954
28-29	0.01169758295860596
30-31	0.009495791588395853
32-33	0.012395269935997641
34-35	0.008934017658548007
36-37	0.00991259160086361
38-39	0.01943556579876823
40-41	0.013627548233728397
42-43	0.008254452420828838
44-45	0.01984330494139973
46-47	0.017886157056768524
48-49	0.018502296205633904
50-51	0.010510609010056477
52-53	0.009740435073974754
54-55	0.011543548171389615
56-57	0.020867183232896613
58-59	0.0024283131161164967
60-61	0.0068772002057179895
62-63	0.014868887401295414
64-65	0.025143913795609246
66-67	0.013391965617985755
68-69	0.014234626512757522
70-71	0.025379496411351895
72-73	0.02027822669354
74-75	0.02083093975355159
76-77	0.009532035067740875
78-79	0.011099565549413092
80-81	0.020368835391902554
82-83	0.012431513415342663
84-85	0.011353269904828248
86-87	0.01635487005444133
88-89	0.015068226537693038
90-91	0.016209896137061242
92-93	0.008163843722466282
94-95	0.01547596568032454
96-97	0.013237930830769408
98-99	0.010102869867424978
100	0.007248695869004468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5518234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.77898300712191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28297324214513	45.00339578167807
2	14.603197112517302	17.45928544077647
3	4.600615375127881	8.250603249962204
4	1.902437652825166	4.54903152481378
5	1.00849387675481	3.014336916065614
6	0.6105721242479598	2.1899628384024674
7	0.3841781898270919	1.6076047036966408
8	0.27008519204132625	1.2916334484410958
9	0.21146265455568952	1.137692018999291
>10	1.0547148030034563	11.020211064720156
>50	0.0514044182260155	2.084627502571162
>100	0.01919828900427883	1.897747435714447
>500	5.15465885182647E-4	0.1929494097972311
>1k	1.2128307095701622E-4	0.1209873560181349
>5k	3.0320767739248275E-5	0.17993130834334467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9927	0.17989450972901838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.812173967251117E-5	0.0
3	0.0	0.0	0.0	3.624347934502234E-5	0.0
4	0.0	0.0	0.0	3.624347934502234E-5	0.0
5	0.0	1.812173967251117E-5	0.0	3.624347934502234E-5	0.0
6	0.0	1.812173967251117E-5	0.0	3.624347934502234E-5	0.0
7	0.0	1.812173967251117E-5	0.0	3.624347934502234E-5	0.0
8	0.0	1.812173967251117E-5	0.0	3.624347934502234E-5	0.0
9	0.0	1.812173967251117E-5	0.0	5.4365219017533505E-5	0.0
10-11	1.812173967251117E-5	3.624347934502234E-5	0.0	8.154782852630026E-5	0.0
12-13	3.624347934502234E-5	7.248695869004468E-5	0.0	9.060869836255585E-5	0.0
14-15	3.624347934502234E-5	1.0873043803506702E-4	0.0	1.177913078713226E-4	0.0
16-17	3.624347934502234E-5	1.268521777075782E-4	0.0	1.4497391738008936E-4	0.0
18-19	3.624347934502234E-5	1.3591304754383376E-4	0.0	1.4497391738008936E-4	0.0
20-21	3.624347934502234E-5	1.4497391738008936E-4	0.0	1.812173967251117E-4	0.0
22-23	3.624347934502234E-5	1.4497391738008936E-4	0.0	2.446434855789008E-4	0.0
24-25	3.624347934502234E-5	1.4497391738008936E-4	0.0	4.3492175214026804E-4	0.0
26-27	3.624347934502234E-5	1.4497391738008936E-4	0.0	8.336000249355138E-4	0.0
28-29	3.624347934502234E-5	1.721565268888561E-4	0.0	0.0016400174403622609	0.0
30-31	5.4365219017533505E-5	2.0840000623387845E-4	0.0	0.0036243479345022343	0.0
32-33	5.4365219017533505E-5	2.1746087607013402E-4	0.0	0.007701739360817247	0.0
34-35	5.4365219017533505E-5	2.1746087607013402E-4	0.0	0.013663791713073421	0.0
36-37	5.4365219017533505E-5	2.6276522525141196E-4	0.0	0.023422348526720687	0.0
38-39	5.4365219017533505E-5	3.2619131410520103E-4	0.0	0.03546424453910436	0.0
40-41	5.4365219017533505E-5	3.3525218394145665E-4	0.0	0.048040731871827114	0.0
42-43	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.060744071382257445	0.0
44-45	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.07651904576717841	0.0
46-47	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.09600897678496417	0.0
48-49	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.11541735997422364	0.0
50-51	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.13545094318218476	0.0
52-53	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.15599193510097614	0.0
54-55	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.1786259879519426	0.0
56-57	5.4365219017533505E-5	3.8055653312273454E-4	0.0	0.20686871923155126	0.0
58-59	5.4365219017533505E-5	3.8961740295899016E-4	0.0	0.23628936358987312	0.0
60-61	5.4365219017533505E-5	4.3492175214026804E-4	0.0	0.2681745645436565	0.0
62-63	5.4365219017533505E-5	4.3492175214026804E-4	0.0	0.29990573071022364	0.0
64-65	5.4365219017533505E-5	4.3492175214026804E-4	0.0	0.3359861143981933	0.0
66-67	5.4365219017533505E-5	4.892869711578015E-4	0.0	0.37853595914924953	0.0
68-69	5.4365219017533505E-5	5.074087108303128E-4	0.0	0.4265948127607492	9.060869836255585E-6
70-71	5.4365219017533505E-5	5.074087108303128E-4	0.0	0.47912973607135906	1.812173967251117E-5
72-73	5.4365219017533505E-5	5.074087108303128E-4	0.0	0.534926572523021	1.812173967251117E-5
74-75	5.4365219017533505E-5	5.164695806665683E-4	0.0	0.5929976873035829	1.812173967251117E-5
76-77	5.4365219017533505E-5	5.255304505028239E-4	0.0	0.6590695501495587	1.812173967251117E-5
78-79	5.4365219017533505E-5	5.255304505028239E-4	0.0	0.7363678307226551	1.812173967251117E-5
80-81	8.154782852630026E-5	5.255304505028239E-4	0.0	0.8206157984601596	1.812173967251117E-5
82-83	9.060869836255585E-5	5.255304505028239E-4	0.0	0.9088233663161076	1.812173967251117E-5
84-85	9.060869836255585E-5	5.255304505028239E-4	0.0	1.0029023778259494	1.812173967251117E-5
86-87	9.060869836255585E-5	5.255304505028239E-4	0.0	1.102010172094913	2.7182609508766753E-5
88	9.060869836255585E-5	5.255304505028239E-4	0.0	1.1873907485619493	3.624347934502234E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5865	0.0	41.29053	1
GTATCAA	9950	0.0	35.775364	1
TCAACGC	10515	0.0	32.673443	4
ATCAACG	10575	0.0	32.40006	3
CAACGCA	11530	0.0	29.920261	5
TATCAAC	12245	0.0	28.326714	2
AACGCAG	12395	0.0	27.947262	6
ACGCAGA	14005	0.0	24.432867	7
CGCAGAG	14250	0.0	23.482485	8
GTACCTG	4200	0.0	23.399591	1
GTACATG	16370	0.0	22.980135	1
TACATGG	16395	0.0	22.159845	2
TACCTGG	4160	0.0	22.14427	2
ACATGGG	16695	0.0	20.80447	3
GTGGTAT	3575	0.0	19.99304	1
GCAGAGT	16325	0.0	19.749931	9
CATGGGG	11610	0.0	18.459478	4
AGAGTAC	14325	0.0	17.569822	10-11
CAGAGTA	15440	0.0	17.259901	10-11
ACCTGGG	5470	0.0	16.669134	3
>>END_MODULE