FastQCFastQC Report
Wed 25 May 2016
SRR2049468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5518234
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238200.43165983899921606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220390.39938502064247366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141800.2569662685562084No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101730.18435245768845612No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59410.10766125539438887No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.10005012473193417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108450.057.370261
GTATCAA199850.040.811191
ATCAACG245150.033.094083
TCAACGC248650.032.760584
TATCAAC256550.031.6968082
CAACGCA257850.031.5734625
GTGGTAT45300.030.544571
AACGCAG271450.030.1265686
ACGCAGA305950.026.5908977
TGGTATC49800.026.333972
CGCAGAG309750.026.2191588
ATAACGC5250.025.0691833
GCAGAGT335050.023.9587759
GTACCTG40800.023.0703771
GTACATG157800.022.9054641
TATAACG5950.022.1198672
TACCTGG41450.021.8863952
GAGTACT250750.021.62036312-13
CAGAGTA324450.021.6126210-11
TACATGG160200.021.5364912
Produced by FastQC (version 0.11.5)