##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049468_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5518234
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875947812289223	33.0	31.0	34.0	30.0	34.0
2	31.96060732473469	33.0	31.0	34.0	30.0	34.0
3	32.0087984670458	33.0	31.0	34.0	30.0	34.0
4	35.75264332755733	37.0	35.0	37.0	33.0	37.0
5	35.82434525248476	37.0	35.0	37.0	35.0	37.0
6	35.91579353104635	37.0	35.0	37.0	35.0	37.0
7	35.91469317901343	37.0	35.0	37.0	35.0	37.0
8	35.93907090565568	37.0	35.0	37.0	35.0	37.0
9	37.653132868232845	39.0	37.0	39.0	35.0	39.0
10-11	37.63807750813032	39.0	37.0	39.0	35.0	39.0
12-13	37.62297892767867	39.0	37.0	39.0	35.0	39.0
14-15	38.93134098698968	40.0	38.0	41.0	35.5	41.0
16-17	38.946563973184176	40.0	38.0	41.0	35.5	41.0
18-19	39.02091402792995	40.0	38.5	41.0	36.0	41.0
20-21	39.03725847798408	40.0	39.0	41.0	35.5	41.0
22-23	38.99694050669109	40.0	39.0	41.0	35.0	41.0
24-25	38.96919240829584	40.0	38.5	41.0	35.0	41.0
26-27	38.868134805446815	40.0	38.0	41.0	35.0	41.0
28-29	38.78314284968705	40.0	38.0	41.0	35.0	41.0
30-31	38.66114684516822	40.0	38.0	41.0	35.0	41.0
32-33	38.51489915070655	40.0	38.0	41.0	34.5	41.0
34-35	38.38893221998197	40.0	38.0	41.0	34.0	41.0
36-37	38.25484113214482	40.0	38.0	41.0	34.0	41.0
38-39	38.154375022878696	40.0	38.0	41.0	34.0	41.0
40-41	38.063658771991186	40.0	38.0	41.0	34.0	41.0
42-43	37.91858735965165	40.0	38.0	41.0	33.0	41.0
44-45	37.741320683392544	40.0	37.5	41.0	33.0	41.0
46-47	37.48692090621746	40.0	37.0	41.0	33.0	41.0
48-49	37.2336039029878	40.0	37.0	41.0	32.0	41.0
50-51	37.07484849319547	40.0	37.0	41.0	32.0	41.0
52-53	36.965028123127794	40.0	36.5	41.0	32.0	41.0
54-55	36.82489171354459	40.0	36.0	41.0	31.0	41.0
56-57	36.677290687564174	39.5	36.0	41.0	31.0	41.0
58-59	36.499128706756544	39.0	35.0	41.0	31.0	41.0
60-61	36.313045622929366	39.0	35.0	41.0	31.0	41.0
62-63	36.09459430317743	39.0	35.0	41.0	31.0	41.0
64-65	35.873974354838886	38.5	35.0	40.5	30.0	41.0
66-67	35.61290858633396	38.0	35.0	40.0	30.0	41.0
68-69	35.31995426435341	37.0	35.0	40.0	30.0	41.0
70-71	35.0375285825139	37.0	35.0	39.5	29.0	41.0
72-73	34.70418289619469	36.0	34.5	39.0	29.0	41.0
74-75	34.381490527585456	36.0	34.0	39.0	29.0	41.0
76-77	34.060767176600336	35.5	34.0	38.5	29.0	40.0
78-79	33.75397826551031	35.0	34.0	37.0	29.0	39.0
80-81	33.450766676440324	35.0	34.0	37.0	29.0	39.0
82-83	33.17803848115176	35.0	34.0	36.5	29.0	39.0
84-85	32.89979420952428	35.0	34.0	36.0	28.5	37.0
86-87	32.655986589187776	35.0	34.0	36.0	28.0	37.0
88-89	32.44068138466038	35.0	34.0	35.5	27.0	37.0
90-91	32.254193823603714	35.0	33.5	35.0	27.0	36.0
92-93	32.0945461899586	35.0	33.0	35.0	27.0	36.0
94-95	31.937162324033377	35.0	33.0	35.0	27.0	36.0
96-97	31.795394051792655	35.0	33.0	35.0	26.5	36.0
98-99	31.66462232301131	35.0	33.0	35.0	26.0	35.5
100	31.56135096844389	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23718430624519016
1101	2	0.2506216448083052
1101	3	0.3729314855498984
1101	4	0.23330519237111247
1101	5	0.2373534882442172
1101	6	0.15489111855242754
1101	7	0.2197924945255636
1101	8	0.2008875505990133
1101	9	0.2835739144396072
1101	10-11	0.29801279416921034
1101	12-13	0.24759833490492866
1101	14-15	0.39217556032759404
1101	16-17	0.3550266948246801
1101	18-19	0.35203850297384776
1101	20-21	0.3597787704962272
1101	22-23	0.3824068023706957
1101	24-25	0.3997596517454767
1101	26-27	0.3502970396319114
1101	28-29	0.3990030723486271
1101	30-31	0.3568369862080445
1101	32-33	0.35380671468297464
1101	34-35	0.3674806935315402
1101	36-37	0.3934846578288713
1101	38-39	0.3995348075684788
1101	40-41	0.4152390437287039
1101	42-43	0.46331461969679566
1101	44-45	0.43060227257639383
1101	46-47	0.3650190827481765
1101	48-49	0.32620209592449356
1101	50-51	0.2894607123998014
1101	52-53	0.3162802359747232
1101	54-55	0.3195160928691507
1101	56-57	0.35581788721410845
1101	58-59	0.33844778148407784
1101	60-61	0.20359423959668987
1101	62-63	0.29752159169843395
1101	64-65	0.32452601370337675
1101	66-67	0.34920413603630607
1101	68-69	0.3108017180697118
1101	70-71	0.31656006967475037
1101	72-73	0.3370479070753163
1101	74-75	0.26946460536830585
1101	76-77	0.2631952752011202
1101	78-79	0.2738358048185887
1101	80-81	0.2632954029723109
1101	82-83	0.20818177263313942
1101	84-85	0.21890109517467948
1101	86-87	0.15326449410888188
1101	88-89	0.19149904849273724
1101	90-91	0.23276126155731447
1101	92-93	0.21934487417355797
1101	94-95	0.23436983062037342
1101	96-97	0.19567606951375538
1101	98-99	0.27354211205751255
1101	100	0.3032979999436307
1102	1	0.16126000840627341
1102	2	0.17931446591104105
1102	3	0.2445441131506243
1102	4	0.1482369609975578
1102	5	0.21351621002922627
1102	6	0.19270715179534648
1102	7	0.23176272393612862
1102	8	0.1845174784322765
1102	9	0.24101302295916582
1102	10-11	0.24584936973280236
1102	12-13	0.24918224248239085
1102	14-15	0.28280573801142594
1102	16-17	0.3150983584456739
1102	18-19	0.3290443638028222
1102	20-21	0.32385264781022727
1102	22-23	0.2454007368859692
1102	24-25	0.22668023934622994
1102	26-27	0.24665391150291782
1102	28-29	0.2629915485245675
1102	30-31	0.28419404482742294
1102	32-33	0.3498747234934072
1102	34-35	0.3108823790421624
1102	36-37	0.3484468348884917
1102	38-39	0.27682923300173456
1102	40-41	0.28234084546674154
1102	42-43	0.26301867895827513
1102	44-45	0.2926341545309583
1102	46-47	0.3036105054552465
1102	48-49	0.2601254489360372
1102	50-51	0.26944508181706794
1102	52-53	0.2332189100271833
1102	54-55	0.29977589481355693
1102	56-57	0.2646790973605988
1102	58-59	0.26624557478347555
1102	60-61	0.270062120156183
1102	62-63	0.18122310640679729
1102	64-65	0.1968195053947781
1102	66-67	0.20712077463387146
1102	68-69	0.21452658058795038
1102	70-71	0.22309268114179304
1102	72-73	0.2285973727959032
1102	74-75	0.25190403366246983
1102	76-77	0.2186218076249844
1102	78-79	0.23495193757536015
1102	80-81	0.20542195526483198
1102	82-83	0.22135098626901595
1102	84-85	0.16268164963826592
1102	86-87	0.152620041686653
1102	88-89	0.14387731882763433
1102	90-91	0.1453407681834733
1102	92-93	0.1500787099936005
1102	94-95	0.1661777253863903
1102	96-97	0.13402795972276849
1102	98-99	0.2461314165775903
1102	100	0.2811092237764754
1103	1	0.23397035060938975
1103	2	0.1959648243720622
1103	3	0.31563217297448176
1103	4	0.19647235060596557
1103	5	0.22953338320197503
1103	6	0.1982978140662084
1103	7	0.18321114955868012
1103	8	0.21228675654639062
1103	9	0.18959604450598277
1103	10-11	0.24998656912376305
1103	12-13	0.2362455051323309
1103	14-15	0.3641764919807642
1103	16-17	0.3193907135205123
1103	18-19	0.2742276649072224
1103	20-21	0.3092746618892548
1103	22-23	0.3077791952078641
1103	24-25	0.31128299552135985
1103	26-27	0.29385884548629093
1103	28-29	0.31271939387138303
1103	30-31	0.31460101035504096
1103	32-33	0.34151108164198973
1103	34-35	0.3285663337663962
1103	36-37	0.2984922109921513
1103	38-39	0.2737595242938795
1103	40-41	0.24462365708025402
1103	42-43	0.2811231121118354
1103	44-45	0.28486433802172684
1103	46-47	0.22787552474161998
1103	48-49	0.20483308611157724
1103	50-51	0.2068267521552869
1103	52-53	0.20842924101373939
1103	54-55	0.17147760270891865
1103	56-57	0.1861795800807684
1103	58-59	0.21257565748830842
1103	60-61	0.1590430891124015
1103	62-63	0.21525616305125794
1103	64-65	0.1719571122529544
1103	66-67	0.18827980064447303
1103	68-69	0.20383360489037017
1103	70-71	0.15122896526104768
1103	72-73	0.14693123568706312
1103	74-75	0.18418563388696896
1103	76-77	0.16334571901656858
1103	78-79	0.154702432225875
1103	80-81	0.15354232409811885
1103	82-83	0.1412284932222576
1103	84-85	0.1557942300967241
1103	86-87	0.1050616431510889
1103	88-89	0.1382531292244522
1103	90-91	0.16132543371162456
1103	92-93	0.1668488613682868
1103	94-95	0.16836373192625587
1103	96-97	0.2052138687804934
1103	98-99	0.21032626503363971
1103	100	0.15296188624229856
1104	1	0.16076665473949703
1104	2	0.15400508151808623
1104	3	0.2597003866191727
1104	4	0.15771767108812895
1104	5	0.16494996378479954
1104	6	0.12458962138413199
1104	7	0.1414604091856333
1104	8	0.13028028907034184
1104	9	0.18634178668854418
1104	10-11	0.2056875624156902
1104	12-13	0.18989646069810107
1104	14-15	0.31470633447577967
1104	16-17	0.2681450016654594
1104	18-19	0.23049915825676948
1104	20-21	0.23933266148104337
1104	22-23	0.2688434394582302
1104	24-25	0.2690812953413797
1104	26-27	0.2686935761975775
1104	28-29	0.24900908684012535
1104	30-31	0.27860947804284564
1104	32-33	0.2961668598261795
1104	34-35	0.2888386092380344
1104	36-37	0.28585572359349953
1104	38-39	0.24542547552258043
1104	40-41	0.26555078672181764
1104	42-43	0.28451131769205773
1104	44-45	0.2915881243408549
1104	46-47	0.27395810036819057
1104	48-49	0.26001048172298624
1104	50-51	0.29712482081581726
1104	52-53	0.329671379057352
1104	54-55	0.2872393975730745
1104	56-57	0.2539998482382515
1104	58-59	0.2780530287285785
1104	60-61	0.1592341211845536
1104	62-63	0.2080353955269132
1104	64-65	0.18860264582284714
1104	66-67	0.2058545173941866
1104	68-69	0.2153492767240408
1104	70-71	0.2629360044065976
1104	72-73	0.2091394114512326
1104	74-75	0.1955754037113735
1104	76-77	0.18854391196527587
1104	78-79	0.18609240943113292
1104	80-81	0.15547242396369398
1104	82-83	0.1571462369310197
1104	84-85	0.1421476264104271
1104	86-87	0.22026640212074255
1104	88-89	0.1687682476786776
1104	90-91	0.17969051320787344
1104	92-93	0.18854541296767735
1104	94-95	0.12710584073384723
1104	96-97	0.1518591949695285
1104	98-99	0.1953309685250133
1104	100	0.14942869337701126
1105	1	0.10882714175945196
1105	2	0.12758338429120641
1105	3	0.25997880344003477
1105	4	0.129305644244873
1105	5	0.1532615466970455
1105	6	0.12845475452510868
1105	7	0.1406716296879722
1105	8	0.08083294062816293
1105	9	0.17500023566889666
1105	10-11	0.15275283918649052
1105	12-13	0.16410651412486743
1105	14-15	0.1609184767330376
1105	16-17	0.1867224939231491
1105	18-19	0.1214193300151507
1105	20-21	0.146310846392403
1105	22-23	0.12018241367196936
1105	24-25	0.16750584632173826
1105	26-27	0.18164884662147784
1105	28-29	0.22003008544542269
1105	30-31	0.2275006790019276
1105	32-33	0.2038232587826272
1105	34-35	0.16109906784829064
1105	36-37	0.1896605156709228
1105	38-39	0.20825575450075462
1105	40-41	0.24453086961160153
1105	42-43	0.21733156239532292
1105	44-45	0.22031738671062584
1105	46-47	0.21099585778485164
1105	48-49	0.20378266657237276
1105	50-51	0.17494106656227615
1105	52-53	0.14854010703344045
1105	54-55	0.26327392865059096
1105	56-57	0.23543185768730268
1105	58-59	0.2563728240357932
1105	60-61	0.19657735097538875
1105	62-63	0.20072329219438956
1105	64-65	0.23185788208423475
1105	66-67	0.21898698790852933
1105	68-69	0.23800231198845267
1105	70-71	0.2621176579847031
1105	72-73	0.27114244818034905
1105	74-75	0.2554430556755918
1105	76-77	0.24173911040583818
1105	78-79	0.2121021388274329
1105	80-81	0.23367449232151927
1105	82-83	0.19501896762035642
1105	84-85	0.22552884442774968
1105	86-87	0.22388036787563692
1105	88-89	0.13552495966584388
1105	90-91	0.149300948381196
1105	92-93	0.11491484247660111
1105	94-95	0.10838146508595514
1105	96-97	0.16727373369869625
1105	98-99	0.19571954182973172
1105	100	0.1729106931032156
1106	1	0.10415204245726173
1106	2	0.10758818497982503
1106	3	0.1954004142381578
1106	4	0.09140773344243769
1106	5	0.10099821192321201
1106	6	0.09545796256576722
1106	7	0.10523137408111438
1106	8	0.062383044852865055
1106	9	0.07797359317202535
1106	10-11	0.10236907484745217
1106	12-13	0.07403435584434703
1106	14-15	0.15043422033445353
1106	16-17	0.1323766966860731
1106	18-19	0.08784150162968984
1106	20-21	0.0766019917423435
1106	22-23	0.1721181209539111
1106	24-25	0.10262420846987652
1106	26-27	0.11331768360866334
1106	28-29	0.1377282119328953
1106	30-31	0.16252970358841168
1106	32-33	0.15360017986591146
1106	34-35	0.1427395254197208
1106	36-37	0.16111734506267794
1106	38-39	0.15575025803616427
1106	40-41	0.14731071269636686
1106	42-43	0.128640017974341
1106	44-45	0.2012213780172729
1106	46-47	0.1561489767124442
1106	48-49	0.19382674354797302
1106	50-51	0.17236925331745567
1106	52-53	0.21574859198926077
1106	54-55	0.2605080857341022
1106	56-57	0.30112584389367925
1106	58-59	0.2215887582294016
1106	60-61	0.20701099477827967
1106	62-63	0.21731832025030684
1106	64-65	0.24420448473583178
1106	66-67	0.19816156095308202
1106	68-69	0.2417106930471178
1106	70-71	0.30419514898967037
1106	72-73	0.27291759885329725
1106	74-75	0.24710696389691122
1106	76-77	0.2694159929997042
1106	78-79	0.2122722313028902
1106	80-81	0.20910395410779614
1106	82-83	0.26360973732855086
1106	84-85	0.24568068074267302
1106	86-87	0.2394275326538633
1106	88-89	0.21875526810162427
1106	90-91	0.23950748472263683
1106	92-93	0.2579773800210816
1106	94-95	0.22234887060377417
1106	96-97	0.22145050577073633
1106	98-99	0.20265718462390225
1106	100	0.14278824904481624
1107	1	0.07439801053519446
1107	2	0.06558368961533034
1107	3	0.12197252025257654
1107	4	0.07403619059308397
1107	5	0.08435800449146313
1107	6	0.040251337043486046
1107	7	0.011775624851892985
1107	8	0.0574022156236893
1107	9	0.07624254236704786
1107	10-11	0.08428978804558085
1107	12-13	0.10916183091910625
1107	14-15	0.14323557695191624
1107	16-17	0.13798031894992135
1107	18-19	0.1537752096128031
1107	20-21	0.13317443323172284
1107	22-23	0.1050145793122752
1107	24-25	0.08445867213739433
1107	26-27	0.09775380971694148
1107	28-29	0.15594439416289418
1107	30-31	0.15436846349981437
1107	32-33	0.17730916940450925
1107	34-35	0.14802866696093275
1107	36-37	0.19867885331482427
1107	38-39	0.1672312934201159
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2216	60-61	-0.7026234269743981
2216	62-63	-0.6868026319935154
2216	64-65	-0.7371498278320772
2216	66-67	-0.6750050156405081
2216	68-69	-0.6637156330996987
2216	70-71	-0.6734142742122771
2216	72-73	-0.6694855009071574
2216	74-75	-0.6840205764598863
2216	76-77	-0.645326413590773
2216	78-79	-0.6115573329865356
2216	80-81	-0.5588563676120302
2216	82-83	-0.5981963805343966
2216	84-85	-0.6874853473090141
2216	86-87	-0.6397512814110016
2216	88-89	-0.6249913229909296
2216	90-91	-0.5751626452381586
2216	92-93	-0.5929403679899821
2216	94-95	-0.644129110113731
2216	96-97	-0.64472266018252
2216	98-99	-0.701457378353382
2216	100	-0.7469299562507317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	414.0
3	36.0
4	40.0
5	179.0
6	551.0
7	1388.0
8	2902.0
9	4923.0
10	5699.0
11	6660.0
12	9455.0
13	13562.0
14	14854.0
15	18759.0
16	23991.0
17	34410.0
18	34918.0
19	18369.0
20	13388.0
21	12552.0
22	13118.0
23	14290.0
24	16388.0
25	20401.0
26	25910.0
27	33357.0
28	43856.0
29	57417.0
30	74825.0
31	98325.0
32	127938.0
33	170384.0
34	236932.0
35	361044.0
36	583763.0
37	1116495.0
38	1629064.0
39	675495.0
40	2182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.97214622957316	20.61281705989914	14.628527489259726	22.786509221267977
2	15.423611974410653	21.29172122820453	39.651055029561995	23.63361176782282
3	17.840091594521002	26.333606005109605	31.05089418100066	24.77540821936873
4	12.716024728201086	17.944327841117286	38.24305022222689	31.096597208454735
5	12.458743048324846	37.67402409219301	35.55087353003278	14.316359329449366
6	29.374855931699667	38.57730469412578	17.905660090145076	14.142179284029478
7	25.54933335556267	33.29570656119331	23.168336101731096	17.986623981512924
8	24.534606542600404	35.82747668910017	20.895072590252607	18.742844178046816
9	24.654246267918325	16.72991757870362	22.24615701327635	36.36967914010171
10-11	23.35782607261671	27.186043578434692	29.145927845756454	20.310202503192144
12-13	24.288241491752615	24.89362538812236	29.47807215134407	21.340060968780953
14-15	21.561789514543964	26.7033076161685	27.477531761067038	24.257371108220493
16-17	20.353812469714043	29.861356006287522	28.18138012994737	21.603451394051067
18-19	20.22670658765105	27.99181767210307	31.326888276213005	20.454587464032876
20-21	21.2239368609595	27.23757274519348	31.250595752191735	20.28789464165528
22-23	20.983144245061013	27.13326401163851	31.320382571670574	20.56320917162991
24-25	20.832498223163427	27.15631486450194	31.281212431368445	20.72997448096619
26-27	20.59820225093753	27.240916206163057	31.279246222613978	20.881635320285437
28-29	20.68235960997667	27.191742865561704	31.40927876563408	20.71661875882755
30-31	20.914046051689724	27.393410645507238	31.110415034955025	20.58212826784801
32-33	20.608250755585935	27.30622695594279	31.36949248618308	20.716029802288197
34-35	20.862426276232576	27.074359296833006	31.22494442968529	20.83826999724912
36-37	21.147970528252337	27.249478728158323	30.82544161773495	20.777109125854395
38-39	20.95623346164733	27.400804315293627	30.866306140696466	20.77665608236258
40-41	20.887543007418678	27.206466779045613	30.930393673048297	20.97559654048741
42-43	20.84526498876271	27.354621061738232	30.730882380123788	21.069231569375273
44-45	21.09639605714437	27.462934699760826	30.448754076032298	20.991915167062505
46-47	21.197633155824853	27.5798380423882	30.061546864449753	21.160981937337198
48-49	21.254807244491623	27.59251419928912	29.807208248146054	21.3454703080732
50-51	21.298670915368938	27.68843256737572	29.62019189472574	21.3927046225296
52-53	21.296813437052506	27.81399810156655	29.47224601203936	21.416942449341583
54-55	21.363818569491617	28.058750317583485	29.36840844371587	21.209022669209027
56-57	21.259600444635005	27.869477807573944	29.38006978319513	21.49085196459592
58-59	21.216697225960335	27.520643379747938	29.684261305337905	21.578398088953822
60-61	21.380608361298197	27.83781912836607	29.415443419035874	21.36612909129986
62-63	21.19166204260276	27.877016451277708	29.50156698682948	21.429754519290046
64-65	21.226292687116928	27.656782949037684	29.609889685721917	21.50703467812347
66-67	21.184132459768833	27.694838602349954	29.541579788026382	21.579449149854828
68-69	21.416906205862237	27.854337094077565	29.36842656545554	21.36033013460466
70-71	21.452760067804302	27.615537869543044	29.402096757767065	21.529605304885585
72-73	21.278519540853104	27.802228031649257	29.489479786467914	21.42977264102972
74-75	21.36772380439104	27.864639303081383	29.2791951193081	21.488441773219474
76-77	21.483050555666907	27.84523091989212	29.13268085405584	21.539037670385127
78-79	21.496768712598996	27.876037877335392	29.266084040655038	21.361109369410574
80-81	21.381208881698168	27.805449724404085	29.294008362110706	21.51933303178704
82-83	21.26875631885502	27.87109109897472	29.374215211571443	21.48593737059882
84-85	21.33664732648245	27.810839986901563	29.214454596475637	21.638058090140348
86-87	21.40007163554199	27.929597828861368	29.117709445842994	21.552621089753643
88-89	21.27017367600992	27.852747795207357	29.20852356726081	21.668554961521917
90-91	21.230551406155737	27.78731691723444	29.169733034371166	21.812398642238655
92-93	21.265502717721237	27.92707293974549	29.119376408287735	21.688047934245535
94-95	21.159292097942433	27.71601572318325	29.276011236229937	21.84868094264438
96-97	21.23549699146018	27.84055731130855	29.168515470192908	21.755430227038357
98-99	21.107950241493015	27.940375907093873	29.10702131068042	21.844652540732692
100	21.25391656382572	27.86085393323584	28.9964716947282	21.88875780821024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3538.0
1	2548.5
2	5360.0
3	7431.0
4	3523.0
5	1547.0
6	3227.0
7	7983.0
8	12790.5
9	14094.5
10	10240.0
11	5358.5
12	4178.0
13	4474.5
14	4954.0
15	5474.0
16	6015.0
17	6618.0
18	7613.0
19	8633.5
20	9574.0
21	11466.5
22	14421.0
23	19094.5
24	25523.5
25	33202.0
26	43613.0
27	56760.0
28	71539.0
29	87700.5
30	102855.5
31	117114.0
32	130566.0
33	141827.0
34	151939.0
35	161070.0
36	168619.5
37	177404.5
38	186704.5
39	191606.5
40	195067.5
41	196473.0
42	194655.5
43	193421.0
44	190760.5
45	186555.0
46	182616.5
47	176488.0
48	170674.0
49	167462.5
50	163333.5
51	155703.0
52	144517.0
53	135804.0
54	130128.0
55	122852.5
56	112457.5
57	101437.5
58	92297.5
59	83977.0
60	74381.0
61	63249.0
62	53235.0
63	44066.0
64	35987.5
65	29468.5
66	23759.5
67	18739.5
68	14415.0
69	10863.0
70	7986.0
71	5798.5
72	4219.5
73	2963.0
74	2159.5
75	1518.5
76	920.5
77	590.5
78	384.0
79	202.5
80	98.5
81	48.5
82	18.5
83	13.0
84	8.5
85	5.0
86	3.5
87	3.0
88	2.5
89	1.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.5
95	1.0
96	0.5
97	0.0
98	0.5
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12206803843403523
2	0.0
3	0.0
4	0.0
5	0.012304661237635083
6	3.2619131410520103E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.812173967251117E-4
82-83	4.892869711578015E-4
84-85	3.0806957443268984E-4
86-87	4.258608823040124E-4
88-89	5.074087108303128E-4
90-91	5.980174091928687E-4
92-93	1.4497391738008936E-4
94-95	2.355826157426452E-4
96-97	4.6210436164903485E-4
98-99	0.0011235478596956924
100	7.248695869004468E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5518234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.593124328125555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28848661784268	41.47624434983737
2	15.788212477814891	17.87008543351683
3	4.899042814272467	8.317564172307955
4	2.0866459637837167	4.723592578287731
5	1.0532143378358334	2.9802344982653874
6	0.6701169118880601	2.2754405825316324
7	0.4378888720667181	1.7347049565142114
8	0.3039084546682169	1.375930316752553
9	0.22533568960648645	1.147720562771742
>10	1.1663861851397	11.54908255606891
>50	0.05664321514950283	2.1747266779459498
>100	0.022742506243545662	2.220344833940233
>500	8.319761397953114E-4	0.30566586990374983
>1k	3.5198547262226194E-4	0.3696238680038904
>5k	6.399735864038615E-5	0.20756595827680396
>10k+	1.279947172807723E-4	1.2714727850751144
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23820	0.43165983899921606	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22039	0.39938502064247366	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14180	0.2569662685562084	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10173	0.18435245768845612	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5941	0.10766125539438887	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5521	0.10005012473193417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.812173967251117E-5	0.0
4	0.0	0.0	0.0	1.812173967251117E-5	0.0
5	0.0	1.812173967251117E-5	0.0	3.624347934502234E-5	0.0
6	0.0	3.624347934502234E-5	0.0	3.624347934502234E-5	0.0
7	0.0	3.624347934502234E-5	0.0	3.624347934502234E-5	0.0
8	0.0	3.624347934502234E-5	0.0	5.4365219017533505E-5	0.0
9	0.0	3.624347934502234E-5	0.0	7.248695869004468E-5	0.0
10-11	0.0	4.5304349181277926E-5	0.0	9.966956819881143E-5	0.0
12-13	0.0	5.4365219017533505E-5	0.0	1.0873043803506701E-4	0.0
14-15	0.0	7.248695869004468E-5	0.0	1.268521777075782E-4	0.0
16-17	0.0	1.268521777075782E-4	0.0	1.268521777075782E-4	0.0
18-19	0.0	1.3591304754383376E-4	0.0	1.268521777075782E-4	0.0
20-21	0.0	1.4497391738008936E-4	0.0	1.6309565705260052E-4	0.0
22-23	0.0	1.4497391738008936E-4	0.0	1.9933913639762286E-4	0.0
24-25	0.0	1.4497391738008936E-4	0.0	3.7149566328647897E-4	0.0
26-27	0.0	1.4497391738008936E-4	0.0	7.792348059179803E-4	0.0
28-29	0.0	1.4497391738008936E-4	0.0	0.0015856522213447274	0.0
30-31	0.0	1.5403478721634495E-4	0.0	0.0035881044551572113	0.0
32-33	0.0	1.6309565705260052E-4	0.0	0.007647374141799714	0.0
34-35	0.0	1.812173967251117E-4	0.0	0.013690974322582188	0.0
36-37	0.0	2.355826157426452E-4	0.0	0.023558261574264522	0.0
38-39	1.812173967251117E-5	3.080695744326899E-4	0.0	0.035708888024683264	0.0
40-41	1.812173967251117E-5	3.3525218394145665E-4	0.0	0.048412227535113594	0.0
42-43	1.812173967251117E-5	3.7149566328647897E-4	0.0	0.0611789931343977	0.0
44-45	1.812173967251117E-5	3.986782727952457E-4	0.0	0.07694490664948242	0.0
46-47	1.812173967251117E-5	4.5304349181277923E-4	0.0	0.09663417680366582	0.0
48-49	1.812173967251117E-5	4.5304349181277923E-4	0.0	0.11633250782768545	0.0
50-51	1.812173967251117E-5	4.711652314852904E-4	0.0	0.13649294321335412	0.0
52-53	1.812173967251117E-5	4.711652314852904E-4	0.0	0.1571426655701806	0.0
54-55	1.812173967251117E-5	4.892869711578015E-4	0.0	0.17982202277032833	0.0
56-57	1.812173967251117E-5	5.074087108303128E-4	0.0	0.20814630187846328	0.0
58-59	1.812173967251117E-5	5.074087108303128E-4	0.0	0.23747633753842262	0.0
60-61	2.7182609508766753E-5	5.074087108303128E-4	0.0	0.26946120806040486	0.0
62-63	3.624347934502234E-5	5.074087108303128E-4	0.0	0.30146420032205956	0.0
64-65	3.624347934502234E-5	5.074087108303128E-4	0.0	0.3376261318385556	0.0
66-67	3.624347934502234E-5	5.074087108303128E-4	0.0	0.3803028287673194	0.0
68-69	3.624347934502234E-5	5.255304505028239E-4	0.0	0.42857914325488916	0.0
70-71	3.624347934502234E-5	5.43652190175335E-4	0.0	0.481431197009768	0.0
72-73	3.624347934502234E-5	5.980174091928686E-4	0.0	0.5373730073788099	0.0
74-75	3.624347934502234E-5	5.980174091928686E-4	0.0	0.5954260004196994	0.0
76-77	3.624347934502234E-5	5.980174091928686E-4	0.0	0.661914663278143	0.0
78-79	3.624347934502234E-5	5.980174091928686E-4	0.0	0.7396297438637072	0.0
80-81	3.624347934502234E-5	6.252000187016353E-4	0.0	0.8240226855185917	0.0
82-83	3.624347934502234E-5	6.433217583741466E-4	0.0	0.9125745664283175	0.0
84-85	3.624347934502234E-5	6.523826282104021E-4	0.0	1.0070250736014457	0.0
86-87	3.624347934502234E-5	6.523826282104021E-4	0.0	1.1066312157114033	0.0
88	3.624347934502234E-5	6.523826282104021E-4	0.0	1.1928453922033753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10845	0.0	57.37026	1
GTATCAA	19985	0.0	40.81119	1
ATCAACG	24515	0.0	33.09408	3
TCAACGC	24865	0.0	32.76058	4
TATCAAC	25655	0.0	31.696808	2
CAACGCA	25785	0.0	31.573462	5
GTGGTAT	4530	0.0	30.54457	1
AACGCAG	27145	0.0	30.126568	6
ACGCAGA	30595	0.0	26.590897	7
TGGTATC	4980	0.0	26.33397	2
CGCAGAG	30975	0.0	26.219158	8
ATAACGC	525	0.0	25.069183	3
GCAGAGT	33505	0.0	23.958775	9
GTACCTG	4080	0.0	23.070377	1
GTACATG	15780	0.0	22.905464	1
TATAACG	595	0.0	22.119867	2
TACCTGG	4145	0.0	21.886395	2
GAGTACT	25075	0.0	21.620363	12-13
CAGAGTA	32445	0.0	21.61262	10-11
TACATGG	16020	0.0	21.536491	2
>>END_MODULE