FastQCFastQC Report
Wed 25 May 2016
SRR2049467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911121
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63700.044.9640961
GTATCAA110750.033.6757621
ATCAACG113800.031.1837273
TCAACGC114200.030.906994
CAACGCA123400.028.6791575
AACGCAG127950.027.8064676
TATCAAC130050.027.7207282
GTACATG180700.024.1013181
ACGCAGA146900.023.6119677
CGCAGAG149750.022.9097588
TACATGG182600.022.8834022
ACATGGG185450.021.7208753
GTGGTAT35550.020.7705521
GCAGAGT174450.018.9391259
TGGTATC36650.018.7239952
GTACCGT3559.094947E-1118.5348856
CATGGGG119000.018.4044084
CAGAGTA168650.017.04106110-11
AGAGTAC157850.016.37588110-11
GTATAGG20250.016.0255171
Produced by FastQC (version 0.11.5)