##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049467_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5911121
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.69090093063566	31.0	31.0	34.0	28.0	34.0
2	30.924951460137596	31.0	31.0	34.0	28.0	34.0
3	31.051448109419518	31.0	31.0	34.0	28.0	34.0
4	34.67043966787349	37.0	35.0	37.0	32.0	37.0
5	34.699215258831615	37.0	35.0	37.0	32.0	37.0
6	34.70238403172596	37.0	35.0	37.0	32.0	37.0
7	34.58087848311683	37.0	35.0	37.0	32.0	37.0
8	34.55188973462056	37.0	35.0	37.0	32.0	37.0
9	36.13279765377836	39.0	37.0	39.0	32.0	39.0
10-11	36.072123206410424	39.0	37.0	39.0	31.5	39.0
12-13	35.99638757183282	39.0	37.0	39.0	31.0	39.0
14-15	37.20376684896148	40.0	38.0	41.0	31.0	41.0
16-17	37.19213301504063	40.0	38.0	41.0	31.0	41.0
18-19	37.21636124518514	40.0	38.0	41.0	31.0	41.0
20-21	37.263479888163346	40.0	38.0	41.0	31.0	41.0
22-23	37.227270343476306	40.0	38.0	41.0	31.0	41.0
24-25	37.22676815446681	40.0	37.0	41.0	31.0	41.0
26-27	37.225525327598604	40.0	37.0	41.0	31.0	41.0
28-29	37.20487196929314	40.0	37.0	41.0	31.0	41.0
30-31	37.14201037332852	40.0	37.0	41.0	31.0	41.0
32-33	37.09315102837516	40.0	37.0	41.0	31.0	41.0
34-35	37.0242405797479	40.0	37.0	41.0	30.5	41.0
36-37	36.91267603894422	40.0	37.0	41.0	30.0	41.0
38-39	36.804270628193876	40.0	37.0	41.0	30.0	41.0
40-41	36.680879565821776	40.0	37.0	41.0	30.0	41.0
42-43	36.5857460877556	40.0	36.5	41.0	30.0	41.0
44-45	36.4320423317337	40.0	36.0	41.0	30.0	41.0
46-47	36.312104083134145	40.0	36.0	41.0	30.0	41.0
48-49	36.211983818297746	40.0	36.0	41.0	29.0	41.0
50-51	36.09998983272378	40.0	36.0	41.0	29.0	41.0
52-53	35.98843975618161	39.0	35.0	41.0	29.0	41.0
54-55	35.84009293668663	39.0	35.0	41.0	28.0	41.0
56-57	35.678914371740994	39.0	35.0	41.0	28.0	41.0
58-59	35.49484784696507	39.0	35.0	41.0	28.0	41.0
60-61	35.29770782902261	39.0	35.0	40.0	28.0	41.0
62-63	35.043515688479395	38.0	34.0	40.0	27.5	41.0
64-65	34.80203441952889	38.0	34.0	40.0	27.0	41.0
66-67	34.574105909860414	37.5	34.0	40.0	26.5	41.0
68-69	34.287828569234165	37.0	34.0	40.0	26.0	41.0
70-71	33.97019803857847	36.5	34.0	39.0	26.0	41.0
72-73	33.630329509411155	36.0	33.5	39.0	26.0	41.0
74-75	33.32369325547557	35.5	33.0	39.0	26.0	40.5
76-77	32.988629144962516	35.0	33.0	38.0	25.0	40.0
78-79	32.63743239226536	35.0	33.0	37.0	25.0	39.0
80-81	32.29557193973867	35.0	33.0	37.0	24.5	39.0
82-83	31.984485853021788	35.0	33.0	36.0	24.0	39.0
84-85	31.683165257486692	35.0	32.0	36.0	23.0	37.0
86-87	31.397014035070505	35.0	32.0	36.0	22.0	37.0
88-89	31.150279109495475	35.0	32.0	35.0	20.5	37.0
90-91	30.92213913401536	35.0	32.0	35.0	19.5	36.0
92-93	30.695502426697068	35.0	32.0	35.0	18.0	36.0
94-95	30.468998266149516	35.0	32.0	35.0	12.5	36.0
96-97	30.257418601310988	35.0	32.0	35.0	2.0	35.5
98-99	30.05427566108019	35.0	32.0	35.0	2.0	35.0
100	29.899717498592906	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20783464474166635
1101	2	0.24063978699279076
1101	3	0.3499047068845762
1101	4	0.3579232266578174
1101	5	0.32660145715280464
1101	6	0.3475527972180501
1101	7	0.3983633780692557
1101	8	0.40106082669095855
1101	9	0.5159159250697698
1101	10-11	0.5421696793967428
1101	12-13	0.5222388301952279
1101	14-15	0.6070744857206734
1101	16-17	0.5453072761067332
1101	18-19	0.5213847128552587
1101	20-21	0.5417457987137411
1101	22-23	0.5043066702984831
1101	24-25	0.5047709955937094
1101	26-27	0.5013830786477769
1101	28-29	0.476690326745306
1101	30-31	0.4925980743554348
1101	32-33	0.49642329488382586
1101	34-35	0.5021340974368016
1101	36-37	0.5143578287867285
1101	38-39	0.5895826343200099
1101	40-41	0.5830953359429003
1101	42-43	0.4948793486721854
1101	44-45	0.5428878953626182
1101	46-47	0.4505949039994377
1101	48-49	0.4077535974511619
1101	50-51	0.41891245442623415
1101	52-53	0.39421414603823024
1101	54-55	0.3646032078391457
1101	56-57	0.3635318581986908
1101	58-59	0.42106770802877236
1101	60-61	0.4174124937980608
1101	62-63	0.3932069615997662
1101	64-65	0.4304525912503294
1101	66-67	0.43533108082231564
1101	68-69	0.3717596137937207
1101	70-71	0.3635174950708233
1101	72-73	0.4032374882611265
1101	74-75	0.37713685444929723
1101	76-77	0.36804775276905843
1101	78-79	0.34340727649711056
1101	80-81	0.404481600063761
1101	82-83	0.4047256442936593
1101	84-85	0.35553575778051894
1101	86-87	0.29606556978063026
1101	88-89	0.25553752232464433
1101	90-91	0.30965358215296845
1101	92-93	0.2719599712464351
1101	94-95	0.2618207809245483
1101	96-97	0.28505935564125906
1101	98-99	0.33610158773397814
1101	100	0.28753276218795065
1102	1	0.20497269346531155
1102	2	0.2737091495545627
1102	3	0.31171135169860875
1102	4	0.3123648757271411
1102	5	0.3033118887195769
1102	6	0.2941535964923503
1102	7	0.32969421397459087
1102	8	0.3806220634533872
1102	9	0.46944585260296634
1102	10-11	0.4446216332405939
1102	12-13	0.4583784336299388
1102	14-15	0.5261598333226232
1102	16-17	0.4537887162383143
1102	18-19	0.5324615067319627
1102	20-21	0.5119320530660687
1102	22-23	0.48816494409270206
1102	24-25	0.4521544447896204
1102	26-27	0.39233227720835373
1102	28-29	0.46888157766269245
1102	30-31	0.39368729444019834
1102	32-33	0.42379611573448983
1102	34-35	0.38634907089213755
1102	36-37	0.4699515088920947
1102	38-39	0.49278471939503277
1102	40-41	0.5088068055998889
1102	42-43	0.457612553242285
1102	44-45	0.4342533025091697
1102	46-47	0.4270745420326918
1102	48-49	0.34010000849955446
1102	50-51	0.38229505752316584
1102	52-53	0.33083519049621657
1102	54-55	0.3730764165901306
1102	56-57	0.2976475980975408
1102	58-59	0.3470865308610911
1102	60-61	0.35405121872655343
1102	62-63	0.3100123226639582
1102	64-65	0.3221129097596105
1102	66-67	0.3217647513041513
1102	68-69	0.31117074517273835
1102	70-71	0.26660973552054656
1102	72-73	0.28091592628504003
1102	74-75	0.29588167535323606
1102	76-77	0.2747199610812743
1102	78-79	0.3073092312242949
1102	80-81	0.3265419619981813
1102	82-83	0.34381661843714895
1102	84-85	0.26346602422144727
1102	86-87	0.23334980604031585
1102	88-89	0.22624056470867515
1102	90-91	0.23530834612440188
1102	92-93	0.24820334023403845
1102	94-95	0.21379360098384126
1102	96-97	0.21714823649505988
1102	98-99	0.24667977283660747
1102	100	0.2673204037146135
1103	1	0.14389148892210457
1103	2	0.2406438033239624
1103	3	0.25148580442354174
1103	4	0.2639741981918249
1103	5	0.23575977608603438
1103	6	0.23907276509620345
1103	7	0.2439024335337976
1103	8	0.22274826677610093
1103	9	0.3278658252446789
1103	10-11	0.3468637609531342
1103	12-13	0.3325537534733982
1103	14-15	0.42191966550705473
1103	16-17	0.3657998301588492
1103	18-19	0.3388625427071901
1103	20-21	0.36284051359604064
1103	22-23	0.3887703019245521
1103	24-25	0.34208256914164537
1103	26-27	0.3886846471296366
1103	28-29	0.31756259795087516
1103	30-31	0.3509206167788932
1103	32-33	0.41868010768185826
1103	34-35	0.3761925153588379
1103	36-37	0.35360147770173
1103	38-39	0.41692266164866965
1103	40-41	0.4283416256231476
1103	42-43	0.37508626070111006
1103	44-45	0.3447590600414472
1103	46-47	0.33015033135027494
1103	48-49	0.2929697822534507
1103	50-51	0.3030066523571975
1103	52-53	0.3081710041492869
1103	54-55	0.2604779378096467
1103	56-57	0.22203718495418912
1103	58-59	0.2924505203981056
1103	60-61	0.2527156404229771
1103	62-63	0.2619715661903186
1103	64-65	0.33031622580336517
1103	66-67	0.271310937986172
1103	68-69	0.272877444037384
1103	70-71	0.22822611409551996
1103	72-73	0.20357492335060812
1103	74-75	0.26064849179637406
1103	76-77	0.17178325161279417
1103	78-79	0.1948596093583106
1103	80-81	0.25657440020144406
1103	82-83	0.1979256324977534
1103	84-85	0.1881396135024609
1103	86-87	0.24715470251278404
1103	88-89	0.2038561292937402
1103	90-91	0.1819496972273491
1103	92-93	0.09846263478923944
1103	94-95	0.15415290048225216
1103	96-97	0.21389655569284116
1103	98-99	0.17717101678554315
1103	100	0.14773018852293518
1104	1	0.13577840879109715
1104	2	0.08149811106325444
1104	3	0.19373573070596706
1104	4	0.24067612643302283
1104	5	0.2266631455058743
1104	6	0.28765511026296764
1104	7	0.24428697601091898
1104	8	0.2455433213797633
1104	9	0.3227842751746479
1104	10-11	0.3973819494489774
1104	12-13	0.38088698588385483
1104	14-15	0.42030673807674646
1104	16-17	0.35534830999709044
1104	18-19	0.35113628239989936
1104	20-21	0.3547358122575375
1104	22-23	0.36390933854112717
1104	24-25	0.3847441159470435
1104	26-27	0.3740557822223636
1104	28-29	0.29402751101678604
1104	30-31	0.28799445019823366
1104	32-33	0.27031105642707587
1104	34-35	0.35626528513884637
1104	36-37	0.38276641551568247
1104	38-39	0.38569047892386266
1104	40-41	0.3627103069736535
1104	42-43	0.3478141204165581
1104	44-45	0.3328432323510171
1104	46-47	0.31481256004325786
1104	48-49	0.3350694497345259
1104	50-51	0.330107293963394
1104	52-53	0.31397604616829966
1104	54-55	0.28485493871531276
1104	56-57	0.2539541644817618
1104	58-59	0.2600766761844113
1104	60-61	0.3035819865059395
1104	62-63	0.3398141357217881
1104	64-65	0.3097897835716168
1104	66-67	0.3307508950193565
1104	68-69	0.35881318995395617
1104	70-71	0.2908025434266861
1104	72-73	0.30318150185456716
1104	74-75	0.30874293849331025
1104	76-77	0.2992021688595514
1104	78-79	0.32968317423505056
1104	80-81	0.2769691446647258
1104	82-83	0.3042685561368721
1104	84-85	0.26438440061663115
1104	86-87	0.263364993749871
1104	88-89	0.26823431494619143
1104	90-91	0.26891197357348773
1104	92-93	0.2882522369249436
1104	94-95	0.2701555911738325
1104	96-97	0.2861506746624798
1104	98-99	0.3128457695683835
1104	100	0.25023315038988514
1105	1	0.18796618374901186
1105	2	0.25843110652152035
1105	3	0.2648200248556236
1105	4	0.27878151700677023
1105	5	0.33713117791339897
1105	6	0.36214749663064794
1105	7	0.32778749223248127
1105	8	0.2960060771383013
1105	9	0.3820759708734798
1105	10-11	0.39490735365300367
1105	12-13	0.4202317850151829
1105	14-15	0.5082912425294737
1105	16-17	0.468667481143612
1105	18-19	0.465470165860971
1105	20-21	0.4783406662660141
1105	22-23	0.46222353819425877
1105	24-25	0.4426958352445709
1105	26-27	0.45297257518954837
1105	28-29	0.5124476950256138
1105	30-31	0.4937106313284261
1105	32-33	0.49076202555041704
1105	34-35	0.4496344890895898
1105	36-37	0.40132085527542927
1105	38-39	0.502793159970139
1105	40-41	0.4746274279831084
1105	42-43	0.41380447114207186
1105	44-45	0.39644451985336815
1105	46-47	0.37279739508122844
1105	48-49	0.40191701433850113
1105	50-51	0.3689789556882417
1105	52-53	0.33333474895182746
1105	54-55	0.2835316250814728
1105	56-57	0.28029026922133937
1105	58-59	0.2980453951920481
1105	60-61	0.29369866402257827
1105	62-63	0.32829571958109227
1105	64-65	0.3343408106745187
1105	66-67	0.2859551585517366
1105	68-69	0.24023850184840967
1105	70-71	0.28412730909261086
1105	72-73	0.2986233480968252
1105	74-75	0.3156293913645669
1105	76-77	0.26743276223461976
1105	78-79	0.25225163080886404
1105	80-81	0.27672329096649406
1105	82-83	0.2121571018669819
1105	84-85	0.20179877904009302
1105	86-87	0.23224779365425263
1105	88-89	0.238665404453414
1105	90-91	0.28888385475606526
1105	92-93	0.2688723576139864
1105	94-95	0.2698719426825704
1105	96-97	0.2923083333052503
1105	98-99	0.3064927032631566
1105	100	0.22890326955315032
1106	1	0.16812109915078466
1106	2	0.17501093697886105
1106	3	0.18859568747166477
1106	4	0.20937605385559266
1106	5	0.18145973358600997
1106	6	0.2242689436782399
1106	7	0.2791828312930704
1106	8	0.1899162289254761
1106	9	0.30553706706470507
1106	10-11	0.2888661970238786
1106	12-13	0.31877459231125016
1106	14-15	0.37423723542728027
1106	16-17	0.31192234288325693
1106	18-19	0.2750796563922009
1106	20-21	0.28236206187708746
1106	22-23	0.29870499013821217
1106	24-25	0.34632599187865054
1106	26-27	0.38091068197965683
1106	28-29	0.3308720314204052
1106	30-31	0.27008577290248326
1106	32-33	0.24427167731533217
1106	34-35	0.3145276408619253
1106	36-37	0.3627186548807515
1106	38-39	0.33595839111296755
1106	40-41	0.4175779116841625
1106	42-43	0.4234944363235087
1106	44-45	0.2936768583352958
1106	46-47	0.3239398358921477
1106	48-49	0.2967114166610614
1106	50-51	0.30428361811139837
1106	52-53	0.3033477440900896
1106	54-55	0.3108796462594654
1106	56-57	0.2470700776697896
1106	58-59	0.3503306972084559
1106	60-61	0.2692055671488376
1106	62-63	0.27594911414365697
1106	64-65	0.25907345208783283
1106	66-67	0.3214859561638974
1106	68-69	0.318870939741295
1106	70-71	0.3402171634877149
1106	72-73	0.28587607571576257
1106	74-75	0.27894529686975034
1106	76-77	0.2515206667407526
1106	78-79	0.25629287151691216
1106	80-81	0.25660656964852535
1106	82-83	0.26459429615357166
1106	84-85	0.25139397615569337
1106	86-87	0.2535906081217618
1106	88-89	0.2194878755327423
1106	90-91	0.269310759267146
1106	92-93	0.21923811944314764
1106	94-95	0.24680298189796446
1106	96-97	0.34114673362969583
1106	98-99	0.3167802469718275
1106	100	0.3099994978719174
1107	1	0.08598517295973807
1107	2	0.09436977228973831
1107	3	0.10094393732886431
1107	4	0.07020177708646713
1107	5	0.1016778055005716
1107	6	0.14251941546844193
1107	7	0.11763644401297313
1107	8	0.07019937535035581
1107	9	0.0966791247207226
1107	10-11	0.10035331227083333
1107	12-13	0.13539082623242393
1107	14-15	0.16916926871650162
1107	16-17	0.1874752050771349
1107	18-19	0.1462252027650166
1107	20-21	0.16797331036772079
1107	22-23	0.1775006778673358
1107	24-25	0.16620820701497507
1107	26-27	0.21123028820656486
1107	28-29	0.15040573640150257
1107	30-31	0.1291933663747855
1107	32-33	0.07961221584305633
1107	34-35	0.08718792299607969
1107	36-37	0.1463428531989308
1107	38-39	0.17452505271828045
1107	40-41	0.26242448044983746
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2216	70-71	-0.648281663752158
2216	72-73	-0.6529584178712966
2216	74-75	-0.6989828515335716
2216	76-77	-0.6931547237150397
2216	78-79	-0.7331813476567817
2216	80-81	-0.7565324027111657
2216	82-83	-0.7023982079593303
2216	84-85	-0.6561473717396495
2216	86-87	-0.7578059144142202
2216	88-89	-0.7061677502481984
2216	90-91	-0.7305741362872809
2216	92-93	-0.7148100006729585
2216	94-95	-0.7116390491884204
2216	96-97	-0.7380553233335903
2216	98-99	-0.7482435549994122
2216	100	-0.8277143873124224
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97746.0
3	18181.0
4	11427.0
5	9024.0
6	8020.0
7	8369.0
8	9363.0
9	10785.0
10	12067.0
11	13395.0
12	14971.0
13	16519.0
14	18511.0
15	19111.0
16	19430.0
17	20077.0
18	19189.0
19	17933.0
20	17799.0
21	19140.0
22	21844.0
23	25853.0
24	31277.0
25	39048.0
26	47531.0
27	54495.0
28	66039.0
29	83008.0
30	99927.0
31	117610.0
32	149899.0
33	197493.0
34	273433.0
35	398453.0
36	659324.0
37	1171632.0
38	1541379.0
39	550670.0
40	1149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64477712966501	20.850933141862317	14.816870285213824	22.68741944325885
2	15.218686752642748	20.661144579842126	40.65601457516895	23.464154092346178
3	17.984572435762615	25.938977721249035	30.55416762336696	25.52228221962139
4	12.600478268946594	17.65356869372075	39.35357556287288	30.39237747445978
5	12.97278650071837	38.10069238364456	35.6403990392847	13.286122076352369
6	29.859512314624197	38.44332376424028	18.244957954190166	13.45220596694535
7	26.250641285087937	32.856740633853626	23.140137881370045	17.752480199688392
8	24.291998416399068	36.42189091425884	21.084140642711883	18.201970026630214
9	24.481772604080714	17.547090548289585	22.21177579666026	35.75936105096944
10-11	23.239279340494086	27.59919693609395	29.384087003778447	19.777436719633513
12-13	23.78444264925553	25.24042376271765	29.23389001955335	21.74124356847347
14-15	21.408834263988417	27.522420993575537	26.400330799325676	24.668413943110366
16-17	20.819858785526428	30.662133505482693	27.14441892527399	21.373588783716897
18-19	20.750993759553754	29.696572364898188	29.0486047607406	20.503829114807463
20-21	21.918421488973635	28.878481586632333	28.550820759409717	20.652276164984315
22-23	21.62558810058506	28.753552460001885	28.556601048907332	21.064258390505724
24-25	21.365785715264646	28.918534428133956	28.513939960803615	21.201739895797783
26-27	21.138509856679637	28.881132108031142	28.61863865112318	21.36171938416604
28-29	21.2033240337337	28.853105493565273	28.77789996209269	21.165670510608336
30-31	21.504757708848622	29.195105399480553	28.31439504489712	20.985741846773706
32-33	21.133922896674253	28.99572526112571	28.660055348017178	21.21029649418286
34-35	21.358719434941523	28.711827435055554	28.67521530562965	21.254237824373273
36-37	21.571295492336084	29.00584987266849	28.208598311224865	21.214256323770556
38-39	21.401561025677644	29.102121319463226	28.385846793020907	21.11047086183822
40-41	21.36975950389536	28.781737036251226	28.50434304745256	21.344160412400857
42-43	21.230929332959768	28.9051978733973	28.45860555390881	21.405267239734123
44-45	21.37339209749793	28.981509322158512	28.318805012088934	21.326293568254627
46-47	21.374835502730306	28.959378956447512	28.28019717252935	21.385588368292836
48-49	21.25891564263184	28.84200450820102	28.337522194988324	21.561557654178817
50-51	21.31506487294803	28.816002397084045	28.255145977562528	21.613786752405396
52-53	21.310197194918047	28.83731514430408	28.267720831787713	21.584766828990162
54-55	21.386971356563333	29.084663908217422	28.15830411458762	21.370060620631623
56-57	21.28045865316909	28.84394491065524	28.17492624153352	21.70067019464215
58-59	21.260494648188317	28.536865827359854	28.42283245663289	21.779807067818936
60-61	21.38130321631577	28.855753251823273	28.1637650896316	21.599178442229356
62-63	21.25742591177278	28.890732776816826	28.201536424127916	21.65030488728248
64-65	21.35487035240095	28.676531243424513	28.210579448491018	21.758018955683518
66-67	21.181765470142388	28.71697181681888	28.180093324122403	21.921169388916326
68-69	21.462036594229044	28.80352083442077	28.144688339608955	21.589754231741228
70-71	21.476225922647547	28.593152116766284	28.19344154164095	21.73718041894521
72-73	21.263243815477743	28.662166196797262	28.335658921090285	21.73893106663471
74-75	21.326425445591077	28.68805012014466	28.18199754008446	21.803526894179807
76-77	21.38272508527925	28.712341694946453	28.004721024088656	21.900212195685636
78-79	21.47206318268567	28.677950014212044	28.14731629241618	21.702670510686104
80-81	21.323225713989363	28.609464607173233	28.194362873619056	21.872946805218344
82-83	21.245101544994473	28.677496880715285	28.300517925196218	21.776883649094025
84-85	21.280457262779834	28.47073445626066	28.2148497552946	22.0339585256649
86-87	21.41764713944244	28.603587483856185	28.127568141567767	21.851197235133608
88-89	21.318277438000436	28.519766446151202	28.1112214844635	22.05073463138487
90-91	21.238655806978993	28.432876005405873	28.19502600360815	22.133442184006988
92-93	21.337482120178624	28.55247487012201	28.057190388829913	22.052852620869455
94-95	21.21435719456538	28.302814031589175	28.241403473626157	22.24142530021929
96-97	21.338729417306343	28.321700180979576	28.16735192105423	22.172218480659854
98-99	21.307133897957957	28.33324126608177	28.070151692710706	22.289473143249566
100	21.38208321666668	28.225299308120928	28.087177270431752	22.30544020478064
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3820.0
1	2793.5
2	1989.0
3	2183.5
4	2030.5
5	1745.0
6	1517.0
7	1457.0
8	1516.0
9	1561.5
10	1401.0
11	1189.5
12	1177.5
13	1279.5
14	1414.0
15	1608.5
16	1827.0
17	2205.0
18	2861.5
19	3810.5
20	5347.0
21	7616.5
22	10960.0
23	16037.5
24	23015.5
25	32179.5
26	44209.0
27	58053.5
28	74607.0
29	93805.5
30	112011.0
31	129240.0
32	145059.0
33	158825.5
34	171849.0
35	183353.0
36	193365.0
37	202408.0
38	210735.5
39	215614.5
40	218706.0
41	220433.5
42	218437.0
43	216674.5
44	212913.5
45	207821.0
46	203689.5
47	197178.0
48	190895.5
49	185893.0
50	179759.0
51	169876.0
52	155980.5
53	144638.0
54	137646.0
55	130649.0
56	119970.5
57	107189.5
58	96062.5
59	86326.0
60	75008.0
61	62785.0
62	52162.5
63	42672.5
64	34479.5
65	27711.5
66	21977.5
67	17092.5
68	13200.0
69	10168.5
70	7543.5
71	5587.0
72	4146.5
73	2943.5
74	2167.5
75	1550.0
76	997.0
77	662.0
78	463.5
79	306.0
80	202.5
81	158.5
82	119.5
83	88.0
84	72.5
85	54.5
86	44.0
87	37.5
88	30.0
89	24.0
90	21.5
91	15.5
92	10.0
93	13.0
94	9.0
95	6.5
96	9.5
97	8.5
98	10.5
99	14.0
100	24.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07731190073760967
2	0.013449225620656386
3	0.017763128178225417
4	0.010810132291320038
5	0.008881564089112708
6	0.007189837595948382
7	0.018828915868918942
8	0.009152240328019
9	0.009135323063087357
10-11	0.012806369553253942
12-13	0.01487873450738024
14-15	0.009964269044737876
16-17	0.012679490066266617
18-19	0.008221790756778622
20-21	0.01253569331434765
22-23	0.013195466646681739
24-25	0.01492102766970935
26-27	0.005193600334014479
28-29	0.01102159810296558
30-31	0.010285697078439097
32-33	0.011867461349547742
34-35	0.008653181012535524
36-37	0.009558254686378439
38-39	0.018549780997546828
40-41	0.012772535023390657
42-43	0.008001866312667258
44-45	0.01979319997002261
46-47	0.01807609757946082
48-49	0.016968016726438183
50-51	0.010260321181041632
52-53	0.010124983061588488
54-55	0.011410695196393374
56-57	0.018972712620837908
58-59	0.0022330789709769094
60-61	0.0067415300752598365
62-63	0.0152932074982055
64-65	0.0235065396225183
66-67	0.012721783228595728
68-69	0.013829864081618359
70-71	0.024868379449515584
72-73	0.019378726979197347
74-75	0.01965786185056946
76-77	0.009118405798155713
78-79	0.010843966821183326
80-81	0.019015005783167015
82-83	0.012036633998864175
84-85	0.010937011778307363
86-87	0.016274408864240808
88-89	0.014853358609982778
90-91	0.014946403567106815
92-93	0.008077994004859654
94-95	0.015217079806013105
96-97	0.013288511603805776
98-99	0.009921975882408769
100	0.006868409562247161
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5911121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.68666426385615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63762837021888	49.444815722561636
2	13.838179673934164	17.626150058335273
3	4.128306096534845	7.887541330453356
4	1.6317177620632304	4.156746451435668
5	0.8105630511492841	2.5811028451615656
6	0.4653256898470101	1.7781024589580277
7	0.2944220024405756	1.3125528654927623
8	0.2052949270591401	1.0459639275750634
9	0.14705930593610844	0.8429144979625782
>10	0.7761439829748762	8.78681182805733
>50	0.04449558413872769	1.9265305699006992
>100	0.02011827518887252	2.223548668294605
>500	6.388143179085709E-4	0.24259392035703886
>1k	1.0646419646621724E-4	0.14462485545436343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.6917264931643254E-5	0.0	0.0	0.0	0.0
3	1.6917264931643254E-5	0.0	0.0	0.0	0.0
4	1.6917264931643254E-5	0.0	0.0	1.6917264931643254E-5	0.0
5	1.6917264931643254E-5	0.0	0.0	1.6917264931643254E-5	0.0
6	1.6917264931643254E-5	0.0	0.0	1.6917264931643254E-5	0.0
7	1.6917264931643254E-5	0.0	0.0	3.383452986328651E-5	0.0
8	1.6917264931643254E-5	0.0	0.0	3.383452986328651E-5	0.0
9	6.766905972657302E-5	0.0	0.0	3.383452986328651E-5	0.0
10-11	6.766905972657302E-5	0.0	0.0	4.229316232910813E-5	0.0
12-13	6.766905972657302E-5	0.0	0.0	8.458632465821626E-5	0.0
14-15	6.766905972657302E-5	0.0	0.0	1.4379675191896765E-4	0.0
16-17	6.766905972657302E-5	0.0	0.0	1.5225538438478928E-4	0.0
18-19	6.766905972657302E-5	0.0	0.0	1.6917264931643253E-4	0.0
20-21	6.766905972657302E-5	0.0	0.0	2.537589739746488E-4	8.458632465821627E-6
22-23	6.766905972657302E-5	0.0	0.0	4.31390255756903E-4	1.6917264931643254E-5
24-25	6.766905972657302E-5	0.0	0.0	7.274423920606599E-4	1.6917264931643254E-5
26-27	6.766905972657302E-5	0.0	0.0	0.0013872157243947467	1.6917264931643254E-5
28-29	6.766905972657302E-5	0.0	0.0	0.003493415208384332	1.6917264931643254E-5
30-31	6.766905972657302E-5	0.0	0.0	0.009228368020211396	1.6917264931643254E-5
32-33	6.766905972657302E-5	0.0	0.0	0.022804473127855107	1.6917264931643254E-5
34-35	6.766905972657302E-5	0.0	0.0	0.04716533462942139	1.6917264931643254E-5
36-37	6.766905972657302E-5	0.0	0.0	0.08431564841930997	1.6917264931643254E-5
38-39	6.766905972657302E-5	0.0	0.0	0.13241143261997174	1.6917264931643254E-5
40-41	6.766905972657302E-5	8.458632465821627E-6	0.0	0.1829348443383243	1.6917264931643254E-5
42-43	6.766905972657302E-5	1.6917264931643254E-5	0.0	0.23707855075204856	2.537589739746488E-5
44-45	6.766905972657302E-5	1.6917264931643254E-5	0.0	0.30657467509123904	3.383452986328651E-5
46-47	6.766905972657302E-5	1.6917264931643254E-5	0.0	0.39342791325029547	3.383452986328651E-5
48-49	6.766905972657302E-5	1.6917264931643254E-5	0.0	0.48315708644773137	3.383452986328651E-5
50-51	8.458632465821626E-5	1.6917264931643254E-5	0.0	0.5750178350265542	3.383452986328651E-5
52-53	8.458632465821626E-5	1.6917264931643254E-5	0.0	0.6670223803572961	3.383452986328651E-5
54-55	8.458632465821626E-5	1.6917264931643254E-5	0.0	0.773956411990213	3.383452986328651E-5
56-57	8.458632465821626E-5	1.6917264931643254E-5	0.0	0.9056573194830557	3.383452986328651E-5
58-59	8.458632465821626E-5	1.6917264931643254E-5	0.0	1.0550875206242605	3.383452986328651E-5
60-61	8.458632465821626E-5	1.6917264931643254E-5	0.0	1.2070553115052118	3.383452986328651E-5
62-63	8.458632465821626E-5	1.6917264931643254E-5	0.0	1.360562573494943	3.383452986328651E-5
64-65	8.458632465821626E-5	1.6917264931643254E-5	0.0	1.5304372893060385	4.229316232910813E-5
66-67	8.458632465821626E-5	1.6917264931643254E-5	0.0	1.7251550086692524	5.075179479492976E-5
68-69	8.458632465821626E-5	1.6917264931643254E-5	0.0	1.945155580472807	5.075179479492976E-5
70-71	8.458632465821626E-5	1.6917264931643254E-5	0.0	2.182454055668967	5.075179479492976E-5
72-73	8.458632465821626E-5	1.6917264931643254E-5	0.0	2.4288624780308172	5.075179479492976E-5
74-75	8.458632465821626E-5	1.6917264931643254E-5	0.0	2.686250205333303	5.075179479492976E-5
76-77	9.30449571240379E-5	1.6917264931643254E-5	0.0	2.9648944760223994	5.075179479492976E-5
78-79	1.0150358958985953E-4	1.6917264931643254E-5	0.0	3.276510496063268	5.075179479492976E-5
80-81	1.0150358958985953E-4	1.6917264931643254E-5	0.0	3.6211405586182384	5.075179479492976E-5
82-83	1.0150358958985953E-4	1.6917264931643254E-5	0.0	3.975717972953015	5.075179479492976E-5
84-85	1.0150358958985953E-4	1.6917264931643254E-5	0.0	4.330802905235741	5.075179479492976E-5
86-87	1.0150358958985953E-4	1.6917264931643254E-5	0.0	4.7075334779985045	5.075179479492976E-5
88	1.0150358958985953E-4	1.6917264931643254E-5	0.0	5.012974696339324	5.075179479492976E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6370	0.0	44.964096	1
GTATCAA	11075	0.0	33.675762	1
ATCAACG	11380	0.0	31.183727	3
TCAACGC	11420	0.0	30.90699	4
CAACGCA	12340	0.0	28.679157	5
AACGCAG	12795	0.0	27.806467	6
TATCAAC	13005	0.0	27.720728	2
GTACATG	18070	0.0	24.101318	1
ACGCAGA	14690	0.0	23.611967	7
CGCAGAG	14975	0.0	22.909758	8
TACATGG	18260	0.0	22.883402	2
ACATGGG	18545	0.0	21.720875	3
GTGGTAT	3555	0.0	20.770552	1
GCAGAGT	17445	0.0	18.939125	9
TGGTATC	3665	0.0	18.723995	2
GTACCGT	355	9.094947E-11	18.534885	6
CATGGGG	11900	0.0	18.404408	4
CAGAGTA	16865	0.0	17.041061	10-11
AGAGTAC	15785	0.0	16.375881	10-11
GTATAGG	2025	0.0	16.025517	1
>>END_MODULE