FastQCFastQC Report
Wed 25 May 2016
SRR2049467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911121
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205010.3468208483636184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198160.33523252188544267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113180.19146960449633835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116850.058.1251531
GTATCAA211950.040.5280721
GTGGTAT44800.034.460561
ATCAACG264100.032.1803173
TCAACGC265250.032.0762444
TGGTATC47750.031.403472
TATCAAC274200.031.2178362
CAACGCA276200.030.7705365
AACGCAG287000.029.656746
ACGCAGA322500.026.2464687
CGCAGAG324300.026.0138328
GCAGAGT355450.023.4960989
GTACATG169700.021.77281
GAGTACT263850.021.31289512-13
CAGAGTA350750.021.07074210-11
TACATGG167850.021.031922
TATACCG3850.020.7562315
AGAGTAC336950.020.44123610-11
ACATGGG172250.019.648693
GTACTTT285050.019.52993814-15
Produced by FastQC (version 0.11.5)