##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049467_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5911121
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85869533037811	31.0	31.0	34.0	30.0	34.0
2	32.126157119774746	33.0	31.0	34.0	30.0	34.0
3	32.115745389072565	34.0	31.0	34.0	30.0	34.0
4	35.80991659619216	37.0	35.0	37.0	33.0	37.0
5	35.88049863976731	37.0	35.0	37.0	35.0	37.0
6	35.96337429059564	37.0	35.0	37.0	35.0	37.0
7	35.93252109033126	37.0	35.0	37.0	35.0	37.0
8	35.9368287335008	37.0	35.0	37.0	35.0	37.0
9	37.65757747134596	39.0	37.0	39.0	35.0	39.0
10-11	37.6309066419043	39.0	37.0	39.0	35.0	39.0
12-13	37.61717498254561	39.0	37.0	39.0	35.0	39.0
14-15	38.90547672767991	40.0	38.0	41.0	35.0	41.0
16-17	38.91316917721698	40.0	38.0	41.0	35.5	41.0
18-19	38.99948960611701	40.0	38.5	41.0	36.0	41.0
20-21	39.02799739677127	40.0	39.0	41.0	35.5	41.0
22-23	38.99917266115852	40.0	39.0	41.0	35.0	41.0
24-25	38.980596996745625	40.0	38.5	41.0	35.0	41.0
26-27	38.88262945387178	40.0	38.0	41.0	35.0	41.0
28-29	38.802421655723165	40.0	38.0	41.0	35.0	41.0
30-31	38.684910527123364	40.0	38.0	41.0	35.0	41.0
32-33	38.54352854560074	40.0	38.0	41.0	34.5	41.0
34-35	38.42726269687255	40.0	38.0	41.0	34.0	41.0
36-37	38.2922562403984	40.0	38.0	41.0	34.0	41.0
38-39	38.18653991349526	40.0	38.0	41.0	34.0	41.0
40-41	38.09232842636786	40.0	38.0	41.0	34.0	41.0
42-43	37.945146022218125	40.0	38.0	41.0	33.0	41.0
44-45	37.75941653029942	40.0	37.5	41.0	33.0	41.0
46-47	37.49040435815812	40.0	37.0	41.0	33.0	41.0
48-49	37.23023390994703	40.0	37.0	41.0	32.0	41.0
50-51	37.066926814727694	40.0	37.0	41.0	32.0	41.0
52-53	36.95326656314428	40.0	37.0	41.0	31.5	41.0
54-55	36.810572816898855	40.0	36.0	41.0	31.0	41.0
56-57	36.663661850264944	40.0	36.0	41.0	31.0	41.0
58-59	36.488722866610246	39.0	35.5	41.0	31.0	41.0
60-61	36.30518196125574	39.0	35.0	41.0	31.0	41.0
62-63	36.08810748418109	39.0	35.0	41.0	30.5	41.0
64-65	35.870630122442094	38.5	35.0	40.5	30.0	41.0
66-67	35.60627138236555	38.0	35.0	40.0	30.0	41.0
68-69	35.31368533988731	37.0	35.0	40.0	29.5	41.0
70-71	35.02822797909229	37.0	35.0	40.0	29.0	41.0
72-73	34.694457785587545	36.5	34.5	39.0	29.0	41.0
74-75	34.369780199051924	36.0	34.0	39.0	29.0	41.0
76-77	34.047704318690144	35.5	34.0	38.5	29.0	40.0
78-79	33.734883451040844	35.0	34.0	37.0	29.0	39.5
80-81	33.42233182166293	35.0	34.0	37.0	29.0	39.0
82-83	33.14510344146229	35.0	34.0	36.5	28.5	39.0
84-85	32.85903655161178	35.0	34.0	36.0	28.0	37.5
86-87	32.61085926002868	35.0	34.0	36.0	27.5	37.0
88-89	32.39103378191717	35.0	34.0	35.5	27.0	37.0
90-91	32.19815175497169	35.0	33.0	35.0	27.0	36.0
92-93	32.033803740441115	35.0	33.0	35.0	27.0	36.0
94-95	31.87299600194278	35.0	33.0	35.0	26.0	36.0
96-97	31.729412830493573	35.0	33.0	35.0	26.0	36.0
98-99	31.593347268648365	35.0	33.0	35.0	25.5	35.5
100	31.48394255505851	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28057376726038896
1101	2	0.3350833285255561
1101	3	0.4403280510501162
1101	4	0.26399199365482673
1101	5	0.23369518240805576
1101	6	0.19922295249728705
1101	7	0.23582077744503493
1101	8	0.2354802121102395
1101	9	0.313612970727398
1101	10-11	0.2904025592593058
1101	12-13	0.2878191817002218
1101	14-15	0.39173030475335935
1101	16-17	0.3736221048170094
1101	18-19	0.3768325072175642
1101	20-21	0.3892146258077531
1101	22-23	0.383877129686212
1101	24-25	0.3671383134156727
1101	26-27	0.4017256098196711
1101	28-29	0.39086046634862015
1101	30-31	0.3982883809051714
1101	32-33	0.42269826263402877
1101	34-35	0.42583252088628853
1101	36-37	0.4599123426108491
1101	38-39	0.42466885225094586
1101	40-41	0.4526065396893131
1101	42-43	0.4737317802201346
1101	44-45	0.456428343063223
1101	46-47	0.4623845917845344
1101	48-49	0.43920021129323317
1101	50-51	0.4186918035741982
1101	52-53	0.41978560175645185
1101	54-55	0.37745832002936197
1101	56-57	0.4383662363425245
1101	58-59	0.422098316648686
1101	60-61	0.3198208609478499
1101	62-63	0.35778420808002664
1101	64-65	0.35767979043559706
1101	66-67	0.3846830133481234
1101	68-69	0.39584979272520116
1101	70-71	0.3684035743902143
1101	72-73	0.41292960444300775
1101	74-75	0.411475264423224
1101	76-77	0.39362672503163054
1101	78-79	0.4041915426912297
1101	80-81	0.3725189151610593
1101	82-83	0.3683369182847045
1101	84-85	0.4129740208198456
1101	86-87	0.3774294234301152
1101	88-89	0.3731345771900152
1101	90-91	0.359745872763483
1101	92-93	0.36687232932894887
1101	94-95	0.34787372855357646
1101	96-97	0.3504961000692113
1101	98-99	0.4485294039332821
1101	100	0.44799051291813186
1102	1	0.26049585838550726
1102	2	0.28643233031321813
1102	3	0.32001756248229185
1102	4	0.23031505823404785
1102	5	0.22956898692820715
1102	6	0.2013873263129824
1102	7	0.22261933159773406
1102	8	0.22050422661975944
1102	9	0.27374048634904824
1102	10-11	0.23037054883013752
1102	12-13	0.23393834489483822
1102	14-15	0.35733816713181454
1102	16-17	0.3388031001698266
1102	18-19	0.342464570523731
1102	20-21	0.2896471606154307
1102	22-23	0.2670305782807887
1102	24-25	0.26853689297029604
1102	26-27	0.28075875642456793
1102	28-29	0.25847452293849216
1102	30-31	0.2770452858325001
1102	32-33	0.278932742064967
1102	34-35	0.32997342640614846
1102	36-37	0.2872209731827553
1102	38-39	0.3690841698192173
1102	40-41	0.3937293607489636
1102	42-43	0.3445524527668482
1102	44-45	0.4008978723815133
1102	46-47	0.43359402848000883
1102	48-49	0.4030112557996972
1102	50-51	0.36589656716387964
1102	52-53	0.3850563091262984
1102	54-55	0.3561721899391017
1102	56-57	0.3757091706496922
1102	58-59	0.33033107378723514
1102	60-61	0.2838824275506724
1102	62-63	0.3388716498076292
1102	64-65	0.2935213894701647
1102	66-67	0.30917908650029347
1102	68-69	0.31453101130614414
1102	70-71	0.3171996081911459
1102	72-73	0.320765519310811
1102	74-75	0.33296529534077735
1102	76-77	0.3306149631857025
1102	78-79	0.3051676439894919
1102	80-81	0.31550373079958405
1102	82-83	0.2760224725238487
1102	84-85	0.24883632091280106
1102	86-87	0.21608596235916622
1102	88-89	0.19731090829815656
1102	90-91	0.224590824929237
1102	92-93	0.21805369127635998
1102	94-95	0.2253892248102467
1102	96-97	0.1756793263100711
1102	98-99	0.20912249970536578
1102	100	0.25556674918428257
1103	1	0.3173133492274367
1103	2	0.2935953977657846
1103	3	0.37694657639416107
1103	4	0.2244888732405954
1103	5	0.23556635258870529
1103	6	0.21062925050070191
1103	7	0.2302889964915522
1103	8	0.2136555235354578
1103	9	0.2763947026046125
1103	10-11	0.2562468812013634
1103	12-13	0.242616474715021
1103	14-15	0.33607136840550567
1103	16-17	0.3293665642606527
1103	18-19	0.3022518094410884
1103	20-21	0.33979248783434457
1103	22-23	0.32855103990701195
1103	24-25	0.32277616127397124
1103	26-27	0.29837326346799387
1103	28-29	0.3261810289959044
1103	30-31	0.3383989686583746
1103	32-33	0.300891754835078
1103	34-35	0.3298129086460051
1103	36-37	0.32228307176531246
1103	38-39	0.29207507036787206
1103	40-41	0.29730422879138985
1103	42-43	0.2734684299353205
1103	44-45	0.2829772906964294
1103	46-47	0.3268392191262208
1103	48-49	0.3151642820748535
1103	50-51	0.26993721285264627
1103	52-53	0.2541012123224178
1103	54-55	0.2833752121240707
1103	56-57	0.29378365089039704
1103	58-59	0.25016129734300563
1103	60-61	0.18436417768475621
1103	62-63	0.18075196376297242
1103	64-65	0.17632941241630107
1103	66-67	0.18162627722350067
1103	68-69	0.15206803619982168
1103	70-71	0.22036983258682596
1103	72-73	0.2021036195285717
1103	74-75	0.18630951923863392
1103	76-77	0.18021214590352486
1103	78-79	0.1906830336810046
1103	80-81	0.12526806633972143
1103	82-83	0.16369257596387854
1103	84-85	0.16452125738592827
1103	86-87	0.12409024294213822
1103	88-89	0.13881492553466046
1103	90-91	0.11462570138387917
1103	92-93	0.11647456124269695
1103	94-95	0.1691458084717361
1103	96-97	0.17457732855157815
1103	98-99	0.22759731195071353
1103	100	0.1819205391860379
1104	1	0.29864139771254017
1104	2	0.29246551792543585
1104	3	0.3863597713139626
1104	4	0.21849467323427518
1104	5	0.2176951582586355
1104	6	0.17398409795796965
1104	7	0.18948072274659467
1104	8	0.15983892760256424
1104	9	0.2187099742538834
1104	10-11	0.24360888452918772
1104	12-13	0.23571464020044886
1104	14-15	0.3297588466851451
1104	16-17	0.29110841846834035
1104	18-19	0.22451553802350332
1104	20-21	0.31887442755443374
1104	22-23	0.3091307891619408
1104	24-25	0.2948758682465993
1104	26-27	0.2908865918692669
1104	28-29	0.3103909345320943
1104	30-31	0.30478603069698096
1104	32-33	0.3518012257008394
1104	34-35	0.36302382253334287
1104	36-37	0.3164260785999886
1104	38-39	0.33492507089442825
1104	40-41	0.3170046589861073
1104	42-43	0.3283171246076506
1104	44-45	0.37113455530040085
1104	46-47	0.3212961706937705
1104	48-49	0.3199949620141993
1104	50-51	0.38145420212147485
1104	52-53	0.380044718330403
1104	54-55	0.33975591602163036
1104	56-57	0.3780333902461095
1104	58-59	0.3736942780605972
1104	60-61	0.33309843371643666
1104	62-63	0.3396114672750912
1104	64-65	0.2862292420760397
1104	66-67	0.3723357299231367
1104	68-69	0.3850101442066034
1104	70-71	0.36263921504414753
1104	72-73	0.38198116278499583
1104	74-75	0.32847492700214787
1104	76-77	0.3437179695921344
1104	78-79	0.34065606752790956
1104	80-81	0.29330296634709185
1104	82-83	0.2988522535021332
1104	84-85	0.33704967156287324
1104	86-87	0.35317324235239056
1104	88-89	0.35058679990937947
1104	90-91	0.3581920722148908
1104	92-93	0.3952882413821257
1104	94-95	0.343676767221492
1104	96-97	0.3237566676208381
1104	98-99	0.3684479667207654
1104	100	0.3801471970417012
1105	1	0.23778814449950758
1105	2	0.24498169117244117
1105	3	0.38731457776901834
1105	4	0.23173006009529473
1105	5	0.21345882287228335
1105	6	0.1418695730598145
1105	7	0.18562781537657713
1105	8	0.15289078879744977
1105	9	0.20145272553681082
1105	10-11	0.18284912336430637
1105	12-13	0.20297942880390707
1105	14-15	0.2888607864575974
1105	16-17	0.2806648430109959
1105	18-19	0.2633611153016133
1105	20-21	0.2584619398242296
1105	22-23	0.22012365742117623
1105	24-25	0.24858741864050415
1105	26-27	0.27967546742173255
1105	28-29	0.3287265713664098
1105	30-31	0.3221855009977048
1105	32-33	0.32221309676242527
1105	34-35	0.33856139863151924
1105	36-37	0.35093100813447364
1105	38-39	0.35869444827527985
1105	40-41	0.3834935195350795
1105	42-43	0.36426847898993486
1105	44-45	0.3675455058092538
1105	46-47	0.3526975914474022
1105	48-49	0.36677684823184364
1105	50-51	0.4000787704534403
1105	52-53	0.44111734027821115
1105	54-55	0.4047691140014962
1105	56-57	0.4056116944455539
1105	58-59	0.39374302666832506
1105	60-61	0.34502533877743957
1105	62-63	0.3450981650337184
1105	64-65	0.38623758935328567
1105	66-67	0.4148461370007226
1105	68-69	0.4644675076113245
1105	70-71	0.43170157359180905
1105	72-73	0.5032377363452909
1105	74-75	0.46505078868528926
1105	76-77	0.40250442297199385
1105	78-79	0.3710612505958366
1105	80-81	0.3300437582767515
1105	82-83	0.3319415598325861
1105	84-85	0.33335912604637485
1105	86-87	0.3383937299910471
1105	88-89	0.3254661680847022
1105	90-91	0.3608891455183425
1105	92-93	0.44085872978367746
1105	94-95	0.40477418306211277
1105	96-97	0.43188525637789255
1105	98-99	0.42269858720382913
1105	100	0.40455903504476254
1106	1	0.19933985797834808
1106	2	0.18945425671689975
1106	3	0.2788284186422558
1106	4	0.14138684501648413
1106	5	0.18957881584922376
1106	6	0.16846665205937938
1106	7	0.13244734493031984
1106	8	0.1149202065262287
1106	9	0.16254690134087468
1106	10-11	0.17553156224635558
1106	12-13	0.16835825377633284
1106	14-15	0.24724673093583505
1106	16-17	0.23630168515529704
1106	18-19	0.21847069609403036
1106	20-21	0.23338882207912803
1106	22-23	0.1996334464325571
1106	24-25	0.1764772542414974
1106	26-27	0.22363680843097455
1106	28-29	0.21287698483683926
1106	30-31	0.24607353879365235
1106	32-33	0.23277985047550231
1106	34-35	0.2428942757130912
1106	36-37	0.24677468441086603
1106	38-39	0.17095391457199582
1106	40-41	0.1625423953505205
1106	42-43	0.22432879942951445
1106	44-45	0.2505970140366216
1106	46-47	0.2802061044375961
1106	48-49	0.27211242820325054
1106	50-51	0.25832135419851454
1106	52-53	0.271907929374656
1106	54-55	0.3200838411654132
1106	56-57	0.32419654119937036
1106	58-59	0.3124943311279722
1106	60-61	0.29271104189972874
1106	62-63	0.2980147636953845
1106	64-65	0.22233081926955833
1106	66-67	0.2565175949291074
1106	68-69	0.2844807191291494
1106	70-71	0.3119782921401111
1106	72-73	0.3520950235143516
1106	74-75	0.32392221471314997
1106	76-77	0.33044254212665436
1106	78-79	0.2782099738327801
1106	80-81	0.2940426318665672
1106	82-83	0.28184072695674445
1106	84-85	0.2568324074980666
1106	86-87	0.22088705313600343
1106	88-89	0.21301260313869363
1106	90-91	0.24609692553822526
1106	92-93	0.28720686100613335
1106	94-95	0.24863169897529147
1106	96-97	0.23120607802672666
1106	98-99	0.2501222423121199
1106	100	0.21698906407671714
1107	1	0.09473478795094081
1107	2	0.15518298277490317
1107	3	0.20578969207812037
1107	4	0.1383142797069823
1107	5	0.13030464970676547
1107	6	0.11397202793698114
1107	7	0.09241495228518204
1107	8	0.08878541552386565
1107	9	0.10742524815909604
1107	10-11	0.10439552398254648
1107	12-13	0.1553851620863469
1107	14-15	0.14292162591281254
1107	16-17	0.15920755126087727
1107	18-19	0.15343467012207412
1107	20-21	0.166721576972364
1107	22-23	0.1683081317249986
1107	24-25	0.18087582115617096
1107	26-27	0.16435374877210762
1107	28-29	0.16703569795224382
1107	30-31	0.169586441622954
1107	32-33	0.15790054561588107
1107	34-35	0.1611756036669405
1107	36-37	0.17203585011226608
1107	38-39	0.1023193404846694
1107	40-41	0.1797392746662254
1107	42-43	0.1827893895830215
1107	44-45	0.22953563069714278
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2216	74-75	-0.7415066471239911
2216	76-77	-0.6865415826918593
2216	78-79	-0.6184904146617782
2216	80-81	-0.6220399115733031
2216	82-83	-0.668103221589611
2216	84-85	-0.7357734564162826
2216	86-87	-0.6774959874905058
2216	88-89	-0.712509658838755
2216	90-91	-0.6056405387758659
2216	92-93	-0.6396325191762102
2216	94-95	-0.6386618514693154
2216	96-97	-0.6614781044349662
2216	98-99	-0.7126759531266806
2216	100	-0.7877006234790649
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	392.0
3	52.0
4	47.0
5	152.0
6	502.0
7	1193.0
8	2314.0
9	3806.0
10	4972.0
11	6566.0
12	9500.0
13	13689.0
14	16590.0
15	21893.0
16	27968.0
17	40146.0
18	41446.0
19	21450.0
20	14464.0
21	13590.0
22	14045.0
23	15535.0
24	18154.0
25	21596.0
26	28247.0
27	36260.0
28	47139.0
29	62058.0
30	80954.0
31	104971.0
32	137458.0
33	181621.0
34	248854.0
35	368700.0
36	613802.0
37	1185218.0
38	1765348.0
39	737932.0
40	2497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06060505968428	20.942437465370993	15.37489779626126	22.622059678683474
2	15.39254567788411	21.081382025507516	40.039241287735436	23.486831008872937
3	17.660034365731985	26.046142516791654	31.28684051637583	25.006982601100535
4	12.572522200103839	18.192928211078744	38.68185070141518	30.552698887402236
5	12.407090384707141	37.91400930644294	35.77803142100402	13.90086888784589
6	29.376734973599728	38.97736348467708	17.71285712183137	13.933044419891822
7	25.584605694926566	33.52191572461467	22.972055554267964	17.9214230261908
8	24.486235351974695	36.077911448606784	20.66951429348173	18.76633890593679
9	24.58400022601466	16.89599992962418	22.144513705606773	36.37548613875439
10-11	23.280930977389907	27.40958948395744	29.060012474791158	20.24946706386149
12-13	24.28839639723159	25.12325496297572	29.27660421771099	21.3117444220817
14-15	21.576947925782605	26.96333741095809	27.335855584752878	24.12385907850643
16-17	20.191804566341983	30.16631701499597	28.067891014242473	21.57398740441957
18-19	20.04760180006466	28.321091718474378	31.341136478173937	20.290170003287024
20-21	21.148712739935455	27.5254473728418	31.17476363620369	20.151076251019052
22-23	20.88345002580729	27.340389411754558	31.25948529898136	20.516675263456797
24-25	20.66251054580003	27.52161561233478	31.22523629612725	20.59063754573794
26-27	20.46680316643831	27.505417331162736	31.265507845297023	20.762271657101927
28-29	20.53680680872545	27.45298226850711	31.438698345034723	20.57151257773272
30-31	20.825119296322985	27.767127419655257	31.012214096108	20.395539187913762
32-33	20.469839815493543	27.597303455638954	31.337761483820074	20.59509524504743
34-35	20.73538166449308	27.3923254150947	31.22276637544723	20.649526544964992
36-37	20.964272259018212	27.656395462045186	30.7202305620203	20.6591017169163
38-39	20.81087495925054	27.79544692115083	30.813926833844206	20.579751285754426
40-41	20.80931011224436	27.520253772507786	30.85160158284698	20.818834532400878
42-43	20.79113251107531	27.631222571826903	30.658338748267884	20.919306168829905
44-45	20.964492183462326	27.768556928541983	30.362405032818646	20.904545855177048
46-47	21.075122637482803	27.849235365000986	29.978789133228705	21.096852864287502
48-49	21.120900756387833	27.872445852487203	29.69242213109832	21.314231260026652
50-51	21.17845329168528	27.971471062764575	29.40285776589584	21.4472178796543
52-53	21.159818924363076	28.075656038846102	29.33144153198691	21.43308350480391
54-55	21.261517062499653	28.317302251129696	29.18657053374478	21.234610152625873
56-57	21.176211754081837	28.035409865573722	29.239064806827674	21.549313573516766
58-59	21.07705966431748	27.722085201774753	29.512075628294532	21.688779505613233
60-61	21.17641476126102	28.025284882512132	29.288488596325468	21.50981175990138
62-63	21.012486464073397	28.007496378436507	29.403737463672286	21.576279693817803
64-65	21.080536162260934	27.808642387797512	29.455648091115034	21.655173358826524
66-67	20.998478968709996	27.88520147024566	29.34882402170417	21.76749553934017
68-69	21.294691819030604	27.989445656754448	29.187407938358902	21.52845458585605
70-71	21.30336191730807	27.797959134993178	29.235106166833667	21.663572780865085
72-73	21.13238757927642	27.89497964937615	29.254831697743967	21.717801073603468
74-75	21.22759974187635	27.934111646006876	29.072762093075248	21.765526519041526
76-77	21.300274516458046	27.98009040924725	28.891863320003093	21.827771754291614
78-79	21.31802072737134	28.0259869490068	28.992241911475	21.663750412146868
80-81	21.163327644612384	27.922571928936552	29.020123455620535	21.89397697083053
82-83	21.09007371149111	27.961872999151677	29.10336365404228	21.84468963531493
84-85	21.071846097016962	27.856301884902816	28.99199565292453	22.079856365155692
86-87	21.161198521527172	27.996373601899275	28.86894392489925	21.973483951674304
88-89	21.024873325002666	27.893131074015688	28.86570103000436	22.216294570977286
90-91	20.924975389467285	27.825330603322197	28.912668685133546	22.337025322076972
92-93	20.943276783857915	27.970708363086644	28.779296331465492	22.30671852158995
94-95	20.793339045181828	27.762197403209505	28.9115257563117	22.53293779529697
96-97	20.839750296497744	27.816745488840006	28.835043212415712	22.50846100224654
98-99	20.755239665819893	27.86791007507278	28.757411525234712	22.619438733872617
100	20.8171181211805	27.777715747753895	28.74717735195858	22.657988779107026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1456.0
1	1015.5
2	1126.5
3	1662.5
4	1108.5
5	703.5
6	1677.0
7	4447.5
8	7483.0
9	8658.5
10	6633.5
11	3980.0
12	3498.0
13	3763.0
14	4330.5
15	4953.0
16	5574.0
17	6333.5
18	7247.0
19	8384.5
20	9953.0
21	12180.0
22	15369.0
23	20052.0
24	26440.0
25	35132.5
26	46340.5
27	59748.5
28	76152.5
29	94578.0
30	112340.0
31	128911.0
32	144247.0
33	157800.0
34	170050.5
35	181456.5
36	191435.5
37	200075.0
38	208459.5
39	213220.5
40	216622.5
41	218531.0
42	217060.0
43	215832.5
44	212214.5
45	207601.5
46	202942.5
47	196371.0
48	189867.0
49	184119.5
50	177651.5
51	168317.5
52	154486.5
53	141453.0
54	133540.0
55	126477.5
56	115982.0
57	103513.5
58	92900.5
59	84104.0
60	73241.5
61	60947.5
62	50420.5
63	41235.5
64	33013.0
65	26107.5
66	20494.5
67	15750.5
68	12060.5
69	9169.0
70	6670.0
71	4790.0
72	3436.5
73	2419.0
74	1693.5
75	1147.0
76	703.5
77	418.0
78	263.0
79	148.5
80	71.5
81	30.0
82	16.5
83	11.0
84	7.5
85	4.0
86	3.5
87	2.5
88	0.5
89	0.0
90	0.5
91	1.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1264058035692384
2	0.0
3	0.0
4	0.0
5	0.013652232799836106
6	1.3533811945314603E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	8.458632465821627E-6
76-77	0.0
78-79	0.0
80-81	1.5225538438478928E-4
82-83	5.836456401416923E-4
84-85	3.4680393109868667E-4
86-87	3.6372119603032994E-4
88-89	4.99059315483476E-4
90-91	5.751870076758706E-4
92-93	2.3684170904300555E-4
94-95	2.791348713721137E-4
96-97	5.159765804151192E-4
98-99	9.642841011036654E-4
100	8.458632465821626E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5911121.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50916727292031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94369323096151	45.952896370355965
2	14.697074995708107	17.786155386759127
3	4.475927882033609	8.125040067464989
4	1.8257508588570077	4.418986564690264
5	0.9079242664441859	2.7468870654707356
6	0.513975050604218	1.8660121386670985
7	0.3268975294251072	1.384620810236174
8	0.22121838043157802	1.07085919883031
9	0.1631302520379438	0.888378813705383
>10	0.8457313595201474	9.107219910476624
>50	0.051666415185948146	2.13556886235365
>100	0.0252777291932763	2.683367297477578
>500	0.0012012715211405288	0.4539088212617588
>1k	4.1903532329374995E-4	0.4107418474548279
>5k	2.793568819705638E-5	0.0965367582852903
>10k+	8.380706459116914E-5	0.8728200865104122
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20501	0.3468208483636184	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19816	0.33523252188544267	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11318	0.19146960449633835	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.6917264931643254E-5	0.0
3	0.0	0.0	0.0	1.6917264931643254E-5	0.0
4	0.0	0.0	0.0	3.383452986328651E-5	0.0
5	0.0	0.0	0.0	5.075179479492976E-5	0.0
6	0.0	0.0	0.0	6.766905972657302E-5	0.0
7	0.0	0.0	0.0	1.0150358958985953E-4	0.0
8	0.0	0.0	0.0	1.1842085452150277E-4	0.0
9	5.075179479492976E-5	0.0	0.0	1.3533811945314603E-4	0.0
10-11	5.075179479492976E-5	0.0	0.0	1.3533811945314603E-4	0.0
12-13	5.075179479492976E-5	0.0	0.0	1.4379675191896765E-4	0.0
14-15	5.075179479492976E-5	0.0	0.0	1.9454854671389741E-4	0.0
16-17	5.075179479492976E-5	0.0	0.0	2.199244441113623E-4	0.0
18-19	5.075179479492976E-5	0.0	0.0	2.283830765771839E-4	0.0
20-21	5.075179479492976E-5	0.0	0.0	3.1296940123540023E-4	0.0
22-23	5.075179479492976E-5	0.0	0.0	5.075179479492976E-4	0.0
24-25	5.075179479492976E-5	0.0	0.0	8.289459816505194E-4	0.0
26-27	5.075179479492976E-5	0.0	0.0	0.001497177946450428	0.0
28-29	5.075179479492976E-5	0.0	0.0	0.003628753327837478	0.0
30-31	5.075179479492976E-5	0.0	0.0	0.00945675109678858	0.0
32-33	5.075179479492976E-5	0.0	0.0	0.02308360799922722	0.0
34-35	5.075179479492976E-5	0.0	0.0	0.04744446950079351	0.0
36-37	5.075179479492976E-5	0.0	0.0	0.08449327970109223	0.0
38-39	5.075179479492976E-5	0.0	0.0	0.13277515381600208	0.0
40-41	5.075179479492976E-5	0.0	0.0	0.1837807075849065	0.0
42-43	5.075179479492976E-5	0.0	0.0	0.23828813519466102	0.0
44-45	5.075179479492976E-5	0.0	0.0	0.30810568756755274	0.0
46-47	5.075179479492976E-5	0.0	0.0	0.3953903159823661	0.0
48-49	5.075179479492976E-5	0.0	0.0	0.48566084165761453	0.0
50-51	5.075179479492976E-5	0.0	0.0	0.578206739466169	0.0
52-53	5.075179479492976E-5	0.0	0.0	0.6709810203513005	0.0
54-55	5.075179479492976E-5	0.0	0.0	0.778321066342577	0.0
56-57	5.075179479492976E-5	0.0	0.0	0.9107747921248779	0.0
58-59	5.075179479492976E-5	0.0	0.0	1.0611692773671864	0.0
60-61	5.9210427260751394E-5	0.0	0.0	1.2142197732037627	0.0
62-63	6.766905972657302E-5	0.0	0.0	1.3683022222011698	0.0
64-65	6.766905972657302E-5	0.0	0.0	1.5390312598913134	0.0
66-67	6.766905972657302E-5	0.0	0.0	1.7343072489972715	0.0
68-69	6.766905972657302E-5	0.0	0.0	1.9548660905435704	0.0
70-71	6.766905972657302E-5	0.0	0.0	2.1927312941149406	0.0
72-73	6.766905972657302E-5	0.0	0.0	2.4406030598933777	0.0
74-75	6.766905972657302E-5	0.0	0.0	2.69939492018519	0.0
76-77	6.766905972657302E-5	0.0	0.0	2.9801538489907413	0.0
78-79	6.766905972657302E-5	0.0	0.0	3.294045241164916	0.0
80-81	6.766905972657302E-5	0.0	0.0	3.6412213520920993	0.0
82-83	6.766905972657302E-5	0.0	0.0	3.9977019587316853	0.0
84-85	8.458632465821626E-5	0.0	0.0	4.354800045541277	0.0
86-87	8.458632465821626E-5	0.0	0.0	4.734398094709954	0.0
88	8.458632465821626E-5	0.0	0.0	5.042664496294358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11685	0.0	58.125153	1
GTATCAA	21195	0.0	40.528072	1
GTGGTAT	4480	0.0	34.46056	1
ATCAACG	26410	0.0	32.180317	3
TCAACGC	26525	0.0	32.076244	4
TGGTATC	4775	0.0	31.40347	2
TATCAAC	27420	0.0	31.217836	2
CAACGCA	27620	0.0	30.770536	5
AACGCAG	28700	0.0	29.65674	6
ACGCAGA	32250	0.0	26.246468	7
CGCAGAG	32430	0.0	26.013832	8
GCAGAGT	35545	0.0	23.496098	9
GTACATG	16970	0.0	21.7728	1
GAGTACT	26385	0.0	21.312895	12-13
CAGAGTA	35075	0.0	21.070742	10-11
TACATGG	16785	0.0	21.03192	2
TATACCG	385	0.0	20.756231	5
AGAGTAC	33695	0.0	20.441236	10-11
ACATGGG	17225	0.0	19.64869	3
GTACTTT	28505	0.0	19.529938	14-15
>>END_MODULE