FastQCFastQC Report
Wed 25 May 2016
SRR2049466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7399773
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74950.044.739171
GTATCAA134050.035.5045281
TCAACGC139350.032.951994
ATCAACG138950.032.948493
CAACGCA155800.029.4725845
TATCAAC166150.028.007222
AACGCAG168250.027.3756896
CGCAGAG175650.025.6849638
ACGCAGA182000.024.9980897
GTACATG198900.023.0773051
TACATGG194950.022.278392
GTGGTAT44700.021.35791
ACATGGG196950.021.2884463
GCAGAGT204750.021.025729
TGGTATC39100.020.6769682
AGAGTAC188400.017.74915710-11
ATACCGC4550.017.5602846
CAGAGTA206900.017.42282710-11
TCTGTCG17500.016.9183648
GAGTACT97750.015.96205912-13
Produced by FastQC (version 0.11.5)