##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049466_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7399773
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271423326093924	31.0	31.0	34.0	28.0	34.0
2	31.49279173834116	33.0	31.0	34.0	29.0	34.0
3	31.559065122673356	33.0	31.0	34.0	28.0	34.0
4	35.15368809286447	37.0	35.0	37.0	33.0	37.0
5	35.17934171764458	37.0	35.0	37.0	33.0	37.0
6	35.19065949725755	37.0	35.0	37.0	32.0	37.0
7	35.11746022479338	37.0	35.0	37.0	32.0	37.0
8	35.09279433301535	37.0	35.0	37.0	32.0	37.0
9	36.68994656998262	39.0	37.0	39.0	33.0	39.0
10-11	36.64587994793894	39.0	37.0	39.0	33.0	39.0
12-13	36.58731402976821	39.0	37.0	39.0	32.0	39.0
14-15	37.858354438710485	40.0	38.0	41.0	33.0	41.0
16-17	37.85739008480395	40.0	38.0	41.0	33.0	41.0
18-19	37.88870618058148	40.0	38.0	41.0	33.0	41.0
20-21	37.956184399170084	40.0	38.0	41.0	33.0	41.0
22-23	37.91964442693039	40.0	38.0	41.0	33.0	41.0
24-25	37.9326550287421	40.0	38.0	41.0	33.0	41.0
26-27	37.946479033883875	40.0	38.0	41.0	33.0	41.0
28-29	37.93020623740755	40.0	38.0	41.0	33.0	41.0
30-31	37.87472622470986	40.0	38.0	41.0	33.0	41.0
32-33	37.82974146639363	40.0	38.0	41.0	33.0	41.0
34-35	37.763624505778765	40.0	38.0	41.0	33.0	41.0
36-37	37.65002635891669	40.0	38.0	41.0	32.5	41.0
38-39	37.54898217823709	40.0	38.0	41.0	32.0	41.0
40-41	37.4389602897278	40.0	38.0	41.0	32.0	41.0
42-43	37.34859981515649	40.0	38.0	41.0	32.0	41.0
44-45	37.206007873484765	40.0	37.0	41.0	31.0	41.0
46-47	37.103225869225994	40.0	37.0	41.0	31.0	41.0
48-49	37.018898349989925	40.0	37.0	41.0	31.0	41.0
50-51	36.9267552531679	40.0	37.0	41.0	31.0	41.0
52-53	36.83350550077685	40.0	37.0	41.0	31.0	41.0
54-55	36.708440448105634	40.0	36.5	41.0	30.0	41.0
56-57	36.57374219452407	40.0	36.0	41.0	30.0	41.0
58-59	36.41448413349977	39.5	36.0	41.0	30.0	41.0
60-61	36.24568841503651	39.0	35.5	41.0	30.0	41.0
62-63	36.01552865202757	39.0	35.0	41.0	29.5	41.0
64-65	35.79238511775969	39.0	35.0	40.5	29.0	41.0
66-67	35.580598418356885	38.5	35.0	40.0	29.0	41.0
68-69	35.30509294001316	38.0	35.0	40.0	29.0	41.0
70-71	34.993385134922384	37.0	34.5	40.0	28.5	41.0
72-73	34.64837894351624	37.0	34.0	39.5	28.0	41.0
74-75	34.33384010563567	36.0	34.0	39.0	28.0	41.0
76-77	33.98543083686486	36.0	34.0	39.0	28.0	40.5
78-79	33.603398644796265	35.5	34.0	38.5	27.5	39.5
80-81	33.23271396298238	35.0	34.0	37.0	26.5	39.0
82-83	32.89621742450748	35.0	33.5	37.0	26.0	39.0
84-85	32.56849223618076	35.0	33.0	36.5	26.0	38.5
86-87	32.25768736419347	35.0	33.0	36.0	26.0	37.0
88-89	31.98837437310577	35.0	33.0	36.0	26.0	37.0
90-91	31.737400187816572	35.0	33.0	35.0	25.0	36.5
92-93	31.50320753082561	35.0	33.0	35.0	25.0	36.0
94-95	31.275925288518984	35.0	33.0	35.0	24.0	36.0
96-97	31.06324619687658	35.0	33.0	35.0	23.0	36.0
98-99	30.8683568401355	35.0	33.0	35.0	20.0	36.0
100	30.726047812547762	35.0	32.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37372496878032635
1101	2	0.3553019543114537
1101	3	0.41749861400959887
1101	4	0.4299841349117699
1101	5	0.42607256680668826
1101	6	0.4001111887253259
1101	7	0.48839385932190993
1101	8	0.470268790813968
1101	9	0.5715816759410188
1101	10-11	0.613145401859235
1101	12-13	0.539573867554445
1101	14-15	0.7323311661224139
1101	16-17	0.6451225084252101
1101	18-19	0.6660180931396127
1101	20-21	0.6373700123892831
1101	22-23	0.5849425648500386
1101	24-25	0.6073122371452442
1101	26-27	0.6459640444714836
1101	28-29	0.6192692028128093
1101	30-31	0.5958576355752285
1101	32-33	0.6383456645874972
1101	34-35	0.6628935017727144
1101	36-37	0.6301835559102855
1101	38-39	0.6296531078764147
1101	40-41	0.6388625869992381
1101	42-43	0.5928216647053262
1101	44-45	0.585754502331902
1101	46-47	0.5553493276891786
1101	48-49	0.5298489210232304
1101	50-51	0.5628416907690195
1101	52-53	0.5735781172171599
1101	54-55	0.48820125597188024
1101	56-57	0.518527899828868
1101	58-59	0.5040977333714309
1101	60-61	0.5417294589393506
1101	62-63	0.5082544460605973
1101	64-65	0.5057514442707642
1101	66-67	0.5009440898647028
1101	68-69	0.5024887040435218
1101	70-71	0.47452995368986706
1101	72-73	0.4343226516946217
1101	74-75	0.4804891689226807
1101	76-77	0.4400908781434012
1101	78-79	0.4402541507312847
1101	80-81	0.49283752815984627
1101	82-83	0.4500317866483172
1101	84-85	0.4417051276155277
1101	86-87	0.39676269575542733
1101	88-89	0.37876228448499916
1101	90-91	0.3655389947839609
1101	92-93	0.3392597311792649
1101	94-95	0.3375088572525833
1101	96-97	0.4122545713151453
1101	98-99	0.48283732031016413
1101	100	0.48132906174350865
1102	1	0.2733842326005629
1102	2	0.3681967428346731
1102	3	0.333403560023676
1102	4	0.355802154391192
1102	5	0.32842057820658255
1102	6	0.30100743791854256
1102	7	0.3292846492349568
1102	8	0.39900242013613507
1102	9	0.4621416011780539
1102	10-11	0.47209950692016633
1102	12-13	0.46547546923848415
1102	14-15	0.6143507820245517
1102	16-17	0.5616825420143741
1102	18-19	0.5691992438287627
1102	20-21	0.5683659090734423
1102	22-23	0.566528303418238
1102	24-25	0.522389279153046
1102	26-27	0.5128337671890151
1102	28-29	0.5552059043344855
1102	30-31	0.4852217284100746
1102	32-33	0.4846556496005263
1102	34-35	0.53515663386802
1102	36-37	0.5181605071210882
1102	38-39	0.5717137360999516
1102	40-41	0.6202483950586526
1102	42-43	0.5777342302700887
1102	44-45	0.46865223928315203
1102	46-47	0.5162382735323945
1102	48-49	0.45943561496545726
1102	50-51	0.4906564888563878
1102	52-53	0.4585446765214485
1102	54-55	0.45346833093852723
1102	56-57	0.3842593522109681
1102	58-59	0.4408926566576028
1102	60-61	0.47079512501580467
1102	62-63	0.43159099349303887
1102	64-65	0.41525288384335823
1102	66-67	0.43926578836762076
1102	68-69	0.3847630255795025
1102	70-71	0.38919723713016907
1102	72-73	0.38483042205270834
1102	74-75	0.4372069486209327
1102	76-77	0.3791633579016107
1102	78-79	0.3893699869674663
1102	80-81	0.4107950958004878
1102	82-83	0.39014043570047363
1102	84-85	0.3251465005203116
1102	86-87	0.2874994745821695
1102	88-89	0.3018316241152519
1102	90-91	0.3490450924561159
1102	92-93	0.3486447703553317
1102	94-95	0.3482314982106267
1102	96-97	0.3492105642034957
1102	98-99	0.3568556787025301
1102	100	0.33793200381627386
1103	1	0.28578341924222883
1103	2	0.3060901435625958
1103	3	0.31448574007661634
1103	4	0.3572464135481752
1103	5	0.29808076934460104
1103	6	0.3007056159649011
1103	7	0.2830190256592431
1103	8	0.34525743883467896
1103	9	0.43794118210666966
1103	10-11	0.39291707443540247
1103	12-13	0.40251645828031
1103	14-15	0.48767422839515717
1103	16-17	0.49827840921032873
1103	18-19	0.445888547141962
1103	20-21	0.4200482566596122
1103	22-23	0.41739085825230404
1103	24-25	0.46260893118200386
1103	26-27	0.4890735547816689
1103	28-29	0.4246618175365455
1103	30-31	0.3886466420071386
1103	32-33	0.42534216943134595
1103	34-35	0.3856230250461934
1103	36-37	0.4048292083751406
1103	38-39	0.4165798733568522
1103	40-41	0.42690139156201923
1103	42-43	0.399527267751175
1103	44-45	0.40243367336044145
1103	46-47	0.3825702014216148
1103	48-49	0.38733476794557475
1103	50-51	0.3572635626669296
1103	52-53	0.3782037505726876
1103	54-55	0.2930792004610012
1103	56-57	0.28539821473660965
1103	58-59	0.30243730170104755
1103	60-61	0.30505894695395597
1103	62-63	0.3508717474878935
1103	64-65	0.3635873223478754
1103	66-67	0.3423248039132929
1103	68-69	0.2872013281318786
1103	70-71	0.33631488513523067
1103	72-73	0.36041313920097195
1103	74-75	0.37836662172856705
1103	76-77	0.345802156976724
1103	78-79	0.361500042083037
1103	80-81	0.3145412388721951
1103	82-83	0.27469361290379624
1103	84-85	0.26413178655112546
1103	86-87	0.3318869327063112
1103	88-89	0.27300499121737687
1103	90-91	0.2820417805172255
1103	92-93	0.16761022288271832
1103	94-95	0.19562277289898944
1103	96-97	0.1905021876016697
1103	98-99	0.15892681717184232
1103	100	0.15148083659464007
1104	1	0.13709877499887568
1104	2	0.15582101829844675
1104	3	0.18890261869751157
1104	4	0.21245228748059475
1104	5	0.21043539236558928
1104	6	0.22936067119808
1104	7	0.22333242065794678
1104	8	0.19991316235993395
1104	9	0.21321707487356178
1104	10-11	0.255479318035583
1104	12-13	0.25834039312059076
1104	14-15	0.35247326116124356
1104	16-17	0.25108453985762225
1104	18-19	0.21650426782194643
1104	20-21	0.2945046147345636
1104	22-23	0.304240270475141
1104	24-25	0.32093609794046785
1104	26-27	0.28078692993280185
1104	28-29	0.22639442162601142
1104	30-31	0.22096646527632657
1104	32-33	0.2538774657680847
1104	34-35	0.2445639058753386
1104	36-37	0.2339228119142831
1104	38-39	0.21305611521196965
1104	40-41	0.22456266223549193
1104	42-43	0.25936310725513323
1104	44-45	0.2155916587668969
1104	46-47	0.16496552590891156
1104	48-49	0.15813616403477226
1104	50-51	0.15947455431961544
1104	52-53	0.1834715718798705
1104	54-55	0.1617030260944219
1104	56-57	0.17768651867967122
1104	58-59	0.20175267060652402
1104	60-61	0.22195802577109447
1104	62-63	0.20099177011442748
1104	64-65	0.19882057673925857
1104	66-67	0.18777255583459862
1104	68-69	0.19909268568520844
1104	70-71	0.21307224594593066
1104	72-73	0.1802916315855967
1104	74-75	0.20932839981498574
1104	76-77	0.14140063441764994
1104	78-79	0.09659156791877166
1104	80-81	0.1544458220755942
1104	82-83	0.17229891816629817
1104	84-85	0.1462747891492029
1104	86-87	0.13471348454037724
1104	88-89	0.11100801917800851
1104	90-91	0.10945223501197177
1104	92-93	0.0878337703110148
1104	94-95	0.1595833718858053
1104	96-97	0.1919340112798622
1104	98-99	0.1437009155756428
1104	100	0.15115772012769568
1105	1	0.15441358161768903
1105	2	0.20478781911128152
1105	3	0.19364041000752152
1105	4	0.1898976044948455
1105	5	0.1785235156732412
1105	6	0.20836226445621975
1105	7	0.23767946646662352
1105	8	0.21604960369931092
1105	9	0.3012252826909645
1105	10-11	0.29482464068092185
1105	12-13	0.27379575578287785
1105	14-15	0.33926826556721323
1105	16-17	0.29426889650998334
1105	18-19	0.3406770086187336
1105	20-21	0.32882373867390413
1105	22-23	0.31147948940424186
1105	24-25	0.2857353379445584
1105	26-27	0.32547185569989523
1105	28-29	0.31016573371425693
1105	30-31	0.31612934460464004
1105	32-33	0.3289792454955247
1105	34-35	0.3079182397390525
1105	36-37	0.28340485411023764
1105	38-39	0.3063534725412822
1105	40-41	0.3893334782671687
1105	42-43	0.29000460999089483
1105	44-45	0.27928818777390774
1105	46-47	0.2829211231097446
1105	48-49	0.23800375083806813
1105	50-51	0.2670000278732374
1105	52-53	0.19777670217395382
1105	54-55	0.17711180094406842
1105	56-57	0.18980773937516204
1105	58-59	0.1760258982513605
1105	60-61	0.13931203463750563
1105	62-63	0.1840709188702121
1105	64-65	0.23380129717950382
1105	66-67	0.2625633326263568
1105	68-69	0.22947741478053985
1105	70-71	0.2004081420655197
1105	72-73	0.24111973905645812
1105	74-75	0.2415145003023369
1105	76-77	0.20327231339708618
1105	78-79	0.16563838730978375
1105	80-81	0.19549044645071945
1105	82-83	0.15481376866236474
1105	84-85	0.11951180291583086
1105	86-87	0.10985830077348879
1105	88-89	0.09215640948032444
1105	90-91	0.1095521690239849
1105	92-93	0.09970220030866628
1105	94-95	0.1166388703790524
1105	96-97	0.15076366288889886
1105	98-99	0.10098792485965902
1105	100	0.049113217124183706
1106	1	0.22019252799762867
1106	2	0.21676085221233166
1106	3	0.23553218669584197
1106	4	0.2567205034234945
1106	5	0.1992546632723986
1106	6	0.20373185628739066
1106	7	0.23288587904598046
1106	8	0.19653417354923164
1106	9	0.27466567409960163
1106	10-11	0.2726110579598853
1106	12-13	0.2758701462732347
1106	14-15	0.3459590000339645
1106	16-17	0.32900709697708663
1106	18-19	0.30755098003942294
1106	20-21	0.2830801691472402
1106	22-23	0.29761091473874046
1106	24-25	0.34254419431799477
1106	26-27	0.31734164464613457
1106	28-29	0.3172439734658923
1106	30-31	0.33457390983284796
1106	32-33	0.2848434078992952
1106	34-35	0.27251615313218736
1106	36-37	0.2579194166078409
1106	38-39	0.2740952479097274
1106	40-41	0.34572159025969285
1106	42-43	0.2693989972097057
1106	44-45	0.2510112807018672
1106	46-47	0.22908280593148334
1106	48-49	0.19766432345409157
1106	50-51	0.21997121185754054
1106	52-53	0.18417306660185062
1106	54-55	0.19909823613865996
1106	56-57	0.1970841414345017
1106	58-59	0.18455898999793163
1106	60-61	0.17158888458728683
1106	62-63	0.22155120411799345
1106	64-65	0.2155806858242002
1106	66-67	0.2780149987571434
1106	68-69	0.29506597959677805
1106	70-71	0.2887823041101214
1106	72-73	0.23664497605973622
1106	74-75	0.27192932041266005
1106	76-77	0.2164826449095827
1106	78-79	0.19989678244262166
1106	80-81	0.2343725032674584
1106	82-83	0.24624263868455643
1106	84-85	0.2367018291963987
1106	86-87	0.24320045055237216
1106	88-89	0.23659695537870107
1106	90-91	0.257417527533228
1106	92-93	0.15712230812260586
1106	94-95	0.206871526741498
1106	96-97	0.29015497329514517
1106	98-99	0.30464665744656827
1106	100	0.2883562606500476
1107	1	0.22384609422761415
1107	2	0.283271963770634
1107	3	0.2847316883023723
1107	4	0.20591502444926135
1107	5	0.21980636740241977
1107	6	0.2660255059630643
1107	7	0.2727847917030317
1107	8	0.2794047488817739
1107	9	0.2855934204622699
1107	10-11	0.32760268152860306
1107	12-13	0.3018102267003826
1107	14-15	0.3723230167633531
1107	16-17	0.3643732283570955
1107	18-19	0.379271032830232
1107	20-21	0.41930995335561505
1107	22-23	0.4084708363239429
1107	24-25	0.36551995716856567
1107	26-27	0.4490547296547476
1107	28-29	0.3941524246897501
1107	30-31	0.3857072518863873
1107	32-33	0.30225527509722383
1107	34-35	0.3240395819853461
1107	36-37	0.3123352883939319
1107	38-39	0.3436969607599636
1107	40-41	0.4299526523140358
1107	42-43	0.45913063278548094
1107	44-45	0.37844106625180984
1107	46-47	0.3350628483921909
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2216	96-97	-0.7932640725450177
2216	98-99	-0.8562473761473086
2216	100	-0.9561287642856087
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77002.0
3	14687.0
4	8879.0
5	7363.0
6	6564.0
7	7242.0
8	8291.0
9	10088.0
10	11592.0
11	13323.0
12	15357.0
13	17453.0
14	20644.0
15	21906.0
16	21598.0
17	22129.0
18	20853.0
19	19179.0
20	18685.0
21	19495.0
22	21848.0
23	25907.0
24	32030.0
25	40200.0
26	48866.0
27	57135.0
28	70130.0
29	89248.0
30	108095.0
31	126648.0
32	163138.0
33	218372.0
34	301917.0
35	434955.0
36	725573.0
37	1398221.0
38	2200367.0
39	972223.0
40	2570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30044996490355	22.042207708776548	15.368215193314496	22.289127133005408
2	14.807243480127221	21.068681201346603	41.63838716845138	22.485688150074797
3	17.501836537051414	26.600598640117806	31.332545328843192	24.56501949398758
4	11.952835399778266	18.081254623129073	41.24548127778861	28.72042869930405
5	12.107171499645768	38.509836474573774	36.88455640650058	12.498435619279874
6	28.45747232878771	40.052797556927054	18.802057950542263	12.687672163742974
7	25.019636296987045	33.38639515163573	24.242226817579038	17.35174173379818
8	23.39837477047816	36.99886661554318	21.89264662434968	17.71011198962898
9	23.731583270115735	17.64270452667756	23.561277553717392	35.064434649489314
10-11	22.25211163933819	28.03842376702888	30.609822951269905	19.099641642363025
12-13	22.989054855207517	25.81168820198029	30.208783445243796	20.9904734975684
14-15	20.543212809866393	28.24882580662252	27.61010569581859	23.597855687692505
16-17	19.625973257158844	31.45806365286815	28.317312159636643	20.598650930336362
18-19	19.439721664423608	30.363342913744134	30.53880958372651	19.658125838105743
20-21	20.95642924432034	29.599879439420334	29.791951905519433	19.651739410739893
22-23	20.43759265919541	29.527448747106437	29.932172353908825	20.102786239789328
24-25	20.21033004848208	29.743059845024945	29.87127987554419	20.175330230948788
26-27	19.904758320166138	29.68650171200004	29.95734849098638	20.451391476847437
28-29	19.959207276085525	29.577353618326914	30.229696608339506	20.23374249724806
30-31	20.43494376437905	30.173790318357103	29.547922169238355	19.843343748025493
32-33	19.882532834398827	29.850043253439885	30.067050434369147	20.20037347779214
34-35	20.229722673534894	29.47099412353848	30.052274632757474	20.24700857016915
36-37	20.464876837973062	29.92249574892653	29.436025491776707	20.1766019213237
38-39	20.26821273219756	30.002386356931822	29.693849531646194	20.03555137922442
40-41	20.23064277576867	29.554525118326602	29.859784037981292	20.355048067923438
42-43	20.102279267486477	29.52787099226959	29.83679146540209	20.533058274841846
44-45	20.331156616492223	29.716229772249992	29.601803622667962	20.350809988589823
46-47	20.34068017089156	29.863661971221795	29.473583121485042	20.32207473640161
48-49	20.139153866314054	29.640981889533574	29.62205895332644	20.597805290825928
50-51	20.222258007991247	29.5313258868449	29.532096264499575	20.714319840664277
52-53	20.224616065374406	29.55757691498329	29.514956181128184	20.702850838514113
54-55	20.394698251825467	29.95232546164182	29.359164407552562	20.293811878980144
56-57	20.33476035469067	29.59402374404042	29.33128015271122	20.739935748557688
58-59	20.228619770805874	29.23130875933198	29.71455638446421	20.82551508539794
60-61	20.374024394364294	29.643220596682095	29.33258100483157	20.65017400412204
62-63	20.177194262214098	29.69381517024227	29.425642922048933	20.703347645494695
64-65	20.316804514771853	29.335480328131997	29.497343009453648	20.850372147642503
66-67	20.05110282974655	29.465322621246738	29.40458310342579	21.07899144558093
68-69	20.614414777516966	29.696274980236392	29.2671049329078	20.422205309338846
70-71	20.556386839890717	29.45709341618075	29.260002542628392	20.726517201300133
72-73	20.25565453521873	29.51517011083004	29.5157851198896	20.713390234061634
74-75	20.386706577032797	29.56250435069398	29.274800983206152	20.77598808906707
76-77	20.419781957305226	29.653061799576985	29.045511281811574	20.881644961306208
78-79	20.574479128971173	29.5729442445619	29.278077224289976	20.57449940217695
80-81	20.44464545160989	29.46168436825816	29.308081492786382	20.785588687345562
82-83	20.352418733719155	29.543142604574324	29.479369098441843	20.625069563264674
84-85	20.29360298411805	29.359771306638294	29.335706928446015	21.010918780797642
86-87	20.559364395872166	29.540551066773023	29.117269577926187	20.782814959428624
88-89	20.398998870400888	29.35958860020605	29.242513069120452	20.99889946027261
90-91	20.303527692210167	29.242300570929793	29.309529206273645	21.144642530586395
92-93	20.515270025593416	29.475789781257866	29.053161240133857	20.955778953014857
94-95	20.37344165313762	29.156593513719613	29.28357724594789	21.186387587194876
96-97	20.433970989443612	29.342252540094567	29.175245491171225	21.0485309792906
98-99	20.495299645250125	29.263762911176293	29.04955875213677	21.191378691436807
100	20.545516222633978	29.239569522774666	29.007450775283374	21.207463479307982
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1635.0
1	1098.5
2	650.5
3	760.5
4	751.0
5	662.0
6	623.5
7	694.0
8	751.0
9	759.0
10	731.5
11	693.5
12	749.5
13	880.0
14	1013.0
15	1268.0
16	1656.5
17	2253.5
18	3237.0
19	4760.5
20	7126.5
21	10639.0
22	16199.0
23	24750.5
24	35113.0
25	48303.0
26	66691.5
27	90420.5
28	120158.5
29	150499.5
30	177510.0
31	203635.0
32	229871.0
33	253638.0
34	274508.0
35	290819.0
36	299798.0
37	306234.5
38	310281.5
39	307053.0
40	303438.5
41	296967.0
42	285153.0
43	275518.0
44	262179.5
45	248901.0
46	239281.0
47	229697.0
48	221165.0
49	212013.5
50	201511.5
51	184686.5
52	164480.5
53	148042.0
54	136636.5
55	128587.0
56	114889.0
57	100302.5
58	87797.0
59	74600.0
60	60412.5
61	45891.0
62	34766.0
63	26058.5
64	19357.0
65	14207.0
66	10282.5
67	7153.0
68	4811.5
69	3360.5
70	2387.0
71	1716.5
72	1284.5
73	959.0
74	721.5
75	525.5
76	382.0
77	316.5
78	252.0
79	192.5
80	153.5
81	116.0
82	86.0
83	72.0
84	68.0
85	41.0
86	30.5
87	33.0
88	26.0
89	21.0
90	13.5
91	11.5
92	13.0
93	12.5
94	14.5
95	12.0
96	7.5
97	11.5
98	17.5
99	18.5
100	30.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07923216022978002
2	0.013635553414949349
3	0.01808163574747496
4	0.011270616004031475
5	0.009175957154361357
6	0.007662397211373917
7	0.020054669244583583
8	0.009392180003359563
9	0.009811111773293586
10-11	0.013770692695573229
12-13	0.015541017271746038
14-15	0.010236800507258803
16-17	0.013115267184547419
18-19	0.008743511456364945
20-21	0.013554469846575025
22-23	0.013784206623635616
24-25	0.015716698336557082
26-27	0.005223133196112908
28-29	0.012223347932429818
30-31	0.010412481572069846
32-33	0.012696335414613395
34-35	0.009155686262267775
36-37	0.01026382836338358
38-39	0.019818175503491797
40-41	0.013135538076640998
42-43	0.008371878434649278
44-45	0.020966859388794765
46-47	0.018878957503155838
48-49	0.01816271931584928
50-51	0.010871955126191034
52-53	0.010703031025411185
54-55	0.011554408493341621
56-57	0.020210079417301047
58-59	0.002033846173389373
60-61	0.00706778437662885
62-63	0.0158112958329938
64-65	0.024804814958512916
66-67	0.01304769754423548
68-69	0.014513958739004561
70-71	0.025899443131566332
72-73	0.02037900351808089
74-75	0.02064928207932865
76-77	0.00969624338476329
78-79	0.011412512248686548
80-81	0.019919529963959706
82-83	0.012601737918176679
84-85	0.011757117414277438
86-87	0.017142417747139
88-89	0.015277495674529474
90-91	0.015405877991122158
92-93	0.008162412549682268
94-95	0.01604778957408558
96-97	0.01329094824935846
98-99	0.01023004354322761
100	0.007338062937876608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7399773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.168937851564344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34429996834021	47.59419402169705
2	15.502020153570871	19.584922953092327
3	4.827299330895577	9.148061142727224
4	1.9303317238124909	4.877480187776573
5	0.87469137086355	2.7626662422689607
6	0.4720681452209069	1.7892025996297634
7	0.26359751001678794	1.1655822309654311
8	0.16779836529675723	0.8479715607219959
9	0.1121340971432155	0.6375052632132953
>10	0.43845506038919685	4.843167396408119
>50	0.03376991834401576	1.4760122736945576
>100	0.03066470214281756	3.823868857947897
>500	0.0021844582532548774	0.9114917906486143
>1k	6.851957104595754E-4	0.5378734792082971
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.054178418716358E-5	0.0
4	0.0	0.0	0.0	5.405571224955144E-5	0.0
5	0.0	0.0	0.0	6.75696403119393E-5	0.0
6	0.0	0.0	0.0	6.75696403119393E-5	0.0
7	0.0	0.0	0.0	6.75696403119393E-5	0.0
8	0.0	0.0	0.0	8.108356837432716E-5	0.0
9	4.054178418716358E-5	0.0	0.0	1.0811142449910288E-4	0.0
10-11	4.054178418716358E-5	0.0	0.0	1.4189624465507254E-4	0.0
12-13	4.054178418716358E-5	6.75696403119393E-6	0.0	2.027089209358179E-4	0.0
14-15	5.405571224955144E-5	1.351392806238786E-5	0.0	2.770355252789511E-4	0.0
16-17	5.405571224955144E-5	1.351392806238786E-5	0.0	3.378482015596965E-4	0.0
18-19	6.75696403119393E-5	1.351392806238786E-5	0.0	3.648760576844722E-4	0.0
20-21	8.108356837432716E-5	1.351392806238786E-5	0.0	3.85146949778054E-4	0.0
22-23	8.108356837432716E-5	2.027089209358179E-5	0.0	5.473140865267082E-4	0.0
24-25	8.108356837432716E-5	2.702785612477572E-5	0.0	8.243496118056595E-4	0.0
26-27	8.784053240552109E-5	2.702785612477572E-5	0.0	0.0014122054825195314	0.0
28-29	9.459749643671502E-5	2.702785612477572E-5	0.0	0.0033176693393162193	0.0
30-31	9.459749643671502E-5	2.702785612477572E-5	0.0	0.009885438377636719	0.0
32-33	9.459749643671502E-5	2.702785612477572E-5	0.0	0.02527780244069649	0.0
34-35	9.459749643671502E-5	2.702785612477572E-5	0.0	0.0515353646659161	0.0
36-37	9.459749643671502E-5	4.054178418716358E-5	0.0	0.09123252834918044	0.0
38-39	1.0811142449910288E-4	4.729874821835751E-5	0.0	0.1443625365264583	0.0
40-41	1.0811142449910288E-4	5.405571224955144E-5	0.0	0.19952639087712554	0.0
42-43	1.0811142449910288E-4	5.405571224955144E-5	0.0	0.25775790689795486	0.0
44-45	1.0811142449910288E-4	5.405571224955144E-5	0.0	0.3342399827670389	0.0
46-47	1.0811142449910288E-4	6.0812676280745366E-5	0.0	0.42735770408092244	0.0
48-49	1.0811142449910288E-4	6.75696403119393E-5	0.0	0.5265904778430366	0.0
50-51	1.0811142449910288E-4	6.75696403119393E-5	0.0	0.6263908365837709	0.0
52-53	1.1486838853029681E-4	6.75696403119393E-5	0.0	0.7262452510367547	0.0
54-55	1.351392806238786E-4	6.75696403119393E-5	0.0	0.8414920295527984	0.0
56-57	1.4865320868626646E-4	6.75696403119393E-5	0.0	0.981820658552634	0.0
58-59	1.6216713674865432E-4	6.75696403119393E-5	0.0	1.1379740973135257	0.0
60-61	1.8919499287343004E-4	6.75696403119393E-5	0.0	1.2965262583054913	0.0
62-63	1.8919499287343004E-4	6.75696403119393E-5	0.0	1.4607745399757532	0.0
64-65	1.8919499287343004E-4	6.75696403119393E-5	0.0	1.6403881578529504	0.0
66-67	1.8919499287343004E-4	6.75696403119393E-5	0.0	1.8501310783452412	0.0
68-69	1.8919499287343004E-4	6.75696403119393E-5	0.0	2.0887397491787922	0.0
70-71	1.95951956904624E-4	6.75696403119393E-5	0.0	2.345024637917947	0.0
72-73	2.0946588496701182E-4	6.75696403119393E-5	0.0	2.605755608989627	0.0
74-75	2.1622284899820577E-4	6.75696403119393E-5	0.0	2.8730138073154405	0.0
76-77	2.1622284899820577E-4	6.75696403119393E-5	0.0	3.1649484382831745	0.0
78-79	2.1622284899820577E-4	6.75696403119393E-5	0.0	3.498134334661347	0.0
80-81	2.1622284899820577E-4	6.75696403119393E-5	0.0	3.8603278776254353	0.0
82-83	2.1622284899820577E-4	7.432660434313323E-5	0.0	4.229150002304125	0.0
84-85	2.1622284899820577E-4	8.108356837432716E-5	0.0	4.6048507163665695	0.0
86-87	2.1622284899820577E-4	8.108356837432716E-5	0.0	5.004721090768595	0.0
88	2.1622284899820577E-4	8.108356837432716E-5	0.0	5.3262579811569895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7495	0.0	44.73917	1
GTATCAA	13405	0.0	35.504528	1
TCAACGC	13935	0.0	32.95199	4
ATCAACG	13895	0.0	32.94849	3
CAACGCA	15580	0.0	29.472584	5
TATCAAC	16615	0.0	28.00722	2
AACGCAG	16825	0.0	27.375689	6
CGCAGAG	17565	0.0	25.684963	8
ACGCAGA	18200	0.0	24.998089	7
GTACATG	19890	0.0	23.077305	1
TACATGG	19495	0.0	22.27839	2
GTGGTAT	4470	0.0	21.3579	1
ACATGGG	19695	0.0	21.288446	3
GCAGAGT	20475	0.0	21.02572	9
TGGTATC	3910	0.0	20.676968	2
AGAGTAC	18840	0.0	17.749157	10-11
ATACCGC	455	0.0	17.560284	6
CAGAGTA	20690	0.0	17.422827	10-11
TCTGTCG	1750	0.0	16.918364	8
GAGTACT	9775	0.0	15.962059	12-13
>>END_MODULE