FastQCFastQC Report
Wed 25 May 2016
SRR2049466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7399773
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171560.2318449498383261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168690.2279664524844208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84730.11450351247261233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135950.058.5051771
GTATCAA252400.040.3323861
ATCAACG300350.033.4282883
TCAACGC305000.032.9494634
TATCAAC319750.031.561822
CAACGCA321800.031.2877245
GTGGTAT54300.030.9424821
TGGTATC53100.030.0971182
AACGCAG336950.029.9470656
CGCAGAG364400.027.3945318
ACGCAGA372650.026.9141717
GCAGAGT401100.024.536459
GTACATG199000.021.8054281
GAGTACT300450.021.70443512-13
CAGAGTA408150.021.24536910-11
TACATGG195950.020.8936062
GGACCGT3650.020.6022596
AGAGTAC387250.020.41979210-11
ACATGGG196200.020.2920063
GTACTTT329450.019.7154314-15
Produced by FastQC (version 0.11.5)