##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7399773 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00279089642344 33.0 31.0 34.0 30.0 34.0 2 32.17758869089633 33.0 31.0 34.0 30.0 34.0 3 32.133732075294745 34.0 31.0 34.0 30.0 34.0 4 35.850336219773226 37.0 35.0 37.0 35.0 37.0 5 35.90417179013464 37.0 35.0 37.0 35.0 37.0 6 35.96563705400152 37.0 35.0 37.0 35.0 37.0 7 35.954061293501844 37.0 35.0 37.0 35.0 37.0 8 35.96533136894875 37.0 35.0 37.0 35.0 37.0 9 37.69059618450458 39.0 37.0 39.0 35.0 39.0 10-11 37.66360096451608 39.0 37.0 39.0 35.0 39.0 12-13 37.64680220595956 39.0 37.0 39.0 35.0 39.0 14-15 38.95677205233187 40.0 38.0 41.0 36.0 41.0 16-17 38.97047909442628 40.0 38.0 41.0 36.0 41.0 18-19 39.05993798728691 40.0 38.5 41.0 36.0 41.0 20-21 39.09426397269213 40.0 39.0 41.0 36.0 41.0 22-23 39.06675035842316 40.0 39.0 41.0 36.0 41.0 24-25 39.052164370447585 40.0 39.0 41.0 35.0 41.0 26-27 38.96161280893347 40.0 39.0 41.0 35.0 41.0 28-29 38.888595906928494 40.0 38.0 41.0 35.0 41.0 30-31 38.777660206603635 40.0 38.0 41.0 35.0 41.0 32-33 38.63867175384975 40.0 38.0 41.0 35.0 41.0 34-35 38.52655628760504 40.0 38.0 41.0 34.5 41.0 36-37 38.39076766273776 40.0 38.0 41.0 34.0 41.0 38-39 38.2799387224446 40.0 38.0 41.0 34.0 41.0 40-41 38.18796488757155 40.0 38.0 41.0 34.0 41.0 42-43 38.047308207968 40.0 38.0 41.0 33.5 41.0 44-45 37.875528816897486 40.0 38.0 41.0 33.0 41.0 46-47 37.626967070476354 40.0 38.0 41.0 33.0 41.0 48-49 37.38381886849773 40.0 38.0 41.0 33.0 41.0 50-51 37.23816108953612 40.0 37.0 41.0 32.0 41.0 52-53 37.133220437978295 40.0 37.0 41.0 32.0 41.0 54-55 37.00741610587244 40.0 37.0 41.0 31.5 41.0 56-57 36.88173008820676 40.0 37.0 41.0 31.0 41.0 58-59 36.72685391835668 40.0 36.0 41.0 31.0 41.0 60-61 36.560530843311 39.5 36.0 41.0 31.0 41.0 62-63 36.36481504229927 39.0 35.5 41.0 31.0 41.0 64-65 36.16446585591207 39.0 35.0 41.0 30.5 41.0 66-67 35.92235423978546 39.0 35.0 41.0 30.0 41.0 68-69 35.64443260624346 38.0 35.0 40.0 30.0 41.0 70-71 35.365441818282804 37.5 35.0 40.0 29.5 41.0 72-73 35.035447438725484 37.0 35.0 39.5 29.0 41.0 74-75 34.706355992271654 36.5 34.5 39.0 29.0 41.0 76-77 34.37207945432921 36.0 34.0 39.0 29.0 40.5 78-79 34.04199838292337 36.0 34.0 38.5 29.0 40.0 80-81 33.70384422603234 35.0 34.0 37.0 29.0 39.0 82-83 33.40024937251454 35.0 34.0 37.0 29.0 39.0 84-85 33.08399088728803 35.0 34.0 36.5 29.0 38.5 86-87 32.808432096498095 35.0 34.0 36.0 28.0 37.0 88-89 32.56263516461924 35.0 34.0 36.0 27.5 37.0 90-91 32.34562904835054 35.0 34.0 35.5 27.0 36.5 92-93 32.16677362940728 35.0 34.0 35.0 27.0 36.0 94-95 31.995512362338683 35.0 33.0 35.0 27.0 36.0 96-97 31.844250830397094 35.0 33.0 35.0 26.0 36.0 98-99 31.704536071579493 35.0 33.0 35.0 26.0 36.0 100 31.59076014899376 35.0 33.0 35.0 26.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3329159818367131 1101 2 0.35379182241344154 1101 3 0.44816543787551666 1101 4 0.24956971133130423 1101 5 0.20179932248856858 1101 6 0.21266125809701464 1101 7 0.23010156079241284 1101 8 0.2560374027155561 1101 9 0.2965053174935477 1101 10-11 0.3186883803167291 1101 12-13 0.2988496630312696 1101 14-15 0.4005297691598031 1101 16-17 0.3783420031162663 1101 18-19 0.37131745067839717 1101 20-21 0.3894104238560345 1101 22-23 0.40605247123110644 1101 24-25 0.3781788621757016 1101 26-27 0.37792417259500155 1101 28-29 0.3945608130427729 1101 30-31 0.3679584576414072 1101 32-33 0.34633510531123335 1101 34-35 0.3508561793492717 1101 36-37 0.35374209671538637 1101 38-39 0.34146285382054486 1101 40-41 0.3556280010761981 1101 42-43 0.35962560118342424 1101 44-45 0.3625220851336479 1101 46-47 0.3080427741342504 1101 48-49 0.2684403084269036 1101 50-51 0.2677197291516791 1101 52-53 0.3306977142015626 1101 54-55 0.3213801519598789 1101 56-57 0.3240066429034414 1101 58-59 0.3189459793868039 1101 60-61 0.24397076235920423 1101 62-63 0.2819082716652872 1101 64-65 0.2822249191292556 1101 66-67 0.30206273000287354 1101 68-69 0.2813801497564512 1101 70-71 0.3012972173843451 1101 72-73 0.32351488983870524 1101 74-75 0.33090926621733985 1101 76-77 0.3363761325247623 1101 78-79 0.32758597097929254 1101 80-81 0.2949022086016839 1101 82-83 0.26974938510017665 1101 84-85 0.25191691952255013 1101 86-87 0.24490800036130622 1101 88-89 0.2765829172559151 1101 90-91 0.3173444080458978 1101 92-93 0.28294341105322474 1101 94-95 0.2429379832486518 1101 96-97 0.22747434922849408 1101 98-99 0.32354017382244393 1101 100 0.32948671356351156 1102 1 0.3079725411253804 1102 2 0.2876229504163703 1102 3 0.35744913134943346 1102 4 0.26094501756931265 1102 5 0.23388394966610093 1102 6 0.2244162656061306 1102 7 0.21491700013684323 1102 8 0.21354404319018272 1102 9 0.2535864675055066 1102 10-11 0.25410785671550684 1102 12-13 0.26535333267814565 1102 14-15 0.3559705305001799 1102 16-17 0.38383954513788154 1102 18-19 0.3489464092425649 1102 20-21 0.3445024788843227 1102 22-23 0.3173371888409875 1102 24-25 0.32928658033723224 1102 26-27 0.34023956375398967 1102 28-29 0.3582184682506764 1102 30-31 0.3588497424319499 1102 32-33 0.4144179811433375 1102 34-35 0.441400528968245 1102 36-37 0.394485048487752 1102 38-39 0.3564347734295197 1102 40-41 0.4369219702906548 1102 42-43 0.40769623009979483 1102 44-45 0.48505794682215964 1102 46-47 0.45419257511535704 1102 48-49 0.4657075948885634 1102 50-51 0.4180501770887446 1102 52-53 0.44845417925512976 1102 54-55 0.4482428578354316 1102 56-57 0.4410156085209209 1102 58-59 0.5023534662061309 1102 60-61 0.4644329310582549 1102 62-63 0.42049855841814576 1102 64-65 0.40111069463093685 1102 66-67 0.4255510745959725 1102 68-69 0.4578170265689394 1102 70-71 0.4448945704669427 1102 72-73 0.43455907093886736 1102 74-75 0.39731226736285663 1102 76-77 0.38846373417651137 1102 78-79 0.3894410218813533 1102 80-81 0.39395470051825043 1102 82-83 0.3685545684991709 1102 84-85 0.3352725223432458 1102 86-87 0.3531633904888736 1102 88-89 0.3423743539998938 1102 90-91 0.3412650151095207 1102 92-93 0.36156519806107923 1102 94-95 0.34520335010654435 1102 96-97 0.36756190087155716 1102 98-99 0.43396198135468467 1102 100 0.4267422792423474 1103 1 0.28583846449740236 1103 2 0.2750819086480547 1103 3 0.34922647897433023 1103 4 0.18764625136932267 1103 5 0.19309654945173804 1103 6 0.18958916382969448 1103 7 0.20000704924695611 1103 8 0.16889095672409127 1103 9 0.24751106444570325 1103 10-11 0.25056570858443195 1103 12-13 0.24081266386786382 1103 14-15 0.33824908070623394 1103 16-17 0.3509992194370781 1103 18-19 0.30311068534351904 1103 20-21 0.305969464054769 1103 22-23 0.30831683224305806 1103 24-25 0.31481355932348976 1103 26-27 0.2831614784943497 1103 28-29 0.32258780871668336 1103 30-31 0.3240687539978211 1103 32-33 0.3062687139224778 1103 34-35 0.3529719840944239 1103 36-37 0.3404873821565104 1103 38-39 0.3656460773944943 1103 40-41 0.3883275037725724 1103 42-43 0.42842568180977025 1103 44-45 0.3980659308648029 1103 46-47 0.3624606854751917 1103 48-49 0.3827113536621596 1103 50-51 0.3636812953730413 1103 52-53 0.35684883320547556 1103 54-55 0.364226969584621 1103 56-57 0.3572198757328451 1103 58-59 0.31443864720100123 1103 60-61 0.2779532408874843 1103 62-63 0.3238919850624882 1103 64-65 0.2865730911067814 1103 66-67 0.3458838264484072 1103 68-69 0.3456420466913883 1103 70-71 0.26874788833383434 1103 72-73 0.34071472551299564 1103 74-75 0.3182502121495716 1103 76-77 0.30659262275559485 1103 78-79 0.3042113965016142 1103 80-81 0.28108286701244367 1103 82-83 0.27484527141783843 1103 84-85 0.2785632395606257 1103 86-87 0.23077819275259515 1103 88-89 0.23724981520778954 1103 90-91 0.16422397840532454 1103 92-93 0.24282859937881796 1103 94-95 0.25078695198754986 1103 96-97 0.24316220841300407 1103 98-99 0.2718265910326494 1103 100 0.2687421207649727 1104 1 0.16515288642344217 1104 2 0.17190119931429848 1104 3 0.2610665739895026 1104 4 0.15662514908073888 1104 5 0.16900526636072044 1104 6 0.13969373658184736 1104 7 0.11795621655118538 1104 8 0.11835096369334508 1104 9 0.18239410598616956 1104 10-11 0.19931042300937918 1104 12-13 0.17039690617524883 1104 14-15 0.21070384035071754 1104 16-17 0.22400776990600235 1104 18-19 0.20357076532834384 1104 20-21 0.20412955383905285 1104 22-23 0.18353730118757028 1104 24-25 0.18790814702800418 1104 26-27 0.1761520438891111 1104 28-29 0.1638746767247099 1104 30-31 0.19636829435016523 1104 32-33 0.21264431655988147 1104 34-35 0.23844550696756528 1104 36-37 0.19170200714342656 1104 38-39 0.193229571800984 1104 40-41 0.22938846811223357 1104 42-43 0.2226733901930018 1104 44-45 0.21455812876771319 1104 46-47 0.22126149265506712 1104 48-49 0.2146935317615828 1104 50-51 0.18276529141301978 1104 52-53 0.21277462437788586 1104 54-55 0.2040395674967712 1104 56-57 0.21101036202363588 1104 58-59 0.15169546497257613 1104 60-61 0.1241503065359737 1104 62-63 0.14779534256140892 1104 64-65 0.11991209934672753 1104 66-67 0.14338731117072 1104 68-69 0.15301343578834548 1104 70-71 0.09625270169072309 1104 72-73 0.11119586378114832 1104 74-75 0.14229089767903957 1104 76-77 0.17938456604216668 1104 78-79 0.16099040635106832 1104 80-81 0.12948906784208702 1104 82-83 0.1786898751251229 1104 84-85 0.14922881182273073 1104 86-87 0.14496986545962187 1104 88-89 0.1512936954506614 1104 90-91 0.1299997045136294 1104 92-93 0.13779166812266652 1104 94-95 0.08897772752244748 1104 96-97 0.11259901975483899 1104 98-99 0.14835129009117765 1104 100 0.1297888346370648 1105 1 0.19327127162425484 1105 2 0.20520834044470604 1105 3 0.2951540925206615 1105 4 0.19344627284924343 1105 5 0.17454739580529122 1105 6 0.14366546024864135 1105 7 0.14617828441586056 1105 8 0.12704769180243147 1105 9 0.14737506549307966 1105 10-11 0.12899631599172778 1105 12-13 0.1669043746566743 1105 14-15 0.22930429037457145 1105 16-17 0.23699902926639282 1105 18-19 0.2317741845511918 1105 20-21 0.22122952485999292 1105 22-23 0.22256880039716975 1105 24-25 0.22584214730837715 1105 26-27 0.18612019125193058 1105 28-29 0.2509222744836208 1105 30-31 0.23928602363422158 1105 32-33 0.22053913504804967 1105 34-35 0.19329692433057488 1105 36-37 0.1586727753815893 1105 38-39 0.19078476594605576 1105 40-41 0.21567916169924217 1105 42-43 0.15819168983623655 1105 44-45 0.16944088702285853 1105 46-47 0.20096398468582777 1105 48-49 0.17275481787591218 1105 50-51 0.13070161756967025 1105 52-53 0.15840965226375658 1105 54-55 0.18570197366141628 1105 56-57 0.17978866079693745 1105 58-59 0.13869165303519537 1105 60-61 0.12613630804269604 1105 62-63 0.09817975013775992 1105 64-65 0.0662086876887713 1105 66-67 0.07788241358861825 1105 68-69 0.14443435317014064 1105 70-71 0.17241498043274817 1105 72-73 0.2000340825915572 1105 74-75 0.15442221990992522 1105 76-77 0.16514658707878738 1105 78-79 0.14277318388924698 1105 80-81 0.134595119140279 1105 82-83 0.08482266864778154 1105 84-85 0.07782704158136511 1105 86-87 0.06042503348037087 1105 88-89 0.05386534631251294 1105 90-91 0.08387885585381127 1105 92-93 0.09911024433531423 1105 94-95 0.016536936419576875 1105 96-97 0.06417035323383757 1105 98-99 0.11487262561075795 1105 100 0.08294263536138402 1106 1 0.1597282851773727 1106 2 0.1326935200314594 1106 3 0.22528038896874136 1106 4 0.1114668481136647 1106 5 0.15403024030335644 1106 6 0.13720424907501183 1106 7 0.10474103646242128 1106 8 0.1071060430549835 1106 9 0.1589315301964973 1106 10-11 0.12988971676971772 1106 12-13 0.12480395026425839 1106 14-15 0.20085045143423486 1106 16-17 0.25366721775080947 1106 18-19 0.21868702074488766 1106 20-21 0.18975768332059317 1106 22-23 0.212556146951421 1106 24-25 0.21876754693700207 1106 26-27 0.2525540140335849 1106 28-29 0.228649951040083 1106 30-31 0.26243234792507053 1106 32-33 0.27467170542418984 1106 34-35 0.2391433269048875 1106 36-37 0.24698014765816367 1106 38-39 0.18241588338292303 1106 40-41 0.21605518194822082 1106 42-43 0.20529807528252775 1106 44-45 0.22810305776388873 1106 46-47 0.1688103803362253 1106 48-49 0.1697031520913228 1106 50-51 0.17850513257918976 1106 52-53 0.18349999414479612 1106 54-55 0.23118167730075356 1106 56-57 0.2342633026252372 1106 58-59 0.1530885090607086 1106 60-61 0.14292461771284337 1106 62-63 0.14037177786109112 1106 64-65 0.1361442953246339 1106 66-67 0.1482195733920335 1106 68-69 0.14049247160621547 1106 70-71 0.17447784261274535 1106 72-73 0.20403537563177565 1106 74-75 0.19832007838780186 1106 76-77 0.1679363559630005 1106 78-79 0.181097956817446 1106 80-81 0.12350135338567725 1106 82-83 0.21456346341746269 1106 84-85 0.21179233894383032 1106 86-87 0.18195693561263226 1106 88-89 0.18064723405601768 1106 90-91 0.18824048648657055 1106 92-93 0.20814475699806678 1106 94-95 0.16228133681545387 1106 96-97 0.17627230088648105 1106 98-99 0.14521880813454757 1106 100 0.14276031149808333 1107 1 0.09204391984087579 1107 2 0.08682485308306553 1107 3 0.18619946543155663 1107 4 0.09064444858052667 1107 5 0.10145189258167875 1107 6 0.09840109772572703 1107 7 0.07995110964509422 1107 8 0.09046032165894502 1107 9 0.09312980761988143 1107 10-11 0.10741903847163314 1107 12-13 0.10625292195040714 1107 14-15 0.1378316660922394 1107 16-17 0.1374569366246945 1107 18-19 0.16839112058457317 1107 20-21 0.1816964624242985 1107 22-23 0.14424381758243499 1107 24-25 0.17556950261151627 1107 26-27 0.1435332683215833 1107 28-29 0.18041732773297525 1107 30-31 0.1666256279980587 1107 32-33 0.13645675730942486 1107 34-35 0.17009854192510687 1107 36-37 0.18599875524206055 1107 38-39 0.1861702770265623 1107 40-41 0.21679457180191974 1107 42-43 0.13254222100392354 1107 44-45 0.21717288818936709 1107 46-47 0.21637173203566107 1107 48-49 0.20988135458799206 1107 50-51 0.19222540840610236 1107 52-53 0.20959407848933864 1107 54-55 0.26963863636272833 1107 56-57 0.22467300150972136 1107 58-59 0.23115289103267855 1107 60-61 0.2182781886383296 1107 62-63 0.27136845579696 1107 64-65 0.22921613978294175 1107 66-67 0.2340897579921446 1107 68-69 0.24176207530626925 1107 70-71 0.21421518824828922 1107 72-73 0.2435121127979727 1107 74-75 0.2821284283230696 1107 76-77 0.27146283353528844 1107 78-79 0.2575864712963494 1107 80-81 0.286746478453594 1107 82-83 0.2666549803313174 1107 84-85 0.24696771575746368 1107 86-87 0.1948448423600624 1107 88-89 0.21267674525478242 1107 90-91 0.20577213078255596 1107 92-93 0.22454696656480877 1107 94-95 0.19342432140751242 1107 96-97 0.2313186018410036 1107 98-99 0.24110728001858206 1107 100 0.2604364764844327 1108 1 0.08413626882926195 1108 2 0.12982649490985665 1108 3 0.16976302033353363 1108 4 0.12291589171041295 1108 5 0.1146547566298679 1108 6 0.11441012725326516 1108 7 0.09577723838152252 1108 8 0.06585445138093604 1108 9 0.11712900022160255 1108 10-11 0.06686045300665455 1108 12-13 0.08412886653037077 1108 14-15 0.1419057184713779 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100 -0.26686668469472963 2215 1 -0.3695703425375214 2215 2 -0.3824544682435622 2215 3 -0.26179236278834495 2215 4 -0.2128548210758865 2215 5 -0.22066514965549544 2215 6 -0.19810870513342138 2215 7 -0.24068764384777808 2215 8 -0.24093591266443326 2215 9 -0.36158682166440315 2215 10-11 -0.39682599912868 2215 12-13 -0.4151377843706783 2215 14-15 -0.5641616470947284 2215 16-17 -0.57488476278197 2215 18-19 -0.486047840514928 2215 20-21 -0.5070705161984748 2215 22-23 -0.5094429162557361 2215 24-25 -0.553701864345534 2215 26-27 -0.4571647563710215 2215 28-29 -0.4596406451141988 2215 30-31 -0.4963085319548668 2215 32-33 -0.4830196103760116 2215 34-35 -0.47587650975213336 2215 36-37 -0.4865656954434101 2215 38-39 -0.42955904439739356 2215 40-41 -0.5081571536550769 2215 42-43 -0.39776098135748583 2215 44-45 -0.40860336210899106 2215 46-47 -0.4030706627223424 2215 48-49 -0.3801976766074091 2215 50-51 -0.34092015048734936 2215 52-53 -0.41365945888596656 2215 54-55 -0.36509946738066645 2215 56-57 -0.2813856232986254 2215 58-59 -0.27649741206585077 2215 60-61 -0.2883427198883268 2215 62-63 -0.32139499769861146 2215 64-65 -0.35549815316974076 2215 66-67 -0.345935787675522 2215 68-69 -0.34527460115238995 2215 70-71 -0.326054454998399 2215 72-73 -0.2803617231865161 2215 74-75 -0.26692251706253245 2215 76-77 -0.3202156763399202 2215 78-79 -0.27431780835159714 2215 80-81 -0.3008141253014287 2215 82-83 -0.33510580351131836 2215 84-85 -0.31692112536473616 2215 86-87 -0.27193430876395297 2215 88-89 -0.2533830227094498 2215 90-91 -0.25267096227931773 2215 92-93 -0.2545901816430316 2215 94-95 -0.2726928003747062 2215 96-97 -0.24089979679335016 2215 98-99 -0.3225660499923535 2215 100 -0.32792367396656275 2216 1 -0.29788517213750865 2216 2 -0.30330868446561965 2216 3 -0.22111904995842124 2216 4 -0.1919952993998706 2216 5 -0.23190600387533067 2216 6 -0.2313985405427701 2216 7 -0.2784180948234223 2216 8 -0.23521327762258437 2216 9 -0.3814620114044871 2216 10-11 -0.3898150347911695 2216 12-13 -0.40679311102122284 2216 14-15 -0.5841826637882832 2216 16-17 -0.5582048489454365 2216 18-19 -0.5252786132143754 2216 20-21 -0.5035090901449308 2216 22-23 -0.5198190701793521 2216 24-25 -0.5468626832669159 2216 26-27 -0.5429687255251139 2216 28-29 -0.5853893154935577 2216 30-31 -0.5421272879705441 2216 32-33 -0.5365280851947105 2216 34-35 -0.5497066286065646 2216 36-37 -0.6269125356318312 2216 38-39 -0.4848990905613846 2216 40-41 -0.5494633871183936 2216 42-43 -0.6318930917074681 2216 44-45 -0.6377738771418464 2216 46-47 -0.6258786297582759 2216 48-49 -0.6292997873479251 2216 50-51 -0.5852580150947659 2216 52-53 -0.6346121927512272 2216 54-55 -0.671114992268123 2216 56-57 -0.5980207533463258 2216 58-59 -0.5832635984048977 2216 60-61 -0.6091848507865976 2216 62-63 -0.6003099328839809 2216 64-65 -0.6754863736703953 2216 66-67 -0.6252824276864928 2216 68-69 -0.6577644768256121 2216 70-71 -0.6299777088689495 2216 72-73 -0.6275536707980507 2216 74-75 -0.5941275491589337 2216 76-77 -0.5303814434402838 2216 78-79 -0.5378214673647506 2216 80-81 -0.5895415344923265 2216 82-83 -0.6034490467411118 2216 84-85 -0.6318772032540636 2216 86-87 -0.5821535521208148 2216 88-89 -0.5783816573079292 2216 90-91 -0.5343207556744005 2216 92-93 -0.5536109575808332 2216 94-95 -0.5821070848970642 2216 96-97 -0.5825968896196869 2216 98-99 -0.616642325020436 2216 100 -0.599297473865203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 652.0 3 52.0 4 64.0 5 122.0 6 368.0 7 965.0 8 2378.0 9 4045.0 10 5803.0 11 8110.0 12 12255.0 13 17768.0 14 22262.0 15 28885.0 16 35999.0 17 51117.0 18 51355.0 19 25815.0 20 17554.0 21 16032.0 22 16504.0 23 17955.0 24 21170.0 25 26030.0 26 32661.0 27 41986.0 28 55542.0 29 72788.0 30 95796.0 31 124711.0 32 162055.0 33 212983.0 34 290475.0 35 416001.0 36 683559.0 37 1370984.0 38 2313366.0 39 1138966.0 40 4640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.99576973558411 22.05141029789262 15.579674107249788 22.373145859273475 2 14.871726470528218 21.345100721332937 41.001636131270516 22.78153667686833 3 17.073794020438193 26.684264503789507 31.804272914858334 24.437668560913963 4 11.866093730172533 18.56608304065544 40.58681259546745 28.981010633704575 5 11.62106005254187 38.42290054524658 36.83475045721049 13.12128894500107 6 28.16599038292354 40.411843420740745 18.218159684767897 13.204006511567817 7 24.499886685713196 34.01163792456877 23.917193135519156 17.571282254198877 8 23.4493004042151 36.759262750357344 21.388750708974452 18.402686136453106 9 23.79666241113072 17.111105975818447 23.37310887779936 35.719122735251474 10-11 22.222857376841155 28.05465789288401 30.114626489217983 19.607858241056856 12-13 23.353952884770926 25.828596363699262 30.134363581153096 20.683087170376712 14-15 20.639693947368386 27.933140651747017 28.218575083316743 23.20859031756785 16-17 19.061787435911885 31.208058679637873 28.949090735621212 20.781063148829023 18-19 18.774805659579016 29.346042101561764 32.356479043343626 19.52267319551559 20-21 20.238343797843527 28.536990256322724 31.96532515254184 19.2593407932919 22-23 19.69980025062931 28.452899838954522 32.182399379007975 19.664900531408193 24-25 19.57416126143329 28.626803822225355 32.09600483690514 19.70303007943622 26-27 19.286334864596522 28.617425156150063 32.15474988219233 19.941490097061084 28-29 19.332640339102294 28.55732196109259 32.400662831143606 19.709374868661513 30-31 19.839919413744177 29.04918164381529 31.765278475434318 19.345620467006217 32-33 19.282571235631146 28.745760173994526 32.29204463434216 19.679623956032163 34-35 19.6021283355584 28.490786136277425 32.1562161433871 19.750869384777072 36-37 19.935429100325106 28.85625004983261 31.493878258157377 19.714442591684907 38-39 19.748261196661034 28.945252780051494 31.658917104619288 19.64756891866818 40-41 19.725051025213883 28.581890282310013 31.72992603962311 19.963132652852998 42-43 19.67638737026122 28.603411212749364 31.575522654546294 20.144678762443117 44-45 19.960483922952772 28.81294466735669 31.1819632845494 20.044608125141135 46-47 20.10608974086097 29.01846448532948 30.7419160020179 20.133529771791647 48-49 20.045405446896815 28.943374344050827 30.502428925860293 20.508791283192064 50-51 20.172003924985265 28.90537317833939 30.24000195681678 20.68262093985856 52-53 20.20869423967465 29.03011349131926 30.12890395421589 20.6322883147902 54-55 20.359516433814928 29.415557747514686 29.94002653865193 20.284899280018454 56-57 20.313825843035996 29.0057749068789 29.94467532990539 20.735723920179712 58-59 20.10660327012734 28.681562258734157 30.339687447168988 20.87214702396952 60-61 20.249594142955466 29.07879066019998 29.983021100782416 20.688594096062136 62-63 20.040135014952487 29.04114085661817 30.083280933077273 20.835443195352074 64-65 20.12227942667971 28.80387682162683 30.18031093656522 20.89353281512825 66-67 19.985619829148813 28.83745893286186 30.061901898882574 21.11501933910675 68-69 20.5445829216653 29.02875534154899 29.84446955332278 20.582192183462926 70-71 20.45133006107079 28.80339707718061 29.78658939943158 20.958683462317023 72-73 20.176585146598416 28.827391056455383 30.029488742424938 20.96653505452127 74-75 20.352766226747768 28.88969026482299 29.75151399914565 21.006029509283596 76-77 20.366462592838996 28.96300332456144 29.596590327838435 21.073943754761125 78-79 20.489263657141915 28.92179260093519 29.785102867344715 20.80384087457818 80-81 20.315846815530993 28.796833626180746 29.793021609625615 21.094297948662646 82-83 20.126970934730682 28.88982360830977 29.997168813705915 20.986036643253634 84-85 20.05387618814921 28.724446413281324 29.846748752389274 21.37492864618019 86-87 20.21793750479747 28.90201758549224 29.664078556316063 21.21596635339423 88-89 20.067215979613305 28.672301963511227 29.74578053775892 21.514701519116546 90-91 19.862120290914064 28.592082852965472 29.820509206373057 21.7252876497474 92-93 19.9975296467726 28.834569647372614 29.610649763035962 21.557250942818822 94-95 19.717959447692262 28.497193453050418 29.89123350966583 21.89361358959149 96-97 19.699974005792512 28.679949006216514 29.786449510863612 21.833627477127358 98-99 19.71448784644211 28.610292194870457 29.663147823184367 22.012072135503065 100 19.779501016384042 28.56908100557829 29.617533645001014 22.033884333036656 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 658.0 1 448.0 2 346.5 3 456.0 4 316.5 5 245.0 6 612.5 7 1577.5 8 2748.5 9 3424.0 10 3104.5 11 2573.5 12 2844.5 13 3536.5 14 4220.5 15 4884.5 16 5652.5 17 6626.0 18 7878.0 19 9604.5 20 12016.0 21 15288.0 22 20524.0 23 28663.5 24 38656.0 25 51260.5 26 68653.0 27 91068.0 28 119774.0 29 149223.0 30 175228.5 31 200727.0 32 226620.5 33 250427.5 34 270583.0 35 286667.5 36 295885.5 37 302478.5 38 307210.0 39 305358.5 40 302111.5 41 295668.5 42 285159.0 43 276867.5 44 264365.0 45 251463.5 46 241369.0 47 230620.0 48 220551.5 49 210789.0 50 201829.5 51 185121.5 52 163335.5 53 146102.0 54 133907.0 55 124617.0 56 111098.5 57 96696.5 58 85231.5 59 73550.5 60 59336.0 61 44578.0 62 33359.0 63 24919.0 64 18229.0 65 13080.0 66 9241.5 67 6195.5 68 4181.0 69 2798.0 70 1850.5 71 1226.5 72 825.5 73 580.5 74 394.5 75 279.0 76 178.0 77 122.0 78 83.0 79 47.5 80 30.5 81 17.0 82 8.0 83 3.5 84 4.5 85 3.0 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1373555648261102 2 0.0 3 0.0 4 0.0 5 0.014892348724751422 6 2.5676463318536935E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.027089209358179E-4 82-83 6.486685469946173E-4 84-85 3.919039138092479E-4 86-87 5.000153383083508E-4 88-89 6.013697987762598E-4 90-91 8.378635398680474E-4 92-93 2.90549453341339E-4 94-95 2.90549453341339E-4 96-97 6.419115829634233E-4 98-99 0.0011892256694901317 100 1.4865320868626646E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7399773.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.86121401091218 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0285113858698 45.05465074364671 2 16.239866796677667 19.767560172426123 3 5.0797026720907725 9.27470614313757 4 2.06024419846088 5.015558523090714 5 0.9357626976299112 2.847582690194126 6 0.4923652511096658 1.7979568151593133 7 0.3069800862928086 1.3078226510268438 8 0.17327934128560862 0.8436792858922654 9 0.12443218168515945 0.6815784275446677 >10 0.4825290542983352 5.125323349048796 >50 0.03850894540456087 1.6377377893590048 >100 0.034175264571761656 4.182965760874911 >500 0.002731685045493919 1.1314454606358635 >1k 8.216162077565205E-4 0.6817648659301875 >5k 4.441168496075023E-5 0.1898281126931858 >10k+ 4.441168496075023E-5 0.45983920933971567 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17156 0.2318449498383261 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16869 0.2279664524844208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8473 0.11450351247261233 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.351392806238786E-5 0.0 6 0.0 0.0 0.0 1.351392806238786E-5 0.0 7 0.0 0.0 0.0 1.351392806238786E-5 0.0 8 0.0 0.0 0.0 1.351392806238786E-5 0.0 9 6.75696403119393E-5 0.0 0.0 6.75696403119393E-5 0.0 10-11 6.75696403119393E-5 0.0 0.0 1.2162535256149073E-4 0.0 12-13 6.75696403119393E-5 0.0 0.0 1.4865320868626646E-4 0.0 14-15 6.75696403119393E-5 0.0 0.0 2.6352159721656327E-4 0.0 16-17 8.108356837432716E-5 0.0 0.0 3.648760576844722E-4 0.0 18-19 9.459749643671502E-5 0.0 0.0 4.054178418716358E-4 0.0 20-21 1.1486838853029681E-4 0.0 0.0 4.324456979964115E-4 0.0 22-23 1.2162535256149073E-4 0.0 0.0 5.81098906682678E-4 0.0 24-25 1.351392806238786E-4 0.0 0.0 8.513774679304352E-4 0.0 26-27 1.351392806238786E-4 0.0 0.0 0.0014054485184883374 0.0 28-29 1.351392806238786E-4 0.0 0.0 0.0033379402314098012 0.0 30-31 1.351392806238786E-4 0.0 0.0 0.009831382665387169 0.0 32-33 1.351392806238786E-4 0.0 0.0 0.02522374672844694 0.0 34-35 1.4865320868626646E-4 0.0 0.0 0.05149482288172894 0.0 36-37 1.4865320868626646E-4 0.0 0.0 0.09132036888158596 0.0 38-39 1.4865320868626646E-4 0.0 0.0 0.14450443277111338 0.0 40-41 1.4865320868626646E-4 0.0 0.0 0.20016154549605777 0.0 42-43 1.4865320868626646E-4 0.0 0.0 0.25854847168960454 0.0 44-45 1.4865320868626646E-4 0.0 0.0 0.3351859577314061 6.75696403119393E-6 46-47 1.7568106481104218E-4 0.0 0.0 0.42851990189428785 1.351392806238786E-5 48-49 1.8919499287343004E-4 0.0 0.0 0.5277797035125267 1.351392806238786E-5 50-51 1.95951956904624E-4 0.0 0.0 0.6278300699224152 1.351392806238786E-5 52-53 2.027089209358179E-4 0.0 0.0 0.7282250414978946 1.351392806238786E-5 54-55 2.027089209358179E-4 0.0 0.0 0.8438839948198411 1.351392806238786E-5 56-57 2.1622284899820574E-4 0.0 0.0 0.9845166872010804 1.351392806238786E-5 58-59 2.297367770605936E-4 0.0 0.0 1.1409268905951575 1.351392806238786E-5 60-61 2.4325070512298146E-4 0.0 0.0 1.2998439276448075 1.351392806238786E-5 62-63 2.4325070512298146E-4 0.0 0.0 1.4644503284087227 1.351392806238786E-5 64-65 2.4325070512298146E-4 0.0 0.0 1.6445234198400411 1.351392806238786E-5 66-67 2.4325070512298146E-4 0.0 0.0 1.854523104965517 1.351392806238786E-5 68-69 2.4325070512298146E-4 0.0 0.0 2.093564221497065 1.351392806238786E-5 70-71 2.4325070512298146E-4 0.0 0.0 2.349768026667845 1.351392806238786E-5 72-73 2.4325070512298146E-4 0.0 0.0 2.611053068790083 1.351392806238786E-5 74-75 2.4325070512298146E-4 0.0 0.0 2.879095074943515 1.351392806238786E-5 76-77 2.4325070512298146E-4 0.0 0.0 3.171570263033744 1.351392806238786E-5 78-79 2.4325070512298146E-4 0.0 0.0 3.505519696347442 1.351392806238786E-5 80-81 2.4325070512298146E-4 0.0 0.0 3.8684159635707744 2.702785612477572E-5 82-83 2.567646331853693E-4 0.0 0.0 4.238197577141893 2.702785612477572E-5 84-85 2.837924893101451E-4 0.0 0.0 4.615303739722827 2.702785612477572E-5 86-87 2.90549453341339E-4 0.0 0.0 5.016984980485212 2.702785612477572E-5 88 2.973064173725329E-4 0.0 0.0 5.340271924557686 2.702785612477572E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13595 0.0 58.505177 1 GTATCAA 25240 0.0 40.332386 1 ATCAACG 30035 0.0 33.428288 3 TCAACGC 30500 0.0 32.949463 4 TATCAAC 31975 0.0 31.56182 2 CAACGCA 32180 0.0 31.287724 5 GTGGTAT 5430 0.0 30.942482 1 TGGTATC 5310 0.0 30.097118 2 AACGCAG 33695 0.0 29.947065 6 CGCAGAG 36440 0.0 27.394531 8 ACGCAGA 37265 0.0 26.914171 7 GCAGAGT 40110 0.0 24.53645 9 GTACATG 19900 0.0 21.805428 1 GAGTACT 30045 0.0 21.704435 12-13 CAGAGTA 40815 0.0 21.245369 10-11 TACATGG 19595 0.0 20.893606 2 GGACCGT 365 0.0 20.602259 6 AGAGTAC 38725 0.0 20.419792 10-11 ACATGGG 19620 0.0 20.292006 3 GTACTTT 32945 0.0 19.71543 14-15 >>END_MODULE