FastQCFastQC Report
Wed 25 May 2016
SRR2049465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8973773
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89950.042.7148741
GTATCAA153600.035.9141041
TCAACGC165550.032.30694
ATCAACG165950.032.1449623
CAACGCA179450.029.8836785
AACGCAG188750.028.5110246
TATCAAC191050.028.2417492
ACGCAGA212550.024.8764347
CGCAGAG215400.024.1313698
GTACATG254650.021.6811661
TACATGG256600.020.5326822
GCAGAGT247750.020.1459719
GTGGTAT54950.019.4274771
ACATGGG262650.018.8606873
AGAGTAC216850.017.86949310-11
TGGTATC57400.016.7037892
CAGAGTA240650.016.62952210-11
CATGGGG173250.015.3541364
GAGTACA202050.014.6868521
GAGTACT96100.013.86420312-13
Produced by FastQC (version 0.11.5)