##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049465_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8973773
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.056509564037334	31.0	31.0	34.0	28.0	34.0
2	31.16428708415067	31.0	31.0	34.0	28.0	34.0
3	31.354108466973702	33.0	31.0	34.0	28.0	34.0
4	34.944919266399985	37.0	35.0	37.0	32.0	37.0
5	34.934044019165626	37.0	35.0	37.0	32.0	37.0
6	34.91903862511343	37.0	35.0	37.0	32.0	37.0
7	34.87819170375716	37.0	35.0	37.0	32.0	37.0
8	34.856463942201344	37.0	35.0	37.0	32.0	37.0
9	36.436031978968046	39.0	37.0	39.0	32.0	39.0
10-11	36.37443910159082	39.0	37.0	39.0	32.0	39.0
12-13	36.29721238769913	39.0	37.0	39.0	32.0	39.0
14-15	37.554753613669526	40.0	38.0	41.0	32.0	41.0
16-17	37.5488591587953	40.0	38.0	41.0	32.0	41.0
18-19	37.57140407942122	40.0	38.0	41.0	32.0	41.0
20-21	37.62787536524492	40.0	38.0	41.0	32.0	41.0
22-23	37.59477741413784	40.0	38.0	41.0	32.0	41.0
24-25	37.60245099803616	40.0	38.0	41.0	32.0	41.0
26-27	37.60797632166537	40.0	38.0	41.0	32.0	41.0
28-29	37.590974498686336	40.0	38.0	41.0	32.0	41.0
30-31	37.5344321167919	40.0	38.0	41.0	32.0	41.0
32-33	37.49122442700523	40.0	38.0	41.0	32.0	41.0
34-35	37.42102586058283	40.0	38.0	41.0	32.0	41.0
36-37	37.30632850864402	40.0	38.0	41.0	31.0	41.0
38-39	37.19914889757073	40.0	37.0	41.0	31.0	41.0
40-41	37.0776853281223	40.0	37.0	41.0	31.0	41.0
42-43	36.98449481617153	40.0	37.0	41.0	30.5	41.0
44-45	36.82960244258463	40.0	37.0	41.0	30.0	41.0
46-47	36.71112189933933	40.0	37.0	41.0	30.0	41.0
48-49	36.61242160906009	40.0	36.0	41.0	30.0	41.0
50-51	36.49986488403484	40.0	36.0	41.0	30.0	41.0
52-53	36.385965802789975	40.0	36.0	41.0	30.0	41.0
54-55	36.24018057956224	39.0	35.0	41.0	30.0	41.0
56-57	36.07489965480518	39.0	35.0	41.0	29.5	41.0
58-59	35.89283938873872	39.0	35.0	41.0	29.0	41.0
60-61	35.69712299386222	39.0	35.0	41.0	29.0	41.0
62-63	35.442540835387746	38.5	35.0	40.0	28.0	41.0
64-65	35.200896657403746	38.0	35.0	40.0	28.0	41.0
66-67	34.97118581002662	37.5	34.0	40.0	28.0	41.0
68-69	34.68193189197008	37.0	34.0	40.0	28.0	41.0
70-71	34.362768815302104	36.5	34.0	39.5	28.0	41.0
72-73	34.020697369991424	36.0	34.0	39.0	27.0	41.0
74-75	33.71201338611975	36.0	34.0	39.0	26.5	40.5
76-77	33.377462133263236	35.0	34.0	38.5	26.0	40.0
78-79	33.02300693364987	35.0	33.0	37.0	26.0	39.0
80-81	32.67697929287937	35.0	33.0	37.0	26.0	39.0
82-83	32.36461993188372	35.0	33.0	36.0	25.5	39.0
84-85	32.06633525274152	35.0	33.0	36.0	25.0	37.0
86-87	31.78049662054077	35.0	33.0	36.0	25.0	37.0
88-89	31.534731433478427	35.0	33.0	35.0	24.0	37.0
90-91	31.302761057138397	35.0	33.0	35.0	23.5	36.0
92-93	31.07545271091658	35.0	32.0	35.0	22.0	36.0
94-95	30.851479973919552	35.0	32.0	35.0	20.0	36.0
96-97	30.63851971740315	35.0	32.0	35.0	17.5	36.0
98-99	30.440853139476562	35.0	32.0	35.0	4.5	35.0
100	30.297411356404936	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33005896014776326
1101	2	0.34455880835479746
1101	3	0.41821843458420815
1101	4	0.44411567824393217
1101	5	0.4050594148005189
1101	6	0.4252712774249332
1101	7	0.4954303149901733
1101	8	0.4998389729349739
1101	9	0.5997785086653309
1101	10-11	0.6174658231371666
1101	12-13	0.6113095027250779
1101	14-15	0.7173961293836442
1101	16-17	0.6528843127541251
1101	18-19	0.6732830203024633
1101	20-21	0.6510900216891287
1101	22-23	0.6015659479621505
1101	24-25	0.6704836063899009
1101	26-27	0.6464397683153891
1101	28-29	0.6610364200693297
1101	30-31	0.6201995598696399
1101	32-33	0.6082360627330203
1101	34-35	0.6350445620266925
1101	36-37	0.6467619655075012
1101	38-39	0.6373901949509317
1101	40-41	0.6569642001220686
1101	42-43	0.6227293878942461
1101	44-45	0.6179809339390729
1101	46-47	0.5780977986391278
1101	48-49	0.5726281798187358
1101	50-51	0.5332751301360901
1101	52-53	0.5735806352668718
1101	54-55	0.5144923423108807
1101	56-57	0.5322866136543851
1101	58-59	0.5673528852540386
1101	60-61	0.5863295739384142
1101	62-63	0.5438572128014698
1101	64-65	0.5991249311773217
1101	66-67	0.5770081822886155
1101	68-69	0.506730175761362
1101	70-71	0.5537598725950872
1101	72-73	0.5687759406674076
1101	74-75	0.4982014325802382
1101	76-77	0.523683030657125
1101	78-79	0.5132998273825962
1101	80-81	0.5448872367344322
1101	82-83	0.4941266934184583
1101	84-85	0.4725737142515385
1101	86-87	0.43264465757629367
1101	88-89	0.4111584100906889
1101	90-91	0.4403122396979491
1101	92-93	0.4575493732394271
1101	94-95	0.46455083793057383
1101	96-97	0.47311588062487786
1101	98-99	0.5609046851560855
1101	100	0.5220893595615834
1102	1	0.27547964885545184
1102	2	0.29529129492173567
1102	3	0.40186402916928543
1102	4	0.41842324000038644
1102	5	0.37984735064315345
1102	6	0.3159032553671679
1102	7	0.3782528663227822
1102	8	0.440518524893335
1102	9	0.5254186892933106
1102	10-11	0.5144731201850874
1102	12-13	0.49621872534266487
1102	14-15	0.5858738304118916
1102	16-17	0.5121707585734683
1102	18-19	0.554914578423876
1102	20-21	0.5532173657774209
1102	22-23	0.566344332810111
1102	24-25	0.5505213143328476
1102	26-27	0.5142201771879442
1102	28-29	0.5439445180553335
1102	30-31	0.4963514379291496
1102	32-33	0.49173380105123954
1102	34-35	0.5218047147574509
1102	36-37	0.5901446923643761
1102	38-39	0.5492237914883589
1102	40-41	0.538221751539119
1102	42-43	0.4933566197890471
1102	44-45	0.48341188611626507
1102	46-47	0.43279139685414236
1102	48-49	0.41473472162915215
1102	50-51	0.4517917227805981
1102	52-53	0.4495081866541213
1102	54-55	0.4567050457085955
1102	56-57	0.4326406973202239
1102	58-59	0.4571758459048496
1102	60-61	0.4192322254659828
1102	62-63	0.3556043898328767
1102	64-65	0.37885031400125513
1102	66-67	0.4393497613116537
1102	68-69	0.3916260643132645
1102	70-71	0.2749476816683014
1102	72-73	0.36755435503623346
1102	74-75	0.39194715613461995
1102	76-77	0.3549307678044329
1102	78-79	0.3684922360343137
1102	80-81	0.3914117966073718
1102	82-83	0.3798496524087156
1102	84-85	0.36024888619285633
1102	86-87	0.3612820544430839
1102	88-89	0.32518259350157663
1102	90-91	0.32119989320469244
1102	92-93	0.30122849742904023
1102	94-95	0.3192273481039898
1102	96-97	0.3163619599199201
1102	98-99	0.3292761465370404
1102	100	0.3294128059584267
1103	1	0.24542548309767653
1103	2	0.24620115812005494
1103	3	0.28726553386799125
1103	4	0.3065290228943809
1103	5	0.29547534732279246
1103	6	0.2625628032341325
1103	7	0.2678444637769388
1103	8	0.32070492497276604
1103	9	0.4205697820924854
1103	10-11	0.4318732470256208
1103	12-13	0.4304826162545723
1103	14-15	0.47393699311649584
1103	16-17	0.4329414874219921
1103	18-19	0.4182751236509148
1103	20-21	0.42188795308545224
1103	22-23	0.4816788736148183
1103	24-25	0.4736162661891825
1103	26-27	0.48550250097944314
1103	28-29	0.44995943404789074
1103	30-31	0.4430711043548641
1103	32-33	0.4871088260027747
1103	34-35	0.4395732267320227
1103	36-37	0.4606147059473429
1103	38-39	0.47069914930484913
1103	40-41	0.5420703559829292
1103	42-43	0.4862964790511555
1103	44-45	0.4452292966213278
1103	46-47	0.4075603420433751
1103	48-49	0.4173529407300123
1103	50-51	0.4162207657519943
1103	52-53	0.3899630442668851
1103	54-55	0.38884138656032263
1103	56-57	0.32523478007968265
1103	58-59	0.36005987245042803
1103	60-61	0.37161223689914635
1103	62-63	0.3825609516324633
1103	64-65	0.42798390984032864
1103	66-67	0.33184172607050755
1103	68-69	0.33888464360606463
1103	70-71	0.41472992410832177
1103	72-73	0.3799728420785655
1103	74-75	0.36197115087846754
1103	76-77	0.302549417569864
1103	78-79	0.3163773878106042
1103	80-81	0.33942186627302817
1103	82-83	0.33467993550616626
1103	84-85	0.31309985373859917
1103	86-87	0.2991058686467021
1103	88-89	0.27052402259054986
1103	90-91	0.2665093051430496
1103	92-93	0.21412615793899548
1103	94-95	0.23171028887155742
1103	96-97	0.2598635537479055
1103	98-99	0.23738812549792598
1103	100	0.23692650510129454
1104	1	0.13297288877092583
1104	2	0.16359109623990165
1104	3	0.22334874409095207
1104	4	0.23713700293699702
1104	5	0.22062519381840673
1104	6	0.20165669183256796
1104	7	0.24571898694065197
1104	8	0.26077574861816544
1104	9	0.33874803955863086
1104	10-11	0.33952469883220715
1104	12-13	0.34466180853998907
1104	14-15	0.3978931439051081
1104	16-17	0.3654960884843419
1104	18-19	0.3575535364480231
1104	20-21	0.38022500148988314
1104	22-23	0.3634964611140319
1104	24-25	0.4107258589125635
1104	26-27	0.41200427757441815
1104	28-29	0.3559672202270221
1104	30-31	0.39213917900647743
1104	32-33	0.41019278800746406
1104	34-35	0.4032645804670878
1104	36-37	0.36454952692284337
1104	38-39	0.37335239940279763
1104	40-41	0.3470082493333564
1104	42-43	0.38151136263935115
1104	44-45	0.3395476806610205
1104	46-47	0.3170451457030552
1104	48-49	0.24603652029362166
1104	50-51	0.26334144110903424
1104	52-53	0.2218181763795073
1104	54-55	0.2137724965308152
1104	56-57	0.20821734017950178
1104	58-59	0.2534795873019675
1104	60-61	0.24641117038942895
1104	62-63	0.23147551872010297
1104	64-65	0.2521021260394889
1104	66-67	0.24252391668355244
1104	68-69	0.25733642179252314
1104	70-71	0.25107468867891924
1104	72-73	0.2092531456070219
1104	74-75	0.2375140738157242
1104	76-77	0.2108711704912949
1104	78-79	0.1972907883119177
1104	80-81	0.22520024371421243
1104	82-83	0.2573421487889149
1104	84-85	0.1947931237451428
1104	86-87	0.2126409638544935
1104	88-89	0.1614440520874858
1104	90-91	0.17737566099533453
1104	92-93	0.2061590410355727
1104	94-95	0.24160423955408916
1104	96-97	0.26640736163130185
1104	98-99	0.24765049878128664
1104	100	0.21776943837531348
1105	1	0.19401387311310714
1105	2	0.15221534741013087
1105	3	0.23323766218928554
1105	4	0.2068350847194651
1105	5	0.20272763002266458
1105	6	0.23270203763450326
1105	7	0.2693923905220572
1105	8	0.26326916076120455
1105	9	0.2875763806527658
1105	10-11	0.3377646644048298
1105	12-13	0.29790886308841635
1105	14-15	0.39540301247910037
1105	16-17	0.33973743794528133
1105	18-19	0.3590030146582279
1105	20-21	0.3475862169265085
1105	22-23	0.3271808541810728
1105	24-25	0.30569614093142405
1105	26-27	0.3634186056814315
1105	28-29	0.36694902031184995
1105	30-31	0.3775361698986117
1105	32-33	0.36089278732932684
1105	34-35	0.3836203769778308
1105	36-37	0.33220692634794347
1105	38-39	0.33739264335373775
1105	40-41	0.36181699972046033
1105	42-43	0.35546034034165785
1105	44-45	0.3657643486218447
1105	46-47	0.30848351590638146
1105	48-49	0.28697935254701434
1105	50-51	0.2819329132119748
1105	52-53	0.3035079325941368
1105	54-55	0.25608571238922906
1105	56-57	0.23624977199175135
1105	58-59	0.2476416949225566
1105	60-61	0.2166618605466013
1105	62-63	0.245535828807121
1105	64-65	0.26255439884631926
1105	66-67	0.255016676348788
1105	68-69	0.22614007747657183
1105	70-71	0.24837406901439607
1105	72-73	0.2680511916926065
1105	74-75	0.26978445585063326
1105	76-77	0.23616962868258895
1105	78-79	0.2097206828408389
1105	80-81	0.25493586862940987
1105	82-83	0.24704220022347556
1105	84-85	0.1831202989628622
1105	86-87	0.18108284870156766
1105	88-89	0.15133778801346764
1105	90-91	0.15865672051176105
1105	92-93	0.13431921003953917
1105	94-95	0.13472018333176194
1105	96-97	0.13415390247564574
1105	98-99	0.0650552616126312
1105	100	0.041976175355220136
1106	1	0.22868041429723363
1106	2	0.2562207233520404
1106	3	0.24830362141904416
1106	4	0.2610170115714965
1106	5	0.26871009193595796
1106	6	0.2616319351378138
1106	7	0.2914892224983774
1106	8	0.26295627060407156
1106	9	0.3362263305630435
1106	10-11	0.3272052806073589
1106	12-13	0.3207826659836357
1106	14-15	0.4244697137553928
1106	16-17	0.3903716927829137
1106	18-19	0.38945633763147214
1106	20-21	0.3773721221264523
1106	22-23	0.35877490763024156
1106	24-25	0.37959524106673825
1106	26-27	0.38143945962821846
1106	28-29	0.3495836986003269
1106	30-31	0.34312459078602586
1106	32-33	0.38007131845560593
1106	34-35	0.3701063064163037
1106	36-37	0.35741740317616433
1106	38-39	0.38846419695219225
1106	40-41	0.47885756272451374
1106	42-43	0.4176713142536528
1106	44-45	0.34012515768336726
1106	46-47	0.3531904576100544
1106	48-49	0.3617910792394383
1106	50-51	0.3951060281647685
1106	52-53	0.353051260230572
1106	54-55	0.29462384896859106
1106	56-57	0.31447212675455205
1106	58-59	0.34100683143291377
1106	60-61	0.31435366972852563
1106	62-63	0.3775416842241768
1106	64-65	0.3464224691163409
1106	66-67	0.4022437147165121
1106	68-69	0.3241634662237729
1106	70-71	0.35708453640565097
1106	72-73	0.31606161296047475
1106	74-75	0.3442586484931027
1106	76-77	0.27999508405597595
1106	78-79	0.26362499246194915
1106	80-81	0.2878075881286435
1106	82-83	0.2906040356518744
1106	84-85	0.270215635493642
1106	86-87	0.26728365644249763
1106	88-89	0.29295156325481386
1106	90-91	0.32341903968448804
1106	92-93	0.2262110260329493
1106	94-95	0.289026163802955
1106	96-97	0.3573853657547872
1106	98-99	0.35473734754576114
1106	100	0.34005643660696094
1107	1	0.169589492731145
1107	2	0.15025053031460445
1107	3	0.23105775411115914
1107	4	0.16724453221538482
1107	5	0.1705671015897181
1107	6	0.17851442311988563
1107	7	0.18356748165744818
1107	8	0.12381319558202364
1107	9	0.15401591371153245
1107	10-11	0.1571670048496756
1107	12-13	0.18548988792080223
1107	14-15	0.2355814040193991
1107	16-17	0.19115650632607384
1107	18-19	0.17971793299739147
1107	20-21	0.22683077063944523
1107	22-23	0.19241161572475818
1107	24-25	0.21212159157381194
1107	26-27	0.25849080632443844
1107	28-29	0.19536117117427665
1107	30-31	0.18735442408716807
1107	32-33	0.18781198265584464
1107	34-35	0.1849297956101097
1107	36-37	0.19822069534620113
1107	38-39	0.20174955715370402
1107	40-41	0.24406705457611366
1107	42-43	0.25194769135126904
1107	44-45	0.20026471730979978
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2216	90-91	-0.9039697315081376
2216	92-93	-0.9168858746638335
2216	94-95	-0.8851461598833161
2216	96-97	-0.9171055314303729
2216	98-99	-0.9250504950689198
2216	100	-0.9829601186700607
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118885.0
3	23856.0
4	14505.0
5	11903.0
6	10392.0
7	11762.0
8	13216.0
9	15039.0
10	16890.0
11	18736.0
12	20877.0
13	22728.0
14	25461.0
15	26209.0
16	26430.0
17	27098.0
18	25898.0
19	24180.0
20	24410.0
21	26137.0
22	29991.0
23	36131.0
24	44314.0
25	55162.0
26	65783.0
27	74962.0
28	91466.0
29	116799.0
30	141477.0
31	166475.0
32	213603.0
33	285210.0
34	397030.0
35	579760.0
36	978440.0
37	1777460.0
38	2451340.0
39	961532.0
40	2226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.548927671720456	20.75073183880487	14.793755228316199	22.906585261158472
2	15.404644828231854	20.85729156450333	40.26516401116292	23.472899596101893
3	17.994332707770436	25.858592320756614	30.689678078452676	25.457396893020274
4	12.774797211067773	17.80248492296623	39.04324570173725	30.379472164228748
5	13.06907560726975	37.75716164226376	35.49063865255708	13.683124097909408
6	29.838560395726017	38.26018655584924	18.351920974265575	13.549332074159176
7	26.21053749689447	32.77277964329306	23.19364130441446	17.823041555398003
8	24.22287614230147	36.263629103386265	21.207026406419153	18.30646834789311
9	24.45618606392855	17.682191683114468	22.168234548074576	35.693387704882404
10-11	23.253021830425087	27.420087633319163	29.38795601070181	19.938934525553943
12-13	23.88296656431157	25.16280278129754	29.074979055118057	21.879251599272834
14-15	21.527323125887992	27.431269032806206	26.330958367146064	24.71044947415974
16-17	21.002528980349428	30.505327420286797	26.95817377560556	21.533969823758216
18-19	20.936536283723644	29.48714712482772	28.853222662491657	20.723093928956974
20-21	22.115486529045832	28.806622705064022	28.256744170287813	20.82114659560233
22-23	21.82815141939382	28.713422644432846	28.20170662283475	21.256719313338586
24-25	21.567667743721984	28.795488739067327	28.246515972543506	21.390327544667183
26-27	21.337212940401553	28.737528690148956	28.32710305318182	21.598155316267675
28-29	21.374459655453364	28.728517380882074	28.48468701740398	21.412335946260583
30-31	21.718484945444562	29.07873563199176	28.019088580943357	21.183690841620322
32-33	21.339916121223172	28.9148012589228	28.327094797528673	21.418187822325354
34-35	21.486514077035416	28.585229633367632	28.404847835351553	21.5234084542454
36-37	21.796682329422968	28.869976238668286	27.939806558096347	21.3935348738124
38-39	21.58827342909756	28.959221524787033	28.09932411394761	21.353180932167803
40-41	21.50832715153374	28.671877507641064	28.282975814303494	21.536819526521704
42-43	21.404116621199535	28.69130055164037	28.24057999554328	21.664002831616816
44-45	21.526713217966584	28.829376640030496	28.125344655447655	21.518565486555268
46-47	21.531943848843614	28.879442868714527	28.023148600944097	21.565464681497755
48-49	21.41519225844778	28.732083700559595	28.130078763589694	21.722645277402926
50-51	21.429703824146426	28.63841623767847	28.10735336132063	21.82452657685447
52-53	21.384429934616616	28.65551314204121	28.13508293816546	21.824973985176705
54-55	21.501819303609274	28.960484868262093	27.944565850231324	21.59312997789731
56-57	21.369235361347176	28.73253797579955	27.9976265891565	21.90060007369678
58-59	21.316053524377626	28.37683856879034	28.24124572243155	22.065862184400476
60-61	21.49402486675816	28.703028792786323	27.95921370666774	21.84373263378778
62-63	21.362957023681332	28.73606185987205	27.994405211563596	21.906575904883024
64-65	21.371145612292242	28.4655067916363	28.131616603109006	22.031730992962448
66-67	21.2740207526196	28.56177293555436	27.97187400599651	22.19233230582953
68-69	21.611008763470664	28.68346322124739	27.885896501337903	21.819631513944042
70-71	21.603531089843393	28.467803974064452	27.976286183825717	21.95237875226644
72-73	21.412078260153802	28.58727249027003	28.10487726870233	21.895771980873832
74-75	21.44501252974163	28.569750306876408	27.995599125398062	21.9896380379839
76-77	21.509022406523766	28.609565817704297	27.819471738957567	22.061940036814367
78-79	21.58107185564109	28.6401744671596	27.91854595691828	21.860207720281032
80-81	21.471444385018422	28.512591954058543	27.978220316799952	22.037743344123083
82-83	21.40921291421121	28.571208059102972	28.063474051005105	21.956104975680717
84-85	21.45450735902174	28.406063530770137	27.980392659478902	22.159036450729218
86-87	21.621660028645223	28.549588831650823	27.8539883828634	21.974762756840548
88-89	21.490785261835583	28.40201024515515	27.914584363931432	22.19262012907783
90-91	21.470801177127772	28.320568930228745	27.909216182876545	22.29941370976694
92-93	21.632012271502994	28.43614788569778	27.74870098954818	22.183138853251048
94-95	21.53973245691857	28.219209274042186	27.899681821140415	22.341376447898824
96-97	21.62123952233722	28.216143796578883	27.867616987297993	22.294999693785904
98-99	21.585251164612995	28.30049001817208	27.75110373476909	22.363155082445836
100	21.70945080217259	28.19719763661686	27.71102061067401	22.382330950536545
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4867.0
1	3723.5
2	2996.5
3	3481.5
4	3296.0
5	2819.0
6	2546.0
7	2446.5
8	2365.0
9	2353.0
10	2221.5
11	2029.0
12	2005.0
13	2075.5
14	2267.5
15	2521.5
16	2952.5
17	3669.0
18	4670.0
19	6185.0
20	8608.5
21	12124.0
22	17467.0
23	25514.0
24	36562.5
25	50942.0
26	69115.5
27	90645.5
28	114862.0
29	141798.5
30	168139.0
31	191060.5
32	212044.5
33	232519.0
34	251792.0
35	267309.5
36	281341.0
37	297030.0
38	309764.0
39	315827.5
40	320916.0
41	324502.5
42	323114.0
43	321017.5
44	316333.0
45	309902.0
46	304001.5
47	295415.0
48	287520.5
49	280866.0
50	272202.5
51	258942.0
52	240491.5
53	225309.5
54	215596.0
55	205667.5
56	190252.5
57	171218.0
58	154166.5
59	138978.5
60	122148.0
61	103235.0
62	86572.5
63	72654.5
64	60207.5
65	49086.0
66	39288.0
67	31169.0
68	24551.0
69	19090.0
70	14470.5
71	10998.0
72	8459.0
73	6265.5
74	4596.5
75	3305.0
76	2338.5
77	1673.5
78	1158.0
79	756.0
80	467.5
81	293.0
82	203.5
83	158.0
84	125.0
85	95.0
86	83.0
87	62.0
88	42.5
89	34.5
90	25.5
91	22.5
92	20.0
93	16.5
94	18.5
95	18.0
96	11.0
97	15.0
98	17.0
99	18.5
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0777376472527219
2	0.013517168308135276
3	0.017684869006604025
4	0.011366456450369315
5	0.009650344398058654
6	0.007755934989663768
7	0.020838503492343743
8	0.009795210999877086
9	0.010508400424213984
10-11	0.014180211601073483
12-13	0.015757028843943345
14-15	0.010513972216591616
16-17	0.01319957614261025
18-19	0.009566767512394174
20-21	0.013884906605058988
22-23	0.01466495753792747
24-25	0.01638106959023813
26-27	0.005878240958401778
28-29	0.012720401998133894
30-31	0.010853851551627169
32-33	0.013166145388344456
34-35	0.009594626474282334
36-37	0.01116030013239693
38-39	0.019752003978705503
40-41	0.01333887095205105
42-43	0.008998444689875708
44-45	0.0217355620651425
46-47	0.01939540926653705
48-49	0.01861535833366857
50-51	0.011282879564704836
52-53	0.011188159094285091
54-55	0.012358235493587815
56-57	0.020894221416120067
58-59	0.0024181578918922955
60-61	0.007906373383859832
62-63	0.0166540874167421
64-65	0.025190073339274352
66-67	0.013584029816666857
68-69	0.015127416305270926
70-71	0.026505016340395503
72-73	0.02091093679325296
74-75	0.021395682730106946
76-77	0.010224239012954751
78-79	0.012051786912818052
80-81	0.020431762648776608
82-83	0.012943273693239176
84-85	0.012402809832608871
86-87	0.017790733061779033
88-89	0.015985472331426255
90-91	0.01587960827625125
92-93	0.008792288371903323
94-95	0.016280777327440755
96-97	0.014403083296178764
98-99	0.010502828631836353
100	0.007845083667705881
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8973773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.67771709421393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54380426781627	43.153825408591665
2	15.59969879502677	18.307094252990616
3	5.011851048341087	8.822519337986932
4	2.087783630455723	4.900255088872472
5	1.048233713164987	3.075398063485625
6	0.6496724337760427	2.2872777163812037
7	0.4363455424712593	1.7922632207514901
8	0.29279883815450825	1.3744613912595813
9	0.21318372846728906	1.1258221057283977
>10	1.0432679219785053	10.686643575594363
>50	0.05354582532891031	2.127671054320576
>100	0.019015458887251622	1.9323797039244552
>500	7.037615416838778E-4	0.2761275132827428
>1k	7.602767496610424E-5	0.05950032639889917
>5k	1.9006914679126647E-5	0.07876124043096877
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1143584755264035E-5	0.0
2	0.0	0.0	0.0	1.1143584755264035E-5	0.0
3	0.0	0.0	0.0	1.1143584755264035E-5	0.0
4	0.0	0.0	0.0	3.34307542657921E-5	0.0
5	0.0	0.0	0.0	3.34307542657921E-5	0.0
6	0.0	0.0	0.0	3.34307542657921E-5	0.0
7	0.0	0.0	0.0	3.34307542657921E-5	0.0
8	0.0	0.0	0.0	4.457433902105614E-5	0.0
9	0.0	0.0	0.0	5.571792377632017E-5	0.0
10-11	0.0	0.0	0.0	7.243330090921622E-5	0.0
12-13	0.0	0.0	0.0	1.1143584755264034E-4	0.0
14-15	0.0	0.0	0.0	1.2815122468553638E-4	0.0
16-17	0.0	0.0	0.0	1.3929480944080042E-4	0.0
18-19	0.0	0.0	0.0	1.894409408394886E-4	0.0
20-21	0.0	0.0	0.0	2.284434874829127E-4	0.0
22-23	0.0	0.0	0.0	5.627510301408337E-4	0.0
24-25	0.0	5.571792377632017E-6	0.0	0.0011255020602816675	0.0
26-27	0.0	1.1143584755264035E-5	0.0	0.002323437421472551	0.0
28-29	0.0	1.1143584755264035E-5	0.0	0.0058838127507794095	0.0
30-31	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.015545300733593328	0.0
32-33	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.03952629512692153	0.0
34-35	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.07910830817761938	0.0
36-37	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.14006371678891366	0.0
38-39	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.21860370214401456	0.0
40-41	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.29983486321751174	0.0
42-43	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.3849885661248619	0.0
44-45	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.49530448340959815	0.0
46-47	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.6304483075290628	0.0
48-49	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.7727407412690293	0.0
50-51	1.1143584755264035E-5	1.1143584755264035E-5	0.0	0.9164539820652918	0.0
52-53	1.1143584755264035E-5	1.671537713289605E-5	0.0	1.0607634046459609	0.0
54-55	1.1143584755264035E-5	2.228716951052807E-5	0.0	1.227031260986878	0.0
56-57	1.1143584755264035E-5	2.228716951052807E-5	0.0	1.423314362866099	0.0
58-59	1.1143584755264035E-5	2.228716951052807E-5	0.0	1.6433890181978081	0.0
60-61	1.1143584755264035E-5	2.228716951052807E-5	0.0	1.872239246524288	0.0
62-63	1.1143584755264035E-5	2.7858961888160085E-5	0.0	2.102326412758602	0.0
64-65	1.1143584755264035E-5	3.34307542657921E-5	0.0	2.352934490319735	0.0
66-67	1.1143584755264035E-5	3.34307542657921E-5	0.0	2.6428571348974392	0.0
68-69	1.1143584755264035E-5	3.34307542657921E-5	0.0	2.970027211519614	0.0
70-71	1.1143584755264035E-5	3.34307542657921E-5	0.0	3.318280950498748	0.0
72-73	1.1143584755264035E-5	3.34307542657921E-5	0.0	3.6805588908923816	0.0
74-75	1.1143584755264035E-5	3.900254664342412E-5	0.0	4.052275447573724	0.0
76-77	1.1143584755264035E-5	4.457433902105614E-5	0.0	4.452034835291688	1.1143584755264035E-5
78-79	1.1143584755264035E-5	4.457433902105614E-5	0.0	4.896602577310569	1.1143584755264035E-5
80-81	1.1143584755264035E-5	4.457433902105614E-5	0.0	5.378785489670844	1.1143584755264035E-5
82-83	1.1143584755264035E-5	5.571792377632017E-5	0.0	5.872463009706173	1.1143584755264035E-5
84-85	1.1143584755264035E-5	5.571792377632017E-5	0.0	6.364625002214788	1.1143584755264035E-5
86-87	1.1143584755264035E-5	5.571792377632017E-5	0.0	6.888546211275903	1.1143584755264035E-5
88	1.1143584755264035E-5	5.571792377632017E-5	0.0	7.307216262323551	1.1143584755264035E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8995	0.0	42.714874	1
GTATCAA	15360	0.0	35.914104	1
TCAACGC	16555	0.0	32.3069	4
ATCAACG	16595	0.0	32.144962	3
CAACGCA	17945	0.0	29.883678	5
AACGCAG	18875	0.0	28.511024	6
TATCAAC	19105	0.0	28.241749	2
ACGCAGA	21255	0.0	24.876434	7
CGCAGAG	21540	0.0	24.131369	8
GTACATG	25465	0.0	21.681166	1
TACATGG	25660	0.0	20.532682	2
GCAGAGT	24775	0.0	20.145971	9
GTGGTAT	5495	0.0	19.427477	1
ACATGGG	26265	0.0	18.860687	3
AGAGTAC	21685	0.0	17.869493	10-11
TGGTATC	5740	0.0	16.703789	2
CAGAGTA	24065	0.0	16.629522	10-11
CATGGGG	17325	0.0	15.354136	4
GAGTACA	20205	0.0	14.686852	1
GAGTACT	9610	0.0	13.864203	12-13
>>END_MODULE