FastQCFastQC Report
Wed 25 May 2016
SRR2049465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8973773
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327370.36480753413307865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318910.35538006143012535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178030.1983892393979656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91020.10142890844241324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176950.059.977551
GTATCAA326700.041.1147421
GTGGTAT69350.034.950511
ATCAACG410400.032.322493
TCAACGC415150.031.9866314
TATCAAC417550.031.9603862
TGGTATC73000.031.4233192
CAACGCA430050.030.8893185
AACGCAG445350.029.8763926
ACGCAGA500850.026.4340767
CGCAGAG507950.025.935058
GCAGAGT556550.023.4169679
GAGTACT413000.021.57042312-13
CAGAGTA543850.021.21137610-11
GTACATG253900.020.594161
AGAGTAC521250.020.23757610-11
GTACTTT446950.019.8162814-15
TACATGG254950.019.70962
AGTACTT424650.019.10822712-13
ACATGGG256350.018.7584723
Produced by FastQC (version 0.11.5)