##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049465_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8973773
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.942080883926973	33.0	31.0	34.0	30.0	34.0
2	32.158253278749086	33.0	31.0	34.0	30.0	34.0
3	32.15796287693036	34.0	31.0	34.0	30.0	34.0
4	35.84339953774182	37.0	35.0	37.0	35.0	37.0
5	35.899440514040194	37.0	35.0	37.0	35.0	37.0
6	35.95925548818763	37.0	35.0	37.0	35.0	37.0
7	35.930324736317715	37.0	35.0	37.0	35.0	37.0
8	35.941847648698044	37.0	35.0	37.0	35.0	37.0
9	37.676166981268636	39.0	37.0	39.0	35.0	39.0
10-11	37.648805023260564	39.0	37.0	39.0	35.0	39.0
12-13	37.62562391538097	39.0	37.0	39.0	35.0	39.0
14-15	38.92148464196721	40.0	38.0	41.0	35.5	41.0
16-17	38.92979329876073	40.0	38.0	41.0	35.5	41.0
18-19	39.015119225770476	40.0	38.5	41.0	36.0	41.0
20-21	39.03978326619138	40.0	39.0	41.0	35.5	41.0
22-23	39.00697176093044	40.0	39.0	41.0	35.0	41.0
24-25	38.98496490829443	40.0	38.5	41.0	35.0	41.0
26-27	38.88181554180164	40.0	38.0	41.0	35.0	41.0
28-29	38.79820377671688	40.0	38.0	41.0	35.0	41.0
30-31	38.677206399136686	40.0	38.0	41.0	35.0	41.0
32-33	38.525149399254914	40.0	38.0	41.0	34.5	41.0
34-35	38.40039797084236	40.0	38.0	41.0	34.0	41.0
36-37	38.25894219744582	40.0	38.0	41.0	34.0	41.0
38-39	38.14654683152783	40.0	38.0	41.0	34.0	41.0
40-41	38.04607805434793	40.0	38.0	41.0	33.5	41.0
42-43	37.8900760025911	40.0	38.0	41.0	33.0	41.0
44-45	37.6949759036695	40.0	37.0	41.0	33.0	41.0
46-47	37.418668045202395	40.0	37.0	41.0	33.0	41.0
48-49	37.14550596499376	40.0	37.0	41.0	32.0	41.0
50-51	36.9742429967863	40.0	37.0	41.0	31.0	41.0
52-53	36.8550807447436	40.0	36.5	41.0	31.0	41.0
54-55	36.71167144522153	40.0	36.0	41.0	31.0	41.0
56-57	36.56148294591361	39.5	36.0	41.0	31.0	41.0
58-59	36.38450471167479	39.0	35.0	41.0	31.0	41.0
60-61	36.19559270108571	39.0	35.0	41.0	30.5	41.0
62-63	35.97624093009708	39.0	35.0	41.0	30.0	41.0
64-65	35.754236818782914	38.5	35.0	40.5	30.0	41.0
66-67	35.491115888489716	38.0	35.0	40.0	29.5	41.0
68-69	35.197690592351734	37.0	35.0	40.0	29.0	41.0
70-71	34.91151191366218	37.0	35.0	39.5	29.0	41.0
72-73	34.57875851105215	36.0	34.0	39.0	29.0	41.0
74-75	34.25348802560528	36.0	34.0	39.0	29.0	41.0
76-77	33.9333871048443	35.5	34.0	38.5	29.0	40.0
78-79	33.6251213397085	35.0	34.0	37.0	28.0	39.0
80-81	33.31492099254126	35.0	34.0	37.0	28.0	39.0
82-83	33.038499469509645	35.0	34.0	36.5	28.0	39.0
84-85	32.75638179169454	35.0	34.0	36.0	27.0	37.5
86-87	32.511129265248854	35.0	34.0	36.0	27.0	37.0
88-89	32.291760054550075	35.0	33.5	35.0	27.0	37.0
90-91	32.100163164367984	35.0	33.0	35.0	26.0	36.0
92-93	31.937333772539155	35.0	33.0	35.0	26.0	36.0
94-95	31.778854000429916	35.0	33.0	35.0	26.0	36.0
96-97	31.634102456124083	35.0	33.0	35.0	25.5	36.0
98-99	31.4962957609915	35.0	33.0	35.0	25.0	35.0
100	31.38719878472522	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2758866424679667
1101	2	0.32730994949737635
1101	3	0.42822656843123497
1101	4	0.25832349020899414
1101	5	0.2584892528456777
1101	6	0.2134375562333588
1101	7	0.23816625364398192
1101	8	0.2557503794411531
1101	9	0.2968147710757165
1101	10-11	0.3070982250924956
1101	12-13	0.2854583995623088
1101	14-15	0.41013066629923856
1101	16-17	0.38868046813647084
1101	18-19	0.4100078407653953
1101	20-21	0.40684429709309455
1101	22-23	0.3741966546301043
1101	24-25	0.3618595141424237
1101	26-27	0.37580855841703453
1101	28-29	0.42017118063868963
1101	30-31	0.3806150330047444
1101	32-33	0.39857750425164085
1101	34-35	0.39027896703366594
1101	36-37	0.4156908539790578
1101	38-39	0.3857106270732018
1101	40-41	0.4211112333831011
1101	42-43	0.42042479033138846
1101	44-45	0.41556866902302403
1101	46-47	0.3970622521963705
1101	48-49	0.34819500770827005
1101	50-51	0.280892088313216
1101	52-53	0.34057003770206506
1101	54-55	0.33472603098967824
1101	56-57	0.3634490968340671
1101	58-59	0.31823701589014775
1101	60-61	0.2714614108211606
1101	62-63	0.27764291537651786
1101	64-65	0.2581885291224779
1101	66-67	0.35556086496946193
1101	68-69	0.32119171358752396
1101	70-71	0.3391208894994904
1101	72-73	0.35457352254434227
1101	74-75	0.34378776474142825
1101	76-77	0.34545340901186705
1101	78-79	0.3102716153176033
1101	80-81	0.2709159802778913
1101	82-83	0.26744076184822774
1101	84-85	0.28463980669724265
1101	86-87	0.25019432543654574
1101	88-89	0.2849655954126149
1101	90-91	0.30147910340675566
1101	92-93	0.24322512329326784
1101	94-95	0.2579681593042338
1101	96-97	0.23703424370268777
1101	98-99	0.306872284434764
1101	100	0.33512006520298954
1102	1	0.26868677922907125
1102	2	0.2801920378945013
1102	3	0.356327427048555
1102	4	0.2253463375166973
1102	5	0.23754668697404213
1102	6	0.2248402687186868
1102	7	0.24631761139816888
1102	8	0.21751743338472096
1102	9	0.24991261728703051
1102	10-11	0.25310709937801334
1102	12-13	0.24964430198476606
1102	14-15	0.3364816434612976
1102	16-17	0.36393601880499915
1102	18-19	0.35482425289261954
1102	20-21	0.35634624951538996
1102	22-23	0.34286213606225857
1102	24-25	0.3221915806330742
1102	26-27	0.3013606838273617
1102	28-29	0.36921420063496413
1102	30-31	0.3857476015772505
1102	32-33	0.39447937248269227
1102	34-35	0.40421149382279253
1102	36-37	0.38797395116672817
1102	38-39	0.3230032199039101
1102	40-41	0.35884793788615355
1102	42-43	0.29450442401147114
1102	44-45	0.3313661128563794
1102	46-47	0.3174887279879073
1102	48-49	0.31634577930289254
1102	50-51	0.25173569895395786
1102	52-53	0.2642230415995357
1102	54-55	0.26034052278436803
1102	56-57	0.2583268854030436
1102	58-59	0.26084973771003206
1102	60-61	0.2457533712240263
1102	62-63	0.23585790621581992
1102	64-65	0.20596440838666297
1102	66-67	0.2163824598542874
1102	68-69	0.27583992701432436
1102	70-71	0.24740245841446296
1102	72-73	0.26789866099792903
1102	74-75	0.2539607094892844
1102	76-77	0.25767606718731173
1102	78-79	0.25946753709467885
1102	80-81	0.21674444018407257
1102	82-83	0.23730855519021787
1102	84-85	0.19875788563235375
1102	86-87	0.20730997613824087
1102	88-89	0.2303985217979232
1102	90-91	0.2307105048688598
1102	92-93	0.2121823716814326
1102	94-95	0.17878575467899793
1102	96-97	0.180110602837118
1102	98-99	0.2072745914339187
1102	100	0.2573713706853731
1103	1	0.24336970894397325
1103	2	0.2919276019998165
1103	3	0.373283296630774
1103	4	0.2205078286757285
1103	5	0.1856346050898594
1103	6	0.22337162034407498
1103	7	0.20221768071050406
1103	8	0.1764815728674307
1103	9	0.21662879276289715
1103	10-11	0.21489498243334282
1103	12-13	0.19883237261321796
1103	14-15	0.29951860139310327
1103	16-17	0.29893556599484583
1103	18-19	0.2832557712038195
1103	20-21	0.2721466227490694
1103	22-23	0.2989821278334972
1103	24-25	0.28613837324236613
1103	26-27	0.26313032361630206
1103	28-29	0.3073407416616618
1103	30-31	0.2891655448111976
1103	32-33	0.27936296906833036
1103	34-35	0.27940177558746626
1103	36-37	0.23427132808566142
1103	38-39	0.24720908165967614
1103	40-41	0.2615105857326938
1103	42-43	0.335245026808316
1103	44-45	0.284418917298062
1103	46-47	0.2699933586805443
1103	48-49	0.30505338195347065
1103	50-51	0.3076253967495646
1103	52-53	0.31899389063819683
1103	54-55	0.3149843199043403
1103	56-57	0.2895315379090775
1103	58-59	0.2783416177452338
1103	60-61	0.2532689057663262
1103	62-63	0.29997202551416535
1103	64-65	0.30366881022813885
1103	66-67	0.27503556456328226
1103	68-69	0.28699876633993426
1103	70-71	0.23570188769247835
1103	72-73	0.2706172096754571
1103	74-75	0.26021026133474123
1103	76-77	0.24009322582993775
1103	78-79	0.23208192802878358
1103	80-81	0.20039863120123869
1103	82-83	0.24057561986678166
1103	84-85	0.24170791005457914
1103	86-87	0.2072432709449643
1103	88-89	0.18279708519354188
1103	90-91	0.20226378998722794
1103	92-93	0.23145528708794316
1103	94-95	0.20401516534767694
1103	96-97	0.19810671776291855
1103	98-99	0.2711081160749309
1103	100	0.2438436245964759
1104	1	0.23095213380820923
1104	2	0.23167188693710017
1104	3	0.32688556871044483
1104	4	0.20360013505730734
1104	5	0.16667556960173613
1104	6	0.13428889125378873
1104	7	0.12176423428398664
1104	8	0.14806604068722606
1104	9	0.1965194480074075
1104	10-11	0.20223915354554123
1104	12-13	0.19992207491896608
1104	14-15	0.29142082326293206
1104	16-17	0.273194233800524
1104	18-19	0.2237356942225901
1104	20-21	0.2855677944040309
1104	22-23	0.27824183090353927
1104	24-25	0.25073081675914466
1104	26-27	0.28778141030080207
1104	28-29	0.2784252044306115
1104	30-31	0.31005372031324896
1104	32-33	0.26928316635496685
1104	34-35	0.29952863629171844
1104	36-37	0.2628306314528075
1104	38-39	0.2742441671152065
1104	40-41	0.2712243292594323
1104	42-43	0.23016409607293298
1104	44-45	0.22414932988346692
1104	46-47	0.23123263206790767
1104	48-49	0.22258491209072417
1104	50-51	0.2001404703448202
1104	52-53	0.25842144313029536
1104	54-55	0.23152907378874232
1104	56-57	0.22065361454202304
1104	58-59	0.20789913641699798
1104	60-61	0.18926934884295576
1104	62-63	0.17260608430765245
1104	64-65	0.14955184435132907
1104	66-67	0.1812269441878982
1104	68-69	0.22436664147999608
1104	70-71	0.19654497471548638
1104	72-73	0.1875921303565491
1104	74-75	0.20296228308628628
1104	76-77	0.19239273254346756
1104	78-79	0.204214361689111
1104	80-81	0.15770961476846423
1104	82-83	0.1948139680283063
1104	84-85	0.18527148821538475
1104	86-87	0.18496317154687603
1104	88-89	0.20242641226661817
1104	90-91	0.19935430640021679
1104	92-93	0.19637630871057965
1104	94-95	0.14813777355821145
1104	96-97	0.15065731182168918
1104	98-99	0.21575369003229028
1104	100	0.18646001686884617
1105	1	0.17865223204033498
1105	2	0.1950285504246665
1105	3	0.3043272790136058
1105	4	0.1630262368451909
1105	5	0.141697295951694
1105	6	0.1385552713026854
1105	7	0.17752655714894416
1105	8	0.13806397145285842
1105	9	0.216597906630021
1105	10-11	0.16949930823532355
1105	12-13	0.19116298505706908
1105	14-15	0.2436170281025909
1105	16-17	0.2579270872124084
1105	18-19	0.22853919546245294
1105	20-21	0.22801880343223502
1105	22-23	0.20650459409937127
1105	24-25	0.23196921168086249
1105	26-27	0.2246069915604707
1105	28-29	0.269877015187852
1105	30-31	0.23837335538021875
1105	32-33	0.24328495016085583
1105	34-35	0.21253078378676804
1105	36-37	0.2081967805502174
1105	38-39	0.2493940216548154
1105	40-41	0.28627803985119726
1105	42-43	0.25518744139870364
1105	44-45	0.27526459650159296
1105	46-47	0.262008913253581
1105	48-49	0.28127214691852487
1105	50-51	0.26344191759311997
1105	52-53	0.27117080129802673
1105	54-55	0.29436598189194996
1105	56-57	0.24110447731256812
1105	58-59	0.2943334753705429
1105	60-61	0.2632375282093449
1105	62-63	0.2670302949484977
1105	64-65	0.24456986171933437
1105	66-67	0.2338399589328617
1105	68-69	0.22568389824095192
1105	70-71	0.22118209045081727
1105	72-73	0.26875081057478667
1105	74-75	0.2626683943778545
1105	76-77	0.24565601961543138
1105	78-79	0.241529863358096
1105	80-81	0.20327534081832255
1105	82-83	0.21900040366244156
1105	84-85	0.21212604181091166
1105	86-87	0.1966320680218061
1105	88-89	0.1783763524347748
1105	90-91	0.20447810825884005
1105	92-93	0.17775162011141177
1105	94-95	0.18547927048035717
1105	96-97	0.2207398759225967
1105	98-99	0.2332349754355505
1105	100	0.2065784367020207
1106	1	0.16091282341667323
1106	2	0.19254093484483548
1106	3	0.24261228175032556
1106	4	0.13395155134595882
1106	5	0.15341602055250547
1106	6	0.14809645872217203
1106	7	0.151484154165189
1106	8	0.09650885008230148
1106	9	0.1523740825827531
1106	10-11	0.14854502559128235
1106	12-13	0.1744319433718431
1106	14-15	0.23238035901637488
1106	16-17	0.23922559729783188
1106	18-19	0.22438068353040563
1106	20-21	0.24313527473420038
1106	22-23	0.23365050690417632
1106	24-25	0.2284011895633853
1106	26-27	0.21556344148464035
1106	28-29	0.2396902352170045
1106	30-31	0.2626174131940502
1106	32-33	0.2691558827308569
1106	34-35	0.23486546125397467
1106	36-37	0.20807134682620188
1106	38-39	0.21191000166991358
1106	40-41	0.23856759808208494
1106	42-43	0.25582507796186604
1106	44-45	0.32080607109536885
1106	46-47	0.3033934516717096
1106	48-49	0.3246087575518004
1106	50-51	0.30891491121969494
1106	52-53	0.30027317676192666
1106	54-55	0.3406810650099388
1106	56-57	0.3199829295912693
1106	58-59	0.282410289054738
1106	60-61	0.2647500610435074
1106	62-63	0.294696476825024
1106	64-65	0.3199113647423317
1106	66-67	0.2939885401083302
1106	68-69	0.3052471777651462
1106	70-71	0.3361649264803148
1106	72-73	0.3574042714343477
1106	74-75	0.3229099803278217
1106	76-77	0.2554035081086923
1106	78-79	0.2401645670929824
1106	80-81	0.23906880557193233
1106	82-83	0.2570619676565542
1106	84-85	0.20255405391876025
1106	86-87	0.2462491076680564
1106	88-89	0.21886836251216835
1106	90-91	0.23977989601129224
1106	92-93	0.2629172964864459
1106	94-95	0.23200043793237768
1106	96-97	0.2656556053490746
1106	98-99	0.2934654315076024
1106	100	0.23798926600058934
1107	1	0.11132031559652944
1107	2	0.1277378159857463
1107	3	0.1858029838971973
1107	4	0.10809954944259204
1107	5	0.10563779979899834
1107	6	0.14040498702431847
1107	7	0.0811562765871443
1107	8	0.12497177548822691
1107	9	0.06864450443382708
1107	10-11	0.08687197007076719
1107	12-13	0.09988870004202255
1107	14-15	0.1384732116137002
1107	16-17	0.12099321638881122
1107	18-19	0.17430934911534024
1107	20-21	0.18050644445888508
1107	22-23	0.1876618048699612
1107	24-25	0.17826135975725066
1107	26-27	0.18291585767853746
1107	28-29	0.1968615333771524
1107	30-31	0.21175196192061208
1107	32-33	0.1432219743099452
1107	34-35	0.13515808881703606
1107	36-37	0.1374568504276681
1107	38-39	0.11115059874140343
1107	40-41	0.16903089598056908
1107	42-43	0.17553577489142214
1107	44-45	0.2276997950436126
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2216	62-63	-0.733413313644725
2216	64-65	-0.706157842543135
2216	66-67	-0.6023325936355022
2216	68-69	-0.6548698954821504
2216	70-71	-0.630781106291117
2216	72-73	-0.6523531941997973
2216	74-75	-0.6738204533006495
2216	76-77	-0.5503507026244563
2216	78-79	-0.5410298998142764
2216	80-81	-0.5246262444811975
2216	82-83	-0.5617445092773323
2216	84-85	-0.6556777889968899
2216	86-87	-0.6181570026216825
2216	88-89	-0.5879914726567748
2216	90-91	-0.5514951022349734
2216	92-93	-0.5744126764354185
2216	94-95	-0.5881685982274227
2216	96-97	-0.615297549774489
2216	98-99	-0.6942656651715708
2216	100	-0.7230175728223962
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	662.0
3	68.0
4	91.0
5	193.0
6	751.0
7	1711.0
8	3326.0
9	5362.0
10	7742.0
11	10667.0
12	15775.0
13	22712.0
14	27002.0
15	34531.0
16	44599.0
17	62748.0
18	65241.0
19	34910.0
20	23485.0
21	21923.0
22	22787.0
23	24672.0
24	28274.0
25	34520.0
26	43800.0
27	55762.0
28	72289.0
29	94421.0
30	123686.0
31	160612.0
32	208732.0
33	274594.0
34	378115.0
35	563292.0
36	938520.0
37	1797708.0
38	2646985.0
39	1117823.0
40	3682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.393440565016725	20.69884599123137	15.253583660363299	22.65412978338861
2	15.579054651817023	21.072217895415896	39.79543498593067	23.55329246683641
3	17.582459462703145	26.0282714973958	31.428096075084582	24.961172964816473
4	12.663413705695476	18.183366127046003	38.467420559891586	30.68579960736694
5	12.541639904226967	37.5628297380552	35.59324791865957	14.302282439058262
6	29.417407308518133	38.62931074061808	17.809214165586106	14.144067785277677
7	25.555783503772606	33.363781321412965	23.037054759464052	18.043380415350377
8	24.476928489276474	35.7073997748773	20.824897175357567	18.99077456048866
9	24.60239411003599	16.82825050288212	22.203503476185546	36.36585191089634
10-11	23.314262573836	27.17881319262255	29.044644877912557	20.462279355628898
12-13	24.430443025469888	24.92999878646362	29.243920032298565	21.395638155767923
14-15	21.736514841639075	26.778964656226538	27.367033910931333	24.117486591203054
16-17	20.365324596465722	29.90182056087222	28.03191032356178	21.700944519100272
18-19	20.182268929691	27.998891881931936	31.3246167470472	20.494222441329864
20-21	21.306456046971547	27.22115324290017	31.197251145086913	20.27513956504137
22-23	21.014299113650413	27.15537266208985	31.208233147863222	20.622095076396516
24-25	20.83964570978116	27.20590881895497	31.201374271446358	20.753071199817512
26-27	20.639172620033957	27.164861424508956	31.290612098166516	20.905353857290574
28-29	20.665204034022256	27.143075716312413	31.44344636308496	20.74827388658037
30-31	21.001027104206894	27.490047943044694	30.977092912869537	20.53183203987888
32-33	20.672893107503388	27.31291509156739	31.24426592916937	20.76992587175985
34-35	20.833923479009332	27.062011709010246	31.234448430999983	20.869616380980442
36-37	21.18002093433832	27.35449737808166	30.671535818880198	20.79394586869982
38-39	20.993978786849187	27.4651866054557	30.758773372136783	20.782061235558334
40-41	20.956068311511782	27.249235076483437	30.79012584784572	21.004570764159066
42-43	20.929084121026907	27.304033654517447	30.59350843842384	21.173373786031807
44-45	21.122982495768504	27.48048674732468	30.313397720223143	21.08313303668368
46-47	21.235727714529887	27.633131571302282	29.880681180591484	21.250459533576347
48-49	21.299039991316917	27.609479312659236	29.635488885221413	21.455991810802434
50-51	21.333245224723203	27.663057668162544	29.385939448212028	21.617757658902224
52-53	21.288810180511586	27.757666702734735	29.327212756551784	21.62631036020189
54-55	21.38304590499448	28.07250640282521	29.1240707782557	21.42037691392461
56-57	21.227214015776866	27.783686973138277	29.20051576967681	21.78858324140805
58-59	21.11590631944891	27.447284436546365	29.505042082076287	21.931767161928434
60-61	21.284698197736894	27.746941002407794	29.236453830512538	21.731906969342774
62-63	21.114502227769748	27.721929226424603	29.335642878419144	21.827925667386506
64-65	21.100901482575946	27.49499012288365	29.454149330499003	21.9499590640414
66-67	21.053630396044117	27.569217540938467	29.294394899447536	22.082757163569884
68-69	21.378889347880765	27.686932798500695	29.095593347413622	21.838584506204914
70-71	21.360368710017514	27.502629050233384	29.131815569660606	22.005186670088488
72-73	21.163589718616684	27.60961860746868	29.212093954237535	22.01469771967711
74-75	21.274418240800163	27.601868244271387	29.017159226113698	22.10655428881475
76-77	21.306907362154135	27.60487144036293	28.846472938417318	22.241748259065613
78-79	21.339023173418806	27.648236700438044	28.943617138521333	22.069122987621817
80-81	21.189478254260923	27.523296260050696	28.97665058496117	22.31057490072721
82-83	21.078220326068696	27.572175315205033	29.059657238119406	22.28994712060686
84-85	21.113063164685897	27.44266451654135	28.923869594847325	22.520402723925436
86-87	21.176730366120353	27.561979665199406	28.82557915301548	22.43571081566477
88-89	20.990734506491506	27.430110970626913	28.911145715837545	22.66800880704403
90-91	20.884495489576807	27.344501543925876	28.89220511751293	22.878797848984387
92-93	20.95847008869983	27.471149460498616	28.749216186010994	22.821164264790557
94-95	20.78958839009527	27.25072674455048	28.924435895065738	23.035248970288514
96-97	20.830949518092293	27.267668556260453	28.870968640617985	23.03041328502927
98-99	20.737545467041606	27.296550502940796	28.799096200378653	23.16680782963894
100	20.8286692725603	27.165365781415396	28.769988413999435	23.235976532024864
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1327.0
1	1171.0
2	2285.5
3	3383.0
4	2072.0
5	1217.5
6	2783.0
7	6959.5
8	11919.0
9	13884.5
10	10629.0
11	6609.5
12	5948.0
13	6592.5
14	7458.0
15	8346.0
16	9378.5
17	10546.5
18	11969.5
19	13676.0
20	15812.5
21	19018.0
22	23853.0
23	31113.5
24	41322.0
25	54526.5
26	71569.0
27	91378.0
28	114530.0
29	141062.5
30	166157.0
31	188904.5
32	209126.0
33	228331.5
34	247334.5
35	262836.5
36	276616.5
37	292121.5
38	305512.0
39	311982.0
40	316843.5
41	321363.5
42	321343.5
43	319991.0
44	316303.0
45	310097.0
46	303141.5
47	294437.5
48	286194.0
49	278647.0
50	269923.0
51	256602.0
52	238459.0
53	222737.5
54	211831.5
55	200766.5
56	185236.5
57	166941.5
58	150412.5
59	136067.5
60	119945.0
61	101346.0
62	85133.5
63	70436.0
64	57224.5
65	46940.5
66	37623.0
67	29407.5
68	22843.5
69	17735.5
70	13254.0
71	9659.5
72	7372.0
73	5477.0
74	3944.0
75	2737.0
76	1770.0
77	1202.5
78	815.5
79	448.0
80	230.0
81	116.0
82	64.0
83	42.0
84	20.0
85	11.0
86	8.5
87	6.5
88	6.0
89	5.0
90	2.5
91	1.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1343470578094632
2	0.0
3	0.0
4	0.0
5	0.014598096029395886
6	2.7858961888160085E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.6715377132896052E-4
82-83	5.627510301408337E-4
84-85	3.621665045460811E-4
86-87	4.847459368539855E-4
88-89	5.237484834974096E-4
90-91	6.51899708182946E-4
92-93	2.6744603412633686E-4
94-95	2.3958707223817677E-4
96-97	7.020458395816341E-4
98-99	0.0011422174374145634
100	1.3372301706316843E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8973773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.13950579149137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31320096725929	40.596273645027324
2	16.2579836510721	18.25430334674668
3	5.2365470000230525	8.819314819056327
4	2.205292458228482	4.952161149226004
5	1.1476697821921824	3.2214807192048833
6	0.687456892889148	2.3156094131850593
7	0.4281169753860244	1.6823992793383642
8	0.3098220976847935	1.3914607557845975
9	0.2220008020055406	1.1216713778915197
>10	1.1043574602710602	10.846483723375528
>50	0.061560738851221555	2.34849363147034
>100	0.024167283139719723	2.389499966272827
>500	0.0012690633789280428	0.48699790895268763
>1k	4.7556659113325635E-4	0.555620031758103
>5k	1.9815256842375583E-5	0.10125235246440667
>10k+	5.944577052712676E-5	0.9169778802453864
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32737	0.36480753413307865	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31891	0.35538006143012535	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17803	0.1983892393979656	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9102	0.10142890844241324	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1143584755264035E-5	0.0
2	0.0	0.0	0.0	1.1143584755264035E-5	0.0
3	0.0	0.0	0.0	1.1143584755264035E-5	0.0
4	0.0	0.0	0.0	1.1143584755264035E-5	0.0
5	0.0	0.0	0.0	1.1143584755264035E-5	0.0
6	0.0	0.0	0.0	1.1143584755264035E-5	0.0
7	0.0	0.0	0.0	1.1143584755264035E-5	0.0
8	0.0	0.0	0.0	3.34307542657921E-5	0.0
9	0.0	0.0	0.0	5.571792377632017E-5	0.0
10-11	0.0	0.0	0.0	5.571792377632017E-5	0.0
12-13	0.0	0.0	0.0	7.243330090921622E-5	0.0
14-15	0.0	0.0	0.0	1.0029226279737632E-4	0.0
16-17	5.571792377632017E-6	0.0	0.0	1.337230170631684E-4	0.0
18-19	1.1143584755264035E-5	0.0	0.0	2.2287169510528068E-4	0.0
20-21	1.1143584755264035E-5	0.0	0.0	2.618742417487048E-4	0.0
22-23	1.1143584755264035E-5	0.0	0.0	5.961817844066259E-4	0.0
24-25	1.1143584755264035E-5	0.0	0.0	0.0011589328145474595	0.0
26-27	1.1143584755264035E-5	0.0	0.0	0.0023512963833607113	0.0
28-29	1.671537713289605E-5	0.0	0.0	0.005939530674555731	0.0
30-31	2.228716951052807E-5	0.0	0.0	0.015595446864992018	0.0
32-33	2.228716951052807E-5	0.0	0.0	0.03968787710587286	0.0
34-35	2.228716951052807E-5	0.0	0.0	0.07935346704223518	0.0
36-37	2.228716951052807E-5	0.0	0.0	0.1405596063105229	0.0
38-39	2.228716951052807E-5	0.0	0.0	0.21925003005981988	0.0
40-41	2.228716951052807E-5	0.0	0.0	0.30101608320156975	0.0
42-43	2.228716951052807E-5	0.0	0.0	0.38634808346500404	0.0
44-45	2.228716951052807E-5	0.0	0.0	0.4968422981058246	0.0
46-47	2.228716951052807E-5	2.228716951052807E-5	0.0	0.632108701657597	0.0
48-49	2.228716951052807E-5	2.228716951052807E-5	0.0	0.7748078762411307	0.0
50-51	2.228716951052807E-5	2.228716951052807E-5	0.0	0.9188164220334079	0.0
52-53	2.228716951052807E-5	2.228716951052807E-5	0.0	1.0634322931948468	0.0
54-55	2.228716951052807E-5	2.228716951052807E-5	0.0	1.2294939932177913	0.0
56-57	2.228716951052807E-5	2.228716951052807E-5	0.0	1.4262228384872229	0.0
58-59	2.228716951052807E-5	2.228716951052807E-5	0.0	1.6470664011670453	0.0
60-61	2.228716951052807E-5	2.228716951052807E-5	0.0	1.8766688214645053	0.0
62-63	3.34307542657921E-5	2.228716951052807E-5	0.0	2.107313166936583	0.0
64-65	3.34307542657921E-5	2.7858961888160085E-5	0.0	2.358277839209884	0.0
66-67	3.34307542657921E-5	3.34307542657921E-5	0.0	2.648233914541854	0.0
68-69	3.34307542657921E-5	3.34307542657921E-5	0.0	2.9762731907749393	0.0
70-71	3.34307542657921E-5	3.34307542657921E-5	0.0	3.3251621140851233	0.0
72-73	3.34307542657921E-5	3.34307542657921E-5	0.0	3.687941515792744	0.0
74-75	3.34307542657921E-5	3.34307542657921E-5	0.0	4.060198536334717	0.0
76-77	3.34307542657921E-5	3.34307542657921E-5	0.0	4.460520675082822	0.0
78-79	3.34307542657921E-5	3.34307542657921E-5	0.0	4.906392216518069	0.0
80-81	3.34307542657921E-5	3.34307542657921E-5	0.0	5.390831704791285	0.0
82-83	3.34307542657921E-5	3.34307542657921E-5	0.0	5.88611947282375	0.0
84-85	3.34307542657921E-5	3.34307542657921E-5	0.0	6.379869426159988	0.0
86-87	3.34307542657921E-5	3.34307542657921E-5	0.0	6.905841054816074	0.0
88	3.34307542657921E-5	3.34307542657921E-5	0.0	7.327062986772677	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17695	0.0	59.97755	1
GTATCAA	32670	0.0	41.114742	1
GTGGTAT	6935	0.0	34.95051	1
ATCAACG	41040	0.0	32.32249	3
TCAACGC	41515	0.0	31.986631	4
TATCAAC	41755	0.0	31.960386	2
TGGTATC	7300	0.0	31.423319	2
CAACGCA	43005	0.0	30.889318	5
AACGCAG	44535	0.0	29.876392	6
ACGCAGA	50085	0.0	26.434076	7
CGCAGAG	50795	0.0	25.93505	8
GCAGAGT	55655	0.0	23.416967	9
GAGTACT	41300	0.0	21.570423	12-13
CAGAGTA	54385	0.0	21.211376	10-11
GTACATG	25390	0.0	20.59416	1
AGAGTAC	52125	0.0	20.237576	10-11
GTACTTT	44695	0.0	19.81628	14-15
TACATGG	25495	0.0	19.7096	2
AGTACTT	42465	0.0	19.108227	12-13
ACATGGG	25635	0.0	18.758472	3
>>END_MODULE