FastQCFastQC Report
Wed 25 May 2016
SRR2049464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734491
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81580.1422619723354697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79150.047.178961
GTATCAA128850.037.8871271
ATCAACG149400.031.428573
TCAACGC149600.031.3854584
TATCAAC165950.028.7757192
CAACGCA174050.027.0032965
AACGCAG182100.025.8623226
ACGCAGA192100.024.3447577
GTGGTAT40200.023.8661771
CGCAGAG196700.023.4623458
GTACATG260950.023.4115731
TACATGG261150.022.7672332
ACATGGG270750.021.2482283
GCAGAGT213300.020.689169
TGGTATC44650.020.3163472
TAGACCG2551.3491444E-820.274185
CAGAGTA205850.018.54985610-11
CATGGGA100550.017.7621754
CATGGGG165650.017.7046664
AGAGTAC194000.017.68435510-11
Produced by FastQC (version 0.11.5)