FastQCFastQC Report
Wed 25 May 2016
SRR2049464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734491
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279750.4878375430356417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272820.47575277387304293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159600.2783158958659103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82120.14320364265982805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141050.061.0281561
GTATCAA249400.041.6481321
GTGGTAT50050.038.0838281
TGGTATC55350.033.119182
ATCAACG323500.031.6893943
TCAACGC326300.031.4174674
TATCAAC331200.031.0661932
CAACGCA348350.029.4557765
AACGCAG360000.028.512636
ACGCAGA395700.025.7739417
CGCAGAG401350.025.2940068
GCAGAGT426250.023.5517949
GAGTACT286200.022.35811212-13
GTACATG245100.021.8326681
CAGAGTA420650.021.73130810-11
TACATGG245150.021.2633762
GTACTTT309400.020.6132614-15
AGAGTAC407200.020.36000810-11
AGTACTT298550.019.52053612-13
ACATGGG255000.019.4835193
Produced by FastQC (version 0.11.5)