##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049464_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5734491 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77360675951885 31.0 31.0 34.0 30.0 34.0 2 32.046812175657784 33.0 31.0 34.0 30.0 34.0 3 32.029350294559706 34.0 31.0 34.0 30.0 34.0 4 35.7514623355412 37.0 35.0 37.0 33.0 37.0 5 35.83083171636332 37.0 35.0 37.0 35.0 37.0 6 35.91711295736622 37.0 35.0 37.0 35.0 37.0 7 35.89139960285926 37.0 35.0 37.0 35.0 37.0 8 35.89715669620896 37.0 35.0 37.0 35.0 37.0 9 37.60392212665431 39.0 37.0 39.0 35.0 39.0 10-11 37.575352023396675 39.0 37.0 39.0 35.0 39.0 12-13 37.55867260058478 39.0 37.0 39.0 35.0 39.0 14-15 38.81832310836306 40.0 38.0 41.0 35.0 41.0 16-17 38.81739460398491 40.0 38.0 41.0 35.0 41.0 18-19 38.90619585940583 40.0 38.0 41.0 35.0 41.0 20-21 38.93107321992484 40.0 38.0 41.0 35.0 41.0 22-23 38.893886048474045 40.0 38.0 41.0 35.0 41.0 24-25 38.856893576081994 40.0 38.0 41.0 35.0 41.0 26-27 38.73694570276595 40.0 38.0 41.0 35.0 41.0 28-29 38.647134767497235 40.0 38.0 41.0 35.0 41.0 30-31 38.5112260181418 40.0 38.0 41.0 34.0 41.0 32-33 38.352914757386486 40.0 38.0 41.0 34.0 41.0 34-35 38.22624065501193 40.0 38.0 41.0 34.0 41.0 36-37 38.08048464981461 40.0 38.0 41.0 34.0 41.0 38-39 37.96569869932658 40.0 37.0 41.0 33.0 41.0 40-41 37.85456791195592 40.0 37.0 41.0 33.0 41.0 42-43 37.67835462641759 40.0 37.0 41.0 33.0 41.0 44-45 37.44649376901978 40.0 37.0 41.0 33.0 41.0 46-47 37.12223159823601 40.0 37.0 41.0 32.0 41.0 48-49 36.799695387088406 40.0 36.5 41.0 31.0 41.0 50-51 36.59256566973424 40.0 36.0 41.0 31.0 41.0 52-53 36.44181514976656 39.5 36.0 41.0 30.5 41.0 54-55 36.27245922959858 39.0 35.0 41.0 30.0 41.0 56-57 36.088097880003644 39.0 35.0 41.0 30.0 41.0 58-59 35.87849819626537 39.0 35.0 41.0 30.0 41.0 60-61 35.66827718449641 38.5 35.0 41.0 29.0 41.0 62-63 35.432935111416164 38.0 35.0 40.0 29.0 41.0 64-65 35.19773847408602 37.5 35.0 40.0 29.0 41.0 66-67 34.91903213380229 37.0 35.0 40.0 29.0 41.0 68-69 34.62306279668064 36.5 34.0 39.5 28.5 41.0 70-71 34.33879135916335 36.0 34.0 39.0 28.0 41.0 72-73 34.00795162116394 35.5 34.0 39.0 28.0 41.0 74-75 33.69302428062055 35.0 34.0 38.5 27.5 40.0 76-77 33.396311372709455 35.0 34.0 37.0 27.0 39.5 78-79 33.11443360884166 35.0 34.0 37.0 27.0 39.0 80-81 32.834249456490554 35.0 34.0 36.5 27.0 39.0 82-83 32.595214553479984 35.0 33.5 36.0 26.5 38.0 84-85 32.34510098629504 35.0 33.0 36.0 26.0 37.0 86-87 32.12849536253523 35.0 33.0 35.0 26.0 37.0 88-89 31.936627156621224 35.0 33.0 35.0 26.0 36.0 90-91 31.764166340133762 35.0 33.0 35.0 25.5 36.0 92-93 31.617270565077177 35.0 33.0 35.0 25.0 36.0 94-95 31.47127783442332 35.0 33.0 35.0 25.0 36.0 96-97 31.33877522869946 35.0 33.0 35.0 25.0 35.0 98-99 31.207636475495384 35.0 33.0 35.0 24.0 35.0 100 31.098943393581052 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3646381089166937 1101 2 0.3763626729782388 1101 3 0.5241335675472243 1101 4 0.327215312357815 1101 5 0.27373138200602654 1101 6 0.27097943313904693 1101 7 0.2888616865557978 1101 8 0.29324042178342324 1101 9 0.3640751298602183 1101 10-11 0.3727869445015344 1101 12-13 0.33837198523715983 1101 14-15 0.4996006004958744 1101 16-17 0.47393495679747843 1101 18-19 0.45911517289204795 1101 20-21 0.4418016672945626 1101 22-23 0.4219494584964991 1101 24-25 0.40226386161702976 1101 26-27 0.44364760274965676 1101 28-29 0.5071826766310252 1101 30-31 0.44614780207443516 1101 32-33 0.4677862990718893 1101 34-35 0.43997226752714624 1101 36-37 0.470342839022976 1101 38-39 0.4585427576744081 1101 40-41 0.5103786217265025 1101 42-43 0.4589649038448087 1101 44-45 0.5194008736704347 1101 46-47 0.47020873617344705 1101 48-49 0.47552143936313485 1101 50-51 0.45655882045224416 1101 52-53 0.4514509194996137 1101 54-55 0.47155753006901335 1101 56-57 0.4978855971044851 1101 58-59 0.49697185765042917 1101 60-61 0.43145476620684065 1101 62-63 0.4805536093284033 1101 64-65 0.47460878325540534 1101 66-67 0.4578185029593129 1101 68-69 0.4700003067748071 1101 70-71 0.48146524356171483 1101 72-73 0.49563446076660256 1101 74-75 0.4394920391301085 1101 76-77 0.4599541730606802 1101 78-79 0.4759870959208783 1101 80-81 0.4201236137964841 1101 82-83 0.44265495628155094 1101 84-85 0.4339251957089587 1101 86-87 0.39414967979409 1101 88-89 0.3994986569213381 1101 90-91 0.4079286895424268 1101 92-93 0.35578938120812964 1101 94-95 0.37452575338773997 1101 96-97 0.4065931922184767 1101 98-99 0.5081508871314853 1101 100 0.5112546023434348 1102 1 0.2977306318829136 1102 2 0.31749966472316515 1102 3 0.3559026674010397 1102 4 0.2084538157643152 1102 5 0.22046978831519226 1102 6 0.16060662884230936 1102 7 0.20883427004601174 1102 8 0.17012089587012724 1102 9 0.26603694543642575 1102 10-11 0.25837173339575514 1102 12-13 0.27301182831708815 1102 14-15 0.32190169931398316 1102 16-17 0.3465837741879696 1102 18-19 0.3965629156488504 1102 20-21 0.3859778953510187 1102 22-23 0.3414316105689039 1102 24-25 0.30097196142229166 1102 26-27 0.2639203900543592 1102 28-29 0.29016851615430994 1102 30-31 0.3061803723845884 1102 32-33 0.32619822055611536 1102 34-35 0.3267948787979904 1102 36-37 0.30364903230420026 1102 38-39 0.2612830713167682 1102 40-41 0.24948298652117273 1102 42-43 0.19441525114757496 1102 44-45 0.2548525695472179 1102 46-47 0.24704198769328656 1102 48-49 0.22187360283548685 1102 50-51 0.1979441858169011 1102 52-53 0.2328961255886952 1102 54-55 0.2801259310917814 1102 56-57 0.24897260395344034 1102 58-59 0.28468782054126507 1102 60-61 0.3016766560804811 1102 62-63 0.20082176652897488 1102 64-65 0.2172917015875555 1102 66-67 0.20750979189039498 1102 68-69 0.17951206903738637 1102 70-71 0.15248432048977634 1102 72-73 0.20400459893250655 1102 74-75 0.16215048758670747 1102 76-77 0.15526432710379368 1102 78-79 0.16859347143940084 1102 80-81 0.09044098271059653 1102 82-83 0.05882848314424649 1102 84-85 0.06498068638313015 1102 86-87 0.07935260871704486 1102 88-89 0.0643872287454883 1102 90-91 0.09065153465600062 1102 92-93 0.08132843995740657 1102 94-95 0.07039753231869739 1102 96-97 0.07424555411101252 1102 98-99 0.06371910429865224 1102 100 0.04690988794444095 1103 1 0.30942373281259705 1103 2 0.32758204658904333 1103 3 0.43200077844260676 1103 4 0.2608654996218789 1103 5 0.26143290330558955 1103 6 0.2043725624515247 1103 7 0.26188904854016926 1103 8 0.23114192694622915 1103 9 0.28718486698596735 1103 10-11 0.27760296795590733 1103 12-13 0.2664005612144109 1103 14-15 0.3938344542388492 1103 16-17 0.36096313197105445 1103 18-19 0.33017985935474314 1103 20-21 0.3048091366704142 1103 22-23 0.34824889827268635 1103 24-25 0.36776547184466324 1103 26-27 0.32784269200409 1103 28-29 0.3420308040752289 1103 30-31 0.36576231072847065 1103 32-33 0.37580141986414617 1103 34-35 0.4388927035222139 1103 36-37 0.38793836941208326 1103 38-39 0.3548183486434482 1103 40-41 0.372279261488373 1103 42-43 0.3789463034533611 1103 44-45 0.36774153914090135 1103 46-47 0.4424145339082628 1103 48-49 0.45690586229886065 1103 50-51 0.4395903881743237 1103 52-53 0.4679950574925229 1103 54-55 0.44764142676058327 1103 56-57 0.42763586047096425 1103 58-59 0.4265639446803178 1103 60-61 0.3746151558757518 1103 62-63 0.4528921313468288 1103 64-65 0.426990856460705 1103 66-67 0.44220256319938045 1103 68-69 0.4098311865224389 1103 70-71 0.4013302953757929 1103 72-73 0.48298744461040144 1103 74-75 0.4501998267873759 1103 76-77 0.41488653419096977 1103 78-79 0.41163695058030925 1103 80-81 0.3494265047932217 1103 82-83 0.3961918521768766 1103 84-85 0.37050333421991155 1103 86-87 0.34542381034456326 1103 88-89 0.3641748889832286 1103 90-91 0.38330926895350714 1103 92-93 0.4180722827851291 1103 94-95 0.4468869207281685 1103 96-97 0.4416869265254775 1103 98-99 0.5034888960770019 1103 100 0.45318866228540244 1104 1 0.3343801265520767 1104 2 0.3017538488316447 1104 3 0.3937962164347084 1104 4 0.23929378855122252 1104 5 0.2490623205199185 1104 6 0.21790275232650913 1104 7 0.21683124615554306 1104 8 0.2262488513424259 1104 9 0.297081450011774 1104 10-11 0.3213846287756965 1104 12-13 0.2710121626988524 1104 14-15 0.3591285325355358 1104 16-17 0.39628331241348747 1104 18-19 0.34729857963275634 1104 20-21 0.3547288589872437 1104 22-23 0.31908500700113507 1104 24-25 0.3307089320969112 1104 26-27 0.3240184063128382 1104 28-29 0.30664344330341464 1104 30-31 0.3025502342762465 1104 32-33 0.34866829001501287 1104 34-35 0.3750358331447572 1104 36-37 0.3662667163779929 1104 38-39 0.3799070517896439 1104 40-41 0.3113604775208074 1104 42-43 0.3106542319673551 1104 44-45 0.2814871134003738 1104 46-47 0.27406559967167254 1104 48-49 0.23853402995760575 1104 50-51 0.2383422253953782 1104 52-53 0.2744517331806051 1104 54-55 0.2480574425744706 1104 56-57 0.2653032265780766 1104 58-59 0.31005602594225223 1104 60-61 0.25790384199289207 1104 62-63 0.2864598767997961 1104 64-65 0.23234381985270147 1104 66-67 0.29202056823428535 1104 68-69 0.287110967022997 1104 70-71 0.232485761125929 1104 72-73 0.2517361510806424 1104 74-75 0.2875222102599153 1104 76-77 0.2925220207754009 1104 78-79 0.24548019889223838 1104 80-81 0.2846714999134292 1104 82-83 0.2178432759239186 1104 84-85 0.2379318271356894 1104 86-87 0.21033197148368288 1104 88-89 0.21547454767824092 1104 90-91 0.2276681988172129 1104 92-93 0.27062489878701257 1104 94-95 0.2704788873068651 1104 96-97 0.30608896129742647 1104 98-99 0.3428318796095233 1104 100 0.2680285057817322 1105 1 0.25968468129910605 1105 2 0.2500665127340369 1105 3 0.37281452920598923 1105 4 0.19539869536540522 1105 5 0.22140893070191936 1105 6 0.19130038204234268 1105 7 0.2068487348565995 1105 8 0.12818407407517185 1105 9 0.22355485447891255 1105 10-11 0.21047025086986793 1105 12-13 0.24056542004143466 1105 14-15 0.28720349490593833 1105 16-17 0.2821664618942634 1105 18-19 0.2943910851798748 1105 20-21 0.282145394330648 1105 22-23 0.28252619571377124 1105 24-25 0.24814669644929666 1105 26-27 0.1815008244886016 1105 28-29 0.26015150676408894 1105 30-31 0.2673952339585668 1105 32-33 0.24733213457390946 1105 34-35 0.23867902085692805 1105 36-37 0.27528938915980206 1105 38-39 0.2455145653257773 1105 40-41 0.30293323519292414 1105 42-43 0.30708207011580413 1105 44-45 0.2796854245541738 1105 46-47 0.260084419558801 1105 48-49 0.31948756965637415 1105 50-51 0.3150823396846434 1105 52-53 0.3011881253391522 1105 54-55 0.32987578341134594 1105 56-57 0.33886847662024877 1105 58-59 0.3602723933171674 1105 60-61 0.31926823557027006 1105 62-63 0.30862654950688295 1105 64-65 0.320987264842131 1105 66-67 0.2769425480677725 1105 68-69 0.3459414522971258 1105 70-71 0.3572734165046114 1105 72-73 0.40006236118980354 1105 74-75 0.3758352147750159 1105 76-77 0.3462017594756759 1105 78-79 0.29708991136589447 1105 80-81 0.31262576429511313 1105 82-83 0.3272367327704657 1105 84-85 0.3164939188199014 1105 86-87 0.28857487857348474 1105 88-89 0.31023986999295516 1105 90-91 0.32673640631736234 1105 92-93 0.3028743230452591 1105 94-95 0.2666116396298541 1105 96-97 0.33378998935496185 1105 98-99 0.4006915697112383 1105 100 0.3903047925818903 1106 1 0.20183289821401473 1106 2 0.20790479647832427 1106 3 0.25813398132962817 1106 4 0.1719089840731982 1106 5 0.1762887050309203 1106 6 0.14843108360949486 1106 7 0.09483961077204128 1106 8 0.08854826070220412 1106 9 0.15434266399213925 1106 10-11 0.17298946389450265 1106 12-13 0.17399307754881477 1106 14-15 0.23334301602453422 1106 16-17 0.21407873591977733 1106 18-19 0.19605116346371432 1106 20-21 0.1994867620560683 1106 22-23 0.22539091300732395 1106 24-25 0.17588886638907297 1106 26-27 0.16206508620555127 1106 28-29 0.2261390418810194 1106 30-31 0.23365134775449548 1106 32-33 0.20609961445002511 1106 34-35 0.14999331378087533 1106 36-37 0.15369929367952295 1106 38-39 0.16637984973648656 1106 40-41 0.16107330819396992 1106 42-43 0.128103797214969 1106 44-45 0.14139812907803417 1106 46-47 -0.014627708943123707 1106 48-49 0.03810115302407269 1106 50-51 0.07863083788364378 1106 52-53 0.021244988864815184 1106 54-55 0.029985821948166347 1106 56-57 0.09899941271598323 1106 58-59 -0.0011322041082379997 1106 60-61 -0.009218053010066285 1106 62-63 0.014265329838096363 1106 64-65 0.024703495502166106 1106 66-67 0.06097341931410227 1106 68-69 0.06068900201790228 1106 70-71 0.050444832759531266 1106 72-73 0.14036896681180622 1106 74-75 0.09539666885438436 1106 76-77 0.042094957186542103 1106 78-79 -0.014743285436260578 1106 80-81 -0.020269502062994604 1106 82-83 0.01429621720132701 1106 84-85 -0.005922829567886367 1106 86-87 -0.051017635254822835 1106 88-89 -0.06266770536075228 1106 90-91 -0.03922600226973216 1106 92-93 -0.009491196510921895 1106 94-95 -0.02119523166067694 1106 96-97 0.043059644440770484 1106 98-99 0.020106140341273004 1106 100 0.025125241156963796 1107 1 0.17890965177579332 1107 2 0.1659997561810016 1107 3 0.2462581575990086 1107 4 0.13109773349718523 1107 5 0.1562224622535595 1107 6 0.11091072615344899 1107 7 0.09397586121984602 1107 8 0.1558496624087482 1107 9 0.08360824243693088 1107 10-11 0.12097749154914084 1107 12-13 0.06985017498401191 1107 14-15 0.13811316267148044 1107 16-17 0.13599758961642294 1107 18-19 0.15053892481389397 1107 20-21 0.15425502222710463 1107 22-23 0.15566529818102026 1107 24-25 0.18856195513255614 1107 26-27 0.17488909302176836 1107 28-29 0.21783268698471403 1107 30-31 0.22669318209773337 1107 32-33 0.21357701886430647 1107 34-35 0.1259841532003847 1107 36-37 0.1947130198980247 1107 38-39 0.2160265842283664 1107 40-41 0.29745873976470705 1107 42-43 0.23715474232108846 1107 44-45 0.20296207987874482 1107 46-47 0.21123263396324887 1107 48-49 0.198686132719736 1107 50-51 0.14383193255250148 1107 52-53 0.19322217679224707 1107 54-55 0.23616188766053625 1107 56-57 0.19944848555886097 1107 58-59 0.1504795606148761 1107 60-61 0.16251773926219215 1107 62-63 0.18264823169958788 1107 64-65 0.1350397167920221 1107 66-67 0.16971584319022526 1107 68-69 0.1336451610503886 1107 70-71 0.1100011519409918 1107 72-73 0.16557475752360773 1107 74-75 0.13418865759559395 1107 76-77 0.1725703161481107 1107 78-79 0.14880749080278832 1107 80-81 0.17641676563781772 1107 82-83 0.173835210108912 1107 84-85 0.19505388875525398 1107 86-87 0.15845381082603183 1107 88-89 0.18492264218305365 1107 90-91 0.20524369286843225 1107 92-93 0.2430288056283061 1107 94-95 0.2320658075201436 1107 96-97 0.2558866366509065 1107 98-99 0.30697007744214133 1107 100 0.2717431378425559 1108 1 0.0759626083152547 1108 2 0.1171212553403933 1108 3 0.23416440063309096 1108 4 0.1258870348351664 1108 5 0.14058628113771476 1108 6 0.15855320546033624 1108 7 0.1398278204326786 1108 8 0.1282149155588499 1108 9 0.20765621450142646 1108 10-11 0.08453374039490313 1108 12-13 0.11442637899508412 1108 14-15 0.19920192375489876 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-0.5534369008051172 2215 20-21 -0.5629458314819047 2215 22-23 -0.5328431021713556 2215 24-25 -0.5483516943783115 2215 26-27 -0.5008275976692644 2215 28-29 -0.4698087531986843 2215 30-31 -0.515139919628993 2215 32-33 -0.5129307530626122 2215 34-35 -0.5283526544860493 2215 36-37 -0.5206289766169547 2215 38-39 -0.5298245999490234 2215 40-41 -0.5631229985683674 2215 42-43 -0.4663206777034574 2215 44-45 -0.5061839261347671 2215 46-47 -0.458541554100762 2215 48-49 -0.4801330218508042 2215 50-51 -0.43687816558320236 2215 52-53 -0.5343774300977415 2215 54-55 -0.45547211644746 2215 56-57 -0.4913206021716121 2215 58-59 -0.48196527306598824 2215 60-61 -0.4800568985005995 2215 62-63 -0.43961900551051514 2215 64-65 -0.5057715792733575 2215 66-67 -0.394964036484275 2215 68-69 -0.3990271777957304 2215 70-71 -0.3530325380096997 2215 72-73 -0.33314910833033196 2215 74-75 -0.3271749083322888 2215 76-77 -0.37612474725722933 2215 78-79 -0.3922631797183058 2215 80-81 -0.43117714588665024 2215 82-83 -0.40679101174929855 2215 84-85 -0.37143632827323003 2215 86-87 -0.39186306460527476 2215 88-89 -0.39835583209267966 2215 90-91 -0.3760322468287747 2215 92-93 -0.3248750107284657 2215 94-95 -0.316758326511561 2215 96-97 -0.30220953258186256 2215 98-99 -0.3115550930851221 2215 100 -0.25553535823361884 2216 1 -0.6694593006895211 2216 2 -0.6561168426330077 2216 3 -0.6277780730031601 2216 4 -0.5410832164076069 2216 5 -0.5525417529850571 2216 6 -0.53756248082626 2216 7 -0.5822647661089135 2216 8 -0.4530443513080229 2216 9 -0.7539913809839192 2216 10-11 -0.7092375742447317 2216 12-13 -0.7308700374413064 2216 14-15 -0.9913469446779857 2216 16-17 -1.0327952404823364 2216 18-19 -0.9001021732286674 2216 20-21 -0.8988858599108553 2216 22-23 -0.9479759230873128 2216 24-25 -0.9089576839574889 2216 26-27 -0.9138622114888832 2216 28-29 -1.0698251705464017 2216 30-31 -1.0990696282861734 2216 32-33 -1.0355625275641245 2216 34-35 -0.9898237321900822 2216 36-37 -1.0750032919647836 2216 38-39 -1.0076291365118237 2216 40-41 -1.08406714608914 2216 42-43 -1.171040526635018 2216 44-45 -1.1856829231064552 2216 46-47 -1.1776827972872894 2216 48-49 -1.1907989613304863 2216 50-51 -1.0904600994904499 2216 52-53 -1.1745304387422877 2216 54-55 -1.1767296386529509 2216 56-57 -1.1244914342484336 2216 58-59 -1.2080769621207068 2216 60-61 -1.200219332027288 2216 62-63 -1.2297817107073712 2216 64-65 -1.2467026559902905 2216 66-67 -1.2144096707684326 2216 68-69 -1.2154439544689168 2216 70-71 -1.2100991283083644 2216 72-73 -1.2213559528100006 2216 74-75 -1.1761975107265386 2216 76-77 -1.0682585805387532 2216 78-79 -1.0256400902820815 2216 80-81 -1.01204476464385 2216 82-83 -1.023600716735551 2216 84-85 -1.1733358320742155 2216 86-87 -1.1445217659899143 2216 88-89 -1.1317082517941515 2216 90-91 -1.1303140591624974 2216 92-93 -1.1711399695104028 2216 94-95 -1.2017629265451646 2216 96-97 -1.1739567808007827 2216 98-99 -1.2896097531666193 2216 100 -1.360161596971274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 357.0 3 39.0 4 56.0 5 195.0 6 710.0 7 1646.0 8 3868.0 9 6380.0 10 6728.0 11 7706.0 12 10197.0 13 14084.0 14 17421.0 15 23516.0 16 31607.0 17 46202.0 18 47080.0 19 24469.0 20 16274.0 21 15411.0 22 15506.0 23 16676.0 24 19306.0 25 23577.0 26 29667.0 27 38749.0 28 50181.0 29 65744.0 30 85504.0 31 111584.0 32 144987.0 33 189005.0 34 259430.0 35 392994.0 36 678521.0 37 1261267.0 38 1544593.0 39 531431.0 40 1823.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99245464599027 20.287863915447897 15.376015844964067 22.343665593597763 2 16.059524725036624 20.793545582336776 38.88345103340471 24.26347865922189 3 18.475536887232014 25.557717328355732 30.60761626446009 25.359129519952162 4 13.170532484923248 17.951166023279136 37.04790887281888 31.83039261897874 5 13.20530162608035 37.45911080074812 34.71525281367222 14.620334759499304 6 30.363455226786556 37.495825696064635 17.32632149089483 14.81439758625398 7 26.656541966845882 33.442061379118044 21.61093286221916 18.29046379181692 8 25.681564414348195 34.99705553640244 19.724680010832696 19.59670003841666 9 25.6472283241878 16.491507267166345 21.22922505240657 36.63203935623929 10-11 24.48630575930802 26.838746455439548 27.53447516091664 21.140472624335796 12-13 25.381485470986004 24.66708902324548 28.045871900400577 21.90555360536794 14-15 22.67249176953979 26.17877506477907 26.47441595077924 24.674317214901897 16-17 21.145922105379537 29.248541849660242 27.320689839778282 22.284846205181942 18-19 20.93365391976376 27.229112400734433 30.673969145648673 21.163264533853134 20-21 22.261443953787705 26.134411929498192 30.690814581451082 20.913329535263024 22-23 22.078794787540865 26.213372730029572 30.576881191373396 21.13095129105617 24-25 21.767250136062643 26.249871174268126 30.626161938348144 21.356716751321084 26-27 21.390172205344815 26.429154741022348 30.757289531015047 21.423383522617787 28-29 21.328196347330568 26.297268580594164 30.992698392934965 21.381836679140314 30-31 21.652880787501456 26.403520382192596 30.628864880945844 21.31473394936011 32-33 21.43947039065891 26.33068915793921 30.816021857912062 21.413818593489815 34-35 21.783005675656305 26.235763557742093 30.639519706282563 21.341711060319042 36-37 21.785490639012252 26.26924516927483 30.365511080233627 21.57975311147929 38-39 21.67825357124111 26.395263328515124 30.41269050731791 21.51379259292586 40-41 21.720323564898784 26.26316790801485 30.371544745645256 21.644963781441106 42-43 21.753927244806906 26.317994046899717 30.30384911232749 21.624229595965886 44-45 21.816216992929277 26.45221694479946 29.97183185046415 21.759734211807118 46-47 22.052576244343218 26.530515088435923 29.531583535487282 21.885325131733573 48-49 22.136297711514413 26.68231583239036 28.922218205591392 22.259168250503837 50-51 22.180538778419916 26.91409751972756 28.60198926112187 22.303374440730657 52-53 22.186214957875077 26.994714962496236 28.58801243214088 22.231057647487805 54-55 22.249559725527515 27.07114720382332 28.52007266207236 22.1592204085768 56-57 22.11561584105721 26.96945552796229 28.51601824817582 22.398910382804683 58-59 22.24014302228393 26.552530991852635 28.781264108706424 22.426061877157014 60-61 22.08908340775145 26.931466105710168 28.60419521104837 22.375255275490012 62-63 21.998491234880305 26.78238574269277 28.81667265673623 22.402450365690697 64-65 22.167154852976488 26.632363709351015 28.720857701232767 22.47962373643973 66-67 22.00820438989267 26.6956648811551 28.693383597602644 22.60274713134958 68-69 22.232182420375235 26.800050780444156 28.580452912037007 22.387313887143602 70-71 22.106722287993826 26.848572959657623 28.453030966479847 22.5916737858687 72-73 22.131589359892622 26.7807203812858 28.532750334772523 22.554939924049055 74-75 22.18966774906439 26.763691842920323 28.30680177194454 22.739838636070754 76-77 22.208509874721226 26.69916998736244 28.16219434296784 22.9301257949485 78-79 22.22976721037665 26.791549590015922 28.33323829438393 22.645444905223496 80-81 21.898531428715916 26.825669611133346 28.45545851109559 22.820340449055145 82-83 22.003777860280902 26.810932427325803 28.51572152440429 22.669568187989007 84-85 22.02393506987783 26.65886007823736 28.34456002709927 22.97264482478554 86-87 22.081854665345187 26.784757820188567 28.22075838414667 22.912629130319576 88-89 21.98819576122657 26.723510674185764 28.1646650415806 23.12362852300707 90-91 21.86189174286393 26.590778064814497 28.200136787120943 23.34719340520063 92-93 21.800797037849794 26.713027137755923 28.36130999080475 23.12486583358953 94-95 21.687690655876267 26.532706272808298 28.368417298793037 23.411185772522394 96-97 21.733181281990223 26.801874245843095 28.127542466243373 23.337402005923305 98-99 21.57401731818392 26.785873412114707 28.285184155021376 23.35492511468 100 21.625191298682637 26.668030345516463 28.138309819464265 23.568468536336635 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2609.0 1 1787.5 2 1582.0 3 2178.0 4 1432.0 5 1039.0 6 2687.5 7 7066.5 8 11872.0 9 13511.5 10 10007.0 11 5591.0 12 4513.0 13 4814.5 14 5477.5 15 6057.0 16 6735.0 17 7527.0 18 8373.0 19 9275.0 20 10534.5 21 12250.0 22 14447.0 23 17936.0 24 22860.5 25 28960.5 26 36789.0 27 45937.0 28 56911.0 29 69776.5 30 81947.5 31 92910.0 32 103097.0 33 112062.5 34 122210.0 35 133133.0 36 142303.5 37 153316.0 38 164883.0 39 173879.0 40 183312.5 41 189398.0 42 189759.0 43 191981.5 44 194842.5 45 196997.5 46 199591.5 47 196620.0 48 194675.5 49 200698.0 50 203385.5 51 196859.0 52 186352.0 53 177336.0 54 170599.0 55 162804.5 56 147958.5 57 129186.0 58 114647.5 59 106193.0 60 95408.5 61 79813.0 62 67970.0 63 57360.5 64 46095.5 65 36443.0 66 28680.5 67 22127.0 68 16770.0 69 12368.5 70 8655.5 71 6147.5 72 4579.0 73 3198.5 74 2340.0 75 1682.0 76 991.0 77 654.0 78 453.0 79 250.0 80 147.0 81 83.5 82 39.0 83 22.0 84 14.5 85 5.5 86 6.0 87 5.5 88 2.5 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12194630700440544 2 0.0 3 0.0 4 0.0 5 0.01225915255599843 6 3.487667867993864E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0463003603981591E-4 82-83 4.8827350151914095E-4 84-85 3.2260927778943237E-4 86-87 3.9236263514930966E-4 88-89 4.446776531692177E-4 90-91 5.754651982189875E-4 92-93 1.3950671471975456E-4 94-95 1.7438339339969318E-4 96-97 4.2723931382924833E-4 98-99 9.765470030382819E-4 100 5.2315018019907957E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5734491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.28683919107057 #Duplication Level Percentage of deduplicated Percentage of total 1 67.30506195895772 30.480335176803596 2 16.703831184752254 15.129274334773301 3 6.530433367787486 8.8722805732498 4 3.1745343885539437 5.75058513443864 5 1.7369120002977567 3.932962722326262 6 1.0760832078914058 2.9239444315193674 7 0.7026049016237004 2.2273128636283333 8 0.4873688699566381 1.765711651236804 9 0.34244140087162356 1.3957279788274335 >10 1.7508366416284054 14.198404540845011 >50 0.12167142892220807 3.784745749344716 >100 0.0614456709691804 5.151899986553904 >500 0.004465335262714764 1.370881981815969 >1k 0.0021556663566388882 1.6312681715731703 >5k 3.849404207773579E-5 0.14315761927708207 >10k+ 1.1548212623320738E-4 1.2415070837866482 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27975 0.4878375430356417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27282 0.47575277387304293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15960 0.2783158958659103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8212 0.14320364265982805 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.487667867993864E-5 0.0 9 0.0 0.0 0.0 5.2315018019907957E-5 0.0 10-11 0.0 0.0 0.0 7.847252702986193E-5 0.0 12-13 0.0 0.0 0.0 9.591086636983126E-5 0.0 14-15 0.0 0.0 0.0 1.0463003603981591E-4 1.743833933996932E-5 16-17 0.0 0.0 0.0 1.4822588438973921E-4 1.743833933996932E-5 18-19 0.0 0.0 0.0 1.7438339339969318E-4 1.743833933996932E-5 20-21 0.0 0.0 0.0 2.0054090240964714E-4 1.743833933996932E-5 22-23 0.0 0.0 0.0 4.1852014415926365E-4 1.743833933996932E-5 24-25 0.0 0.0 0.0 7.672869309586501E-4 1.743833933996932E-5 26-27 0.0 0.0 0.0 0.0016392038979571159 1.743833933996932E-5 28-29 0.0 0.0 0.0 0.003906188012153127 1.743833933996932E-5 30-31 0.0 0.0 0.0 0.010210147683552035 1.743833933996932E-5 32-33 0.0 0.0 0.0 0.023044765437769454 1.743833933996932E-5 34-35 0.0 0.0 0.0 0.045522784846989905 1.743833933996932E-5 36-37 0.0 0.0 0.0 0.08202994825521567 1.743833933996932E-5 38-39 0.0 0.0 0.0 0.12924425201818263 1.743833933996932E-5 40-41 0.0 0.0 0.0 0.1768160417376189 1.743833933996932E-5 42-43 0.0 0.0 0.0 0.225643391889533 1.743833933996932E-5 44-45 0.0 0.0 0.0 0.2896769739459003 1.743833933996932E-5 46-47 0.0 0.0 0.0 0.37065190267104786 1.743833933996932E-5 48-49 0.0 0.0 0.0 0.45677114150148634 1.743833933996932E-5 50-51 0.0 0.0 0.0 0.5441372215947327 1.743833933996932E-5 52-53 0.0 0.0 0.0 0.6315556167059988 1.743833933996932E-5 54-55 0.0 0.0 0.0 0.7317039995354426 1.743833933996932E-5 56-57 0.0 0.0 0.0 0.8563445299678734 1.743833933996932E-5 58-59 0.0 0.0 0.0 0.9938109589848514 1.743833933996932E-5 60-61 0.0 0.0 0.0 1.141879898320531 1.743833933996932E-5 62-63 0.0 0.0 0.0 1.291309028124728 1.743833933996932E-5 64-65 0.0 0.0 0.0 1.4502333336995386 1.743833933996932E-5 66-67 0.0 0.0 0.0 1.6377565157918985 1.743833933996932E-5 68-69 0.0 0.0 0.0 1.8493358870037464 1.743833933996932E-5 70-71 0.0 0.0 0.0 2.079164480334872 1.743833933996932E-5 72-73 0.0 0.0 0.0 2.318104605971132 1.743833933996932E-5 74-75 0.0 0.0 0.0 2.5648222309530175 1.743833933996932E-5 76-77 0.0 0.0 0.0 2.8308179400752396 1.743833933996932E-5 78-79 0.0 0.0 0.0 3.1378460616644093 1.743833933996932E-5 80-81 0.0 0.0 0.0 3.474972756954366 1.743833933996932E-5 82-83 0.0 0.0 0.0 3.824001118843852 1.743833933996932E-5 84-85 0.0 0.0 0.0 4.172959727375979 1.743833933996932E-5 86-87 0.0 0.0 0.0 4.544657930407424 1.743833933996932E-5 88 0.0 0.0 0.0 4.843097669871659 1.743833933996932E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14105 0.0 61.028156 1 GTATCAA 24940 0.0 41.648132 1 GTGGTAT 5005 0.0 38.083828 1 TGGTATC 5535 0.0 33.11918 2 ATCAACG 32350 0.0 31.689394 3 TCAACGC 32630 0.0 31.417467 4 TATCAAC 33120 0.0 31.066193 2 CAACGCA 34835 0.0 29.455776 5 AACGCAG 36000 0.0 28.51263 6 ACGCAGA 39570 0.0 25.773941 7 CGCAGAG 40135 0.0 25.294006 8 GCAGAGT 42625 0.0 23.551794 9 GAGTACT 28620 0.0 22.358112 12-13 GTACATG 24510 0.0 21.832668 1 CAGAGTA 42065 0.0 21.731308 10-11 TACATGG 24515 0.0 21.263376 2 GTACTTT 30940 0.0 20.61326 14-15 AGAGTAC 40720 0.0 20.360008 10-11 AGTACTT 29855 0.0 19.520536 12-13 ACATGGG 25500 0.0 19.483519 3 >>END_MODULE