FastQCFastQC Report
Wed 25 May 2016
SRR2049463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6128214
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82030.13385629157206325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61900.043.7679141
GTATCAA101000.031.8536151
TCAACGC104550.029.5791324
ATCAACG105800.029.2315713
CAACGCA112600.027.5490635
AACGCAG119450.026.0870596
TATCAAC122400.025.5743582
ACGCAGA137800.022.2730377
GTCTCGC17750.022.258961
CGCAGAG139350.021.686058
TCTGTCG17400.021.6081318
GTGGTAT37850.021.3740351
GTACATG148950.021.2519381
TACATGG151650.019.9597632
GCAGAGT159500.018.5636529
ACATGGG156700.018.2067153
CTGTCGC22450.017.7945149
TCGCTCT22250.017.7432354
TGGTATC41300.017.6397972
CAGAGTA152000.017.1917510-11
Produced by FastQC (version 0.11.5)