FastQCFastQC Report
Wed 25 May 2016
SRR2049463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6128214
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245110.39996971385137664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232950.380127064753287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128780.2101427920108534No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70440.11494376665044662No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67410.1099994223439325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129000.057.903861
GTATCAA224150.038.972611
GTGGTAT50050.036.1116561
TGGTATC53250.033.4569742
TATAACG6500.031.0972652
ATCAACG277550.031.0616973
TCAACGC280300.030.8072624
TATCAAC288650.029.948652
CAACGCA291600.029.6456725
ATAACGC6350.028.8707433
AACGCAG303700.028.552046
ACGCAGA340800.025.374877
CGCAGAG345500.024.9480658
TAACGCA8000.024.6789344
GCAGAGT376150.022.565369
GAGTACT301750.021.07347312-13
CAGAGTA371200.020.96074910-11
GTACATG146650.020.9259821
GTATAAC17550.020.3824671
AGAGTAC357450.020.20239410-11
Produced by FastQC (version 0.11.5)