##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049463_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6128214 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969994030887303 33.0 31.0 34.0 30.0 34.0 2 32.12909111855428 33.0 31.0 34.0 30.0 34.0 3 32.08269081334301 34.0 31.0 34.0 30.0 34.0 4 35.81405610182673 37.0 35.0 37.0 35.0 37.0 5 35.874051885263796 37.0 35.0 37.0 35.0 37.0 6 35.9389229880027 37.0 35.0 37.0 35.0 37.0 7 35.92848226253196 37.0 35.0 37.0 35.0 37.0 8 35.94139010158588 37.0 35.0 37.0 35.0 37.0 9 37.66707216817167 39.0 37.0 39.0 35.0 39.0 10-11 37.638853424505086 39.0 37.0 39.0 35.0 39.0 12-13 37.6180272098853 39.0 37.0 39.0 35.0 39.0 14-15 38.913039345558104 40.0 38.0 41.0 35.0 41.0 16-17 38.917635627607 40.0 38.0 41.0 35.5 41.0 18-19 39.002252858663226 40.0 38.5 41.0 36.0 41.0 20-21 39.02455919457121 40.0 39.0 41.0 35.5 41.0 22-23 38.98246773040236 40.0 39.0 41.0 35.0 41.0 24-25 38.954436888137394 40.0 38.0 41.0 35.0 41.0 26-27 38.846542810025895 40.0 38.0 41.0 35.0 41.0 28-29 38.7542943180509 40.0 38.0 41.0 35.0 41.0 30-31 38.62142746646902 40.0 38.0 41.0 35.0 41.0 32-33 38.45099918181708 40.0 38.0 41.0 34.0 41.0 34-35 38.310309251602504 40.0 38.0 41.0 34.0 41.0 36-37 38.15609156272937 40.0 38.0 41.0 34.0 41.0 38-39 38.027898095595226 40.0 38.0 41.0 33.5 41.0 40-41 37.917961905377325 40.0 38.0 41.0 33.0 41.0 42-43 37.7577354511445 40.0 37.5 41.0 33.0 41.0 44-45 37.56036089797125 40.0 37.0 41.0 33.0 41.0 46-47 37.28015895006277 40.0 37.0 41.0 32.5 41.0 48-49 37.004810210609484 40.0 37.0 41.0 31.5 41.0 50-51 36.8366399247807 40.0 37.0 41.0 31.0 41.0 52-53 36.7118948522359 40.0 36.0 41.0 31.0 41.0 54-55 36.56405805998289 40.0 36.0 41.0 31.0 41.0 56-57 36.41694056375969 39.5 36.0 41.0 31.0 41.0 58-59 36.24219984811235 39.0 35.0 41.0 30.0 41.0 60-61 36.05025958623507 39.0 35.0 41.0 30.0 41.0 62-63 35.82289179196418 39.0 35.0 41.0 30.0 41.0 64-65 35.59580482992271 38.5 35.0 40.5 29.0 41.0 66-67 35.33261362935433 38.0 35.0 40.0 29.0 41.0 68-69 35.03498768156595 37.0 35.0 40.0 29.0 41.0 70-71 34.74351107843166 37.0 34.5 39.5 29.0 41.0 72-73 34.41210089921795 36.0 34.0 39.0 28.0 41.0 74-75 34.08887597593687 36.0 34.0 39.0 28.0 41.0 76-77 33.775142888286865 35.0 34.0 38.5 28.0 40.0 78-79 33.46829190364436 35.0 34.0 37.0 28.0 39.0 80-81 33.161889173583035 35.0 34.0 37.0 27.0 39.0 82-83 32.889389061804955 35.0 34.0 36.5 27.0 39.0 84-85 32.60577706653194 35.0 34.0 36.0 27.0 37.5 86-87 32.356589701338756 35.0 33.5 36.0 26.0 37.0 88-89 32.13553361550363 35.0 33.0 35.0 26.0 37.0 90-91 31.94283114134069 35.0 33.0 35.0 26.0 36.0 92-93 31.774494983367095 35.0 33.0 35.0 25.5 36.0 94-95 31.6113839203396 35.0 33.0 35.0 25.0 36.0 96-97 31.46123356658237 35.0 33.0 35.0 25.0 36.0 98-99 31.3221419813342 35.0 33.0 35.0 25.0 35.0 100 31.213584251463804 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3502017533650914 1101 2 0.3516056910283467 1101 3 0.4708533360295917 1101 4 0.3098847350887439 1101 5 0.27322388175688417 1101 6 0.25644751222728246 1101 7 0.2842647205528763 1101 8 0.25682712287034803 1101 9 0.3475336406288676 1101 10-11 0.3100909690042428 1101 12-13 0.29450449099894627 1101 14-15 0.4093560799455247 1101 16-17 0.3826040317700219 1101 18-19 0.4038741760680651 1101 20-21 0.3820027157268342 1101 22-23 0.4274874758328835 1101 24-25 0.3789750975387136 1101 26-27 0.3998811587351554 1101 28-29 0.4291435963819197 1101 30-31 0.43706795907124985 1101 32-33 0.4335538578742657 1101 34-35 0.45044761497447183 1101 36-37 0.4468664565348206 1101 38-39 0.4199445999488063 1101 40-41 0.43902133805413257 1101 42-43 0.453219417034866 1101 44-45 0.45381887922486897 1101 46-47 0.40618436249528145 1101 48-49 0.4612701400397654 1101 50-51 0.4025335693128014 1101 52-53 0.39383526136437297 1101 54-55 0.3905642580958286 1101 56-57 0.43869065803444585 1101 58-59 0.3740357437069406 1101 60-61 0.31421141310737966 1101 62-63 0.3432288351036519 1101 64-65 0.31199434303469786 1101 66-67 0.35032784265118266 1101 68-69 0.3431754457330456 1101 70-71 0.36938816827002086 1101 72-73 0.37621237804667373 1101 74-75 0.3198474024620026 1101 76-77 0.2825325489713677 1101 78-79 0.25120128057961466 1101 80-81 0.31174885213255266 1101 82-83 0.28831197936493425 1101 84-85 0.29304578227134925 1101 86-87 0.23842446515896398 1101 88-89 0.24295487585709452 1101 90-91 0.26126357482021234 1101 92-93 0.25111662688307845 1101 94-95 0.2283440446173799 1101 96-97 0.193955994724746 1101 98-99 0.2698112517979574 1101 100 0.2777111433849804 1102 1 0.2705529576457053 1102 2 0.24106784557432803 1102 3 0.32526517480367545 1102 4 0.1734826543789083 1102 5 0.22027211371020883 1102 6 0.1564067943834715 1102 7 0.2244191748385731 1102 8 0.18355529214765198 1102 9 0.23586813953821917 1102 10-11 0.2276512523187435 1102 12-13 0.20889133846966956 1102 14-15 0.2749034296437429 1102 16-17 0.3519333844524013 1102 18-19 0.3047316260206898 1102 20-21 0.314161801008332 1102 22-23 0.24288053782799324 1102 24-25 0.2599726880255844 1102 26-27 0.2748999105719321 1102 28-29 0.3058616562352512 1102 30-31 0.28922795076682917 1102 32-33 0.32358458467919604 1102 34-35 0.2953415033758304 1102 36-37 0.37056597071615016 1102 38-39 0.3734994707451875 1102 40-41 0.4005712088426776 1102 42-43 0.38121252196189204 1102 44-45 0.47179419415631685 1102 46-47 0.43335831941419656 1102 48-49 0.47036701001027126 1102 50-51 0.44474936858326686 1102 52-53 0.46217600666869885 1102 54-55 0.4166284225736234 1102 56-57 0.44398819501005704 1102 58-59 0.45959753578368634 1102 60-61 0.3891127935076568 1102 62-63 0.41760417753176426 1102 64-65 0.42499737724757836 1102 66-67 0.4047565249425915 1102 68-69 0.4751811997304003 1102 70-71 0.4559095799456401 1102 72-73 0.4015394823762932 1102 74-75 0.37375740442966787 1102 76-77 0.3935255067755534 1102 78-79 0.3895694650174377 1102 80-81 0.3293622038982136 1102 82-83 0.30789625460779746 1102 84-85 0.2889767617154746 1102 86-87 0.28450394107630217 1102 88-89 0.2575601920163706 1102 90-91 0.27253458847491885 1102 92-93 0.28357255449381924 1102 94-95 0.26879767579836056 1102 96-97 0.297560789085928 1102 98-99 0.3375485117849877 1102 100 0.3530998983316991 1103 1 0.29253391416593644 1103 2 0.2640481388962286 1103 3 0.37444888407389243 1103 4 0.17296234362837026 1103 5 0.20134477409163765 1103 6 0.18374394506233926 1103 7 0.1881923395196523 1103 8 0.1517626403989425 1103 9 0.22787078761985669 1103 10-11 0.23156765335940577 1103 12-13 0.20852866086070776 1103 14-15 0.32380774894840414 1103 16-17 0.30157138773931536 1103 18-19 0.2933491226774052 1103 20-21 0.3038220595350083 1103 22-23 0.3063983550631235 1103 24-25 0.32841079677379525 1103 26-27 0.33597917248310694 1103 28-29 0.3608240167708203 1103 30-31 0.35176285027176846 1103 32-33 0.3512239655017879 1103 34-35 0.357951703644261 1103 36-37 0.35494104792743997 1103 38-39 0.315134088859125 1103 40-41 0.3379152061163353 1103 42-43 0.363731159547271 1103 44-45 0.39718765716516913 1103 46-47 0.3502707214987879 1103 48-49 0.34705945284748196 1103 50-51 0.36702796997371223 1103 52-53 0.3793588853034109 1103 54-55 0.34369391590825416 1103 56-57 0.31066317990680403 1103 58-59 0.31189147543113904 1103 60-61 0.26043823260505405 1103 62-63 0.3183726101876019 1103 64-65 0.24568148712589277 1103 66-67 0.25827372132710025 1103 68-69 0.2311269218713221 1103 70-71 0.27368031017974914 1103 72-73 0.2766064802322461 1103 74-75 0.2694056365538984 1103 76-77 0.2611779291962293 1103 78-79 0.242105754756615 1103 80-81 0.23360112261947563 1103 82-83 0.20222810240062472 1103 84-85 0.1998591628381945 1103 86-87 0.18491189395164298 1103 88-89 0.24266894171800857 1103 90-91 0.22817261319426052 1103 92-93 0.237900264048573 1103 94-95 0.2221145638203481 1103 96-97 0.2844050116585528 1103 98-99 0.34197730133828586 1103 100 0.23442895479618286 1104 1 0.23311494973710722 1104 2 0.26130924760858676 1104 3 0.32907398699570223 1104 4 0.19527772942807786 1104 5 0.16366975014563678 1104 6 0.2041861700334877 1104 7 0.18307305049707878 1104 8 0.19495154967597017 1104 9 0.2537834928730476 1104 10-11 0.24035122222961292 1104 12-13 0.20532407808316577 1104 14-15 0.2998517585618714 1104 16-17 0.3348593001729441 1104 18-19 0.23862039667239543 1104 20-21 0.26708895648639697 1104 22-23 0.2641087722435458 1104 24-25 0.32360060183512473 1104 26-27 0.3303322178438748 1104 28-29 0.2788464770172254 1104 30-31 0.30877578264671257 1104 32-33 0.33813537461155363 1104 34-35 0.35362138925969333 1104 36-37 0.3481986879960175 1104 38-39 0.36806091270121044 1104 40-41 0.36209311264940425 1104 42-43 0.36018883208380004 1104 44-45 0.3549242863086377 1104 46-47 0.3560264203630581 1104 48-49 0.3743744981232666 1104 50-51 0.3953380412570411 1104 52-53 0.37627549198386845 1104 54-55 0.3519832584878415 1104 56-57 0.35130308476774275 1104 58-59 0.365535436239135 1104 60-61 0.3167768826663391 1104 62-63 0.3345229872386497 1104 64-65 0.2643074710094311 1104 66-67 0.3669107416187103 1104 68-69 0.32109045931415636 1104 70-71 0.3198116144855305 1104 72-73 0.3397391852840741 1104 74-75 0.3279684479716778 1104 76-77 0.28864441531460017 1104 78-79 0.2829726778327739 1104 80-81 0.24158068311923842 1104 82-83 0.24528468486008848 1104 84-85 0.2672794243504768 1104 86-87 0.2604690762958555 1104 88-89 0.2497012476491136 1104 90-91 0.24010568429806511 1104 92-93 0.25830334137118527 1104 94-95 0.2884199858589085 1104 96-97 0.2572791856939176 1104 98-99 0.31050844328248317 1104 100 0.324886071404773 1105 1 0.24086711318332732 1105 2 0.23085376435633265 1105 3 0.3190507231489832 1105 4 0.15059257839901363 1105 5 0.15213608225416664 1105 6 0.14969456496181266 1105 7 0.1605014373362792 1105 8 0.1635516967326609 1105 9 0.19976971122291332 1105 10-11 0.19418229959272537 1105 12-13 0.15447637653262092 1105 14-15 0.2642824999261748 1105 16-17 0.22983935331691185 1105 18-19 0.23241718283880886 1105 20-21 0.24249594005161157 1105 22-23 0.19611475768377318 1105 24-25 0.23439300547733666 1105 26-27 0.23218473789854954 1105 28-29 0.23177018292699358 1105 30-31 0.22072139965634818 1105 32-33 0.20127954897446898 1105 34-35 0.24919424688044245 1105 36-37 0.1996411255634456 1105 38-39 0.2192796102612249 1105 40-41 0.28983623627811284 1105 42-43 0.274845928836875 1105 44-45 0.2660678224372006 1105 46-47 0.3145229407519068 1105 48-49 0.3762916992012819 1105 50-51 0.26248080524162987 1105 52-53 0.24253963469627848 1105 54-55 0.2772437761072766 1105 56-57 0.274031501482213 1105 58-59 0.2855498686333888 1105 60-61 0.26009467114497653 1105 62-63 0.23305213714896666 1105 64-65 0.249126949179157 1105 66-67 0.2969599510451104 1105 68-69 0.31411110860562275 1105 70-71 0.3263475895380594 1105 72-73 0.33236088565332267 1105 74-75 0.3329717531606917 1105 76-77 0.2697939607227724 1105 78-79 0.26406594238456904 1105 80-81 0.29036519692495943 1105 82-83 0.26838062658154627 1105 84-85 0.23825239565278622 1105 86-87 0.2221642166130735 1105 88-89 0.23123078718502654 1105 90-91 0.2174759872478731 1105 92-93 0.23510104692708111 1105 94-95 0.22718955468447533 1105 96-97 0.24271644854344032 1105 98-99 0.33965879943920285 1105 100 0.2683216471136767 1106 1 0.14859753345820081 1106 2 0.18447605089889407 1106 3 0.2365192983435449 1106 4 0.12385269010470523 1106 5 0.1447976900528687 1106 6 0.1386478516729568 1106 7 0.1481597842561868 1106 8 0.10272286553106369 1106 9 0.17693898645786277 1106 10-11 0.1495372975148186 1106 12-13 0.1575689686123951 1106 14-15 0.19188378348852098 1106 16-17 0.21712654977169876 1106 18-19 0.17383489903713922 1106 20-21 0.18252412545468388 1106 22-23 0.1923121108891479 1106 24-25 0.14157858156895742 1106 26-27 0.1105666679102768 1106 28-29 0.1569109065479779 1106 30-31 0.1720530931848785 1106 32-33 0.16366714643699964 1106 34-35 0.1915034493401393 1106 36-37 0.16538699739094653 1106 38-39 0.1655646668558859 1106 40-41 0.1910625727987849 1106 42-43 0.19074455866139317 1106 44-45 0.23722487450574903 1106 46-47 0.2812477773592903 1106 48-49 0.2259354672692666 1106 50-51 0.22764461551053472 1106 52-53 0.22244148770214878 1106 54-55 0.2626550324001258 1106 56-57 0.26861056841911335 1106 58-59 0.19033076137942828 1106 60-61 0.21694228036950136 1106 62-63 0.2146155640460634 1106 64-65 0.2773840708616291 1106 66-67 0.28005014307160536 1106 68-69 0.26156174745084115 1106 70-71 0.2747170907656056 1106 72-73 0.2710964747217517 1106 74-75 0.25970817490788534 1106 76-77 0.23753117057884054 1106 78-79 0.2273816434073126 1106 80-81 0.20443740868914517 1106 82-83 0.20317079486775924 1106 84-85 0.1900598298197167 1106 86-87 0.1983761550880203 1106 88-89 0.1438766992568219 1106 90-91 0.17016944382184107 1106 92-93 0.15134304051710146 1106 94-95 0.13614552064237628 1106 96-97 0.17384278983440282 1106 98-99 0.20965878529003135 1106 100 0.18632945735549455 1107 1 0.1505736489796874 1107 2 0.19144183866983866 1107 3 0.2049068938441323 1107 4 0.1175634944249424 1107 5 0.12222478919927227 1107 6 0.14012896619900062 1107 7 0.12512843393390938 1107 8 0.1269017004861439 1107 9 0.15552601331001625 1107 10-11 0.13429958816011833 1107 12-13 0.1770742631399358 1107 14-15 0.21403342543909787 1107 16-17 0.17992837205972734 1107 18-19 0.16277883988276898 1107 20-21 0.19760707408967448 1107 22-23 0.2294546836189113 1107 24-25 0.19070927738559362 1107 26-27 0.15291025070648345 1107 28-29 0.20256473559260257 1107 30-31 0.20313900016046915 1107 32-33 0.16789681912196386 1107 34-35 0.19758947296953266 1107 36-37 0.18224094526691204 1107 38-39 0.16181094171092525 1107 40-41 0.20653047900289323 1107 42-43 0.18341733850610353 1107 44-45 0.17813077687739565 1107 46-47 0.1304128471649335 1107 48-49 0.06627120470798076 1107 50-51 0.07848094555060214 1107 52-53 0.08020997033161592 1107 54-55 0.1344019465180324 1107 56-57 0.10005009197384851 1107 58-59 0.05451073176492116 1107 60-61 0.13575045173551104 1107 62-63 0.12627130154698563 1107 64-65 0.08767192580028649 1107 66-67 0.15960916834349348 1107 68-69 0.15444477634365228 1107 70-71 0.12362280026962225 1107 72-73 0.11000398069442952 1107 74-75 0.12469169561316562 1107 76-77 0.09276534921159652 1107 78-79 0.13511100812622345 1107 80-81 0.14073081945986843 1107 82-83 0.15640000560364342 1107 84-85 0.144063427423184 1107 86-87 0.08845424314765182 1107 88-89 0.07040735276700616 1107 90-91 0.11005229229009927 1107 92-93 0.13048572834528827 1107 94-95 0.12098810799275128 1107 96-97 0.08634251764218348 1107 98-99 0.13683842282332037 1107 100 0.16244225692247483 1108 1 0.055514662114909186 1108 2 0.12322956803769358 1108 3 0.18124894738497233 1108 4 0.09422110562604757 1108 5 0.06860860054550244 1108 6 0.08928235762748216 1108 7 0.10225531739453686 1108 8 0.03889363883239838 1108 9 0.09744466385230766 1108 10-11 0.046716115651278756 1108 12-13 0.0768929164557477 1108 14-15 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3 -0.25949204529389647 2215 4 -0.203378233834151 2215 5 -0.2124318077616394 2215 6 -0.1897428835673054 2215 7 -0.23366769561648937 2215 8 -0.27750622357903865 2215 9 -0.4148490575394419 2215 10-11 -0.41808361134120275 2215 12-13 -0.41786455831690716 2215 14-15 -0.5532619422624663 2215 16-17 -0.529763570643361 2215 18-19 -0.5095362639728904 2215 20-21 -0.5096950126597548 2215 22-23 -0.510760755985757 2215 24-25 -0.4660829539441096 2215 26-27 -0.4213474265071966 2215 28-29 -0.3992092168543806 2215 30-31 -0.5218926984540033 2215 32-33 -0.508604202982113 2215 34-35 -0.5079388548459107 2215 36-37 -0.4702424574388573 2215 38-39 -0.4535717533533443 2215 40-41 -0.4563115315324211 2215 42-43 -0.4155522254925117 2215 44-45 -0.45683209192679186 2215 46-47 -0.5267148922683162 2215 48-49 -0.49050655165171975 2215 50-51 -0.5137827462446367 2215 52-53 -0.5078170554177746 2215 54-55 -0.4907291289213944 2215 56-57 -0.47219733564596567 2215 58-59 -0.4902026885118289 2215 60-61 -0.46543426843047797 2215 62-63 -0.5000727449820701 2215 64-65 -0.5437618582366781 2215 66-67 -0.5187476829408979 2215 68-69 -0.4664283515562744 2215 70-71 -0.4503487097194423 2215 72-73 -0.4168967701597168 2215 74-75 -0.41638090094745905 2215 76-77 -0.4461121237265999 2215 78-79 -0.46592179939673883 2215 80-81 -0.5631154015234472 2215 82-83 -0.5644638376214459 2215 84-85 -0.48657175511249307 2215 86-87 -0.4559176217442591 2215 88-89 -0.4341874653655182 2215 90-91 -0.4078715533140169 2215 92-93 -0.4138161608629112 2215 94-95 -0.49919932731862104 2215 96-97 -0.4496762696663339 2215 98-99 -0.45975976275138564 2215 100 -0.4201342924768916 2216 1 -0.39233153110034635 2216 2 -0.4198793545877706 2216 3 -0.3121698877557719 2216 4 -0.3005402587582964 2216 5 -0.30746327923517924 2216 6 -0.3113728376330087 2216 7 -0.36720199575201207 2216 8 -0.3316749649004933 2216 9 -0.4806698717298019 2216 10-11 -0.4740675143905122 2216 12-13 -0.46152976289183556 2216 14-15 -0.6751078974721025 2216 16-17 -0.6829941642190178 2216 18-19 -0.6339273982432658 2216 20-21 -0.5944057902136493 2216 22-23 -0.5931163327924267 2216 24-25 -0.6175709178526887 2216 26-27 -0.6199746046837902 2216 28-29 -0.7230987537359894 2216 30-31 -0.7023577610263203 2216 32-33 -0.6421570810963146 2216 34-35 -0.6509315237360696 2216 36-37 -0.6653509769005126 2216 38-39 -0.6365580935168538 2216 40-41 -0.6864341972168404 2216 42-43 -0.7806206556529176 2216 44-45 -0.7901118006317986 2216 46-47 -0.7002118749337782 2216 48-49 -0.6467614183770252 2216 50-51 -0.6719014213599408 2216 52-53 -0.6569707589824176 2216 54-55 -0.6129418224297822 2216 56-57 -0.6199848225336027 2216 58-59 -0.6584006884622511 2216 60-61 -0.6622389324352369 2216 62-63 -0.6373534407627162 2216 64-65 -0.6538582744907586 2216 66-67 -0.6352379733154265 2216 68-69 -0.6897105805826556 2216 70-71 -0.6684724238703978 2216 72-73 -0.6240897705687587 2216 74-75 -0.6445997697856001 2216 76-77 -0.5788119174523771 2216 78-79 -0.5773828654135329 2216 80-81 -0.591376127061821 2216 82-83 -0.6144316013731981 2216 84-85 -0.7074778510238033 2216 86-87 -0.7084602314673312 2216 88-89 -0.7057700644689291 2216 90-91 -0.6372844028067135 2216 92-93 -0.6604980169556569 2216 94-95 -0.657941353080858 2216 96-97 -0.7037647648874241 2216 98-99 -0.812699629442335 2216 100 -0.8390613153722803 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 475.0 3 54.0 4 63.0 5 159.0 6 558.0 7 1395.0 8 2647.0 9 4198.0 10 6118.0 11 8936.0 12 13064.0 13 18784.0 14 21309.0 15 26061.0 16 32098.0 17 44267.0 18 45823.0 19 24544.0 20 17254.0 21 15751.0 22 16231.0 23 17435.0 24 20359.0 25 24688.0 26 30682.0 27 39283.0 28 50488.0 29 65718.0 30 86089.0 31 110681.0 32 143791.0 33 189400.0 34 261464.0 35 390277.0 36 635402.0 37 1221007.0 38 1779147.0 39 760072.0 40 2442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.03897496833296 20.629997607814992 14.581151935120806 22.749875488731238 2 16.021307349906515 21.087824282898737 39.5496306101582 23.341237757036552 3 17.57892266817053 25.986951500061846 31.33962032004757 25.094505511720055 4 12.739976117022023 18.350778872931002 38.4618911806931 30.44735382935387 5 12.232304005674246 37.06591432194072 35.43187549950362 15.269906172881415 6 29.023873241734933 38.60626285042916 18.081084168271268 14.288779739564635 7 25.09829128029798 33.166384202640444 23.410784936687918 18.32453958037366 8 24.13606966075271 35.14222904095712 21.532358367380773 19.189342930909397 9 24.18275536722445 16.800979861342963 22.855582393173606 36.16068237825898 10-11 22.957545216273452 26.938533804465703 29.411448425267135 20.692472553993706 12-13 24.29989390057201 24.784154078170246 29.503677580450027 21.412274440807714 14-15 21.718154751123247 26.815569756539183 27.685382723253465 23.780892769084108 16-17 20.14692208855631 29.81403717298384 28.201552687291926 21.837488051167924 18-19 19.872094871360563 27.698681867180223 31.737117861745688 20.692105399713522 20-21 21.184165566019725 27.214413530597987 31.40820473958644 20.193216163795846 22-23 20.833157915177246 27.26980487300215 31.460006129028784 20.43703108279182 24-25 20.621881024389815 27.153131401742826 31.584977287020326 20.640010286847033 26-27 20.472547140161883 26.92225663137743 31.715333374454612 20.88986285400608 28-29 20.53891231605163 26.98527662382547 31.750514913480504 20.7252961466424 30-31 21.013732549157062 27.50506101777777 31.14528148005275 20.33592495301241 32-33 20.40282862184643 27.266418894640427 31.619693763957983 20.711058719555158 34-35 20.490447951066983 26.767195140378586 31.71296726909341 21.029389639461023 36-37 21.210200883976963 27.322552704588972 30.83777753192039 20.62946887951367 38-39 21.006968751417624 27.410278100601577 30.960007271286543 20.622745876694253 40-41 20.764859060078518 27.130539175035334 31.099232174333334 21.00536959055281 42-43 20.745228544564533 27.228977317045388 30.819387181975046 21.206406956415034 44-45 21.143648051455123 27.46763575815074 30.498747269596006 20.889968920798133 46-47 21.103554804058735 27.87855482853569 29.917174889780284 21.100715477625293 48-49 21.039816821018327 27.566416577488972 29.990377294265507 21.403389307227194 50-51 21.116633655417385 27.44537804978743 29.81177713441469 21.626211160380496 52-53 21.090549383556123 27.659363723264235 29.633658354620124 21.61642853855952 54-55 21.279429863252165 28.170083485987924 29.33810731805384 21.21237933270607 56-57 20.8871540713167 27.83425970437716 29.407393410217068 21.871192814089063 58-59 20.715962268941652 27.311570712119387 29.88657054078072 22.085896478158237 60-61 21.161687565088293 27.74411924909933 29.400539863653584 21.69365332215879 62-63 20.954571756142983 27.717969705366034 29.49266295204443 21.834795586446557 64-65 20.9068253817507 27.39899422572384 29.76681297356783 21.927367418957626 66-67 20.848398897296995 27.510388834332485 29.446124107284767 22.195088161085756 68-69 21.364136435183237 27.697351952787553 29.196059406541615 21.742452205487602 70-71 21.399317974209126 27.38915449101484 29.302028290787497 21.90949924398854 72-73 21.015919156870176 27.51575744580721 29.583178720586456 21.88514467673616 74-75 21.04183209006735 27.500704120319558 29.364607698099316 22.092856091513777 76-77 21.15233736941954 27.632275896370462 29.03876235392563 22.17662438028437 78-79 21.278940324211916 27.69153459719259 29.13333313751772 21.896191941077774 80-81 21.005512058106447 27.376166349635074 29.308895057456503 22.309426534801975 82-83 20.842335811804283 27.465557233325722 29.361097560308956 22.33100939456104 84-85 20.94912107344009 27.366836398800267 29.21095485768177 22.47308767007787 86-87 21.098153838923686 27.49939786324675 29.076588205038494 22.325860092791068 88-89 20.76775881626739 27.383170958986234 29.17367969857908 22.675390526167295 90-91 20.615414289221512 27.188222509366383 29.168652375438032 23.027710825974072 92-93 20.759448744695625 27.438683005354758 28.911457615170537 22.890410634779084 94-95 20.62555629158903 27.183998024866547 29.14997706909875 23.04046861444568 96-97 20.560785637410056 27.178209222475125 29.170892030500784 23.090113109614034 98-99 20.495624074707916 27.210124781081348 29.084056194933776 23.21019494927696 100 20.68642252351748 27.18860064801978 28.88452872356813 23.24044810489461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1573.0 1 1350.0 2 3439.5 3 5093.5 4 2801.0 5 1290.5 6 2671.0 7 6117.0 8 9968.0 9 11547.5 10 8703.5 11 5131.5 12 4416.5 13 4826.0 14 5430.0 15 6166.0 16 7015.0 17 7740.0 18 8732.0 19 10033.0 20 11741.5 21 14133.5 22 17599.0 23 22801.5 24 29875.0 25 38996.5 26 51144.0 27 65273.0 28 81449.0 29 100743.0 30 118990.0 31 134785.0 32 149116.0 33 162576.5 34 175855.5 35 185215.5 36 191459.0 37 198509.5 38 205339.5 39 208500.0 40 209646.5 41 209814.5 42 207674.0 43 204344.5 44 200272.0 45 197204.5 46 194181.0 47 190099.5 48 187145.0 49 185490.0 50 181938.5 51 174430.5 52 163243.5 53 153846.5 54 147930.5 55 141529.5 56 132845.0 57 121815.0 58 111011.5 59 100994.5 60 87774.0 61 72755.0 62 60166.0 63 48725.0 64 38679.0 65 30667.0 66 24150.5 67 18477.5 68 13817.0 69 10599.5 70 8026.5 71 5933.0 72 4477.0 73 3343.0 74 2498.0 75 1854.0 76 1194.5 77 843.0 78 611.0 79 343.0 80 195.0 81 104.0 82 58.5 83 41.5 84 26.5 85 12.0 86 6.0 87 6.0 88 4.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13521068291675192 2 0.0 3 0.0 4 0.0 5 0.014376129815309974 6 2.2845155211616305E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.121335841078657E-4 82-83 6.608777043360431E-4 84-85 4.650620882364748E-4 86-87 6.200827843152998E-4 88-89 6.445597363277457E-4 90-91 8.077394164107194E-4 92-93 3.100413921576499E-4 94-95 3.3451834417009586E-4 96-97 7.098316083609352E-4 98-99 0.0013299143926762348 100 9.790780804978417E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6128214.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.043574423996326 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64771250516267 47.55498055243134 2 14.529239395934368 18.028918915714268 3 4.145102295332586 7.715308882666358 4 1.6648371257800565 4.131697844686683 5 0.8519642832055427 2.642945470582489 6 0.5115448013245266 1.9042840771312013 7 0.3263229550944891 1.4172369785464343 8 0.22817441610371458 1.132540509374618 9 0.1735058912898588 0.9688433111319753 >10 0.8655305000573791 9.29215624369617 >50 0.03980266058457893 1.68123371913508 >100 0.015184314063215729 1.6156631969672763 >500 5.262836106223079E-4 0.21338237597398912 >1k 4.2100758609227865E-4 0.4850635434286225 >5k 5.262594815671755E-5 0.22504715060070066 >10k+ 7.893892223507632E-5 0.9906972279327471 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24511 0.39996971385137664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23295 0.380127064753287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12878 0.2101427920108534 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7044 0.11494376665044662 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6741 0.1099994223439325 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.631796800829736E-5 0.0 2 0.0 1.631796800829736E-5 0.0 1.631796800829736E-5 0.0 3 0.0 1.631796800829736E-5 0.0 1.631796800829736E-5 0.0 4 0.0 1.631796800829736E-5 0.0 4.895390402489208E-5 0.0 5 0.0 1.631796800829736E-5 0.0 4.895390402489208E-5 0.0 6 0.0 1.631796800829736E-5 0.0 4.895390402489208E-5 0.0 7 0.0 1.631796800829736E-5 0.0 4.895390402489208E-5 0.0 8 0.0 1.631796800829736E-5 0.0 4.895390402489208E-5 0.0 9 0.0 1.631796800829736E-5 0.0 6.527187203318944E-5 0.0 10-11 0.0 1.631796800829736E-5 0.0 6.527187203318944E-5 0.0 12-13 0.0 3.263593601659472E-5 0.0 6.527187203318944E-5 0.0 14-15 0.0 4.895390402489208E-5 0.0 1.1422577605808151E-4 0.0 16-17 0.0 4.895390402489208E-5 0.0 1.8765663209541965E-4 0.0 18-19 8.15898400414868E-6 4.895390402489208E-5 0.0 2.3661053612031172E-4 0.0 20-21 1.631796800829736E-5 6.527187203318944E-5 0.0 2.855644401452038E-4 0.0 22-23 1.631796800829736E-5 6.527187203318944E-5 0.0 5.87446848298705E-4 0.0 24-25 1.631796800829736E-5 6.527187203318944E-5 0.0 0.0011993706486098559 0.0 26-27 2.447695201244604E-5 6.527187203318944E-5 0.0 0.0024884901212653475 0.0 28-29 3.263593601659472E-5 7.343085603733812E-5 0.0 0.006780115707447553 0.0 30-31 4.895390402489208E-5 8.974882404563547E-5 0.0 0.018088467537197624 0.0 32-33 4.895390402489208E-5 9.790780804978416E-5 0.0 0.04456437063066009 0.0 34-35 4.895390402489208E-5 9.790780804978416E-5 0.0 0.08869631510910031 0.0 36-37 4.895390402489208E-5 9.790780804978416E-5 0.0 0.15507780896685397 0.0 38-39 4.895390402489208E-5 1.3054374406637888E-4 0.0 0.2409266386585064 0.0 40-41 6.527187203318944E-5 1.3054374406637888E-4 0.0 0.3359380073868178 0.0 42-43 6.527187203318944E-5 1.3054374406637888E-4 0.0 0.4374439273824315 0.0 44-45 6.527187203318944E-5 1.3054374406637888E-4 0.0 0.5640958360788315 0.0 46-47 6.527187203318944E-5 1.3870272807052757E-4 0.0 0.7171338990446483 0.0 48-49 6.527187203318944E-5 1.4686171207467624E-4 0.0 0.8778250890063565 0.0 50-51 6.527187203318944E-5 1.9581561609956832E-4 0.0 1.0396911726646623 0.0 52-53 6.527187203318944E-5 1.9581561609956832E-4 0.0 1.2001294341222417 0.0 54-55 6.527187203318944E-5 2.6924647213690645E-4 0.0 1.3843674519199232 0.0 56-57 6.527187203318944E-5 2.937234241493525E-4 0.0 1.6045947481599043 0.0 58-59 8.15898400414868E-5 3.263593601659472E-4 0.0 1.8471368656512321 0.0 60-61 8.15898400414868E-5 3.263593601659472E-4 0.0 2.095798221145672 0.0 62-63 8.974882404563547E-5 3.5899529618254195E-4 0.0 2.347633421417725 0.0 64-65 9.790780804978416E-5 3.5899529618254195E-4 0.0 2.6286533074726175 0.0 66-67 9.790780804978416E-5 3.5899529618254195E-4 0.0 2.9548250110064695 0.0 68-69 9.790780804978416E-5 3.916312321991367E-4 0.0 3.3083456289222273 0.0 70-71 9.790780804978416E-5 4.07949200207434E-4 0.0 3.6829164255686893 0.0 72-73 9.790780804978416E-5 4.07949200207434E-4 0.0 4.088385294638862 0.0 74-75 1.0606679205393284E-4 4.161081842115827E-4 0.0 4.498765872079533 0.0 76-77 1.1422577605808152E-4 4.569031042323261E-4 0.0 4.936462727966093 0.0 78-79 1.1422577605808152E-4 4.569031042323261E-4 0.0 5.426140470943084 0.0 80-81 1.1422577605808152E-4 4.569031042323261E-4 0.0 5.950281762353599 0.0 82-83 1.1422577605808152E-4 4.650620882364748E-4 0.0 6.486808391482413 0.0 84-85 1.1422577605808152E-4 4.7322107224062344E-4 0.0 7.023473723339296 0.0 86-87 1.1422577605808152E-4 4.7322107224062344E-4 0.0 7.592872899020824 0.0 88 1.1422577605808152E-4 4.7322107224062344E-4 0.0 8.051889180110225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12900 0.0 57.90386 1 GTATCAA 22415 0.0 38.97261 1 GTGGTAT 5005 0.0 36.111656 1 TGGTATC 5325 0.0 33.456974 2 TATAACG 650 0.0 31.097265 2 ATCAACG 27755 0.0 31.061697 3 TCAACGC 28030 0.0 30.807262 4 TATCAAC 28865 0.0 29.94865 2 CAACGCA 29160 0.0 29.645672 5 ATAACGC 635 0.0 28.870743 3 AACGCAG 30370 0.0 28.55204 6 ACGCAGA 34080 0.0 25.37487 7 CGCAGAG 34550 0.0 24.948065 8 TAACGCA 800 0.0 24.678934 4 GCAGAGT 37615 0.0 22.56536 9 GAGTACT 30175 0.0 21.073473 12-13 CAGAGTA 37120 0.0 20.960749 10-11 GTACATG 14665 0.0 20.925982 1 GTATAAC 1755 0.0 20.382467 1 AGAGTAC 35745 0.0 20.202394 10-11 >>END_MODULE