FastQCFastQC Report
Wed 25 May 2016
SRR2049462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5563705
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62840.11294631904459349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59600.045.6055951
GTATCAA100700.035.39781
ATCAACG110300.031.0640543
TCAACGC110450.031.0207524
CAACGCA119950.028.5646885
TATCAAC123900.028.1474292
AACGCAG126250.027.1032716
ACGCAGA142950.023.8058647
GTGGTAT32450.023.7665251
CGCAGAG145950.023.314238
GTACATG162650.023.2743931
TACATGG158700.022.4786872
ACATGGG166300.020.8861473
TGGTATC36300.020.0692522
TCTGTCG14250.019.7889618
GCAGAGT166550.019.5843549
GTATAGG18250.018.8103371
CATGGGG112950.018.1006224
AGAGTAC148550.017.702310-11
GTATTAG25650.017.6003151
Produced by FastQC (version 0.11.5)