##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049462_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5563705
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.938833385307092	31.0	31.0	34.0	28.0	34.0
2	30.997635208912047	31.0	31.0	34.0	28.0	34.0
3	31.166938218327534	33.0	31.0	34.0	28.0	34.0
4	34.723759976490484	37.0	35.0	37.0	32.0	37.0
5	34.76252910605433	37.0	35.0	37.0	32.0	37.0
6	34.80588241109117	37.0	35.0	37.0	32.0	37.0
7	34.64813159576217	37.0	35.0	37.0	32.0	37.0
8	34.644195369812024	37.0	35.0	37.0	32.0	37.0
9	36.19591369420198	39.0	37.0	39.0	32.0	39.0
10-11	36.157681616836264	39.0	37.0	39.0	32.0	39.0
12-13	36.09079345508074	39.0	37.0	39.0	31.5	39.0
14-15	37.322876752092355	40.0	38.0	41.0	31.5	41.0
16-17	37.30572361403058	40.0	38.0	41.0	31.0	41.0
18-19	37.31472507978047	40.0	38.0	41.0	31.0	41.0
20-21	37.3734316431227	40.0	38.0	41.0	31.0	41.0
22-23	37.33361060660118	40.0	38.0	41.0	31.0	41.0
24-25	37.33858984255995	40.0	38.0	41.0	31.0	41.0
26-27	37.34294197841187	40.0	38.0	41.0	31.5	41.0
28-29	37.32474556073696	40.0	38.0	41.0	31.0	41.0
30-31	37.27031016202333	40.0	38.0	41.0	31.0	41.0
32-33	37.23130557784785	40.0	38.0	41.0	31.0	41.0
34-35	37.165914170503285	40.0	37.5	41.0	31.0	41.0
36-37	37.051686690793275	40.0	37.0	41.0	30.5	41.0
38-39	36.94259580621187	40.0	37.0	41.0	30.0	41.0
40-41	36.828697603485445	40.0	37.0	41.0	30.0	41.0
42-43	36.72784035098913	40.0	37.0	41.0	30.0	41.0
44-45	36.5657586086969	40.0	36.5	41.0	30.0	41.0
46-47	36.43990704036249	40.0	36.0	41.0	30.0	41.0
48-49	36.33944305098851	40.0	36.0	41.0	30.0	41.0
50-51	36.226542474843654	40.0	36.0	41.0	29.0	41.0
52-53	36.117740606304615	40.0	35.5	41.0	29.0	41.0
54-55	35.976865955330126	39.0	35.0	41.0	29.0	41.0
56-57	35.81949555197481	39.0	35.0	41.0	28.5	41.0
58-59	35.64499636483242	39.0	35.0	41.0	28.0	41.0
60-61	35.45894669109883	39.0	35.0	41.0	28.0	41.0
62-63	35.21247765652564	38.5	35.0	40.0	28.0	41.0
64-65	34.97963074965334	38.0	34.0	40.0	28.0	41.0
66-67	34.76147090832458	37.5	34.0	40.0	27.5	41.0
68-69	34.48191942239929	37.0	34.0	40.0	27.0	41.0
70-71	34.17289701736523	36.5	34.0	39.5	26.0	41.0
72-73	33.842261227006105	36.0	34.0	39.0	26.0	41.0
74-75	33.54449229425356	36.0	34.0	39.0	26.0	40.5
76-77	33.22251125823529	35.0	33.0	38.5	26.0	40.0
78-79	32.87941272946715	35.0	33.0	37.0	26.0	39.0
80-81	32.55205020755054	35.0	33.0	37.0	25.0	39.0
82-83	32.2568980562413	35.0	33.0	36.5	25.0	39.0
84-85	31.973487720862266	35.0	33.0	36.0	25.0	37.5
86-87	31.703345881925802	35.0	33.0	36.0	24.0	37.0
88-89	31.47832172985448	35.0	33.0	35.0	24.0	37.0
90-91	31.276182597747365	35.0	32.0	35.0	23.0	36.0
92-93	31.07755398605785	35.0	32.0	35.0	22.0	36.0
94-95	30.88440715314705	35.0	32.0	35.0	20.0	36.0
96-97	30.70399769578006	35.0	32.0	35.0	18.5	36.0
98-99	30.53833848128181	35.0	32.0	35.0	16.5	35.0
100	30.41192316990207	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25829935832271644
1101	2	0.21392849538623082
1101	3	0.3226650940238045
1101	4	0.3580199831943389
1101	5	0.33561019078671706
1101	6	0.3362821488727761
1101	7	0.36828152200983766
1101	8	0.40510990655459267
1101	9	0.4980829835240925
1101	10-11	0.526903662251712
1101	12-13	0.4698166759007023
1101	14-15	0.6050943590802547
1101	16-17	0.5352081363115957
1101	18-19	0.5550108568342722
1101	20-21	0.5915475657676481
1101	22-23	0.5443903871439844
1101	24-25	0.5348696865901843
1101	26-27	0.5782788153521494
1101	28-29	0.595847002149597
1101	30-31	0.5503838247618233
1101	32-33	0.5761587990945003
1101	34-35	0.5787864473918916
1101	36-37	0.514701362962164
1101	38-39	0.5387113645562991
1101	40-41	0.5676014227509896
1101	42-43	0.4478495194753833
1101	44-45	0.4348199723721464
1101	46-47	0.46925706880985985
1101	48-49	0.43016325223840113
1101	50-51	0.43127283062221977
1101	52-53	0.44788479195890574
1101	54-55	0.3370982170277159
1101	56-57	0.33369821379926634
1101	58-59	0.351879831302341
1101	60-61	0.34277749707907645
1101	62-63	0.3617711192159234
1101	64-65	0.3945618965758939
1101	66-67	0.36264761691891323
1101	68-69	0.34636753117471386
1101	70-71	0.36419668500855096
1101	72-73	0.3601482447930593
1101	74-75	0.3319915666908173
1101	76-77	0.3287239781412268
1101	78-79	0.2713711044705249
1101	80-81	0.3460576031143674
1101	82-83	0.2739314082340698
1101	84-85	0.2679073693116223
1101	86-87	0.298133265594565
1101	88-89	0.3189003091017142
1101	90-91	0.326489876019302
1101	92-93	0.24928890887725075
1101	94-95	0.2386135110744334
1101	96-97	0.294811250090369
1101	98-99	0.3077443892476346
1101	100	0.21982237139585692
1102	1	0.29696477004389976
1102	2	0.3366752891590501
1102	3	0.41216306667903524
1102	4	0.43625034114801764
1102	5	0.4200448550836029
1102	6	0.4226978451338752
1102	7	0.41794632875702575
1102	8	0.4793762103060928
1102	9	0.5850582755387919
1102	10-11	0.6006535245766926
1102	12-13	0.5513963271064384
1102	14-15	0.6683218863454528
1102	16-17	0.592150387705118
1102	18-19	0.6505922829488355
1102	20-21	0.6506377725200494
1102	22-23	0.6553080407614473
1102	24-25	0.5864876614689862
1102	26-27	0.6092670229628325
1102	28-29	0.6035812838501045
1102	30-31	0.5861625607323759
1102	32-33	0.5740221363758451
1102	34-35	0.593474165824432
1102	36-37	0.6059653834023848
1102	38-39	0.6469770303125628
1102	40-41	0.6920544349489788
1102	42-43	0.6407638472242567
1102	44-45	0.6103652762294942
1102	46-47	0.5790645823390506
1102	48-49	0.5411839760763897
1102	50-51	0.5064288905835213
1102	52-53	0.453013900498334
1102	54-55	0.430023276678817
1102	56-57	0.4734360438636642
1102	58-59	0.5238996247998386
1102	60-61	0.47545849136307794
1102	62-63	0.38686585797744044
1102	64-65	0.47716256687240843
1102	66-67	0.49818764810839866
1102	68-69	0.4329755398009425
1102	70-71	0.36158625060205907
1102	72-73	0.4326948135645665
1102	74-75	0.5381259850037168
1102	76-77	0.4476856000558058
1102	78-79	0.5072532706415629
1102	80-81	0.4476867183341895
1102	82-83	0.4193184053706389
1102	84-85	0.3785932079565626
1102	86-87	0.43360818887210684
1102	88-89	0.41183508648076383
1102	90-91	0.3793919755262074
1102	92-93	0.39580951402569653
1102	94-95	0.39295507001883223
1102	96-97	0.4104005608042236
1102	98-99	0.3960425210416929
1102	100	0.3972280360541838
1103	1	0.3087783431936657
1103	2	0.3021519858693793
1103	3	0.3737963138992768
1103	4	0.327919165970755
1103	5	0.3674364703541855
1103	6	0.3809041536638702
1103	7	0.43068631038339333
1103	8	0.43985504385737073
1103	9	0.5044853170745043
1103	10-11	0.4844789174943145
1103	12-13	0.5355471353225667
1103	14-15	0.611818229736329
1103	16-17	0.4786109624567132
1103	18-19	0.5178911627859009
1103	20-21	0.5169101294183136
1103	22-23	0.5319724520218045
1103	24-25	0.5449184115694337
1103	26-27	0.5466514883422775
1103	28-29	0.53285714388862
1103	30-31	0.5364968284673566
1103	32-33	0.5654995847106647
1103	34-35	0.5910506697927786
1103	36-37	0.581653988391615
1103	38-39	0.6290628028951417
1103	40-41	0.5895699310424547
1103	42-43	0.522739786981326
1103	44-45	0.5011460264897991
1103	46-47	0.43988646279105836
1103	48-49	0.4430388312880922
1103	50-51	0.4666686284181907
1103	52-53	0.43840451608970454
1103	54-55	0.44113228514198966
1103	56-57	0.4294389349934846
1103	58-59	0.44658231804007187
1103	60-61	0.4436130001507692
1103	62-63	0.4243269512193706
1103	64-65	0.45261966965712475
1103	66-67	0.428911237873308
1103	68-69	0.3877108139826788
1103	70-71	0.4141833782828286
1103	72-73	0.4265024043589918
1103	74-75	0.3800280971724135
1103	76-77	0.3849219586443695
1103	78-79	0.39797906785376114
1103	80-81	0.4204530867490135
1103	82-83	0.3621082190871334
1103	84-85	0.4120235450765293
1103	86-87	0.4396195527627995
1103	88-89	0.4283443476347024
1103	90-91	0.40352195886843845
1103	92-93	0.39569860878313534
1103	94-95	0.4597291848817555
1103	96-97	0.4548159123278843
1103	98-99	0.44442661500514546
1103	100	0.38474749942468023
1104	1	0.14023779051128216
1104	2	0.12222272334014761
1104	3	0.22583831529240328
1104	4	0.2566855334445677
1104	5	0.21074726138798638
1104	6	0.2535491063270783
1104	7	0.21793483847083905
1104	8	0.24664236332944967
1104	9	0.28229899533119607
1104	10-11	0.34968290983720607
1104	12-13	0.34195015890070124
1104	14-15	0.43091914434653233
1104	16-17	0.35948404779755805
1104	18-19	0.36059478670446765
1104	20-21	0.3929291923813949
1104	22-23	0.39743775154950356
1104	24-25	0.4271074346661976
1104	26-27	0.47112453319468983
1104	28-29	0.3856910982315398
1104	30-31	0.39609334518745243
1104	32-33	0.41985344875519814
1104	34-35	0.3872772461644658
1104	36-37	0.4248407071153224
1104	38-39	0.4215135079751633
1104	40-41	0.423365622033117
1104	42-43	0.42097439066893116
1104	44-45	0.40085579353343803
1104	46-47	0.3769371827100443
1104	48-49	0.31924628146539646
1104	50-51	0.2822006395203829
1104	52-53	0.31789534092165184
1104	54-55	0.35878061991502364
1104	56-57	0.3452197791211802
1104	58-59	0.34536694482750363
1104	60-61	0.40070644964272617
1104	62-63	0.4227612434418191
1104	64-65	0.425279142573217
1104	66-67	0.3811388409887755
1104	68-69	0.37819035967596193
1104	70-71	0.40105810228329375
1104	72-73	0.33783863604868714
1104	74-75	0.35206159930952907
1104	76-77	0.3503033938317657
1104	78-79	0.3214701152700954
1104	80-81	0.34274516157204715
1104	82-83	0.3139820583592794
1104	84-85	0.30055768921534565
1104	86-87	0.2753728963729216
1104	88-89	0.2498773408618149
1104	90-91	0.2447232337950851
1104	92-93	0.20347755061747463
1104	94-95	0.23382996818502377
1104	96-97	0.32212244111291355
1104	98-99	0.3159661990757492
1104	100	0.3577441634635079
1105	1	0.23554412856336882
1105	2	0.2282497354983377
1105	3	0.2526984575403013
1105	4	0.20815716135499684
1105	5	0.2641437865085905
1105	6	0.26074640041864683
1105	7	0.2781711547208019
1105	8	0.3133844925528777
1105	9	0.30751993401418787
1105	10-11	0.34751498470659925
1105	12-13	0.3111552766883534
1105	14-15	0.38477664144298274
1105	16-17	0.40629957009456774
1105	18-19	0.4192112718514238
1105	20-21	0.39237404399914766
1105	22-23	0.34382515015666115
1105	24-25	0.3541122452112404
1105	26-27	0.36257125938755763
1105	28-29	0.384561135716865
1105	30-31	0.3694640516787473
1105	32-33	0.37789914862436547
1105	34-35	0.36725149236730203
1105	36-37	0.38522550440990955
1105	38-39	0.3509273574793852
1105	40-41	0.37974427138815514
1105	42-43	0.3532706463965454
1105	44-45	0.3093821465648361
1105	46-47	0.23847026531792892
1105	48-49	0.2584470987208718
1105	50-51	0.2940023587453311
1105	52-53	0.26733500596793647
1105	54-55	0.23735694618000736
1105	56-57	0.22171219962616817
1105	58-59	0.1735531976963216
1105	60-61	0.16881165924504415
1105	62-63	0.19722353799338066
1105	64-65	0.1862644244417666
1105	66-67	0.18674490730156634
1105	68-69	0.1660656837595056
1105	70-71	0.1760836183625898
1105	72-73	0.17138757227669998
1105	74-75	0.21557467101325756
1105	76-77	0.15709678366992108
1105	78-79	0.08623711780843024
1105	80-81	0.13261012239181724
1105	82-83	0.10362293150623714
1105	84-85	0.07785802628074379
1105	86-87	0.09070633117370264
1105	88-89	0.07084893388636715
1105	90-91	0.06076330808654973
1105	92-93	0.0056476985644273725
1105	94-95	0.026851081235609087
1105	96-97	0.0614242112774086
1105	98-99	0.043998768892251405
1105	100	-0.04015299052888821
1106	1	0.14642377741543555
1106	2	0.19508950136181014
1106	3	0.23553338817718128
1106	4	0.2766865184191829
1106	5	0.2730085182168054
1106	6	0.2596109839997993
1106	7	0.3095535947207324
1106	8	0.27022149188152156
1106	9	0.33891224915198137
1106	10-11	0.36521479278479774
1106	12-13	0.36279966668986674
1106	14-15	0.4216282619605778
1106	16-17	0.42737827628991454
1106	18-19	0.43975621936431963
1106	20-21	0.4133517993533715
1106	22-23	0.4118240926191703
1106	24-25	0.4663974703142557
1106	26-27	0.4052515154409022
1106	28-29	0.4134118596916494
1106	30-31	0.40983333844393144
1106	32-33	0.3931272321398964
1106	34-35	0.4019405923547268
1106	36-37	0.4212380075314215
1106	38-39	0.4014242489804971
1106	40-41	0.5095594073112011
1106	42-43	0.4279690320060112
1106	44-45	0.4632598139555313
1106	46-47	0.3976701083331591
1106	48-49	0.3379451828693192
1106	50-51	0.3818330468135187
1106	52-53	0.3817472011048437
1106	54-55	0.3331975830007181
1106	56-57	0.3004953237936121
1106	58-59	0.3645352966149815
1106	60-61	0.3807634937090043
1106	62-63	0.37922986975223694
1106	64-65	0.36669902327401616
1106	66-67	0.4209206347812753
1106	68-69	0.39909466085531875
1106	70-71	0.3799382961331048
1106	72-73	0.364261612948205
1106	74-75	0.355679580886914
1106	76-77	0.3334045623908253
1106	78-79	0.3257095924326947
1106	80-81	0.30109477949486774
1106	82-83	0.33309563954285437
1106	84-85	0.3645108647619395
1106	86-87	0.35121927575877265
1106	88-89	0.3282421578360939
1106	90-91	0.3774890385512464
1106	92-93	0.2691043095278367
1106	94-95	0.33629247610780766
1106	96-97	0.40650117825150645
1106	98-99	0.45424578050346653
1106	100	0.3946007467245174
1107	1	0.24850696293285068
1107	2	0.21167648071153167
1107	3	0.27401490807775986
1107	4	0.2232406315996016
1107	5	0.2169568525934622
1107	6	0.23011646957579046
1107	7	0.19323370473666301
1107	8	0.2463598673149221
1107	9	0.29443811125594266
1107	10-11	0.31409109492111753
1107	12-13	0.31948670273300195
1107	14-15	0.30169888968784164
1107	16-17	0.2955317301676388
1107	18-19	0.32674420579679975
1107	20-21	0.30369586626411404
1107	22-23	0.37998050245404613
1107	24-25	0.35601359304652647
1107	26-27	0.40864943586003477
1107	28-29	0.3370998311666398
1107	30-31	0.36637569006444437
1107	32-33	0.38972984627802276
1107	34-35	0.37073542247524927
1107	36-37	0.3569761781513776
1107	38-39	0.3847707822661661
1107	40-41	0.4230678483097847
1107	42-43	0.4770829462872115
1107	44-45	0.4085729741382593
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2216	100	-0.9422425246800188
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94803.0
3	18614.0
4	11158.0
5	9027.0
6	7378.0
7	7137.0
8	7515.0
9	8569.0
10	9727.0
11	10871.0
12	12257.0
13	14061.0
14	15632.0
15	16609.0
16	17086.0
17	18171.0
18	17505.0
19	15480.0
20	15613.0
21	16778.0
22	19311.0
23	23392.0
24	28865.0
25	34990.0
26	42843.0
27	48578.0
28	57595.0
29	73290.0
30	87705.0
31	101938.0
32	129713.0
33	174199.0
34	240496.0
35	354811.0
36	592160.0
37	1082216.0
38	1516207.0
39	609903.0
40	1502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06521475179091	20.70305297855405	14.533527001929192	22.69820526772585
2	15.424778161328032	20.867063255592054	40.52900813760496	23.179150445474956
3	17.902271560009712	26.13409468461681	30.67787984376767	25.285753911605806
4	12.554800840322994	17.91319909999804	39.25346286305529	30.278537196623677
5	12.772184165526584	38.05048217830379	35.353900306496755	13.823433349672875
6	29.476969269711212	38.64998617728379	18.40062365794727	13.472420895057724
7	26.011491798044645	33.026227416937765	23.21091882609784	17.75136195891975
8	23.998648255134654	36.54451231130397	21.294834968489244	18.162004465072133
9	24.295400141609324	17.930170677163275	22.340443698430022	35.43398548279738
10-11	23.027639631749604	27.72100937739194	29.40674145427052	19.844609536587935
12-13	23.646334361616805	25.43352705066243	29.276076270884037	21.644062316836727
14-15	21.28363387747556	27.76452841231678	26.521907200395745	24.429930509811914
16-17	20.7089802426393	30.871743432938043	27.09062033187189	21.328655992550768
18-19	20.58832094063476	29.783549892490356	29.12631385193688	20.501815314938
20-21	21.790306229708996	29.115465324288873	28.55255502709032	20.541673418911806
22-23	21.480221824751254	29.037569994337538	28.57539614772737	20.906812033183833
24-25	21.240524123599407	29.063306134849114	28.586658541276798	21.10951120027468
26-27	21.045428960219564	28.990425909477484	28.681352349161273	21.282792781141676
28-29	21.06930937967962	28.999246003138047	28.811263593222424	21.120181023959915
30-31	21.390315021937777	29.39216398422432	28.35630650100065	20.86121449283725
32-33	20.989076185916325	29.161296016855566	28.746486064832123	21.10314173239599
34-35	21.21775371576723	28.78554024077968	28.77409902199599	21.222607021457094
36-37	21.524894456821578	29.147308545591095	28.261385774832355	21.066411222754976
38-39	21.30898056880145	29.274866827747648	28.383723048815156	21.032429554635744
40-41	21.221700248707144	28.942820726020653	28.552049144265336	21.283429881006878
42-43	21.14441527585082	29.00535774807233	28.48329794186008	21.36692903421677
44-45	21.35879812794677	29.147008061312828	28.33489931021812	21.15929450052229
46-47	21.31950065368759	29.22685094259998	28.20759266341853	21.246055740293894
48-49	21.20888237726284	28.976396354288386	28.38409405504521	21.430627213403564
50-51	21.293431943563267	28.922782373075368	28.292640750069047	21.491144933292315
52-53	21.280339824367992	29.00300892899995	28.2489072207645	21.46774402586755
54-55	21.408909186687065	29.319310224390176	28.11223076958337	21.15954981933939
56-57	21.255684658805414	29.086891482858878	28.13108139617036	21.526342462165346
58-59	21.187285227536925	28.727423643522354	28.486050782125176	21.59924034681554
60-61	21.442249714490575	29.15270725485577	28.090981587102547	21.3140614435511
62-63	21.256108164874746	29.193158675576026	28.143344381045583	21.40738877850365
64-65	21.34014476341424	28.9014272289767	28.28031016570972	21.478117841899337
66-67	21.183888011011373	28.948015320218467	28.17984727777428	21.68824939099588
68-69	21.581932512340465	29.10370376162714	28.10346081273998	21.210902913292415
70-71	21.558392363415198	28.893670703562584	28.169497851329588	21.378439081692623
72-73	21.2942154224839	29.036009813454832	28.39875906213804	21.271015701923233
74-75	21.364009476243055	29.050072790461627	28.20367918120888	21.382238552086438
76-77	21.434016664914076	29.06338725028918	27.954490098747392	21.54810598604936
78-79	21.508081205271377	29.126088373085867	28.144470783853322	21.221359637789437
80-81	21.354891232097067	29.00216742112481	28.22819502709299	21.414746319685133
82-83	21.265724987466385	29.093161456117254	28.32106576241304	21.320047794003315
84-85	21.358871379080536	28.954934525839814	28.190015499528364	21.49617859555129
86-87	21.48809536114976	29.156354430352437	28.059086046812844	21.296464161684963
88-89	21.268999206164306	29.045326292355877	28.164844583653313	21.5208299178265
90-91	21.188342449642334	28.979903673173695	28.2109698611274	21.620784016056568
92-93	21.341755125654167	29.17604777266158	28.03961989590466	21.442577205779592
94-95	21.230245259527415	29.001219160997206	28.198041576589805	21.570494002885575
96-97	21.27910378607063	29.048882721106533	28.155634839921028	21.516378652901807
98-99	21.23313286228704	29.099282375669528	28.08027290306432	21.58731185897911
100	21.32672312699836	29.072337433286194	28.0606925797492	21.54024685996625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5824.0
1	4109.0
2	2975.0
3	3466.0
4	3021.0
5	2479.5
6	2129.5
7	2013.0
8	2093.0
9	2052.0
10	1751.5
11	1489.0
12	1484.0
13	1535.5
14	1624.5
15	1857.0
16	2143.5
17	2522.5
18	3319.5
19	4463.5
20	6079.0
21	8627.0
22	12591.5
23	18804.5
24	26681.5
25	36213.5
26	48817.5
27	63236.0
28	80259.5
29	99535.0
30	116484.0
31	131542.5
32	145276.0
33	157090.0
34	167744.5
35	176587.0
36	184019.5
37	191093.0
38	197144.0
39	199717.5
40	200395.0
41	198652.0
42	193845.0
43	189680.5
44	184990.0
45	180937.0
46	177009.0
47	171195.5
48	168103.0
49	165825.5
50	160498.0
51	152509.0
52	141248.0
53	132097.5
54	127031.0
55	121514.5
56	111829.5
57	100032.5
58	90817.5
59	82998.5
60	73460.0
61	62384.0
62	52218.5
63	43520.5
64	35645.5
65	28452.5
66	22697.0
67	17927.0
68	14136.0
69	11123.5
70	8297.5
71	6308.5
72	5018.5
73	3800.0
74	2892.5
75	2182.5
76	1537.5
77	1164.5
78	844.0
79	539.0
80	361.0
81	247.5
82	165.5
83	122.0
84	95.0
85	67.5
86	49.5
87	39.5
88	38.0
89	28.5
90	20.0
91	15.5
92	16.5
93	19.0
94	13.5
95	12.0
96	9.0
97	7.0
98	7.0
99	7.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07872451900307438
2	0.013767804008300223
3	0.0173265836344666
4	0.010892022492206183
5	0.009238448120452108
6	0.007171480155759516
7	0.020292233322938582
8	0.009436158099683574
9	0.009202500851500933
10-11	0.013498199491166407
12-13	0.015717943348901496
14-15	0.009804617606433124
16-17	0.012851148650045248
18-19	0.008681265451708888
20-21	0.013309476329172737
22-23	0.013570094029068759
24-25	0.016086402855651046
26-27	0.005472971697816473
28-29	0.012302952798539822
30-31	0.010505589350981046
32-33	0.012141190088259533
34-35	0.008744173172373445
36-37	0.010406734361365313
38-39	0.0193845647819214
40-41	0.013237581791270386
42-43	0.008151043234679049
44-45	0.020660692829688132
46-47	0.018557777596044364
48-49	0.0180635026479657
50-51	0.010613431157834571
52-53	0.010712286147450305
54-55	0.01167387559189425
56-57	0.020597785109023572
58-59	0.0020400075129792107
60-61	0.007252361510899661
62-63	0.015529220186907824
64-65	0.02494740465211581
66-67	0.01311176634994127
68-69	0.014495736204561528
70-71	0.025666350031139318
72-73	0.02045399603321887
74-75	0.020903336895108565
76-77	0.009346289927305636
78-79	0.011548060150565136
80-81	0.019833905643811094
82-83	0.011934493291790273
84-85	0.011368323805809257
86-87	0.016832308686387937
88-89	0.015115826593969307
90-91	0.01568199607995032
92-93	0.00789941235202082
94-95	0.015511246552432238
96-97	0.013435291770501852
98-99	0.010020301220140177
100	0.006991743811003638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5563705.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.63299574025929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71976593587611	48.051887730554185
2	14.282100271183046	17.890614508939272
3	4.274971866062423	8.032628840304481
4	1.707108564902982	4.276852938949144
5	0.8523776782632321	2.669348374587656
6	0.4983333006068678	1.872726449648358
7	0.3334609377823764	1.4619960246966515
8	0.23273447394430327	1.1661485852132025
9	0.18045805853412353	1.01723659303169
>10	0.8563830431951999	9.40888036428782
>50	0.04382689407219379	1.874962963035615
>100	0.017990629015223226	1.9426600067438637
>500	4.0216931436436155E-4	0.16471782398436438
>1k	5.7451498431264804E-5	0.05627834189992421
>5k	2.8725749215626466E-5	0.11306045412373832
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6284	0.11294631904459349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.797363447558776E-5	0.0
9	0.0	0.0	0.0	1.797363447558776E-5	0.0
10-11	0.0	0.0	0.0	3.594726895117552E-5	0.0
12-13	0.0	0.0	0.0	3.594726895117552E-5	0.0
14-15	0.0	8.98681723779388E-6	0.0	8.98681723779388E-5	0.0
16-17	8.98681723779388E-6	1.797363447558776E-5	0.0	1.6176271028028985E-4	0.0
18-19	1.797363447558776E-5	1.797363447558776E-5	0.0	2.0669679646925923E-4	0.0
20-21	1.797363447558776E-5	1.797363447558776E-5	0.0	2.4264406542043478E-4	0.0
22-23	1.797363447558776E-5	1.797363447558776E-5	0.0	3.0555178608499196E-4	0.0
24-25	1.797363447558776E-5	1.797363447558776E-5	0.0	5.212353997920451E-4	0.0
26-27	1.797363447558776E-5	2.6960451713381638E-5	0.0	7.459058307368921E-4	0.0
28-29	1.797363447558776E-5	5.392090342676328E-5	0.0	0.0013659962201446698	0.0
30-31	1.797363447558776E-5	5.392090342676328E-5	0.0	0.0032352542056057966	0.0
32-33	1.797363447558776E-5	5.392090342676328E-5	0.0	0.007099585617857165	0.0
34-35	1.797363447558776E-5	5.392090342676328E-5	0.0	0.013462252222215233	0.0
36-37	1.797363447558776E-5	5.392090342676328E-5	0.0	0.023194975290746003	0.0
38-39	1.797363447558776E-5	6.290772066455716E-5	0.0	0.03455431227931747	0.0
40-41	1.797363447558776E-5	7.189453790235104E-5	0.0	0.0471358564122289	0.0
42-43	1.797363447558776E-5	7.189453790235104E-5	0.0	0.05941184875905534	0.0
44-45	1.797363447558776E-5	7.189453790235104E-5	0.0	0.07535446253890168	0.0
46-47	1.797363447558776E-5	7.189453790235104E-5	0.0	0.09410995011417751	0.0
48-49	1.797363447558776E-5	7.189453790235104E-5	0.0	0.1137910798649461	0.0
50-51	1.797363447558776E-5	1.797363447558776E-4	0.0	0.13427203634987836	0.0
52-53	1.797363447558776E-5	1.797363447558776E-4	0.0	0.15388127156274461	8.98681723779388E-6
54-55	1.797363447558776E-5	1.797363447558776E-4	0.0	0.17720206229481972	1.797363447558776E-5
56-57	1.797363447558776E-5	1.797363447558776E-4	0.0	0.20578014111100426	1.797363447558776E-5
58-59	1.797363447558776E-5	2.1568361370705313E-4	0.0	0.235832057954187	1.797363447558776E-5
60-61	1.797363447558776E-5	2.1568361370705313E-4	0.0	0.26743869417950805	1.797363447558776E-5
62-63	1.797363447558776E-5	2.24670430944847E-4	0.0	0.29992603849413296	1.797363447558776E-5
64-65	1.797363447558776E-5	2.3365724818264088E-4	0.0	0.3353790324972298	1.797363447558776E-5
66-67	3.594726895117552E-5	2.3365724818264088E-4	0.0	0.3773744294494406	1.797363447558776E-5
68-69	3.594726895117552E-5	2.3365724818264088E-4	0.0	0.4233689600724697	1.797363447558776E-5
70-71	3.594726895117552E-5	2.3365724818264088E-4	0.0	0.4729582175906163	1.797363447558776E-5
72-73	3.594726895117552E-5	2.3365724818264088E-4	0.0	0.5279934863548661	1.797363447558776E-5
74-75	3.594726895117552E-5	2.3365724818264088E-4	0.0	0.5855181034939847	1.797363447558776E-5
76-77	3.594726895117552E-5	2.4264406542043478E-4	0.0	0.6486325209550111	1.797363447558776E-5
78-79	3.594726895117552E-5	2.5163088265822865E-4	0.0	0.7217223055499886	1.797363447558776E-5
80-81	3.594726895117552E-5	2.8757815160940415E-4	0.0	0.8044189977721681	1.797363447558776E-5
82-83	3.594726895117552E-5	2.8757815160940415E-4	0.0	0.8915821381615309	1.797363447558776E-5
84-85	3.594726895117552E-5	2.8757815160940415E-4	0.0	0.9820344536599263	2.6960451713381638E-5
86-87	3.594726895117552E-5	2.96564968847198E-4	0.0	1.0810332323514635	5.392090342676328E-5
88	3.594726895117552E-5	3.055517860849919E-4	0.0	1.163073886915284	5.392090342676328E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5960	0.0	45.605595	1
GTATCAA	10070	0.0	35.3978	1
ATCAACG	11030	0.0	31.064054	3
TCAACGC	11045	0.0	31.020752	4
CAACGCA	11995	0.0	28.564688	5
TATCAAC	12390	0.0	28.147429	2
AACGCAG	12625	0.0	27.103271	6
ACGCAGA	14295	0.0	23.805864	7
GTGGTAT	3245	0.0	23.766525	1
CGCAGAG	14595	0.0	23.31423	8
GTACATG	16265	0.0	23.274393	1
TACATGG	15870	0.0	22.478687	2
ACATGGG	16630	0.0	20.886147	3
TGGTATC	3630	0.0	20.069252	2
TCTGTCG	1425	0.0	19.788961	8
GCAGAGT	16655	0.0	19.584354	9
GTATAGG	1825	0.0	18.810337	1
CATGGGG	11295	0.0	18.100622	4
AGAGTAC	14855	0.0	17.7023	10-11
GTATTAG	2565	0.0	17.600315	1
>>END_MODULE