FastQCFastQC Report
Wed 25 May 2016
SRR2049462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5563705
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233320.41936083958441356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218390.3925262033123611No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130680.23487945532698085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.10778788595009979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112450.059.5188831
GTATCAA209050.041.4043731
GTGGTAT42600.037.0126231
TGGTATC46550.032.717892
ATCAACG260850.032.7073823
TCAACGC263600.032.2774
TATCAAC271950.031.458812
CAACGCA275100.030.9106395
AACGCAG285500.029.8783326
ACGCAGA319200.026.6208157
CGCAGAG322450.026.3379258
GCAGAGT348050.024.1441389
GTACATG152500.022.2217031
TACATGG152450.021.55312
CAGAGTA342700.021.51764110-11
GAGTACT273150.021.48192212-13
AGAGTAC330600.021.28162410-11
ACATGGG157300.020.0817013
AGTACTT284650.019.76372512-13
GTACTTT302650.019.29485714-15
Produced by FastQC (version 0.11.5)