##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049462_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5563705
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96973725242442	33.0	31.0	34.0	30.0	34.0
2	32.19666229607788	33.0	31.0	34.0	30.0	34.0
3	32.201774896404466	34.0	31.0	34.0	30.0	34.0
4	35.88182173569591	37.0	35.0	37.0	35.0	37.0
5	35.922375287690485	37.0	35.0	37.0	35.0	37.0
6	35.97833512021216	37.0	35.0	37.0	35.0	37.0
7	35.9497989199643	37.0	35.0	37.0	35.0	37.0
8	35.96033218871238	37.0	35.0	37.0	35.0	37.0
9	37.69215064421999	39.0	37.0	39.0	35.0	39.0
10-11	37.66475253450713	39.0	37.0	39.0	35.0	39.0
12-13	37.64023020631036	39.0	37.0	39.0	35.0	39.0
14-15	38.94710098756134	40.0	38.0	41.0	36.0	41.0
16-17	38.95374188602739	40.0	38.0	41.0	36.0	41.0
18-19	39.037594282946344	40.0	38.5	41.0	36.0	41.0
20-21	39.061146843694985	40.0	39.0	41.0	36.0	41.0
22-23	39.024103272908974	40.0	39.0	41.0	35.0	41.0
24-25	38.99729604642949	40.0	39.0	41.0	35.0	41.0
26-27	38.892341074877265	40.0	38.0	41.0	35.0	41.0
28-29	38.80644291888229	40.0	38.0	41.0	35.0	41.0
30-31	38.67777542123459	40.0	38.0	41.0	35.0	41.0
32-33	38.516683307256585	40.0	38.0	41.0	34.5	41.0
34-35	38.3854471076378	40.0	38.0	41.0	34.0	41.0
36-37	38.237576489048216	40.0	38.0	41.0	34.0	41.0
38-39	38.12020542066842	40.0	38.0	41.0	34.0	41.0
40-41	38.01752725926339	40.0	38.0	41.0	33.5	41.0
42-43	37.86275027162655	40.0	38.0	41.0	33.0	41.0
44-45	37.66684772107795	40.0	37.5	41.0	33.0	41.0
46-47	37.38759747326647	40.0	37.0	41.0	33.0	41.0
48-49	37.11639384187336	40.0	37.0	41.0	32.0	41.0
50-51	36.95310597883964	40.0	37.0	41.0	31.0	41.0
52-53	36.836808475647075	40.0	36.5	41.0	31.0	41.0
54-55	36.69556985857446	40.0	36.0	41.0	31.0	41.0
56-57	36.55134537147458	40.0	36.0	41.0	31.0	41.0
58-59	36.380092042982156	39.0	35.0	41.0	31.0	41.0
60-61	36.19423432766475	39.0	35.0	41.0	30.5	41.0
62-63	35.97808402853854	39.0	35.0	41.0	30.0	41.0
64-65	35.76237525174322	38.5	35.0	41.0	30.0	41.0
66-67	35.50929461572819	38.0	35.0	40.0	29.5	41.0
68-69	35.220695831285084	37.0	35.0	40.0	29.0	41.0
70-71	34.93847813642168	37.0	35.0	40.0	29.0	41.0
72-73	34.61238140771303	36.0	34.5	39.0	29.0	41.0
74-75	34.29283274364835	36.0	34.0	39.0	29.0	41.0
76-77	33.977832397655874	35.5	34.0	39.0	29.0	40.0
78-79	33.668336477221565	35.0	34.0	37.5	28.0	39.5
80-81	33.359663569509884	35.0	34.0	37.0	28.0	39.0
82-83	33.0802922692702	35.0	34.0	36.5	28.0	39.0
84-85	32.79476149436392	35.0	34.0	36.0	27.5	37.5
86-87	32.54427786879427	35.0	34.0	36.0	27.0	37.0
88-89	32.323255456570756	35.0	34.0	35.5	27.0	37.0
90-91	32.128869701035555	35.0	33.0	35.0	26.0	36.0
92-93	31.961871091296178	35.0	33.0	35.0	26.0	36.0
94-95	31.797826448382867	35.0	33.0	35.0	26.0	36.0
96-97	31.654240294911396	35.0	33.0	35.0	26.0	36.0
98-99	31.52331602771894	35.0	33.0	35.0	25.0	35.5
100	31.416695349591684	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22091188242128723
1101	2	0.30255234409010967
1101	3	0.3968614737302971
1101	4	0.20503022059417475
1101	5	0.18224503176008966
1101	6	0.17537486796315704
1101	7	0.1902358927992509
1101	8	0.17310619162950758
1101	9	0.2482152887807061
1101	10-11	0.27169484451283665
1101	12-13	0.2495887357320612
1101	14-15	0.32115206694405884
1101	16-17	0.3245278683937016
1101	18-19	0.3278038441710507
1101	20-21	0.3161851329460319
1101	22-23	0.35058478636312174
1101	24-25	0.3357230507465161
1101	26-27	0.29592847884812556
1101	28-29	0.31693799301464054
1101	30-31	0.3123587168518682
1101	32-33	0.32627554399837067
1101	34-35	0.3781388608783516
1101	36-37	0.3575838717069857
1101	38-39	0.3474614103986653
1101	40-41	0.35344781887845045
1101	42-43	0.2913180965476201
1101	44-45	0.3603902376902326
1101	46-47	0.3673257994407422
1101	48-49	0.29787494576125795
1101	50-51	0.2578555447121218
1101	52-53	0.27202295738113946
1101	54-55	0.21035032183692692
1101	56-57	0.23058702167629974
1101	58-59	0.23452638991508934
1101	60-61	0.16870630384249097
1101	62-63	0.14015929547036166
1101	64-65	0.17944808235944976
1101	66-67	0.21177216933386944
1101	68-69	0.17876147314213142
1101	70-71	0.16670967016653293
1101	72-73	0.20423978475297844
1101	74-75	0.156756648700771
1101	76-77	0.1410824599883611
1101	78-79	0.1538745794297256
1101	80-81	0.13378294694756931
1101	82-83	0.1169813469010279
1101	84-85	0.1254532397923569
1101	86-87	0.06154742165533378
1101	88-89	0.09839143712227383
1101	90-91	0.14192958246712806
1101	92-93	0.1883468021620871
1101	94-95	0.17277290642188703
1101	96-97	0.14521408699383542
1101	98-99	0.17846818943914755
1101	100	0.17646437438043705
1102	1	0.2673398983038773
1102	2	0.2760673904558715
1102	3	0.33284745084983314
1102	4	0.2348580726443501
1102	5	0.20810400103088966
1102	6	0.19126009084854445
1102	7	0.22580182523834935
1102	8	0.18106593673324767
1102	9	0.25817863382238926
1102	10-11	0.30178716067851497
1102	12-13	0.2847320724789739
1102	14-15	0.3451435623485324
1102	16-17	0.3354157734758516
1102	18-19	0.3194888871218424
1102	20-21	0.32196496581703826
1102	22-23	0.29313299027431583
1102	24-25	0.28087706816774727
1102	26-27	0.32179108253187394
1102	28-29	0.32789339433724507
1102	30-31	0.3274104996214078
1102	32-33	0.30712388382276856
1102	34-35	0.2844284651627902
1102	36-37	0.28571183267186484
1102	38-39	0.24912263306087112
1102	40-41	0.296963996758123
1102	42-43	0.2712104732730083
1102	44-45	0.27174355704111974
1102	46-47	0.2559884317013541
1102	48-49	0.27788188437478567
1102	50-51	0.23198242719092121
1102	52-53	0.2474567314891587
1102	54-55	0.23301696122818782
1102	56-57	0.1975842935821035
1102	58-59	0.2203539100914398
1102	60-61	0.18776105499284768
1102	62-63	0.18503063171385747
1102	64-65	0.15561979805642068
1102	66-67	0.18659424578267192
1102	68-69	0.1999729974294624
1102	70-71	0.1679235900511955
1102	72-73	0.21276828086958943
1102	74-75	0.16534794228110172
1102	76-77	0.11585340370833563
1102	78-79	0.10321656550416947
1102	80-81	0.15877722015117257
1102	82-83	0.10830931743306849
1102	84-85	0.0879963396032366
1102	86-87	0.1225320834635184
1102	88-89	0.06137470760795338
1102	90-91	0.10035452031347347
1102	92-93	0.0855350489690423
1102	94-95	0.11685787858124641
1102	96-97	0.11129545222333093
1102	98-99	0.18290978819594272
1102	100	0.14692419476844165
1103	1	0.2870704405285984
1103	2	0.27552625280388554
1103	3	0.3628342967553593
1103	4	0.19784682838478318
1103	5	0.20170680013266917
1103	6	0.19549693755338637
1103	7	0.18999508518371755
1103	8	0.18361109548294507
1103	9	0.22161660575250863
1103	10-11	0.204429842770935
1103	12-13	0.21050102881601873
1103	14-15	0.33728754119967164
1103	16-17	0.3120143343321189
1103	18-19	0.2944832186316688
1103	20-21	0.2990431502162849
1103	22-23	0.29137942185650445
1103	24-25	0.30130038515238056
1103	26-27	0.3013107142918301
1103	28-29	0.30518556257536744
1103	30-31	0.32049873836356824
1103	32-33	0.2955408470360723
1103	34-35	0.2781213206537245
1103	36-37	0.3383557204008625
1103	38-39	0.30873809760796433
1103	40-41	0.2694709201760759
1103	42-43	0.3484880473137437
1103	44-45	0.28756284104486696
1103	46-47	0.2290445858565846
1103	48-49	0.20556617092035623
1103	50-51	0.2505880971731571
1103	52-53	0.2283711715249268
1103	54-55	0.21367688538898477
1103	56-57	0.20228937356176857
1103	58-59	0.19578443558378922
1103	60-61	0.16459837851697046
1103	62-63	0.23235702937303415
1103	64-65	0.21823733096963593
1103	66-67	0.27688293645869066
1103	68-69	0.24135034462402416
1103	70-71	0.1556075129075012
1103	72-73	0.28724411538324546
1103	74-75	0.2768020825303381
1103	76-77	0.1907683963386333
1103	78-79	0.2284810728424418
1103	80-81	0.23315876855621553
1103	82-83	0.22016238350674655
1103	84-85	0.2151094008703609
1103	86-87	0.18710604480428117
1103	88-89	0.20514027430777304
1103	90-91	0.22954933626272833
1103	92-93	0.2500774639296992
1103	94-95	0.26060895765928294
1103	96-97	0.2627609734153573
1103	98-99	0.2878991519502172
1103	100	0.2954800020200601
1104	1	0.2683113319063999
1104	2	0.2445469936911806
1104	3	0.2993245081416873
1104	4	0.18770387719628445
1104	5	0.1576886343911852
1104	6	0.15227113907970846
1104	7	0.171296466991663
1104	8	0.1415149828822706
1104	9	0.21077760029931625
1104	10-11	0.21884278728954598
1104	12-13	0.20152428722705906
1104	14-15	0.2570334405986685
1104	16-17	0.26224023963610676
1104	18-19	0.19637797140237723
1104	20-21	0.24574368727441964
1104	22-23	0.2394315917610612
1104	24-25	0.24939191526832616
1104	26-27	0.2893098973841859
1104	28-29	0.22907352664979186
1104	30-31	0.22751372877178255
1104	32-33	0.2437229215735357
1104	34-35	0.2687582434513587
1104	36-37	0.2919961479464561
1104	38-39	0.3049638517177371
1104	40-41	0.31002306075966857
1104	42-43	0.2883504886335615
1104	44-45	0.2782362238277969
1104	46-47	0.23032329873241508
1104	48-49	0.15035768991983645
1104	50-51	0.18514634365693183
1104	52-53	0.16730936496580284
1104	54-55	0.16166945724064874
1104	56-57	0.11834946803137569
1104	58-59	0.169718924384199
1104	60-61	0.12317225001667964
1104	62-63	0.10459859564301155
1104	64-65	0.10282576530137533
1104	66-67	0.1103371337933936
1104	68-69	0.12813103118339342
1104	70-71	0.13547057379211225
1104	72-73	0.217468882268804
1104	74-75	0.20521127600487432
1104	76-77	0.2291218149594343
1104	78-79	0.23011966871996492
1104	80-81	0.2089937340900505
1104	82-83	0.17103009928612067
1104	84-85	0.15877967971020723
1104	86-87	0.15104348609860807
1104	88-89	0.18176712905017212
1104	90-91	0.18491670503482993
1104	92-93	0.13475153460802147
1104	94-95	0.12933302772568567
1104	96-97	0.1609088571554267
1104	98-99	0.19278779856576378
1104	100	0.16150899714207156
1105	1	0.2117431874579161
1105	2	0.20487700782458518
1105	3	0.2695298161036277
1105	4	0.16084839668822326
1105	5	0.15795540322508117
1105	6	0.12130956107768753
1105	7	0.10217925850322018
1105	8	0.07794002735403183
1105	9	0.13372490658550618
1105	10-11	0.15919124153688813
1105	12-13	0.17397076520542498
1105	14-15	0.15995809576884312
1105	16-17	0.18404358275189026
1105	18-19	0.1930859186500058
1105	20-21	0.22158261521008882
1105	22-23	0.17108960590928746
1105	24-25	0.23790521851542934
1105	26-27	0.17035185180780843
1105	28-29	0.21361448728927002
1105	30-31	0.22396423066518878
1105	32-33	0.20213569486417526
1105	34-35	0.22327766129575366
1105	36-37	0.2214421202341157
1105	38-39	0.22911066071051067
1105	40-41	0.288143478130479
1105	42-43	0.23369689912476588
1105	44-45	0.20226012960014117
1105	46-47	0.26883501204837756
1105	48-49	0.27219980883186423
1105	50-51	0.1905703705727717
1105	52-53	0.141671740835406
1105	54-55	0.19368512871334076
1105	56-57	0.1915207385005786
1105	58-59	0.17561977627856606
1105	60-61	0.18203137717844697
1105	62-63	0.19304834795334358
1105	64-65	0.19433817263000464
1105	66-67	0.15392639252736018
1105	68-69	0.21255074027847343
1105	70-71	0.21439494061360875
1105	72-73	0.22273285097155338
1105	74-75	0.2200788918552803
1105	76-77	0.21862891778383187
1105	78-79	0.21457066704235217
1105	80-81	0.22044934228656388
1105	82-83	0.18386502676982275
1105	84-85	0.20021262135448836
1105	86-87	0.16672604199163032
1105	88-89	0.14144693853010892
1105	90-91	0.1461492443493384
1105	92-93	0.13735301697954583
1105	94-95	0.13482937335387035
1105	96-97	0.1736285377861151
1105	98-99	0.19822929928787048
1105	100	0.16686891741705878
1106	1	0.10718054459571746
1106	2	0.12106298719363195
1106	3	0.21122378528212238
1106	4	0.14081727803410615
1106	5	0.1638109260679954
1106	6	0.15584692807566825
1106	7	0.12357151528792798
1106	8	0.118922029017547
1106	9	0.14608603558906452
1106	10-11	0.14492625328455944
1106	12-13	0.12597227610152117
1106	14-15	0.18064249967934387
1106	16-17	0.23093759005440972
1106	18-19	0.18191834968002496
1106	20-21	0.16852739717976561
1106	22-23	0.14186202572653173
1106	24-25	0.143132181352442
1106	26-27	0.1253340084810617
1106	28-29	0.1248079485714726
1106	30-31	0.16376463467803148
1106	32-33	0.17909810545116045
1106	34-35	0.1783283229973236
1106	36-37	0.14324682112890486
1106	38-39	0.14650350162458636
1106	40-41	0.14468901126897293
1106	42-43	0.14576933745041032
1106	44-45	0.21028626011266027
1106	46-47	0.15818448135696883
1106	48-49	0.15802757206376583
1106	50-51	0.1616101672287229
1106	52-53	0.15740830417670537
1106	54-55	0.2152974738924911
1106	56-57	0.22145251326783466
1106	58-59	0.2392065969195869
1106	60-61	0.22391645271801508
1106	62-63	0.1770724471799241
1106	64-65	0.16797274405126217
1106	66-67	0.1810523787880527
1106	68-69	0.21332122546644428
1106	70-71	0.24224864224171228
1106	72-73	0.26532363977213436
1106	74-75	0.27670240117703315
1106	76-77	0.23232735740731414
1106	78-79	0.2154579631413327
1106	80-81	0.096281565789063
1106	82-83	0.1960107431070952
1106	84-85	0.20452044077293152
1106	86-87	0.1651288356980558
1106	88-89	0.13315604958665972
1106	90-91	0.1730595186971371
1106	92-93	0.21207314285463497
1106	94-95	0.18808919376700928
1106	96-97	0.17090000727096566
1106	98-99	0.17709411029643363
1106	100	0.15611975504570097
1107	1	0.1579386052965468
1107	2	0.12666469936029046
1107	3	0.2291083373561591
1107	4	0.13134279968288354
1107	5	0.14603540922661296
1107	6	0.11643701224950576
1107	7	0.11953760176204042
1107	8	0.1237994883403033
1107	9	0.12715639518530253
1107	10-11	0.1336763947074644
1107	12-13	0.1269431259864433
1107	14-15	0.17211602250224445
1107	16-17	0.18215910322736306
1107	18-19	0.1918037508621353
1107	20-21	0.20275366795564054
1107	22-23	0.22382503959443767
1107	24-25	0.2022633807592129
1107	26-27	0.19443214349892912
1107	28-29	0.18607394572106983
1107	30-31	0.15634139699157856
1107	32-33	0.16496846413431143
1107	34-35	0.20324660833587416
1107	36-37	0.20009665297236268
1107	38-39	0.19070345499097385
1107	40-41	0.18096179115553213
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2216	56-57	-0.639663501195507
2216	58-59	-0.5452959578167338
2216	60-61	-0.6209355734513053
2216	62-63	-0.6607572781123707
2216	64-65	-0.644541943554195
2216	66-67	-0.6458673698240887
2216	68-69	-0.6467295402144586
2216	70-71	-0.6230905953743147
2216	72-73	-0.6147632828329961
2216	74-75	-0.654879185323594
2216	76-77	-0.5283542451935546
2216	78-79	-0.5905552206616704
2216	80-81	-0.594685252418131
2216	82-83	-0.5981612222333581
2216	84-85	-0.6992114341561759
2216	86-87	-0.6806316217872208
2216	88-89	-0.6970889980455439
2216	90-91	-0.681683323288695
2216	92-93	-0.6523259263633818
2216	94-95	-0.6785659383472655
2216	96-97	-0.7184227833880783
2216	98-99	-0.7674966803531298
2216	100	-0.7544997851254678
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	438.0
3	43.0
4	60.0
5	146.0
6	545.0
7	1299.0
8	2700.0
9	4358.0
10	5660.0
11	7059.0
12	10473.0
13	15094.0
14	17815.0
15	22135.0
16	27559.0
17	38844.0
18	40224.0
19	21203.0
20	14384.0
21	13577.0
22	13785.0
23	14681.0
24	16997.0
25	20969.0
26	26052.0
27	34216.0
28	44426.0
29	57712.0
30	75201.0
31	96837.0
32	127284.0
33	167832.0
34	230407.0
35	346040.0
36	568036.0
37	1093533.0
38	1649400.0
39	734056.0
40	2625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76586014282277	20.676546330292815	15.018185345647547	22.53940818123687
2	15.529256134176775	21.124322730985917	40.05446011246103	23.291961022376277
3	17.57577010283615	26.21963242120134	31.42986553025367	24.77473194570884
4	12.475050348643576	18.13365374332392	38.77867356374934	30.612622344283167
5	12.242503817254963	37.59757953498338	35.660711377415154	14.499205270346499
6	29.034692629296856	38.85274680162748	17.957473899062297	14.155086670013366
7	25.204427625116715	33.473485743762474	23.295807380154052	18.026279250966756
8	24.193159054982246	35.77901775884955	21.093444026956856	18.934379159211353
9	24.338673599696605	16.930247020645414	22.546702242480503	36.18437713717748
10-11	23.06651772514898	27.286259785520624	29.222730177103205	20.424492312227194
12-13	24.19404874988879	25.059793788491664	29.52315947736266	21.222997984256896
14-15	21.443804083789487	26.941121069503144	27.72171601477792	23.893358831929444
16-17	19.991759088592943	30.134883139922046	28.298409063744394	21.574948707740617
18-19	19.804123331485044	28.07006302454929	31.81309037772492	20.312723266240752
20-21	20.96120301130272	27.360913276314974	31.670397693623226	20.00748601875908
22-23	20.634756875139857	27.290977864570458	31.75690479635423	20.31736046393545
24-25	20.41737475297486	27.305590429399114	31.77403367000946	20.503001147616562
26-27	20.253554061547117	27.283788410780225	31.81959683340508	20.64306069426758
28-29	20.327461646510734	27.26115960497546	31.930386316312603	20.4809924322012
30-31	20.590910193836663	27.634480979850657	31.47452102510827	20.300087801204413
32-33	20.22050054774651	27.42464778416541	31.879853442984484	20.474998225103597
34-35	20.46535537020744	27.162915718931902	31.713902875871387	20.657826034989274
36-37	20.827892564397285	27.508971090307625	31.162831242849865	20.500305102445225
38-39	20.643626863753557	27.616129899051085	31.239884213846707	20.50035902334865
40-41	20.545931173561502	27.442540537285854	31.25721079748118	20.75431749167147
42-43	20.528011459989344	27.551775300811236	31.039442242174953	20.880770997024463
44-45	20.821251306458556	27.77451356604996	30.653539323166846	20.750695804324636
46-47	20.91664637143774	27.95095174887957	30.225928585358137	20.906473294324552
48-49	20.96332390017084	27.84220227348502	30.046911185981283	21.147562640362853
50-51	21.031246264854087	27.901452359533803	29.80015115826594	21.267150217346177
52-53	21.034193940908082	28.094830692856647	29.617539031993967	21.2534363342413
54-55	21.167711084609987	28.409575992975906	29.45681879251326	20.965894129900846
56-57	20.975563945248716	28.083318579975035	29.545824230436374	21.39529324433988
58-59	20.887654899028615	27.709799495120606	29.909601605405033	21.492944000445746
60-61	21.122965721583007	28.110395860312508	29.544206603333574	21.22243181477091
62-63	20.939598702663066	28.1029278151879	29.622913148702168	21.334560333446866
64-65	20.99376225015525	27.86815620166777	29.78569136933033	21.35239017884665
66-67	20.891968571302755	27.920513398895157	29.653396432772766	21.53412159702932
68-69	21.312947037989975	28.039004584175476	29.479744163286874	21.168304214547682
70-71	21.32045103038353	27.861523930546284	29.520229774943136	21.29779526412705
72-73	21.0492019256952	28.018263009990648	29.692381245950315	21.24015381836384
74-75	21.15601024856638	28.0619569154008	29.434989813442662	21.347043022590164
76-77	21.260230368073074	28.09004970608614	29.213231111282862	21.436488814557926
78-79	21.32712823559121	28.14984798798642	29.374859019304584	21.148164757117787
80-81	21.140913571666147	28.024195450843287	29.44529355110605	21.38959742638452
82-83	21.06143776846598	28.11396720923714	29.532904581606694	21.29169044069019
84-85	21.120714950034504	28.006487421701014	29.36910839470822	21.50368923355626
86-87	21.2502047879996	28.145954822531593	29.236617738923005	21.367222650545802
88-89	21.002183089807204	28.075530665527854	29.3256578297003	21.596628414964645
90-91	20.915526253519825	28.018364158795727	29.373047093879777	21.693062493804664
92-93	21.05969192222611	28.17885783587069	29.224871791530532	21.536578450372662
94-95	20.89333590117558	27.983761842756927	29.371564945574296	21.751337310493195
96-97	20.904258832632582	28.086610058374212	29.346712984993275	21.66241812399993
98-99	20.82477291622275	28.135516663954114	29.28929358394023	21.75041683588291
100	20.95613008539822	28.065087633245433	29.233842449061314	21.744939832295028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2558.0
1	1862.0
2	2825.5
3	3984.5
4	2157.0
5	1058.0
6	2490.5
7	6406.0
8	10687.0
9	11991.0
10	8721.0
11	4776.5
12	3969.5
13	4404.0
14	5102.5
15	5775.0
16	6461.0
17	7283.0
18	8311.5
19	9518.5
20	11178.0
21	13626.0
22	17240.0
23	23126.0
24	30505.0
25	39280.5
26	50974.5
27	64410.5
28	80507.0
29	98659.5
30	115218.0
31	129998.5
32	142439.0
33	153950.5
34	165233.5
35	173997.5
36	181148.5
37	188486.0
38	194321.0
39	196279.5
40	197041.0
41	195774.0
42	191795.0
43	188823.5
44	184409.5
45	179567.5
46	175515.0
47	169814.5
48	165664.5
49	163463.5
50	159153.0
51	151039.0
52	139092.0
53	129445.5
54	123786.5
55	117099.5
56	107922.0
57	97459.0
58	88583.5
59	81293.5
60	72077.0
61	60766.0
62	51074.0
63	42497.0
64	34356.5
65	27269.0
66	21379.0
67	16710.0
68	13001.0
69	10096.5
70	7456.0
71	5426.5
72	4248.0
73	3289.5
74	2462.0
75	1803.5
76	1182.5
77	866.5
78	628.5
79	343.0
80	175.5
81	83.5
82	52.5
83	30.0
84	15.5
85	10.0
86	6.0
87	4.0
88	3.5
89	2.0
90	1.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13135132074759537
2	0.0
3	0.0
4	0.0
5	0.014504723021799322
6	2.8757815160940415E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.6061769989602253E-4
82-83	6.200903894077777E-4
84-85	2.785913343716103E-4
86-87	4.4035404465190013E-4
88-89	5.571826687432206E-4
90-91	7.459058307368921E-4
92-93	2.24670430944847E-4
94-95	3.5048587227396133E-4
96-97	5.841431204566022E-4
98-99	0.0011592994236754106
100	1.0784180685352655E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5563705.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.68320866833396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21592699530747	44.8912786604311
2	15.273226325085146	18.231103075974968
3	4.480541305187615	8.022392449938057
4	1.754366095901819	4.188247911294347
5	0.9308201006445551	2.7777165149724303
6	0.5391604131492441	1.9307294066214897
7	0.3552339495356775	1.4841051355349981
8	0.24684698601689542	1.1786096140476547
9	0.1875515470229156	1.007431030533377
>10	0.9419692443176491	9.867009933047061
>50	0.05033154039407583	2.0505013471487827
>100	0.02263973670449354	2.3583446002882718
>500	8.736350817905468E-4	0.34643368411857905
>1k	3.615004592691385E-4	0.41926604643340154
>5k	6.025007653423499E-5	0.19971727961481206
>10k+	9.037511480135248E-5	1.0471133100006338
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23332	0.41936083958441356	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21839	0.3925262033123611	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13068	0.23487945532698085	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5997	0.10778788595009979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.594726895117552E-5	0.0
4	0.0	0.0	0.0	3.594726895117552E-5	0.0
5	0.0	0.0	0.0	3.594726895117552E-5	0.0
6	0.0	0.0	0.0	3.594726895117552E-5	0.0
7	0.0	0.0	0.0	3.594726895117552E-5	0.0
8	0.0	0.0	0.0	5.392090342676328E-5	0.0
9	0.0	0.0	0.0	1.0784180685352656E-4	0.0
10-11	0.0	0.0	0.0	1.1682862409132045E-4	0.0
12-13	0.0	0.0	0.0	1.437890758047021E-4	0.0
14-15	0.0	8.98681723779388E-6	0.0	1.8872316199367148E-4	0.0
16-17	0.0	1.797363447558776E-5	0.0	1.9770997923146535E-4	0.0
18-19	0.0	1.797363447558776E-5	0.0	2.24670430944847E-4	0.0
20-21	0.0	1.797363447558776E-5	0.0	2.6061769989602253E-4	0.0
22-23	8.98681723779388E-6	3.594726895117552E-5	0.0	3.235254205605797E-4	0.0
24-25	1.797363447558776E-5	5.392090342676328E-5	0.0	5.481958515054267E-4	0.0
26-27	1.797363447558776E-5	5.392090342676328E-5	0.0	7.728662824502738E-4	0.0
28-29	1.797363447558776E-5	5.392090342676328E-5	0.0	0.0013929566718580513	0.0
30-31	5.392090342676328E-5	5.392090342676328E-5	0.0	0.0032622146573191785	0.0
32-33	5.392090342676328E-5	8.98681723779388E-5	0.0	0.007216414241948486	0.0
34-35	5.392090342676328E-5	8.98681723779388E-5	0.0	0.013749830373824636	0.0
36-37	5.392090342676328E-5	8.98681723779388E-5	0.0	0.023671276604349083	0.0
38-39	5.392090342676328E-5	1.1682862409132045E-4	0.0	0.03515642903424966	0.0
40-41	7.189453790235104E-5	1.437890758047021E-4	0.0	0.04775594680163668	0.0
42-43	7.189453790235104E-5	1.797363447558776E-4	0.0	0.05996903142779856	0.0
44-45	7.189453790235104E-5	1.797363447558776E-4	0.0	0.07582177703526696	0.0
46-47	7.189453790235104E-5	1.797363447558776E-4	0.0	0.09453233052435384	0.0
48-49	7.189453790235104E-5	1.797363447558776E-4	0.0	0.11424940754407359	0.0
50-51	7.189453790235104E-5	2.696045171338164E-4	0.0	0.1347932717496704	0.0
52-53	8.088135514014493E-5	2.696045171338164E-4	0.0	0.15426770470396975	0.0
54-55	8.98681723779388E-5	2.785913343716103E-4	0.0	0.1776963372428984	0.0
56-57	8.98681723779388E-5	3.145386033227858E-4	0.0	0.20625644242460733	0.0
58-59	8.98681723779388E-5	3.235254205605797E-4	0.0	0.2361465965575098	0.0
60-61	8.98681723779388E-5	3.4149905503616745E-4	0.0	0.26787006140692216	0.0
62-63	8.98681723779388E-5	3.5048587227396133E-4	0.0	0.3003484189043093	0.0
64-65	8.98681723779388E-5	3.594726895117552E-4	0.0	0.33572951836950377	0.0
66-67	8.98681723779388E-5	3.684595067495491E-4	0.0	0.37766200760105	0.0
68-69	8.98681723779388E-5	3.7744632398734295E-4	0.0	0.4236385645896035	0.0
70-71	8.98681723779388E-5	3.7744632398734295E-4	0.0	0.47331769028012805	0.0
72-73	8.98681723779388E-5	4.0440677570072463E-4	0.0	0.5282001831513353	0.0
74-75	8.98681723779388E-5	4.133935929385185E-4	0.0	0.5861831279695815	0.0
76-77	8.98681723779388E-5	4.133935929385185E-4	0.0	0.6495761367649795	0.0
78-79	8.98681723779388E-5	4.133935929385185E-4	0.0	0.7229624863288042	0.0
80-81	8.98681723779388E-5	4.223804101763124E-4	0.0	0.8060276380577331	0.0
82-83	8.98681723779388E-5	4.3136722741410626E-4	0.0	0.8936580929434612	0.0
84-85	9.885498961573268E-5	4.4035404465190013E-4	0.0	0.9845058284003196	0.0
86-87	1.0784180685352656E-4	4.49340861889694E-4	0.0	1.084160644750216	0.0
88	1.0784180685352656E-4	4.49340861889694E-4	0.0	1.1667944292517307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11245	0.0	59.518883	1
GTATCAA	20905	0.0	41.404373	1
GTGGTAT	4260	0.0	37.012623	1
TGGTATC	4655	0.0	32.71789	2
ATCAACG	26085	0.0	32.707382	3
TCAACGC	26360	0.0	32.277	4
TATCAAC	27195	0.0	31.45881	2
CAACGCA	27510	0.0	30.910639	5
AACGCAG	28550	0.0	29.878332	6
ACGCAGA	31920	0.0	26.620815	7
CGCAGAG	32245	0.0	26.337925	8
GCAGAGT	34805	0.0	24.144138	9
GTACATG	15250	0.0	22.221703	1
TACATGG	15245	0.0	21.5531	2
CAGAGTA	34270	0.0	21.517641	10-11
GAGTACT	27315	0.0	21.481922	12-13
AGAGTAC	33060	0.0	21.281624	10-11
ACATGGG	15730	0.0	20.081701	3
AGTACTT	28465	0.0	19.763725	12-13
GTACTTT	30265	0.0	19.294857	14-15
>>END_MODULE