FastQCFastQC Report
Wed 25 May 2016
SRR2049461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042998
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65100.16101912491670783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.044.089261
GTATCAA71600.032.1843381
TCAACGC74150.029.9192354
ATCAACG75250.029.5454353
CAACGCA81550.027.4924895
TATCAAC85300.026.5055732
AACGCAG86550.026.1205026
GTACCTG30800.024.2777231
GTGGTAT26350.024.2727971
TACCTGG29850.023.9353832
TATAACG3350.023.8531232
GTACATG105000.023.111171
CGCAGAG97550.022.9803688
ACGCAGA98300.022.7600427
TACATGG106300.021.7556932
ACATGGG107350.020.6669183
TGGTATC28200.019.335262
GCAGAGT113650.019.1873259
CGCTCTG12600.019.0247655
GTCTCGC12550.018.3617421
Produced by FastQC (version 0.11.5)