FastQCFastQC Report
Wed 25 May 2016
SRR2049461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042998
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192290.47561240445827574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177750.4396489931481539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112200.2775168328057545No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99100.24511513485784558No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.14152863790682063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47200.11674504909475593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84100.058.0360261
GTATCAA152100.040.723171
GTGGTAT31400.035.824751
TATAACG4200.033.576332
TCAACGC190550.032.19314
ATCAACG190600.032.1599923
TATCAAC194800.031.5389962
CAACGCA197400.031.123595
AACGCAG205850.029.9549776
TGGTATC36450.029.532432
ACGCAGA230450.026.7369587
CGCAGAG232850.026.4210138
GTACCTG30800.024.9087411
ATAACGC5400.024.3739263
GCAGAGT253700.023.990289
GCGGTAT2653.6379788E-1123.0893841
TATACCG2504.3655746E-1022.5632925
CGGTATC3150.022.384222
TAACGCA6100.022.3475234
TACCTGG32500.022.274022
Produced by FastQC (version 0.11.5)