##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049461_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4042998 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00245857158475 33.0 31.0 34.0 30.0 34.0 2 32.15674432685844 33.0 31.0 34.0 30.0 34.0 3 32.1144333981862 34.0 31.0 34.0 30.0 34.0 4 35.84129598876873 37.0 35.0 37.0 35.0 37.0 5 35.88880083541966 37.0 35.0 37.0 35.0 37.0 6 35.94860398150086 37.0 35.0 37.0 35.0 37.0 7 35.94202569479381 37.0 35.0 37.0 35.0 37.0 8 35.954420704635524 37.0 35.0 37.0 35.0 37.0 9 37.67743046125672 39.0 37.0 39.0 35.0 39.0 10-11 37.647810857190635 39.0 37.0 39.0 35.0 39.0 12-13 37.624456034853345 39.0 37.0 39.0 35.0 39.0 14-15 38.92620686431208 40.0 38.0 41.0 35.5 41.0 16-17 38.93211535103406 40.0 38.0 41.0 35.5 41.0 18-19 39.01483144438855 40.0 38.5 41.0 36.0 41.0 20-21 39.02903006630228 40.0 39.0 41.0 35.5 41.0 22-23 38.97532363360061 40.0 39.0 41.0 35.0 41.0 24-25 38.938815329614314 40.0 38.0 41.0 35.0 41.0 26-27 38.826838400612615 40.0 38.0 41.0 35.0 41.0 28-29 38.73450741256859 40.0 38.0 41.0 35.0 41.0 30-31 38.58691569968622 40.0 38.0 41.0 35.0 41.0 32-33 38.403947763516086 40.0 38.0 41.0 34.0 41.0 34-35 38.249287162645146 40.0 38.0 41.0 34.0 41.0 36-37 38.08731466105103 40.0 38.0 41.0 34.0 41.0 38-39 37.9623956034606 40.0 38.0 41.0 33.0 41.0 40-41 37.85535869668993 40.0 38.0 41.0 33.0 41.0 42-43 37.69594197177441 40.0 37.0 41.0 33.0 41.0 44-45 37.49192913278711 40.0 37.0 41.0 33.0 41.0 46-47 37.20347821097117 40.0 37.0 41.0 32.5 41.0 48-49 36.91872763726324 40.0 37.0 41.0 31.0 41.0 50-51 36.75056554566686 40.0 36.5 41.0 31.0 41.0 52-53 36.628579336423115 40.0 36.0 41.0 31.0 41.0 54-55 36.48250939030888 40.0 36.0 41.0 31.0 41.0 56-57 36.328968997758594 39.0 35.0 41.0 30.5 41.0 58-59 36.15087590941178 39.0 35.0 41.0 30.0 41.0 60-61 35.951309523279505 39.0 35.0 41.0 30.0 41.0 62-63 35.71505526344559 39.0 35.0 41.0 29.5 41.0 64-65 35.4826319478763 38.0 35.0 40.0 29.0 41.0 66-67 35.21232572956999 37.5 35.0 40.0 29.0 41.0 68-69 34.9146406948507 37.0 35.0 40.0 29.0 41.0 70-71 34.618828156729236 36.5 34.5 39.5 28.5 41.0 72-73 34.283126160339435 36.0 34.0 39.0 28.0 41.0 74-75 33.96399045955501 36.0 34.0 39.0 28.0 40.5 76-77 33.651948133538525 35.0 34.0 38.5 28.0 40.0 78-79 33.34767763921724 35.0 34.0 37.0 27.0 39.0 80-81 33.047309076086606 35.0 34.0 37.0 27.0 39.0 82-83 32.78182366155016 35.0 34.0 36.0 27.0 39.0 84-85 32.50697415630678 35.0 34.0 36.0 26.0 37.0 86-87 32.26642320871789 35.0 33.0 36.0 26.0 37.0 88-89 32.05656792311052 35.0 33.0 35.0 26.0 37.0 90-91 31.87279303625676 35.0 33.0 35.0 26.0 36.0 92-93 31.709351946253747 35.0 33.0 35.0 25.0 36.0 94-95 31.544886492647287 35.0 33.0 35.0 25.0 36.0 96-97 31.399269922963107 35.0 33.0 35.0 25.0 36.0 98-99 31.26428815943021 35.0 33.0 35.0 24.5 35.0 100 31.158162333990767 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33910865825458814 1101 2 0.3371801299929231 1101 3 0.419823945389858 1101 4 0.2609175995041255 1101 5 0.2838111479962535 1101 6 0.21909259930919234 1101 7 0.2888895440561541 1101 8 0.27074650579237414 1101 9 0.31021311728059686 1101 10-11 0.3212236020470556 1101 12-13 0.31208530310350824 1101 14-15 0.45840931631978776 1101 16-17 0.436867535787556 1101 18-19 0.3966655225222837 1101 20-21 0.4435193123337555 1101 22-23 0.4168406647317582 1101 24-25 0.42960564426874726 1101 26-27 0.42036659098350526 1101 28-29 0.46699264464223234 1101 30-31 0.4031736543091853 1101 32-33 0.43987967829117025 1101 34-35 0.4390500910003112 1101 36-37 0.48209465660697504 1101 38-39 0.4878577279879863 1101 40-41 0.476370755327693 1101 42-43 0.4526684446888325 1101 44-45 0.4540095158506716 1101 46-47 0.47214730749715983 1101 48-49 0.4833561756824878 1101 50-51 0.42102872621484977 1101 52-53 0.40769219878616525 1101 54-55 0.3970540416830133 1101 56-57 0.4497353729329774 1101 58-59 0.41302241494829417 1101 60-61 0.3506771047329664 1101 62-63 0.40172187733264053 1101 64-65 0.4273856099470663 1101 66-67 0.4891196852325521 1101 68-69 0.4266033271682659 1101 70-71 0.484325064093575 1101 72-73 0.49504089766129766 1101 74-75 0.4238453036814249 1101 76-77 0.4636411480936573 1101 78-79 0.4189347557649157 1101 80-81 0.42400141950506054 1101 82-83 0.35805005965678305 1101 84-85 0.39659136952484175 1101 86-87 0.32966406784046853 1101 88-89 0.3751822975979877 1101 90-91 0.3942091180532401 1101 92-93 0.37542360658192564 1101 94-95 0.41539670658657357 1101 96-97 0.41014253242596155 1101 98-99 0.4664203619750822 1101 100 0.469550594120534 1102 1 0.21028833999990582 1102 2 0.19978548879054614 1102 3 0.32273129300411796 1102 4 0.19414380534696107 1102 5 0.12667059168246197 1102 6 0.12294945572978833 1102 7 0.16424909861564885 1102 8 0.14166375964284583 1102 9 0.18725704544270627 1102 10-11 0.13958630207329747 1102 12-13 0.1769828378485272 1102 14-15 0.24582373131399038 1102 16-17 0.24185166506880762 1102 18-19 0.22442400816327535 1102 20-21 0.24032199356656037 1102 22-23 0.21316222259977735 1102 24-25 0.177387394959581 1102 26-27 0.15923274575460766 1102 28-29 0.19138186622507902 1102 30-31 0.20697177289791568 1102 32-33 0.21104095340396611 1102 34-35 0.24121540456829393 1102 36-37 0.20985170839797718 1102 38-39 0.17010036275070917 1102 40-41 0.17446818643536943 1102 42-43 0.11887473435520945 1102 44-45 0.1219414606614535 1102 46-47 0.12516617863038704 1102 48-49 0.17468004938498183 1102 50-51 0.07247776501850467 1102 52-53 -0.011090135781721244 1102 54-55 0.0493293448839367 1102 56-57 0.09919292747179753 1102 58-59 0.07572817659107045 1102 60-61 0.054482492607732524 1102 62-63 -0.00727841577297994 1102 64-65 -0.043089834704829855 1102 66-67 0.002478165404909305 1102 68-69 0.050496703414836475 1102 70-71 0.006117183187157593 1102 72-73 0.03554297524325278 1102 74-75 0.036305410139789274 1102 76-77 0.03393017557323219 1102 78-79 0.055067108789451424 1102 80-81 0.05943253651755498 1102 82-83 0.06549549249441355 1102 84-85 -0.07737583332391296 1102 86-87 -0.060698792910351074 1102 88-89 -0.05152989436184896 1102 90-91 0.01467876061443718 1102 92-93 -0.0067774965489100225 1102 94-95 -0.019892909438400608 1102 96-97 0.022028832122618525 1102 98-99 0.005765272591204962 1102 100 0.04153149790619892 1103 1 0.2614167989305827 1103 2 0.21016142664210236 1103 3 0.31344072574390935 1103 4 0.18126266968766203 1103 5 0.15754601260049128 1103 6 0.14248884707694742 1103 7 0.13949962472364064 1103 8 0.11929021854061972 1103 9 0.1663024457466662 1103 10-11 0.20739637044519554 1103 12-13 0.1603834505760986 1103 14-15 0.2689839984237281 1103 16-17 0.22888311892378965 1103 18-19 0.21378944536755995 1103 20-21 0.20624538110725865 1103 22-23 0.2028706265342919 1103 24-25 0.24634778279259706 1103 26-27 0.20164122574225019 1103 28-29 0.20611092851904544 1103 30-31 0.22932332692538182 1103 32-33 0.2100450812795316 1103 34-35 0.18335519933872746 1103 36-37 0.15776072176991107 1103 38-39 0.20316076108690595 1103 40-41 0.17456632155179364 1103 42-43 0.21380767852259197 1103 44-45 0.18525408079946715 1103 46-47 0.09337195107587348 1103 48-49 0.05978315769299769 1103 50-51 -0.04535296171285097 1103 52-53 0.009546268233783906 1103 54-55 -0.02227610386119494 1103 56-57 0.02314969613740203 1103 58-59 0.03108340546544497 1103 60-61 -0.10087824094435405 1103 62-63 -0.043837701151922204 1103 64-65 -0.07201786515645381 1103 66-67 -0.03094677546725677 1103 68-69 -0.017052620395610063 1103 70-71 -0.06644478240038154 1103 72-73 7.12158665436391E-4 1103 74-75 -0.040687789368718086 1103 76-77 -0.10280276024222701 1103 78-79 -0.08144393156932495 1103 80-81 -0.13089909816925172 1103 82-83 -0.1554760641398616 1103 84-85 -0.07912578302254758 1103 86-87 -0.1543798025315155 1103 88-89 -0.12451603649389398 1103 90-91 -0.10346709309780167 1103 92-93 -0.07431188813016831 1103 94-95 -0.13493162567162997 1103 96-97 -0.09004589988815681 1103 98-99 -0.045340092001946886 1103 100 -0.029318070008237385 1104 1 0.2530524048889049 1104 2 0.24023876471535743 1104 3 0.33105225039182073 1104 4 0.2170148034171291 1104 5 0.1746033650864689 1104 6 0.13223251109238987 1104 7 0.17767581474851823 1104 8 0.1722490797565186 1104 9 0.2497512593879918 1104 10-11 0.23530922537292298 1104 12-13 0.14920329232707275 1104 14-15 0.2896079896122359 1104 16-17 0.2588156571740896 1104 18-19 0.23195771760843797 1104 20-21 0.30144900741112224 1104 22-23 0.25060972531036896 1104 24-25 0.2604212105737531 1104 26-27 0.2888770137865748 1104 28-29 0.3240228878027551 1104 30-31 0.34874542807473574 1104 32-33 0.31243546305658043 1104 34-35 0.3090651093307457 1104 36-37 0.3082437332571857 1104 38-39 0.32197460602244377 1104 40-41 0.2729201545082489 1104 42-43 0.2888667982996509 1104 44-45 0.2660527758982454 1104 46-47 0.28749700503872333 1104 48-49 0.30252683528247815 1104 50-51 0.3127875092044121 1104 52-53 0.32247847101146476 1104 54-55 0.2914208807393024 1104 56-57 0.24705933741197583 1104 58-59 0.23984239190183132 1104 60-61 0.24922064348798756 1104 62-63 0.197946916216587 1104 64-65 0.24273907596349886 1104 66-67 0.24396773234180813 1104 68-69 0.3017320221818238 1104 70-71 0.2059269871910132 1104 72-73 0.3055486000087555 1104 74-75 0.2855374855842996 1104 76-77 0.28719939827198004 1104 78-79 0.25708366238664127 1104 80-81 0.23018845363932883 1104 82-83 0.19785289560658015 1104 84-85 0.21857201795928916 1104 86-87 0.24373014619498434 1104 88-89 0.20347207555880686 1104 90-91 0.12391380503317606 1104 92-93 0.17548235007914315 1104 94-95 0.16232832173382405 1104 96-97 0.11906273918621224 1104 98-99 0.16137769868786478 1104 100 0.1307130945253263 1105 1 0.11846397436764278 1105 2 0.15080557283533835 1105 3 0.23976112565649288 1105 4 0.15429494616951445 1105 5 0.15279579304106505 1105 6 0.13697541972162952 1105 7 0.12500650741637997 1105 8 0.09074393332479502 1105 9 0.13689939823094477 1105 10-11 0.06819584084483665 1105 12-13 0.08305486888611568 1105 14-15 0.16775766913702483 1105 16-17 0.18073455484601197 1105 18-19 0.17380937004461572 1105 20-21 0.18896437517505404 1105 22-23 0.10374728622204543 1105 24-25 0.15600539585116735 1105 26-27 0.13801152692724372 1105 28-29 0.1778456627613707 1105 30-31 0.16649625693854375 1105 32-33 0.10976203886095703 1105 34-35 0.12890810942365505 1105 36-37 0.14360566428580057 1105 38-39 0.19172984821088335 1105 40-41 0.18554577610471767 1105 42-43 0.17883155301504416 1105 44-45 0.2111856155133438 1105 46-47 0.22782374422561702 1105 48-49 0.30650783052512054 1105 50-51 0.28608715355674263 1105 52-53 0.2346706180092184 1105 54-55 0.2962162044991956 1105 56-57 0.2494142303071598 1105 58-59 0.2524212597890454 1105 60-61 0.2753296918606125 1105 62-63 0.24661937162790792 1105 64-65 0.15818874257977455 1105 66-67 0.16038301193087534 1105 68-69 0.1835460038489174 1105 70-71 0.1872846430242774 1105 72-73 0.15975748508108012 1105 74-75 0.15580986103097416 1105 76-77 0.11364638182085685 1105 78-79 0.11170984237309511 1105 80-81 0.16881779415538745 1105 82-83 0.14702302167186332 1105 84-85 0.21099328925848226 1105 86-87 0.2143015903378327 1105 88-89 0.20402377050263 1105 90-91 0.2695971532776582 1105 92-93 0.224716337447596 1105 94-95 0.21842593098134344 1105 96-97 0.21947333318559714 1105 98-99 0.2442236153524462 1105 100 0.23480895671928081 1106 1 0.1075725528699607 1106 2 0.09149129556827518 1106 3 0.14283010360609438 1106 4 0.06645186334553443 1106 5 0.08087440509771682 1106 6 0.06270026285353936 1106 7 0.0010241540536952698 1106 8 0.04217917548082539 1106 9 0.05044678647107048 1106 10-11 0.1133595191045913 1106 12-13 0.09075253452645171 1106 14-15 0.13088479590494728 1106 16-17 0.07612380944541997 1106 18-19 0.09407419937563333 1106 20-21 0.08278587985613939 1106 22-23 0.08264512180633687 1106 24-25 0.03802668511191598 1106 26-27 0.03031078627176953 1106 28-29 0.001082395481617482 1106 30-31 0.0017649596601785333 1106 32-33 -0.011506909861893178 1106 34-35 -0.04444727938520998 1106 36-37 -0.09775210441550541 1106 38-39 -0.016905922734906653 1106 40-41 -0.011496736402698104 1106 42-43 -0.021179816077612656 1106 44-45 0.030317795462103447 1106 46-47 -0.08049301505825213 1106 48-49 -0.05129037964680094 1106 50-51 -0.08103055294463246 1106 52-53 -0.041087909233439746 1106 54-55 -0.0015629292944296935 1106 56-57 0.04856467979538337 1106 58-59 -0.017912320109722657 1106 60-61 -0.06746134421636896 1106 62-63 0.008330891198816914 1106 64-65 0.050827776991170026 1106 66-67 0.025954860364784338 1106 68-69 -0.018444602967235824 1106 70-71 0.012441934280118971 1106 72-73 -0.018428555027270477 1106 74-75 0.010877627029657333 1106 76-77 -0.02092054038239155 1106 78-79 -0.07110039985347072 1106 80-81 -0.006633965063592484 1106 82-83 -0.03184964956839309 1106 84-85 -0.0705174628563725 1106 86-87 -0.0018181750131205376 1106 88-89 -0.03696601198377181 1106 90-91 -0.006577403765000156 1106 92-93 -0.004191089927033431 1106 94-95 -0.05105014286190013 1106 96-97 -0.06464678085514919 1106 98-99 -0.045925249233480514 1106 100 -0.07482881778224026 1107 1 0.08843925432414323 1107 2 0.09646946585426974 1107 3 0.22403587395815805 1107 4 0.07355584629746659 1107 5 0.1254494121263292 1107 6 0.07420065503357165 1107 7 0.08602411576554658 1107 8 0.07292406592749501 1107 9 0.05629289437145246 1107 10-11 0.08107897309372447 1107 12-13 0.08471307147944174 1107 14-15 0.12270579115612179 1107 16-17 0.1544754445134231 1107 18-19 0.16799529406659275 1107 20-21 0.1064973469376298 1107 22-23 0.14878745963689255 1107 24-25 0.14173100871896338 1107 26-27 0.1300158034412675 1107 28-29 0.20943820942747493 1107 30-31 0.2024577836955075 1107 32-33 0.14150850057063735 1107 34-35 0.12211327441455921 1107 36-37 0.15117793329284268 1107 38-39 0.10912796665173374 1107 40-41 0.16508234886062922 1107 42-43 0.14644903174450263 1107 44-45 0.1003696266748193 1107 46-47 0.10490623733831939 1107 48-49 0.154589585915744 1107 50-51 0.17444156179729475 1107 52-53 0.18203192391774792 1107 54-55 0.24580238120604037 1107 56-57 0.180938840327741 1107 58-59 0.22734656793682717 1107 60-61 0.192469808224061 1107 62-63 0.17533438417185465 1107 64-65 0.21451838797155176 1107 66-67 0.15702547355494545 1107 68-69 0.15521610496968208 1107 70-71 0.16132124019257787 1107 72-73 0.1947349922492947 1107 74-75 0.1871509267350575 1107 76-77 0.20176395652774914 1107 78-79 0.18381164133145234 1107 80-81 0.2192317846566283 1107 82-83 0.21388075701892006 1107 84-85 0.1900959570300529 1107 86-87 0.14730114288899898 1107 88-89 0.1657723290017188 1107 90-91 0.17907504268355012 1107 92-93 0.15082014490949192 1107 94-95 0.15795771824182836 1107 96-97 0.16271070245395336 1107 98-99 0.16516846811903463 1107 100 0.1761426619234001 1108 1 0.015968441871045513 1108 2 0.06837148714000563 1108 3 0.14231884628439673 1108 4 0.10342975131795384 1108 5 0.06684874886362024 1108 6 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-0.12668821128059804 2214 96-97 -0.10365660928256304 2214 98-99 -0.12583929861693832 2214 100 -0.1062685176053364 2215 1 -0.40995698876482933 2215 2 -0.37809536304375513 2215 3 -0.2787481755719483 2215 4 -0.2240984768414691 2215 5 -0.24936081031827229 2215 6 -0.24479885456656802 2215 7 -0.2719300980913886 2215 8 -0.3160261575183725 2215 9 -0.43976834835656575 2215 10-11 -0.4150416334642486 2215 12-13 -0.3979902377100686 2215 14-15 -0.5466985265200961 2215 16-17 -0.557654477152596 2215 18-19 -0.4650917398772023 2215 20-21 -0.5092123346841646 2215 22-23 -0.5477266630770288 2215 24-25 -0.5620123630691296 2215 26-27 -0.42047685065645624 2215 28-29 -0.45092327169627566 2215 30-31 -0.45987573827443384 2215 32-33 -0.41977440261867116 2215 34-35 -0.49020979924470964 2215 36-37 -0.468919520195918 2215 38-39 -0.4926886771821586 2215 40-41 -0.5796407356411066 2215 42-43 -0.4633153467490416 2215 44-45 -0.47418864696277296 2215 46-47 -0.45585106949517495 2215 48-49 -0.47378972689934784 2215 50-51 -0.5113069631999565 2215 52-53 -0.4690578051508538 2215 54-55 -0.4220538093799959 2215 56-57 -0.35348464387892164 2215 58-59 -0.3898004997458244 2215 60-61 -0.4133074437499147 2215 62-63 -0.45100214906899083 2215 64-65 -0.46175183894833083 2215 66-67 -0.35650622970362633 2215 68-69 -0.34537269294767725 2215 70-71 -0.29619189038129434 2215 72-73 -0.3434439024674134 2215 74-75 -0.34329200004212623 2215 76-77 -0.35837692763581686 2215 78-79 -0.35527375324405597 2215 80-81 -0.44888751527474824 2215 82-83 -0.4353956957388121 2215 84-85 -0.39283728414240215 2215 86-87 -0.35380682059073365 2215 88-89 -0.3529280380652082 2215 90-91 -0.37618435063124167 2215 92-93 -0.3822851303602768 2215 94-95 -0.38154018061741723 2215 96-97 -0.33322890838485364 2215 98-99 -0.3384114785371892 2215 100 -0.32816569310175936 2216 1 -0.22414661250575563 2216 2 -0.28781218668296304 2216 3 -0.17697979177899015 2216 4 -0.18105179013260653 2216 5 -0.2031886676039676 2216 6 -0.20762078196808176 2216 7 -0.25093466148813093 2216 8 -0.2333779025916698 2216 9 -0.3435332715570709 2216 10-11 -0.3296522969018625 2216 12-13 -0.33984167458411463 2216 14-15 -0.4725206732308891 2216 16-17 -0.5216732472968388 2216 18-19 -0.5052829684728621 2216 20-21 -0.49460226858701617 2216 22-23 -0.44948793567392187 2216 24-25 -0.45342957608899326 2216 26-27 -0.47558276054394355 2216 28-29 -0.5077138523766394 2216 30-31 -0.4848403600740028 2216 32-33 -0.46713365995948664 2216 34-35 -0.4685717665201281 2216 36-37 -0.5234988033475361 2216 38-39 -0.4576275507630143 2216 40-41 -0.4987106879079093 2216 42-43 -0.6075380830633321 2216 44-45 -0.604396040729327 2216 46-47 -0.5503801988031682 2216 48-49 -0.5389316109055002 2216 50-51 -0.5562532210468092 2216 52-53 -0.5334228052091632 2216 54-55 -0.5588190912692284 2216 56-57 -0.47659076880898255 2216 58-59 -0.4449159955256192 2216 60-61 -0.48745062284709206 2216 62-63 -0.4658176149533091 2216 64-65 -0.4916137139204153 2216 66-67 -0.4985685562082267 2216 68-69 -0.4812889833609617 2216 70-71 -0.5046109491832027 2216 72-73 -0.42923840653686796 2216 74-75 -0.3873539095461851 2216 76-77 -0.3059144484396228 2216 78-79 -0.31194606051143836 2216 80-81 -0.33908174479514486 2216 82-83 -0.38658083345281824 2216 84-85 -0.454831582100951 2216 86-87 -0.3527134599731596 2216 88-89 -0.35140200383418474 2216 90-91 -0.3311335582022181 2216 92-93 -0.37823058236638474 2216 94-95 -0.4227863409271002 2216 96-97 -0.4179845804881701 2216 98-99 -0.5306968548175917 2216 100 -0.5443900291507191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 320.0 3 40.0 4 52.0 5 132.0 6 474.0 7 1082.0 8 2189.0 9 3533.0 10 4699.0 11 6299.0 12 9457.0 13 13950.0 14 14495.0 15 16828.0 16 21379.0 17 29774.0 18 30142.0 19 16206.0 20 11401.0 21 10698.0 22 10886.0 23 11863.0 24 13452.0 25 16447.0 26 20326.0 27 26381.0 28 33729.0 29 43573.0 30 56285.0 31 72607.0 32 95139.0 33 125147.0 34 174533.0 35 264789.0 36 421540.0 37 814062.0 38 1169212.0 39 478340.0 40 1537.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34646121718318 19.88735710981159 13.9966010487117 22.769580624293535 2 16.239854682094823 20.97448477590145 39.34661852417439 23.439042017829344 3 17.781235607833594 26.072731176221208 31.061677497738067 25.084355718207128 4 12.66048115779429 17.928032613422022 38.36971969810522 31.041766530678473 5 12.160092894666274 36.76688861248735 35.27310814193846 15.79991035090792 6 28.88063426388318 38.20052802557318 18.312974618264818 14.605863092278826 7 25.14023009657685 32.94817360780292 23.47156738638011 18.440028909240123 8 24.3740165095308 34.58554765547744 21.712229390170364 19.32820644482139 9 24.276786681566502 16.668348586865488 22.913194614491523 36.14167011707649 10-11 23.015952518403424 26.7005944598538 29.35959899065001 20.923854031092766 12-13 24.390538902072176 24.597489288889086 29.53204527927048 21.479926529768257 14-15 21.718412920313092 26.402597775215327 27.938722700332775 23.94026660413881 16-17 20.181682009241655 29.431191902642546 28.313704335248246 22.07342175286755 18-19 19.894778577679237 27.230659030748967 32.03668416358356 20.83787822798824 20-21 21.175016658430206 26.81438378154033 31.705840566826893 20.30475899320257 22-23 20.843925720467833 26.864012794465893 31.783283098334454 20.508778386731827 24-25 20.56929041270859 26.683713422564146 31.956421942330916 20.790574222396348 26-27 20.416656154665425 26.54381723661501 32.071818981854555 20.967707626865014 28-29 20.55912468915394 26.608299088943404 32.02571458111036 20.806861640792302 30-31 20.925795659557586 27.177010723230634 31.45262500748207 20.44456860972971 32-33 20.264640249636532 26.825959349967526 32.07379771150023 20.835602688895715 34-35 20.47223372358829 26.338561137057205 31.969469190932077 21.219735948422432 36-37 21.230705530895637 26.929756086943403 31.13834090444764 20.701197477713322 38-39 20.963379155765104 27.109461839951443 31.252662009726446 20.67449699455701 40-41 20.66143737889556 26.848220058481353 31.3714476237683 21.11889493885478 42-43 20.59814276435457 27.055096242936553 31.08141532595366 21.265345666755213 44-45 21.069772975400927 27.241900688548448 30.71360658600375 20.974719750046873 46-47 21.092973580496455 27.675737163362435 30.088575359176534 21.14271389696458 48-49 21.084007461789493 27.34744118102458 30.19678713667432 21.37176422051161 50-51 21.13739606104183 27.231277383763235 30.01830572263454 21.61302083256039 52-53 21.193010731145552 27.464000724215047 29.72573817746138 21.61725036717802 54-55 21.365976436298016 28.0228310773342 29.493237444094706 21.117955042273085 56-57 20.9984274046141 27.664891251492087 29.58470422196598 21.751977121927837 58-59 20.813836662793303 27.14883855990035 30.079411367505003 21.957913409801343 60-61 21.36073280273698 27.670530630982253 29.500200099035418 21.468536467245347 62-63 21.16243935811989 27.60106485335882 29.638822477775157 21.597673310746135 64-65 21.12647594680977 27.289019188236058 29.94018300281128 21.644321862142895 66-67 21.024187991188718 27.422162959269336 29.685297890327924 21.86835115921403 68-69 21.60991670042874 27.66473047970838 29.40071946609917 21.324633353763716 70-71 21.64422292566061 27.299976403648973 29.57802600941183 21.477774661278588 72-73 21.248118351777567 27.517723234095094 29.83819185663708 21.395966557490258 74-75 21.26800458471659 27.49784936821636 29.588698782438183 21.645447264628874 76-77 21.452817933622526 27.66994937914884 29.213148262749574 21.664084424479064 78-79 21.54656025058632 27.802746872494122 29.375169119549405 21.27552375737015 80-81 21.30134778863085 27.46736143248331 29.577370032547666 21.65392074633817 82-83 21.15900776544404 27.579773857150506 29.653630104857175 21.607588272548277 84-85 21.3124814029641 27.563435216027376 29.426205848503447 21.697877532505082 86-87 21.465575622721666 27.712703358761786 29.31664480984067 21.505076208675884 88-89 21.133006832960817 27.602967763972902 29.43338898952294 21.83063641354334 90-91 20.966814000598568 27.486414690190625 29.461324224518115 22.08544708469269 92-93 21.236186431800917 27.790282960100942 29.144119928058203 21.829410680039935 94-95 21.13014653267669 27.537324489381493 29.41735681200221 21.915172165939605 96-97 21.05222606689164 27.585477661665436 29.407157581991367 21.95513868945156 98-99 21.001422228818065 27.615305655523812 29.346257064767062 22.037015050891057 100 21.2233220405388 27.644459262956563 29.154578874897325 21.97763982160732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2635.0 1 1993.0 2 4920.5 3 7229.0 4 3672.0 5 1491.0 6 2814.5 7 6552.5 8 10295.5 9 11247.0 10 8071.5 11 4287.0 12 3488.0 13 3741.5 14 4142.0 15 4653.0 16 5278.0 17 5889.5 18 6628.0 19 7468.5 20 8355.0 21 9745.0 22 11940.0 23 15579.0 24 20512.5 25 26005.0 26 33277.5 27 42306.0 28 53171.5 29 65128.5 30 75110.0 31 84676.0 32 94425.0 33 102459.0 34 109315.5 35 115634.0 36 121045.0 37 126517.0 38 132576.5 39 135827.0 40 138030.0 41 138679.0 42 136289.0 43 134116.0 44 131370.0 45 129088.5 46 127399.5 47 125245.5 48 124051.0 49 123088.5 50 120709.0 51 115928.5 52 107800.5 53 100392.0 54 96121.5 55 92016.5 56 85771.5 57 78334.5 58 72519.0 59 67701.0 60 60315.0 61 51001.0 62 42741.5 63 34952.0 64 27854.5 65 22112.0 66 17340.5 67 13324.5 68 10094.0 69 7655.5 70 5715.0 71 4229.5 72 3196.5 73 2449.0 74 2036.5 75 1565.0 76 1017.0 77 749.5 78 522.5 79 315.0 80 179.5 81 87.5 82 43.0 83 21.5 84 12.5 85 7.0 86 6.0 87 5.0 88 2.0 89 3.0 90 3.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13064562485561457 2 0.0 3 0.0 4 0.0 5 0.014098448725426033 6 2.9680944685107436E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.236706028546143E-4 82-83 5.565177128457645E-4 84-85 3.7101180856384297E-4 86-87 4.946824114184572E-4 88-89 5.317835922748416E-4 90-91 6.925553759858402E-4 92-93 1.8550590428192149E-4 94-95 2.226070851383058E-4 96-97 6.554541951294559E-4 98-99 0.0011872377874042974 100 1.7313884399646005E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4042998.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.86311965102556 #Duplication Level Percentage of deduplicated Percentage of total 1 78.13570547963884 48.337184971041914 2 13.5135631806215 16.719823519089626 3 3.7562954193837124 6.9712845892180155 4 1.5467744019383876 3.827531596010319 5 0.8051619391084631 2.4904914688759305 6 0.4999215618588267 1.8556024438440104 7 0.32950941877137724 1.4269136419715518 8 0.23778929100572643 1.1768309888975832 9 0.18500635824848055 1.0300563428873628 >10 0.9246339489707943 9.983281010602191 >50 0.04572889679259798 1.9375100252297397 >100 0.01871190314459099 1.931870090099281 >500 6.390403345756762E-4 0.2648218651104807 >1k 3.5946011713819094E-4 0.46903458441941803 >5k 7.988002603069197E-5 0.38623761384626143 >10k+ 1.1982003904603793E-4 1.1915252488563275 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19229 0.47561240445827574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17775 0.4396489931481539 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11220 0.2775168328057545 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9910 0.24511513485784558 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5722 0.14152863790682063 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4720 0.11674504909475593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.4734120570922865E-5 0.0 5 0.0 0.0 0.0 2.4734120570922865E-5 0.0 6 0.0 0.0 0.0 2.4734120570922865E-5 0.0 7 0.0 0.0 0.0 2.4734120570922865E-5 0.0 8 0.0 0.0 0.0 4.946824114184573E-5 0.0 9 0.0 0.0 0.0 4.946824114184573E-5 0.0 10-11 0.0 0.0 0.0 4.946824114184573E-5 0.0 12-13 0.0 0.0 0.0 4.946824114184573E-5 0.0 14-15 0.0 1.2367060285461433E-5 0.0 4.946824114184573E-5 0.0 16-17 0.0 4.946824114184573E-5 0.0 4.946824114184573E-5 0.0 18-19 0.0 7.420236171276859E-5 0.0 8.656942199823002E-5 0.0 20-21 0.0 8.656942199823002E-5 0.0 1.4840472342553718E-4 0.0 22-23 0.0 9.893648228369146E-5 0.0 3.586447482783815E-4 0.0 24-25 0.0 9.893648228369146E-5 0.0 6.183530142730716E-4 0.0 26-27 0.0 9.893648228369146E-5 0.0 9.522636419805302E-4 0.0 28-29 0.0 1.113035425691529E-4 0.0 0.002040564947101136 0.0 30-31 0.0 1.2367060285461433E-4 0.0 0.004068762833916811 0.0 32-33 0.0 1.3603766314007577E-4 0.0 0.008941384586388615 0.0 34-35 0.0 1.4840472342553718E-4 0.0 0.017054176133651316 0.0 36-37 0.0 1.4840472342553718E-4 0.0 0.028654478681414138 0.0 38-39 0.0 1.4840472342553718E-4 0.0 0.04292606625083663 0.0 40-41 0.0 1.7313884399646005E-4 0.0 0.05812518334166873 0.0 42-43 0.0 1.7313884399646005E-4 0.0 0.07465994294333067 0.0 44-45 0.0 2.1024002485284436E-4 0.0 0.09412569583264696 0.0 46-47 0.0 2.4734120570922867E-4 0.0 0.11615143020105377 0.0 48-49 0.0 2.4734120570922867E-4 0.0 0.13861001167945172 0.0 50-51 0.0 2.7207532628015154E-4 0.0 0.16207032504097207 0.0 52-53 0.0 2.7207532628015154E-4 0.0 0.18460310888108278 0.0 54-55 0.0 3.091765071365358E-4 0.0 0.21100678259054295 0.0 56-57 0.0 3.462776879929201E-4 0.0 0.24207283802762208 0.0 58-59 0.0 3.7101180856384297E-4 0.0 0.27534023019551335 0.0 60-61 0.0 3.7101180856384297E-4 0.0 0.3097082907288107 0.0 62-63 0.0 3.7101180856384297E-4 0.0 0.3441010853826789 0.0 64-65 0.0 3.7101180856384297E-4 0.0 0.38429403131042855 0.0 66-67 0.0 3.8337886884930443E-4 0.0 0.4331068182571448 0.0 68-69 0.0 3.9574592913476584E-4 0.0 0.4856791915306413 0.0 70-71 0.0 3.9574592913476584E-4 0.0 0.5448926761774306 0.0 72-73 0.0 4.57581230562073E-4 0.0 0.6090777190589756 0.0 74-75 0.0 5.070494717039187E-4 0.0 0.6746231385719211 0.0 76-77 0.0 5.194165319893801E-4 0.0 0.7456842669721825 0.0 78-79 0.0 5.194165319893801E-4 0.0 0.828593039125916 0.0 80-81 0.0 5.194165319893801E-4 0.0 0.9191075533552082 0.0 82-83 0.0 5.194165319893801E-4 0.0 1.0160405718726548 0.0 84-85 0.0 5.194165319893801E-4 0.0 1.1147420800109227 0.0 86-87 0.0 5.194165319893801E-4 0.0 1.2228301869058555 0.0 88 0.0 5.194165319893801E-4 0.0 1.3174629322102065 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8410 0.0 58.036026 1 GTATCAA 15210 0.0 40.72317 1 GTGGTAT 3140 0.0 35.82475 1 TATAACG 420 0.0 33.57633 2 TCAACGC 19055 0.0 32.1931 4 ATCAACG 19060 0.0 32.159992 3 TATCAAC 19480 0.0 31.538996 2 CAACGCA 19740 0.0 31.12359 5 AACGCAG 20585 0.0 29.954977 6 TGGTATC 3645 0.0 29.53243 2 ACGCAGA 23045 0.0 26.736958 7 CGCAGAG 23285 0.0 26.421013 8 GTACCTG 3080 0.0 24.908741 1 ATAACGC 540 0.0 24.373926 3 GCAGAGT 25370 0.0 23.99028 9 GCGGTAT 265 3.6379788E-11 23.089384 1 TATACCG 250 4.3655746E-10 22.563292 5 CGGTATC 315 0.0 22.38422 2 TAACGCA 610 0.0 22.347523 4 TACCTGG 3250 0.0 22.27402 2 >>END_MODULE