FastQCFastQC Report
Wed 25 May 2016
SRR2049460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372547
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.045.914461
GTATCAA61450.035.2793271
ATCAACG65200.031.8599683
TCAACGC66700.031.2144014
CAACGCA71400.029.2267935
TATCAAC75550.028.1177852
AACGCAG75900.027.7424956
ACGCAGA83250.024.7854067
GTGGTAT20550.024.7145771
CGCAGAG83200.024.2332158
TGGTATC21350.022.8935182
GTACATG89950.021.6441441
TACATGG91400.020.6193662
GCAGAGT94750.020.3869679
ACATGGG92050.019.4523413
TATAACG2001.4093446E-518.79912
CAGAGTA90750.018.56660310-11
AGAGTAC83250.017.21890610-11
GTACACG3301.3722456E-817.100491
TACCTGG21750.017.0704482
Produced by FastQC (version 0.11.5)