##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3372547 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.298155073895188 31.0 31.0 34.0 27.0 34.0 2 30.509603276099636 31.0 31.0 34.0 27.0 34.0 3 30.62699971268006 31.0 31.0 34.0 27.0 34.0 4 34.219096427714724 37.0 35.0 37.0 30.0 37.0 5 34.232094022707464 37.0 35.0 37.0 30.0 37.0 6 34.22883891610702 37.0 35.0 37.0 30.0 37.0 7 34.09768344221741 37.0 35.0 37.0 30.0 37.0 8 34.05976343695136 37.0 35.0 37.0 30.0 37.0 9 35.56392157025536 39.0 35.0 39.0 30.0 39.0 10-11 35.48990451430329 39.0 35.0 39.0 30.0 39.0 12-13 35.39455061708554 39.0 35.0 39.0 29.5 39.0 14-15 36.531779542286586 40.0 37.0 41.0 29.0 41.0 16-17 36.510408898675095 40.0 37.0 41.0 29.0 41.0 18-19 36.524909067242056 40.0 37.0 41.0 29.0 41.0 20-21 36.56368287825195 40.0 37.0 41.0 29.0 41.0 22-23 36.51725594928699 40.0 37.0 41.0 29.0 41.0 24-25 36.50875866222176 40.0 37.0 41.0 29.0 41.0 26-27 36.494542551964436 40.0 37.0 41.0 29.5 41.0 28-29 36.46172699742954 40.0 36.0 41.0 30.0 41.0 30-31 36.39415551510476 40.0 36.0 41.0 30.0 41.0 32-33 36.329716976516565 40.0 36.0 41.0 30.0 41.0 34-35 36.24375138433949 40.0 36.0 41.0 29.0 41.0 36-37 36.11581780772811 40.0 36.0 41.0 29.0 41.0 38-39 35.986148006239794 40.0 36.0 41.0 28.0 41.0 40-41 35.84780864432727 40.0 35.0 41.0 28.0 41.0 42-43 35.740873737267414 40.0 35.0 41.0 27.0 41.0 44-45 35.57493787336396 39.0 35.0 41.0 27.0 41.0 46-47 35.4358318801784 39.0 35.0 41.0 27.0 41.0 48-49 35.31433646439916 39.0 35.0 41.0 26.0 41.0 50-51 35.18508400327705 39.0 35.0 41.0 26.0 41.0 52-53 35.05204908930847 39.0 35.0 41.0 26.0 41.0 54-55 34.880905588565554 39.0 34.0 40.0 26.0 41.0 56-57 34.693721836938074 38.5 34.0 40.0 25.0 41.0 58-59 34.49241982394908 38.0 34.0 40.0 25.0 41.0 60-61 34.269566591659064 38.0 34.0 40.0 24.0 41.0 62-63 33.98497470902555 37.5 33.5 40.0 23.0 41.0 64-65 33.72199408933367 37.0 33.0 40.0 23.0 41.0 66-67 33.482146431169085 36.5 33.0 40.0 22.5 41.0 68-69 33.17611363162618 36.0 33.0 39.0 22.0 41.0 70-71 32.85178857403618 36.0 33.0 39.0 20.5 41.0 72-73 32.507312129378775 35.0 32.0 39.0 20.0 40.0 74-75 32.212780281490524 35.0 32.0 38.0 19.5 40.0 76-77 31.891453996045126 35.0 32.0 37.0 18.5 39.5 78-79 31.55048854767628 35.0 32.0 37.0 17.5 39.0 80-81 31.223865968361597 35.0 31.5 36.0 14.0 39.0 82-83 30.935223289697667 35.0 31.0 36.0 11.0 37.5 84-85 30.65095534621163 35.0 31.0 35.5 7.5 37.0 86-87 30.377292147448202 35.0 31.0 35.0 4.5 37.0 88-89 30.139443571876093 35.0 31.0 35.0 2.0 36.0 90-91 29.919241451638776 34.0 31.0 35.0 2.0 36.0 92-93 29.683994767159657 34.0 31.0 35.0 2.0 36.0 94-95 29.445338789941253 34.0 31.0 35.0 2.0 35.5 96-97 29.20980626808166 34.0 30.0 35.0 2.0 35.0 98-99 28.98849623148321 34.0 30.0 35.0 2.0 35.0 100 28.814052702601327 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29538006339864253 1101 2 0.3069272746811791 1101 3 0.2981911226393059 1101 4 0.4075075630621754 1101 5 0.3305839399502233 1101 6 0.42538698904932915 1101 7 0.434628020246997 1101 8 0.47174448079993425 1101 9 0.5728740969619963 1101 10-11 0.5504878219465539 1101 12-13 0.5114281417357915 1101 14-15 0.6280371845099992 1101 16-17 0.6167655097772382 1101 18-19 0.6096059072254647 1101 20-21 0.582081145321709 1101 22-23 0.5353847205592857 1101 24-25 0.5700650880839859 1101 26-27 0.6042468395350653 1101 28-29 0.594730989431703 1101 30-31 0.6143073981528318 1101 32-33 0.6656784451426319 1101 34-35 0.5715869094238997 1101 36-37 0.6212464033820027 1101 38-39 0.6539516592654806 1101 40-41 0.6584324167742466 1101 42-43 0.5284570717253203 1101 44-45 0.5726267432517673 1101 46-47 0.5749022975328799 1101 48-49 0.4737930067403795 1101 50-51 0.5289795262460117 1101 52-53 0.5717772718408511 1101 54-55 0.44728169024792663 1101 56-57 0.47287436643000547 1101 58-59 0.4617342090019889 1101 60-61 0.4168887169211146 1101 62-63 0.43088401796259035 1101 64-65 0.46422399564844596 1101 66-67 0.43433068670898933 1101 68-69 0.4381615237796197 1101 70-71 0.48752678685606554 1101 72-73 0.435972032248074 1101 74-75 0.43088639434429865 1101 76-77 0.48215834808334535 1101 78-79 0.41740994215365035 1101 80-81 0.5144990601177284 1101 82-83 0.4541213859833313 1101 84-85 0.45461628997752257 1101 86-87 0.4863395128909005 1101 88-89 0.44683574551667604 1101 90-91 0.4515668827740953 1101 92-93 0.36731041882145377 1101 94-95 0.4408046858477448 1101 96-97 0.37991368330997943 1101 98-99 0.41354470529549303 1101 100 0.46063343782696364 1102 1 0.20630842096625912 1102 2 0.272309543134142 1102 3 0.3830885965193431 1102 4 0.3822167118656594 1102 5 0.34576474115370814 1102 6 0.3492700614028976 1102 7 0.4238626624787756 1102 8 0.3644375080353939 1102 9 0.4793536321529075 1102 10-11 0.4887828071898781 1102 12-13 0.5060878670903435 1102 14-15 0.5996553260693815 1102 16-17 0.538859479245346 1102 18-19 0.5067945585528904 1102 20-21 0.5040156748524396 1102 22-23 0.5872106994271036 1102 24-25 0.5006216871677225 1102 26-27 0.45098694824906715 1102 28-29 0.5744956239197379 1102 30-31 0.5257309325262298 1102 32-33 0.5090028034356209 1102 34-35 0.5280231608808279 1102 36-37 0.5357281733286499 1102 38-39 0.6243724726478774 1102 40-41 0.5462114733103007 1102 42-43 0.5288946206472431 1102 44-45 0.45673595898066566 1102 46-47 0.41575209103994126 1102 48-49 0.38781758570294045 1102 50-51 0.5229948956361454 1102 52-53 0.43013316841722826 1102 54-55 0.3855837323941813 1102 56-57 0.45396146871489407 1102 58-59 0.4025418704951278 1102 60-61 0.4143910758532101 1102 62-63 0.43407160216903407 1102 64-65 0.4163884964531803 1102 66-67 0.404932697067764 1102 68-69 0.4641364966932571 1102 70-71 0.40254345748046205 1102 72-73 0.4620680404701005 1102 74-75 0.45654445577746827 1102 76-77 0.35680873822327897 1102 78-79 0.34828468819937797 1102 80-81 0.4253595918438471 1102 82-83 0.45104807334745445 1102 84-85 0.38947192500874195 1102 86-87 0.41733655066689934 1102 88-89 0.37851001562978936 1102 90-91 0.32266296009932205 1102 92-93 0.3245625487706576 1102 94-95 0.46435354997953837 1102 96-97 0.46519685776876685 1102 98-99 0.466933065719374 1102 100 0.45847792043871394 1103 1 0.2683417566682671 1103 2 0.3405036846356353 1103 3 0.4086162993894149 1103 4 0.4289208999658314 1103 5 0.4537387483472557 1103 6 0.3975291849205149 1103 7 0.39124792120443175 1103 8 0.41316896160352456 1103 9 0.47162488329157526 1103 10-11 0.5098197011249823 1103 12-13 0.46517426876238943 1103 14-15 0.5477370799173684 1103 16-17 0.5494172703225075 1103 18-19 0.5163806906374901 1103 20-21 0.5493282457370725 1103 22-23 0.5346770391361915 1103 24-25 0.6294466673049612 1103 26-27 0.5580415478364316 1103 28-29 0.5464772582499577 1103 30-31 0.561308024297972 1103 32-33 0.4814605617126375 1103 34-35 0.4869037888449341 1103 36-37 0.46996638103583166 1103 38-39 0.5430009048694231 1103 40-41 0.56755491005039 1103 42-43 0.5149171238720598 1103 44-45 0.5213457115824909 1103 46-47 0.41894406259334716 1103 48-49 0.4982937778997112 1103 50-51 0.48247234965472074 1103 52-53 0.46863952951666477 1103 54-55 0.4492056668155797 1103 56-57 0.45648770180144993 1103 58-59 0.5075264078585775 1103 60-61 0.5400068613523317 1103 62-63 0.523639230478004 1103 64-65 0.5329915501181475 1103 66-67 0.4453830787257118 1103 68-69 0.44492781914915014 1103 70-71 0.48526906483508014 1103 72-73 0.400685488458592 1103 74-75 0.3830592541810631 1103 76-77 0.40393872594229663 1103 78-79 0.46749760125732465 1103 80-81 0.4617303105121877 1103 82-83 0.41487568110916584 1103 84-85 0.4414139181411869 1103 86-87 0.4914942354346685 1103 88-89 0.41548428498357737 1103 90-91 0.4413969188434095 1103 92-93 0.38442300565212406 1103 94-95 0.4612329598821283 1103 96-97 0.5169532510862069 1103 98-99 0.5334839683655872 1103 100 0.5262308057068736 1104 1 0.2512497259724036 1104 2 0.203557328835565 1104 3 0.24339609455492806 1104 4 0.2673581993596983 1104 5 0.34287284246813243 1104 6 0.2715668358007548 1104 7 0.364434154386565 1104 8 0.4028886173123851 1104 9 0.44865390390548043 1104 10-11 0.43448592987043355 1104 12-13 0.5348830511517519 1104 14-15 0.49778848681112464 1104 16-17 0.5191733505023208 1104 18-19 0.5041982625424879 1104 20-21 0.5352767216045748 1104 22-23 0.48451850820671183 1104 24-25 0.4836117176285484 1104 26-27 0.48256823947954786 1104 28-29 0.44436483433302243 1104 30-31 0.4794264112996416 1104 32-33 0.4540818650282503 1104 34-35 0.4335134338011599 1104 36-37 0.4671420190531421 1104 38-39 0.48487433414312875 1104 40-41 0.50536555682325 1104 42-43 0.4935474879041237 1104 44-45 0.5156505518763055 1104 46-47 0.46313065841102485 1104 48-49 0.4322311206834044 1104 50-51 0.3829528561261313 1104 52-53 0.33342250277185315 1104 54-55 0.337345472877395 1104 56-57 0.2730349171224873 1104 58-59 0.3343666108655228 1104 60-61 0.34845713171984016 1104 62-63 0.4380572406490373 1104 64-65 0.4181144123068634 1104 66-67 0.35812762065838655 1104 68-69 0.3514215241753078 1104 70-71 0.37798181437690204 1104 72-73 0.3651826060519312 1104 74-75 0.40102954105394417 1104 76-77 0.3952196684958835 1104 78-79 0.3807860390452156 1104 80-81 0.38067034576426906 1104 82-83 0.3533361983734338 1104 84-85 0.29540288045754437 1104 86-87 0.23807376612334608 1104 88-89 0.19525634756378452 1104 90-91 0.30666706508726094 1104 92-93 0.3482915166452649 1104 94-95 0.2872088719162882 1104 96-97 0.31129217107593377 1104 98-99 0.33284909753798075 1104 100 0.31145918277198703 1105 1 0.2087242600312571 1105 2 0.1797300998525877 1105 3 0.26898675881368916 1105 4 0.25363393579655025 1105 5 0.16200465945172482 1105 6 0.26366167257872775 1105 7 0.2502515235551712 1105 8 0.26555706236536736 1105 9 0.33388424821208673 1105 10-11 0.3696334533101151 1105 12-13 0.34513670727006485 1105 14-15 0.3533693542072456 1105 16-17 0.38107181723430017 1105 18-19 0.38779973089153685 1105 20-21 0.3726267686216431 1105 22-23 0.3097196586495343 1105 24-25 0.3284121016340862 1105 26-27 0.33470446701856815 1105 28-29 0.34028870995614824 1105 30-31 0.27539553347713763 1105 32-33 0.295894009203451 1105 34-35 0.24956887853544885 1105 36-37 0.31082500988974004 1105 38-39 0.3433726206757939 1105 40-41 0.34745626239408267 1105 42-43 0.30035847140683813 1105 44-45 0.2679280273546354 1105 46-47 0.19616693827866527 1105 48-49 0.19548619715008186 1105 50-51 0.2513251211686409 1105 52-53 0.20282048752967086 1105 54-55 0.1256179594313025 1105 56-57 0.2067860925492795 1105 58-59 0.18908228545230088 1105 60-61 0.11411932487934706 1105 62-63 0.13535975911160136 1105 64-65 0.12671440522480282 1105 66-67 0.13505345171955696 1105 68-69 0.08936410433055642 1105 70-71 0.04416847411887659 1105 72-73 0.049831040324427534 1105 74-75 0.07520123402262868 1105 76-77 0.01638600663322265 1105 78-79 0.07171666230666673 1105 80-81 0.16042388974982202 1105 82-83 0.1691284261957371 1105 84-85 0.17082062418893074 1105 86-87 0.12912006318468272 1105 88-89 0.07446535910362329 1105 90-91 0.11020809977759427 1105 92-93 0.06212633918040922 1105 94-95 0.0424580623964097 1105 96-97 0.14819644117032382 1105 98-99 0.09234005412882951 1105 100 -0.006447042760449051 1106 1 0.12593626588272855 1106 2 0.12262091145263909 1106 3 0.13469258845096022 1106 4 0.20687027425964288 1106 5 0.12490570501619658 1106 6 0.1716617770296125 1106 7 0.2486758708343615 1106 8 0.2319708801063456 1106 9 0.29352553720268304 1106 10-11 0.30584868658283426 1106 12-13 0.2638348047638601 1106 14-15 0.300944232435846 1106 16-17 0.22586124591573764 1106 18-19 0.2906373503225197 1106 20-21 0.22572126500447354 1106 22-23 0.30199301550654667 1106 24-25 0.2712004624718958 1106 26-27 0.28414107116471854 1106 28-29 0.23584476924646935 1106 30-31 0.29053985626593715 1106 32-33 0.2435643933215772 1106 34-35 0.24428044130404203 1106 36-37 0.3628147714805863 1106 38-39 0.3307315078071582 1106 40-41 0.39819511351213066 1106 42-43 0.3143595014703564 1106 44-45 0.3101924114360983 1106 46-47 0.34109393099877394 1106 48-49 0.3842426704659445 1106 50-51 0.30289163443696054 1106 52-53 0.3588698327948521 1106 54-55 0.3535851840768274 1106 56-57 0.3168299897007074 1106 58-59 0.2872581174063882 1106 60-61 0.2610521079565231 1106 62-63 0.42506373530124364 1106 64-65 0.33145547536857123 1106 66-67 0.37039996475452597 1106 68-69 0.355719085109925 1106 70-71 0.3683647439999689 1106 72-73 0.2979866210296791 1106 74-75 0.3869026222749028 1106 76-77 0.29033145549145445 1106 78-79 0.268300007168488 1106 80-81 0.3565401502525525 1106 82-83 0.27256613818797604 1106 84-85 0.29761005398154694 1106 86-87 0.2412594784486224 1106 88-89 0.1974951681640711 1106 90-91 0.20553281146814584 1106 92-93 0.1273669179049861 1106 94-95 0.18142529870931767 1106 96-97 0.27436955517902106 1106 98-99 0.2220708875302222 1106 100 0.2028878662256055 1107 1 0.16978074740353222 1107 2 0.18338895750126127 1107 3 0.24326695890034244 1107 4 0.19680866335158242 1107 5 0.12975281395709715 1107 6 0.19912481347480337 1107 7 0.21379147351277794 1107 8 0.20974963643909206 1107 9 0.27563896585814973 1107 10-11 0.27971365795738024 1107 12-13 0.2912882731160309 1107 14-15 0.3560995046453712 1107 16-17 0.32560747399406864 1107 18-19 0.3603914666411683 1107 20-21 0.42848291021631724 1107 22-23 0.4124910619302753 1107 24-25 0.4107270701969412 1107 26-27 0.48901704747716224 1107 28-29 0.4166904255768813 1107 30-31 0.4526386827067057 1107 32-33 0.4115553710447699 1107 34-35 0.4483049660661038 1107 36-37 0.3159515566122053 1107 38-39 0.3221097861701736 1107 40-41 0.4867581979608744 1107 42-43 0.485331864359857 1107 44-45 0.3951515866664792 1107 46-47 0.3005532141420204 1107 48-49 0.3544858468118761 1107 50-51 0.4254224820232153 1107 52-53 0.38325814080263854 1107 54-55 0.32085273785793333 1107 56-57 0.301817923574319 1107 58-59 0.2720917114158965 1107 60-61 0.2667268569435848 1107 62-63 0.29622718823030425 1107 64-65 0.28469517186430693 1107 66-67 0.29987099599380684 1107 68-69 0.2475843032977849 1107 70-71 0.28502806727048835 1107 72-73 0.23840213721470604 1107 74-75 0.26822825981705023 1107 76-77 0.19624736391393327 1107 78-79 0.17767842361647368 1107 80-81 0.26153170477953935 1107 82-83 0.225499710071265 1107 84-85 0.20265994125745124 1107 86-87 0.14584819451383169 1107 88-89 0.13973813575161032 1107 90-91 0.23101155589514377 1107 92-93 0.20832309658259973 1107 94-95 0.22300040253128017 1107 96-97 0.2483278698246707 1107 98-99 0.3426846915702413 1107 100 0.26250698001360817 1108 1 0.05777070920186844 1108 2 0.07229066751835234 1108 3 0.09169808900932708 1108 4 0.05144179347886535 1108 5 0.15699718158779064 1108 6 0.1390038237020761 1108 7 0.08828647382023291 1108 8 0.09728149897357241 1108 9 0.1181076355947468 1108 10-11 0.16665486559675458 1108 12-13 0.2187125462525401 1108 14-15 0.2166346097950509 1108 16-17 0.1905109812295791 1108 18-19 0.1760681638450805 1108 20-21 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-0.7954171354069004 2215 52-53 -0.7159894304620877 2215 54-55 -0.8143814045179312 2215 56-57 -0.7709454140631777 2215 58-59 -0.8033742018353536 2215 60-61 -0.7371656951097094 2215 62-63 -0.7750535131100875 2215 64-65 -0.8500970137621522 2215 66-67 -0.7891521617739699 2215 68-69 -0.7552151988596165 2215 70-71 -0.721502649698202 2215 72-73 -0.609417790068477 2215 74-75 -0.679101487216915 2215 76-77 -0.7631598030544637 2215 78-79 -0.677066326480368 2215 80-81 -0.735392951746185 2215 82-83 -0.7229861520736982 2215 84-85 -0.7666910197004881 2215 86-87 -0.8040550824899846 2215 88-89 -0.7922438390507303 2215 90-91 -0.834968890162223 2215 92-93 -0.8030061829131867 2215 94-95 -0.8916924022520725 2215 96-97 -0.9787906004588471 2215 98-99 -0.987402025170482 2215 100 -1.021806258423826 2216 1 -0.7144865480909601 2216 2 -0.8924227824728952 2216 3 -0.9833764741934381 2216 4 -0.9905272785935963 2216 5 -0.9918290176518596 2216 6 -1.0142594477751672 2216 7 -0.9704303808132408 2216 8 -0.8965959627228415 2216 9 -1.0418267011732496 2216 10-11 -1.0915445623335671 2216 12-13 -1.1573689585507054 2216 14-15 -1.2261274107773517 2216 16-17 -1.3641655162026893 2216 18-19 -1.4931349546807482 2216 20-21 -1.4156099785284084 2216 22-23 -1.402632700396559 2216 24-25 -1.4054272475886194 2216 26-27 -1.4013370872524433 2216 28-29 -1.3753668666859014 2216 30-31 -1.5325556666235371 2216 32-33 -1.564064872731656 2216 34-35 -1.4685666906301478 2216 36-37 -1.5329148265137746 2216 38-39 -1.4302485823005355 2216 40-41 -1.5312576229847252 2216 42-43 -1.541852484156884 2216 44-45 -1.6800532536925132 2216 46-47 -1.4532173404738344 2216 48-49 -1.630492096437031 2216 50-51 -1.5203077209289404 2216 52-53 -1.8512794480591452 2216 54-55 -1.6879915034101387 2216 56-57 -1.6470123977408804 2216 58-59 -1.5695996978554945 2216 60-61 -1.5687191796438924 2216 62-63 -1.6328391595147238 2216 64-65 -1.6698916568645146 2216 66-67 -1.59651639002616 2216 68-69 -1.5396574107322891 2216 70-71 -1.4353957012662129 2216 72-73 -1.4827789058708234 2216 74-75 -1.678152100488802 2216 76-77 -1.716936937412548 2216 78-79 -1.718312716823032 2216 80-81 -1.6621159215562429 2216 82-83 -1.6491313225490636 2216 84-85 -1.609396336766899 2216 86-87 -1.6993958078916975 2216 88-89 -1.6758339927415982 2216 90-91 -1.8048906062522363 2216 92-93 -1.8849945864883182 2216 94-95 -1.7997110840828263 2216 96-97 -1.7989165065812784 2216 98-99 -1.8164055905582543 2216 100 -1.9856961803955926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76959.0 3 13834.0 4 8955.0 5 7390.0 6 6378.0 7 6746.0 8 7038.0 9 8452.0 10 9496.0 11 10071.0 12 11147.0 13 12326.0 14 13199.0 15 13528.0 16 13637.0 17 14108.0 18 13077.0 19 12509.0 20 12541.0 21 13343.0 22 15004.0 23 17910.0 24 22191.0 25 27487.0 26 32653.0 27 37151.0 28 44059.0 29 55088.0 30 64633.0 31 75968.0 32 95454.0 33 125484.0 34 170710.0 35 245263.0 36 403138.0 37 667033.0 38 756054.0 39 232211.0 40 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7013795974589 20.20453163626199 14.016506030638794 23.07758273564031 2 16.27802012377933 20.69614753879595 39.167529128473696 23.858303208951018 3 18.1176829252018 25.634127947477946 30.529660055640683 25.71852907167957 4 13.101679047769576 17.85648517040314 38.28739559143576 30.75444019039152 5 13.140480942847969 37.061298224835575 34.822496901921 14.975723930395453 6 29.846871904968065 37.79793852339918 18.29845152030934 14.056738051323414 7 26.219760841849027 32.38184519105042 22.991710042168968 18.40668392493159 8 24.42724074451883 35.17503926136445 21.411202892293385 18.986517101823342 9 24.516327998161476 17.510315736409396 22.110029579438212 35.86332668599091 10-11 23.36542107055699 27.04407159742248 28.99196227463076 20.598545057389767 12-13 24.108967581041465 25.02796859688166 28.54393816526894 22.31912565680793 14-15 21.909006342043323 27.282855369564064 25.907278122634185 24.900860165758424 16-17 21.2659346459886 30.088867043426752 26.443605717085944 22.201592593498702 18-19 21.20072217870587 29.0274236050785 28.386035565388617 21.38581865082701 20-21 22.47355511142802 28.546779217856123 27.657095782533098 21.322569888182763 22-23 22.129053972904565 28.64141772842589 27.59115461774444 21.638373680925106 24-25 22.005472316876595 28.418993295944755 27.728511043757038 21.84702334342162 26-27 21.67823562361022 28.340919947799588 27.874959190626697 22.105885237963495 28-29 21.773292916400766 28.340609527831223 27.962427317133347 21.923670238634667 30-31 22.151390866219625 28.885794681356757 27.375008562529896 21.587805889893723 32-33 21.63956088470117 28.566529253471668 27.898995593219183 21.894914268607977 34-35 21.82370140171812 28.014805962713762 27.95377918269188 22.207713452876234 36-37 22.34046228384702 28.482634145806244 27.349024111750204 21.82787945859653 38-39 22.044263647112658 28.590304738140265 27.576403990893176 21.789027623853897 40-41 21.849826001473836 28.27491618860936 27.759177736491946 22.116080073424857 42-43 21.722260928020905 28.403150307704923 27.675265482323592 22.199323281950583 44-45 22.010730952736832 28.54341789029896 27.504739128474863 21.94111202848934 46-47 21.97868092683164 28.761531718230465 27.298900001304858 21.960887353633034 48-49 21.762175783116827 28.407612654899232 27.617560013884972 22.212651548098965 50-51 21.806632425285837 28.254076561378945 27.589250513494683 22.35004049984054 52-53 21.78361526106097 28.357945388948174 27.509100433407628 22.34933891658323 54-55 21.95013170915274 28.742664661646998 27.362869317179307 21.944334312020956 56-57 21.674263989431513 28.46521920874851 27.41579961395784 22.444717187862135 58-59 21.535371823609307 28.021904951534914 27.766365963084432 22.676357261771347 60-61 21.953123459427786 28.50284514266338 27.290062216692245 22.253969181216586 62-63 21.78849859034053 28.493062613078944 27.35000330655371 22.368435490026812 64-65 21.783117143683754 28.150738062017584 27.614387638300897 22.45175715599776 66-67 21.63818734257095 28.227699565322347 27.377234254426103 22.756878837680606 68-69 22.07703473932532 28.380126419176094 27.32440916160926 22.21842967988933 70-71 22.071168904736165 28.0354888052038 27.47014733370554 22.423194956354493 72-73 21.700821756605947 28.2209587822735 27.735182564963672 22.34303689615688 74-75 21.675835068998047 28.159198934015677 27.641213117680884 22.523752879305388 76-77 21.824936133240218 28.172572951926828 27.324179221322233 22.67831169351072 78-79 21.888821639741142 28.273194110793447 27.501265493210227 22.336718756255184 80-81 21.6692065112352 28.04090719086172 27.63427009285941 22.65561620504368 82-83 21.529292205608574 28.07208007605731 27.776753057504994 22.62187466082912 84-85 21.653979888272133 27.9659505342414 27.597868639022195 22.78220093846427 86-87 21.84031815788471 28.057199262226256 27.539633690204717 22.56284888968432 88-89 21.57138305082635 27.948018734322638 27.663552039315565 22.81704617553545 90-91 21.507470390303666 27.781269688333264 27.711179407247073 23.000080514115993 92-93 21.642265496865505 27.96825381763426 27.49930240716233 22.89017827833791 94-95 21.563675039356102 27.718988895477274 27.70852062419959 23.00881544096703 96-97 21.560687265005193 27.768138359248063 27.70445493363431 22.96671944211243 98-99 21.516926209740973 27.74170466193025 27.672566651162633 23.068802477166145 100 21.620195074824743 27.711590770463417 27.60596700161638 23.062247153095456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2338.0 1 1928.0 2 1783.0 3 2026.0 4 1859.5 5 1579.5 6 1327.0 7 1217.0 8 1270.5 9 1267.5 10 1139.5 11 1044.5 12 1051.5 13 1089.0 14 1133.0 15 1211.5 16 1350.0 17 1565.0 18 1888.0 19 2391.0 20 3199.5 21 4328.5 22 6199.0 23 9075.0 24 12626.5 25 17179.5 26 23139.0 27 30245.5 28 39277.0 29 48958.5 30 57199.5 31 64824.5 32 72479.0 33 79846.5 34 86281.0 35 91909.5 36 97356.0 37 102447.5 38 107113.5 39 110345.0 40 113240.5 41 115395.5 42 114823.5 43 114005.0 44 113269.0 45 112389.0 46 112264.0 47 110470.5 48 108239.5 49 107563.5 50 105469.5 51 101493.0 52 96022.5 53 91369.5 54 88869.0 55 86110.5 56 81191.0 57 74073.0 58 67351.0 59 61956.5 60 55753.0 61 48203.5 62 40941.0 63 34128.0 64 27742.0 65 22255.5 66 17697.5 67 13871.5 68 10779.0 69 8249.0 70 6158.0 71 4683.5 72 3521.0 73 2636.0 74 1996.5 75 1469.0 76 1020.5 77 676.0 78 511.5 79 376.5 80 241.0 81 165.5 82 137.5 83 97.5 84 67.0 85 50.5 86 41.5 87 34.5 88 24.5 89 18.5 90 14.0 91 13.5 92 16.0 93 11.0 94 5.5 95 8.0 96 8.5 97 7.0 98 7.0 99 6.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07430585845060128 2 0.012364542288068929 3 0.01642675402299805 4 0.010022099024861626 5 0.008776749441890655 6 0.006137794373214073 7 0.018858150827846133 8 0.008154074650405168 9 0.008065121108764384 10-11 0.011460181281387627 12-13 0.013684019822407219 14-15 0.008598842358609086 16-17 0.011104367114824492 18-19 0.007457271907552363 20-21 0.011638088364669194 22-23 0.011623262774395732 24-25 0.014336345794439632 26-27 0.0047441888875084616 28-29 0.010763378538534821 30-31 0.008776749441890655 32-33 0.01060029704552672 34-35 0.0076203534005604675 36-37 0.009236342740368038 38-39 0.016901172911748894 40-41 0.011623262774395732 42-43 0.0071607601020830846 44-45 0.01835408075854836 46-47 0.016100591036981842 48-49 0.016500881974365368 50-51 0.009236342740368038 52-53 0.009414249823649605 54-55 0.010200006108143193 56-57 0.01884332523757267 58-59 0.0020014546869176323 60-61 0.006538085310597599 62-63 0.013520938329399115 64-65 0.02305379287523643 66-67 0.011652913954942659 68-69 0.012809309996272847 70-71 0.02311309523633029 72-73 0.018502336661283 74-75 0.018976755550033846 76-77 0.008658144719702943 78-79 0.010200006108143193 80-81 0.018428208709915683 82-83 0.010466866733065544 84-85 0.009947971073494306 86-87 0.015136927669206685 88-89 0.01326890329475023 90-91 0.013861926905688786 92-93 0.006997678609074981 94-95 0.013417159197484868 96-97 0.0118011698576773 98-99 0.009088086837633397 100 0.005930236109385577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3372547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.64963472444403 #Duplication Level Percentage of deduplicated Percentage of total 1 81.37821824423266 55.05206738748426 2 11.696246677767542 15.824936307959325 3 3.1118121657415125 6.315388690304832 4 1.262199120181792 3.415492377192514 5 0.6681775724379412 2.2600984353246227 6 0.41374878703533646 1.6793972586373374 7 0.2904839946545766 1.375579529217562 8 0.20340274717750512 1.100809723880533 9 0.14932206074795634 0.9091424579310441 >10 0.7793859821787862 9.142106971112602 >50 0.036166524180451715 1.6542121445843863 >100 0.010660691047426212 1.1104326805458717 >500 1.3157446300862737E-4 0.060763916431484266 >1k 4.385815348543591E-5 0.09957211939355312 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.9651180546927887E-5 0.0 5 0.0 0.0 0.0 2.9651180546927887E-5 0.0 6 0.0 0.0 0.0 2.9651180546927887E-5 0.0 7 0.0 0.0 0.0 2.9651180546927887E-5 0.0 8 0.0 0.0 0.0 2.9651180546927887E-5 0.0 9 2.9651180546927887E-5 0.0 0.0 2.9651180546927887E-5 0.0 10-11 2.9651180546927887E-5 5.9302361093855774E-5 0.0 4.447677082039183E-5 0.0 12-13 2.9651180546927887E-5 8.895354164078366E-5 0.0 7.412795136731971E-5 0.0 14-15 2.9651180546927887E-5 1.037791319142476E-4 0.0 8.895354164078366E-5 0.0 16-17 2.9651180546927887E-5 1.1860472218771155E-4 0.0 1.3343031246117548E-4 0.0 18-19 2.9651180546927887E-5 1.1860472218771155E-4 0.0 1.7790708328156731E-4 0.0 20-21 2.9651180546927887E-5 1.1860472218771155E-4 0.0 1.9273267355503126E-4 0.0 22-23 2.9651180546927887E-5 1.1860472218771155E-4 0.0 4.7441888875084613E-4 0.0 24-25 2.9651180546927887E-5 1.1860472218771155E-4 0.0 0.001304651944064827 0.0 26-27 2.9651180546927887E-5 1.1860472218771155E-4 0.0 0.002090408228558416 0.0 28-29 4.447677082039183E-5 1.1860472218771155E-4 0.0 0.004788665658328853 0.0 30-31 5.9302361093855774E-5 1.3343031246117548E-4 0.0 0.012053204892326187 0.0 32-33 5.9302361093855774E-5 1.4825590273463942E-4 0.0 0.028331703012589596 0.0 34-35 5.9302361093855774E-5 1.4825590273463942E-4 0.0 0.05672270838627304 0.0 36-37 5.9302361093855774E-5 1.9273267355503126E-4 0.0 0.10054715323463245 0.0 38-39 5.9302361093855774E-5 2.52035034648887E-4 0.0 0.1573143383917259 0.0 40-41 5.9302361093855774E-5 2.6686062492235096E-4 0.0 0.22211699347703678 0.0 42-43 5.9302361093855774E-5 2.6686062492235096E-4 0.0 0.2891731382839142 0.0 44-45 5.9302361093855774E-5 2.6686062492235096E-4 0.0 0.37560632957820894 0.0 46-47 5.9302361093855774E-5 2.6686062492235096E-4 0.0 0.4780215071872979 0.0 48-49 8.895354164078366E-5 2.6686062492235096E-4 0.0 0.5831942445872511 0.0 50-51 8.895354164078366E-5 2.6686062492235096E-4 0.0 0.6897457618826365 0.0 52-53 8.895354164078366E-5 2.6686062492235096E-4 0.0 0.7957190811573567 0.0 54-55 8.895354164078366E-5 2.6686062492235096E-4 0.0 0.9241976464671953 0.0 56-57 8.895354164078366E-5 2.6686062492235096E-4 0.0 1.0736988987848055 0.0 58-59 8.895354164078366E-5 2.6686062492235096E-4 0.0 1.2415542318609645 0.0 60-61 8.895354164078366E-5 3.2616298601620674E-4 0.0 1.4144206144495541 0.0 62-63 8.895354164078366E-5 3.2616298601620674E-4 0.0 1.5901779871414692 2.9651180546927887E-5 64-65 8.895354164078366E-5 3.2616298601620674E-4 0.0 1.7872990354174456 2.9651180546927887E-5 66-67 8.895354164078366E-5 3.409885762896707E-4 0.0 2.0156427768093375 2.9651180546927887E-5 68-69 8.895354164078366E-5 3.5581416656313463E-4 0.0 2.2642382745147804 2.9651180546927887E-5 70-71 8.895354164078366E-5 3.854653471100625E-4 0.0 2.5362878560328443 2.9651180546927887E-5 72-73 8.895354164078366E-5 4.151165276569904E-4 0.0 2.8276551816772306 2.9651180546927887E-5 74-75 1.1860472218771155E-4 4.2994211793045435E-4 0.0 3.126227744194521 2.9651180546927887E-5 76-77 1.1860472218771155E-4 4.744188887508462E-4 0.0 3.4427837477135235 2.9651180546927887E-5 78-79 1.1860472218771155E-4 4.744188887508462E-4 0.0 3.79920576347787 2.9651180546927887E-5 80-81 1.1860472218771155E-4 4.744188887508462E-4 0.0 4.182817911803749 2.9651180546927887E-5 82-83 1.1860472218771155E-4 4.744188887508462E-4 0.0 4.582071057868134 2.9651180546927887E-5 84-85 1.1860472218771155E-4 4.744188887508462E-4 0.0 4.9843486243483035 2.9651180546927887E-5 86-87 1.1860472218771155E-4 4.892444790243101E-4 0.0 5.413771846619187 2.9651180546927887E-5 88 1.1860472218771155E-4 5.337212498447019E-4 0.0 5.758466820477224 2.9651180546927887E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3595 0.0 45.91446 1 GTATCAA 6145 0.0 35.279327 1 ATCAACG 6520 0.0 31.859968 3 TCAACGC 6670 0.0 31.214401 4 CAACGCA 7140 0.0 29.226793 5 TATCAAC 7555 0.0 28.117785 2 AACGCAG 7590 0.0 27.742495 6 ACGCAGA 8325 0.0 24.785406 7 GTGGTAT 2055 0.0 24.714577 1 CGCAGAG 8320 0.0 24.233215 8 TGGTATC 2135 0.0 22.893518 2 GTACATG 8995 0.0 21.644144 1 TACATGG 9140 0.0 20.619366 2 GCAGAGT 9475 0.0 20.386967 9 ACATGGG 9205 0.0 19.452341 3 TATAACG 200 1.4093446E-5 18.7991 2 CAGAGTA 9075 0.0 18.566603 10-11 AGAGTAC 8325 0.0 17.218906 10-11 GTACACG 330 1.3722456E-8 17.10049 1 TACCTGG 2175 0.0 17.070448 2 >>END_MODULE