FastQCFastQC Report
Wed 25 May 2016
SRR2049460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372547
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131070.38863802342858383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127630.3784380173204406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73790.21879606125578088No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.12785589051835305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.10730762239933203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71050.058.5030981
GTATCAA126900.039.2469831
GTGGTAT27900.037.119381
TATAACG3300.034.186482
TGGTATC30950.032.3501322
ATCAACG156000.031.6389313
TCAACGC158300.031.2089354
TATCAAC162350.030.4303932
CAACGCA164200.030.1734225
AACGCAG170550.029.2381536
ATAACGC4300.027.329313
ACGCAGA191950.025.9050147
CGCAGAG194100.025.6422848
GCAGAGT209250.023.4712989
CAGAGTA204050.021.75462710-11
TAACGCA6200.021.2287034
GAGTACT165200.021.01003512-13
AGAGTAC198000.020.4729410-11
GTACATG85550.020.0842251
CTGTCGC9300.019.2037899
Produced by FastQC (version 0.11.5)