##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049460_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3372547 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81562273261129 31.0 31.0 34.0 30.0 34.0 2 31.89403705863847 33.0 31.0 34.0 30.0 34.0 3 31.96627830538759 33.0 31.0 34.0 30.0 34.0 4 35.73095022841787 37.0 35.0 37.0 33.0 37.0 5 35.82857081013252 37.0 35.0 37.0 35.0 37.0 6 35.92301011668629 37.0 35.0 37.0 35.0 37.0 7 35.88476898913492 37.0 35.0 37.0 35.0 37.0 8 35.8969066998918 37.0 35.0 37.0 35.0 37.0 9 37.608077515302234 39.0 37.0 39.0 35.0 39.0 10-11 37.59063313276286 39.0 37.0 39.0 35.0 39.0 12-13 37.587131773107984 39.0 37.0 39.0 35.0 39.0 14-15 38.87234722006839 40.0 38.0 41.0 35.0 41.0 16-17 38.876931737348656 40.0 38.0 41.0 35.0 41.0 18-19 38.95962428396105 40.0 38.5 41.0 35.0 41.0 20-21 38.978841807097126 40.0 38.5 41.0 35.0 41.0 22-23 38.939974001844895 40.0 38.0 41.0 35.0 41.0 24-25 38.917795511819406 40.0 38.0 41.0 35.0 41.0 26-27 38.811193884028896 40.0 38.0 41.0 35.0 41.0 28-29 38.71626044055131 40.0 38.0 41.0 35.0 41.0 30-31 38.57932758238803 40.0 38.0 41.0 35.0 41.0 32-33 38.40935886141838 40.0 38.0 41.0 34.0 41.0 34-35 38.259759315437265 40.0 38.0 41.0 34.0 41.0 36-37 38.10153957824754 40.0 38.0 41.0 34.0 41.0 38-39 37.97279607963951 40.0 38.0 41.0 33.0 41.0 40-41 37.86078637302905 40.0 37.5 41.0 33.0 41.0 42-43 37.69665241136743 40.0 37.0 41.0 33.0 41.0 44-45 37.49387599342574 40.0 37.0 41.0 33.0 41.0 46-47 37.20308731056973 40.0 37.0 41.0 32.0 41.0 48-49 36.91329935505717 40.0 37.0 41.0 31.0 41.0 50-51 36.73534720198117 40.0 36.0 41.0 31.0 41.0 52-53 36.60535687123115 40.0 36.0 41.0 31.0 41.0 54-55 36.4517441862189 39.5 36.0 41.0 31.0 41.0 56-57 36.288204137703644 39.0 35.0 41.0 30.5 41.0 58-59 36.09686744172876 39.0 35.0 41.0 30.0 41.0 60-61 35.89093480387375 39.0 35.0 41.0 30.0 41.0 62-63 35.64702152408847 38.5 35.0 40.5 29.5 41.0 64-65 35.40684562735523 38.0 35.0 40.0 29.0 41.0 66-67 35.13373898125067 37.0 35.0 40.0 29.0 41.0 68-69 34.82655793974109 37.0 34.5 40.0 29.0 41.0 70-71 34.534694105078444 36.0 34.0 39.0 28.5 41.0 72-73 34.19939054963504 36.0 34.0 39.0 28.0 41.0 74-75 33.885096338168154 35.5 34.0 39.0 28.0 40.5 76-77 33.57828727071854 35.0 34.0 37.5 28.0 40.0 78-79 33.28288842231109 35.0 34.0 37.0 27.0 39.0 80-81 32.988587557119295 35.0 34.0 37.0 27.0 39.0 82-83 32.733141450660284 35.0 34.0 36.0 27.0 38.5 84-85 32.464325330380866 35.0 33.0 36.0 26.0 37.0 86-87 32.233583549762244 35.0 33.0 35.5 26.0 37.0 88-89 32.02771718229575 35.0 33.0 35.0 26.0 36.5 90-91 31.84812576370322 35.0 33.0 35.0 26.0 36.0 92-93 31.689651915896206 35.0 33.0 35.0 25.0 36.0 94-95 31.531724835858476 35.0 33.0 35.0 25.0 36.0 96-97 31.391599138573902 35.0 33.0 35.0 25.0 35.5 98-99 31.2578312474222 35.0 33.0 35.0 24.5 35.0 100 31.15064400881589 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3130477682090884 1101 2 0.34967463916766306 1101 3 0.4916865545364715 1101 4 0.23935043412530632 1101 5 0.25776032365978097 1101 6 0.2209929616325752 1101 7 0.24530996081701772 1101 8 0.27253664127017885 1101 9 0.3673646941228341 1101 10-11 0.3432212286630971 1101 12-13 0.28474253937478977 1101 14-15 0.43185611061269924 1101 16-17 0.4078499682944141 1101 18-19 0.4937246759262237 1101 20-21 0.469586606106553 1101 22-23 0.49017179972102554 1101 24-25 0.4458864829005691 1101 26-27 0.41604038609100513 1101 28-29 0.4629629101966799 1101 30-31 0.42122899055105734 1101 32-33 0.45108605369016175 1101 34-35 0.40435584261814483 1101 36-37 0.45042218274495127 1101 38-39 0.3838134192430047 1101 40-41 0.35458734324384267 1101 42-43 0.37433372466647086 1101 44-45 0.310502580393738 1101 46-47 0.26014706333847215 1101 48-49 0.231872101283912 1101 50-51 0.2866419454253304 1101 52-53 0.22578107446575757 1101 54-55 0.25441057103086706 1101 56-57 0.28655463462015973 1101 58-59 0.25763303223897793 1101 60-61 0.16024327606894673 1101 62-63 0.2215489438178153 1101 64-65 0.1622953417645121 1101 66-67 0.18671704629075947 1101 68-69 0.17332445213404668 1101 70-71 0.1980214213290239 1101 72-73 0.2157884169073867 1101 74-75 0.16294412138613978 1101 76-77 0.20469606113777417 1101 78-79 0.13926692959105935 1101 80-81 0.05382366816063211 1101 82-83 0.08437653919555288 1101 84-85 0.08238176850181844 1101 86-87 0.11310954546799223 1101 88-89 0.1556181285240683 1101 90-91 0.10883511229232923 1101 92-93 0.15920925335965208 1101 94-95 0.16732905915359453 1101 96-97 0.11981576346427758 1101 98-99 0.22374057596622876 1101 100 0.2063758867353478 1102 1 0.24689728153406776 1102 2 0.2669294578597068 1102 3 0.34583381472008057 1102 4 0.21082944790113345 1102 5 0.23555457452451378 1102 6 0.21397542658992563 1102 7 0.2551544451039973 1102 8 0.20498792455855153 1102 9 0.2603141989460269 1102 10-11 0.2691499861916782 1102 12-13 0.22980132526081576 1102 14-15 0.35031895800811697 1102 16-17 0.30522655939568466 1102 18-19 0.3403136580276609 1102 20-21 0.3936852595154505 1102 22-23 0.343709108959537 1102 24-25 0.310854865043801 1102 26-27 0.2897186197963606 1102 28-29 0.3220550699984628 1102 30-31 0.3310610572017225 1102 32-33 0.3770155231911474 1102 34-35 0.35436964231853096 1102 36-37 0.3847072975379362 1102 38-39 0.3246734944691667 1102 40-41 0.3957833438016962 1102 42-43 0.2980453373132761 1102 44-45 0.438833386225042 1102 46-47 0.37374360030471365 1102 48-49 0.404084048767146 1102 50-51 0.3860023429237103 1102 52-53 0.3890419792654143 1102 54-55 0.430531351704424 1102 56-57 0.3209695377940065 1102 58-59 0.3780595832813276 1102 60-61 0.3340735158821957 1102 62-63 0.3426376082539164 1102 64-65 0.2854240374771422 1102 66-67 0.266301803045387 1102 68-69 0.29111176336189004 1102 70-71 0.2698139751866009 1102 72-73 0.33296596565342185 1102 74-75 0.35494984396903106 1102 76-77 0.2997644167705005 1102 78-79 0.314960066135221 1102 80-81 0.3692298505101661 1102 82-83 0.3484162789822065 1102 84-85 0.25684911891328 1102 86-87 0.25097466690326087 1102 88-89 0.30527079069357654 1102 90-91 0.3093680236354359 1102 92-93 0.24645990073102908 1102 94-95 0.20523051212174437 1102 96-97 0.28303975040083884 1102 98-99 0.3252740388785398 1102 100 0.2929476265404283 1103 1 0.29769000512202837 1103 2 0.30051342923004043 1103 3 0.404865797065014 1103 4 0.2749834311108401 1103 5 0.2216855350065643 1103 6 0.25946203493898423 1103 7 0.30438637180511563 1103 8 0.2182799305179799 1103 9 0.34256185566304964 1103 10-11 0.29439218331128814 1103 12-13 0.29496819848406375 1103 14-15 0.3838361046126977 1103 16-17 0.38275886472363396 1103 18-19 0.3578212046694986 1103 20-21 0.36065413310564054 1103 22-23 0.3571241656291022 1103 24-25 0.39414572279758175 1103 26-27 0.3911532144295933 1103 28-29 0.40400214574751914 1103 30-31 0.4473931383485592 1103 32-33 0.45442612273516403 1103 34-35 0.471632030995778 1103 36-37 0.3858348676015666 1103 38-39 0.45158910095259586 1103 40-41 0.4590691269682807 1103 42-43 0.4136639451500699 1103 44-45 0.46685554579110544 1103 46-47 0.46177516231002613 1103 48-49 0.4737397235728267 1103 50-51 0.4436307843790672 1103 52-53 0.5098352408238469 1103 54-55 0.5074067709901868 1103 56-57 0.4613544998329644 1103 58-59 0.4505874583822518 1103 60-61 0.4383545966247411 1103 62-63 0.4905241269947638 1103 64-65 0.48535315785927935 1103 66-67 0.47375540409530714 1103 68-69 0.4800987248042574 1103 70-71 0.37412185408543763 1103 72-73 0.37775674532118586 1103 74-75 0.39326903951848635 1103 76-77 0.4166461913343724 1103 78-79 0.4144144275702857 1103 80-81 0.4269182515856329 1103 82-83 0.4667780544935525 1103 84-85 0.4738001872865141 1103 86-87 0.41700134943604183 1103 88-89 0.4147029789125867 1103 90-91 0.40431256650706615 1103 92-93 0.461364352757915 1103 94-95 0.4316662810562022 1103 96-97 0.4551108925804179 1103 98-99 0.4532101231680734 1103 100 0.4680244178114954 1104 1 0.21435129834591748 1104 2 0.22243012190186917 1104 3 0.3418669792224236 1104 4 0.21267827588155797 1104 5 0.1924189496024482 1104 6 0.16001403406084336 1104 7 0.17709198357588463 1104 8 0.18818577745589238 1104 9 0.24302891416001415 1104 10-11 0.2621446877091742 1104 12-13 0.22505970792723673 1104 14-15 0.3332002280605053 1104 16-17 0.3165408086915633 1104 18-19 0.28080172407164383 1104 20-21 0.27783662154411815 1104 22-23 0.278064730754366 1104 24-25 0.2931106885253598 1104 26-27 0.311776200466106 1104 28-29 0.3053059670986542 1104 30-31 0.311929220585796 1104 32-33 0.2868260287142803 1104 34-35 0.3154163944496986 1104 36-37 0.3584959401407417 1104 38-39 0.2493601279238291 1104 40-41 0.2376066195187292 1104 42-43 0.23294288085208592 1104 44-45 0.1846766406056446 1104 46-47 0.09524015935893715 1104 48-49 0.23446956928317775 1104 50-51 0.20280468795250073 1104 52-53 0.18800969553434044 1104 54-55 0.16763082343958047 1104 56-57 0.18876319584494894 1104 58-59 0.1669170349032285 1104 60-61 0.14003703017939273 1104 62-63 0.07067859479691663 1104 64-65 0.057799982327004784 1104 66-67 0.12200702374291694 1104 68-69 0.17449339067094627 1104 70-71 0.12610861989930555 1104 72-73 0.18188182527628527 1104 74-75 0.14438019277424274 1104 76-77 0.17762390505227899 1104 78-79 0.1777353071597645 1104 80-81 0.15982291636612445 1104 82-83 0.11384412600114047 1104 84-85 0.07593048436606864 1104 86-87 0.11155589677665034 1104 88-89 0.11670023299711829 1104 90-91 0.1210216172812153 1104 92-93 0.1478203459856502 1104 94-95 0.1692876217272996 1104 96-97 0.12119159536926105 1104 98-99 0.15866298945440604 1104 100 0.15028326373391465 1105 1 0.17617186231470683 1105 2 0.16618668757036303 1105 3 0.33384771599371277 1105 4 0.20282160909685842 1105 5 0.12149989372098702 1105 6 0.11004137871761799 1105 7 0.12442263359181993 1105 8 0.07920059865331552 1105 9 0.20513748228362516 1105 10-11 0.11029646432497486 1105 12-13 0.16675657175084524 1105 14-15 0.21026258568140577 1105 16-17 0.2574271690193797 1105 18-19 0.23770457775216158 1105 20-21 0.24229210877081897 1105 22-23 0.16025450660687568 1105 24-25 0.24100357616046608 1105 26-27 0.229017104925056 1105 28-29 0.26647812827081196 1105 30-31 0.2726650475944439 1105 32-33 0.28113637286350723 1105 34-35 0.1992787190209384 1105 36-37 0.21446868204258607 1105 38-39 0.2935113222361423 1105 40-41 0.342333900830333 1105 42-43 0.3134793869524941 1105 44-45 0.26813922170516946 1105 46-47 0.26990720373597554 1105 48-49 0.32482811871799555 1105 50-51 0.2664256713371742 1105 52-53 0.33633970422955173 1105 54-55 0.3444063572505627 1105 56-57 0.28466818594972665 1105 58-59 0.31302878208909135 1105 60-61 0.2896650248127557 1105 62-63 0.284142789034469 1105 64-65 0.1776695148710843 1105 66-67 0.17233417600347423 1105 68-69 0.17233493330303418 1105 70-71 0.1795483416526693 1105 72-73 0.19342166388378956 1105 74-75 0.15426400749156954 1105 76-77 0.12188487143999538 1105 78-79 0.12744592593590198 1105 80-81 0.1469811573374784 1105 82-83 0.11312057457941904 1105 84-85 0.15913929131278337 1105 86-87 0.19449283094881764 1105 88-89 0.17806557728405892 1105 90-91 0.16418328912172342 1105 92-93 0.17483883412121415 1105 94-95 0.1664949257015209 1105 96-97 0.23588846312735257 1105 98-99 0.24467668932417297 1105 100 0.18425752434619724 1106 1 0.17141545789198886 1106 2 0.15984340751253612 1106 3 0.201810462585307 1106 4 0.15832764308006375 1106 5 0.18987822149563982 1106 6 0.13434965550580102 1106 7 0.08561512935128235 1106 8 0.09217751000729635 1106 9 0.08687607838593436 1106 10-11 0.13084600932469215 1106 12-13 0.18020767901357004 1106 14-15 0.1998867791733332 1106 16-17 0.15119911828958266 1106 18-19 0.1821754312325723 1106 20-21 0.17398884118043156 1106 22-23 0.17019273057512407 1106 24-25 0.17869334480730714 1106 26-27 0.19307949988385786 1106 28-29 0.22200694143651134 1106 30-31 0.22228649647267673 1106 32-33 0.15023696740017556 1106 34-35 0.13450350232702135 1106 36-37 0.21618239883290613 1106 38-39 0.14967911925028687 1106 40-41 0.16567589580890996 1106 42-43 0.14378214451143378 1106 44-45 0.27183744976257174 1106 46-47 0.25943611357430285 1106 48-49 0.24445716250902905 1106 50-51 0.24306873099251902 1106 52-53 0.2545769399701996 1106 54-55 0.31984289805946986 1106 56-57 0.27999667565103437 1106 58-59 0.230916385157947 1106 60-61 0.2353356189438358 1106 62-63 0.24152771666056339 1106 64-65 0.24589199970208853 1106 66-67 0.23402759154187436 1106 68-69 0.3079650749395455 1106 70-71 0.3008971026040328 1106 72-73 0.33528024516297705 1106 74-75 0.3048487935533828 1106 76-77 0.3457538989189075 1106 78-79 0.2554213177517397 1106 80-81 0.2176079571012366 1106 82-83 0.2723721728724158 1106 84-85 0.23875581210934627 1106 86-87 0.31819618154432305 1106 88-89 0.2236858349777222 1106 90-91 0.23062216396345292 1106 92-93 0.2775019427906962 1106 94-95 0.24937937024226642 1106 96-97 0.3572510582651631 1106 98-99 0.39021316407345097 1106 100 0.3531169373433336 1107 1 0.12194406784922052 1107 2 0.15403057354667382 1107 3 0.18099453258025733 1107 4 0.10754205371543435 1107 5 0.12202021851974365 1107 6 0.09400459527845584 1107 7 0.05085990128545603 1107 8 0.08683133803278054 1107 9 0.08833803950794561 1107 10-11 0.14219278909056499 1107 12-13 0.18016581860661773 1107 14-15 0.2546809102355496 1107 16-17 0.20709559152312096 1107 18-19 0.19283537413932095 1107 20-21 0.23145442730495347 1107 22-23 0.18539697402438549 1107 24-25 0.2328183073639849 1107 26-27 0.21458275602551424 1107 28-29 0.23079441941222711 1107 30-31 0.22118502412799756 1107 32-33 0.16868609912290822 1107 34-35 0.18142390829685695 1107 36-37 0.20506655803945506 1107 38-39 0.24749574566029509 1107 40-41 0.2243943041131473 1107 42-43 0.24703225174474852 1107 44-45 0.2851005663405388 1107 46-47 0.37916090042599393 1107 48-49 0.34898798073864157 1107 50-51 0.3082174958115118 1107 52-53 0.29801809375174315 1107 54-55 0.36621335613735795 1107 56-57 0.29564164938499005 1107 58-59 0.2887248460703873 1107 60-61 0.2941354647670593 1107 62-63 0.2767908691188907 1107 64-65 0.24126522872146694 1107 66-67 0.28578260269468814 1107 68-69 0.2137826385877517 1107 70-71 0.27263050943771816 1107 72-73 0.28564353369480244 1107 74-75 0.28490491911720284 1107 76-77 0.2376509201432242 1107 78-79 0.2344462638360767 1107 80-81 0.2277921592011154 1107 82-83 0.28316313341654364 1107 84-85 0.22558435351336925 1107 86-87 0.225093526235419 1107 88-89 0.2618306143597877 1107 90-91 0.21687602178050014 1107 92-93 0.22633025912707083 1107 94-95 0.26304005195802205 1107 96-97 0.24473294820273495 1107 98-99 0.24233937374407333 1107 100 0.29545362875112957 1108 1 0.013857365517210951 1108 2 0.08404868807014765 1108 3 0.18088945584086247 1108 4 0.09049320263863336 1108 5 0.03608118513886183 1108 6 0.10628412794374498 1108 7 0.10308943366995038 1108 8 0.04768239392686269 1108 9 0.11536243472420438 1108 10-11 0.05669537823440152 1108 12-13 0.07138329634032203 1108 14-15 0.1594508855974368 1108 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-0.29069860422696436 2215 4 -0.281268624646863 2215 5 -0.2762335443139179 2215 6 -0.3203576380691828 2215 7 -0.3031361908791794 2215 8 -0.3263397631396927 2215 9 -0.4477796989706775 2215 10-11 -0.4507418264952108 2215 12-13 -0.4345687479069724 2215 14-15 -0.6043883782880854 2215 16-17 -0.6230103069788768 2215 18-19 -0.5500779483114684 2215 20-21 -0.5374689321749102 2215 22-23 -0.5470709824294318 2215 24-25 -0.5046250909154821 2215 26-27 -0.507114537979966 2215 28-29 -0.5032302784163818 2215 30-31 -0.5191430557487138 2215 32-33 -0.4745342453566792 2215 34-35 -0.5731808011429251 2215 36-37 -0.6044983306159466 2215 38-39 -0.538244136271139 2215 40-41 -0.6363997559439838 2215 42-43 -0.6609682607808693 2215 44-45 -0.6413640818804467 2215 46-47 -0.8011838800028599 2215 48-49 -0.7660842848219431 2215 50-51 -0.6700479299470032 2215 52-53 -0.7587705109572838 2215 54-55 -0.7333301524441325 2215 56-57 -0.6865336989606305 2215 58-59 -0.7369793695149482 2215 60-61 -0.7526311699254009 2215 62-63 -0.7857082425171669 2215 64-65 -0.8170060985335965 2215 66-67 -0.7551672663932507 2215 68-69 -0.7251969358724679 2215 70-71 -0.7552662918586961 2215 72-73 -0.6892129549913406 2215 74-75 -0.5962890992920435 2215 76-77 -0.653942579834073 2215 78-79 -0.7026265366058482 2215 80-81 -0.7560495011555872 2215 82-83 -0.8259168154744145 2215 84-85 -0.689748973748042 2215 86-87 -0.6787734547361381 2215 88-89 -0.6349127917717787 2215 90-91 -0.7410651204340439 2215 92-93 -0.6186203216179997 2215 94-95 -0.6243771887469975 2215 96-97 -0.5655975721736048 2215 98-99 -0.5974464142820111 2215 100 -0.6500550239481768 2216 1 -0.45833120162535934 2216 2 -0.473789587423763 2216 3 -0.3755840655567617 2216 4 -0.39407691263708955 2216 5 -0.41189296807066 2216 6 -0.41937848034557845 2216 7 -0.5186267590727383 2216 8 -0.45659655542569766 2216 9 -0.6306098008157051 2216 10-11 -0.6702527498861812 2216 12-13 -0.6026407739901245 2216 14-15 -0.8902096230343446 2216 16-17 -0.9179211510412202 2216 18-19 -0.8346329963539958 2216 20-21 -0.8465463772772424 2216 22-23 -0.783528650486474 2216 24-25 -0.8599222341289874 2216 26-27 -0.8938226040837165 2216 28-29 -0.9088434387744613 2216 30-31 -0.9353870941523894 2216 32-33 -0.9466714310750461 2216 34-35 -0.9776931971511829 2216 36-37 -1.0296793957772508 2216 38-39 -0.9669157254702441 2216 40-41 -1.0658248570811537 2216 42-43 -1.195528221341128 2216 44-45 -1.2901271061219575 2216 46-47 -1.2649663714710115 2216 48-49 -1.2040953099532459 2216 50-51 -1.180373378366923 2216 52-53 -1.1736798998522175 2216 54-55 -1.197423061924674 2216 56-57 -1.1501497717778761 2216 58-59 -1.1042361983698115 2216 60-61 -1.1896367790722877 2216 62-63 -1.147337640055099 2216 64-65 -1.2475520882727693 2216 66-67 -1.1968816091393464 2216 68-69 -1.2204430602184217 2216 70-71 -1.1709210178069682 2216 72-73 -1.1060188981742627 2216 74-75 -1.1511997620274812 2216 76-77 -1.0529775754723616 2216 78-79 -1.1039947695273256 2216 80-81 -1.0580497531924031 2216 82-83 -0.9496381003857266 2216 84-85 -1.1028148976875478 2216 86-87 -1.0479833033820327 2216 88-89 -1.1096105562310044 2216 90-91 -0.9838617192874786 2216 92-93 -1.0241897434977396 2216 94-95 -1.1051448145950786 2216 96-97 -1.1605182829993694 2216 98-99 -1.3399225235133372 2216 100 -1.3515723811820664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 204.0 3 27.0 4 27.0 5 90.0 6 276.0 7 782.0 8 1482.0 9 2334.0 10 3531.0 11 5107.0 12 7684.0 13 10949.0 14 11530.0 15 14141.0 16 17896.0 17 24745.0 18 24634.0 19 13068.0 20 9279.0 21 8782.0 22 9112.0 23 9765.0 24 11205.0 25 13752.0 26 17137.0 27 22249.0 28 28915.0 29 37551.0 30 49028.0 31 63819.0 32 83073.0 33 109677.0 34 150617.0 35 225451.0 36 374552.0 37 706225.0 38 940435.0 39 362448.0 40 968.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43252590533217 20.41668212847501 14.397264076306621 22.753527889886197 2 16.618389602872842 20.955971851541282 38.67130687874772 23.754331666838148 3 17.90421304729037 25.92384924509577 31.029397069929644 25.14254063768422 4 13.250905028158243 18.17282309186499 37.61717775912389 30.959094120852875 5 12.628230467734097 36.85368775004441 35.11358180579695 15.404499976424542 6 29.497414858757914 37.99358525599229 17.938985507375296 14.570014377874498 7 25.576752525613433 32.801974294205536 23.19730458908356 18.42396859109747 8 24.699670605035305 34.620540499509715 21.25126795860814 19.42852093684684 9 24.585306001665803 16.530266294287372 22.470405897975628 36.4140218060712 10-11 23.44797566942729 26.575463588795056 29.015222026557375 20.961338715220275 12-13 24.693236298856622 24.50936932828512 29.084205498099802 21.713188874758455 14-15 22.1333016263376 26.361678577051705 27.27371923949466 24.231300557116032 16-17 20.658703940968056 29.23183872604296 27.90444432649864 22.205013006490347 18-19 20.475222435743667 27.199146520419138 31.259223370348877 21.066407673488317 20-21 21.682648158795118 26.71615844049023 30.940354574747214 20.66083882596744 22-23 21.386254958048028 26.781717200679488 30.9446095191557 20.887418322116787 24-25 21.20200252212942 26.64575171228155 31.057995633567153 21.09425013202188 26-27 20.956920689318785 26.490987375416857 31.201299789150454 21.3507921461139 28-29 21.07580709772169 26.503366743295203 31.256421333787195 21.164404825195913 30-31 21.455104999277992 26.98760017280708 30.709357052696372 20.84793777521855 32-33 20.928855846931118 26.766432017107544 31.156333773851042 21.1483783621103 34-35 21.1720103530062 26.323576810048905 31.055653190303943 21.44875964664095 36-37 21.737147028640372 26.786327959254535 30.37028097755198 21.106244034553114 38-39 21.519240502800997 26.879832957109272 30.49468250553662 21.106244034553114 40-41 21.27528541485115 26.668983412240067 30.59488867019496 21.460842502713824 42-43 21.25002260902517 26.819730014140646 30.317664957671457 21.612582419162727 44-45 21.60883154482354 27.009616174363178 30.00211709429105 21.379435186522233 46-47 21.639357435196604 27.369878018008347 29.415231277725706 21.575533269069343 48-49 21.631855686518232 27.089259245312224 29.434445242720113 21.84443982544943 50-51 21.689660662994466 27.0786589482667 29.203996267509392 22.027684121229445 52-53 21.672240594423148 27.260420685019366 29.02260517051356 22.04473355004393 54-55 21.798020902303215 27.66016307556277 28.846239948620433 21.69557607351358 56-57 21.4931474639197 27.382153606754777 28.904682425478427 22.22001650384709 58-59 21.38216309513255 26.894006221410706 29.32948006358399 22.394350619872753 60-61 21.776360714913682 27.334830322601878 28.884371366803784 22.004437595680653 62-63 21.579521352852904 27.296254136710324 28.993428408855383 22.13079610158139 64-65 21.54257598189143 26.974346095102604 29.275989333877334 22.20708858912863 66-67 21.464711981775196 27.100052275031306 28.9855411948299 22.449694548363595 68-69 21.932978250562556 27.289493667545628 28.73181604288984 22.045712039001977 70-71 21.9660837936432 26.961773994550708 28.856825420075687 22.215316791730405 72-73 21.64863825470779 27.16406917383212 29.043627857521333 22.143664713938755 74-75 21.677373462456337 27.145985978251154 28.82757880432486 22.349061754967646 76-77 21.754893260197708 27.28428988535964 28.525977547533067 22.434839306909584 78-79 21.87115553912221 27.36683877200229 28.6050127692809 22.156992919594597 80-81 21.606747913070834 27.13756608893181 28.7470508124591 22.508635185538257 82-83 21.506789565572177 27.224577527599475 28.809901652410296 22.45873125441805 84-85 21.569421349394048 27.23861213045613 28.552578830885718 22.639387689264105 86-87 21.704159971036628 27.327703444485614 28.465956844635144 22.50217973984262 88-89 21.45421028684239 27.22622457343986 28.521497871850865 22.798067267866884 90-91 21.30677298869703 27.07459389065422 28.513042837532687 23.105590283116058 92-93 21.398123847864266 27.284768369013157 28.307483882330857 23.009623900791716 94-95 21.32142656996795 27.037850140710095 28.51289696747582 23.127826321846136 96-97 21.20465401552574 27.12679818717618 28.464503709901933 23.20404408739615 98-99 21.13582515803889 27.205446628229403 28.38876462431451 23.269963589417195 100 21.225490312357675 27.15712530362836 28.283663608243508 23.333720775770455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 867.0 1 741.5 2 2005.0 3 2994.0 4 1678.5 5 823.0 6 1499.5 7 3524.0 8 5653.5 9 6338.0 10 4889.5 11 3044.5 12 2683.0 13 2824.5 14 3155.0 15 3572.5 16 3935.0 17 4426.0 18 4959.0 19 5381.0 20 6038.0 21 7117.5 22 8808.0 23 11460.5 24 14781.0 25 19126.0 26 24807.5 27 31153.5 28 39411.5 29 48905.0 30 57159.0 31 64494.0 32 71965.5 33 78822.5 34 84739.0 35 90759.5 36 95784.0 37 100722.0 38 105578.5 39 108293.0 40 111194.5 41 113594.0 42 113232.0 43 112769.5 44 112074.0 45 111271.0 46 111119.5 47 109139.5 48 106858.0 49 106239.0 50 104972.0 51 100927.0 52 94507.5 53 89944.0 54 87474.5 55 84268.5 56 79015.5 57 72230.5 58 66619.0 59 61690.0 60 55036.5 61 47183.5 62 39837.0 63 32943.5 64 26589.5 65 21049.5 66 16570.0 67 12852.0 68 9812.0 69 7453.0 70 5498.0 71 4046.5 72 3020.0 73 2157.0 74 1568.0 75 1123.5 76 760.0 77 545.0 78 372.5 79 231.0 80 130.0 81 64.0 82 33.5 83 19.5 84 8.0 85 3.0 86 2.5 87 2.5 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11955355996521323 2 0.0 3 0.0 4 0.0 5 0.011741867496583442 6 1.1860472218771153E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.4825590273463942E-5 76-77 0.0 78-79 0.0 80-81 2.3720944437542307E-4 82-83 5.633724303916298E-4 84-85 3.2616298601620674E-4 86-87 3.409885762896707E-4 88-89 4.595932984773822E-4 90-91 7.561051039466611E-4 92-93 1.7790708328156731E-4 94-95 2.9651180546927885E-4 96-97 5.485468401181659E-4 98-99 0.0011415704510567236 100 8.895354164078366E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3372547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.02565858213484 #Duplication Level Percentage of deduplicated Percentage of total 1 79.57374064807897 50.94761150837249 2 12.761043485900704 16.340684267601084 3 3.4381642441690117 6.603921901394065 4 1.4122121804998742 3.6167125965686853 5 0.7366631640893061 2.358267211700854 6 0.46712495594877484 1.794478976686262 7 0.3113419527418663 1.3953711497982124 8 0.2206602463064716 1.1302334074134346 9 0.1538638279003874 0.8866109621961502 >10 0.8647954808571057 9.720049575248645 >50 0.04493206424320572 1.9419732907147258 >100 0.014440158889669803 1.540847176758732 >500 5.550492964962123E-4 0.24932445246584173 >1k 3.2377875469027516E-4 0.48926110739032946 >5k 4.6254107812896436E-5 0.21852537445876402 >10k+ 9.250821562579287E-5 0.7661270412316337 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13107 0.38863802342858383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12763 0.3784380173204406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7379 0.21879606125578088 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 0.12785589051835305 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3619 0.10730762239933203 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9651180546927887E-5 0.0 4 0.0 0.0 0.0 5.9302361093855774E-5 0.0 5 0.0 0.0 0.0 5.9302361093855774E-5 0.0 6 0.0 0.0 0.0 5.9302361093855774E-5 0.0 7 0.0 0.0 0.0 8.895354164078366E-5 0.0 8 0.0 0.0 0.0 8.895354164078366E-5 0.0 9 2.9651180546927887E-5 0.0 0.0 1.4825590273463942E-4 0.0 10-11 2.9651180546927887E-5 0.0 0.0 1.6308149300810337E-4 0.0 12-13 2.9651180546927887E-5 1.4825590273463943E-5 0.0 2.52035034648887E-4 0.0 14-15 2.9651180546927887E-5 7.412795136731971E-5 0.0 2.816862151958149E-4 0.0 16-17 2.9651180546927887E-5 8.895354164078366E-5 0.0 3.409885762896707E-4 0.0 18-19 2.9651180546927887E-5 8.895354164078366E-5 0.0 3.5581416656313463E-4 0.0 20-21 2.9651180546927887E-5 8.895354164078366E-5 0.0 3.706397568365986E-4 0.0 22-23 2.9651180546927887E-5 8.895354164078366E-5 0.0 6.226747914854856E-4 0.0 24-25 2.9651180546927887E-5 8.895354164078366E-5 0.0 0.0014825590273463943 0.0 26-27 2.9651180546927887E-5 8.895354164078366E-5 0.0 0.0022683153118399834 0.0 28-29 2.9651180546927887E-5 1.1860472218771155E-4 0.0 0.005011049512430813 0.0 30-31 2.9651180546927887E-5 1.1860472218771155E-4 0.0 0.012364542288068929 0.0 32-33 2.9651180546927887E-5 1.1860472218771155E-4 0.0 0.028895075442981224 0.0 34-35 2.9651180546927887E-5 1.1860472218771155E-4 0.0 0.05753811585131356 0.0 36-37 2.9651180546927887E-5 1.3343031246117548E-4 0.0 0.10174802604678304 0.0 38-39 2.9651180546927887E-5 1.6308149300810337E-4 0.0 0.15900445568290078 0.0 40-41 2.9651180546927887E-5 1.7790708328156731E-4 0.0 0.2244149599694237 0.0 42-43 2.9651180546927887E-5 2.075582638284952E-4 0.0 0.29234581460243547 0.0 44-45 2.9651180546927887E-5 2.2238385410195915E-4 0.0 0.37980197162559926 0.0 46-47 2.9651180546927887E-5 2.372094443754231E-4 0.0 0.48368488267176113 0.0 48-49 4.447677082039183E-5 2.372094443754231E-4 0.0 0.5895099460437467 0.0 50-51 5.9302361093855774E-5 2.372094443754231E-4 0.0 0.6966841381306176 0.0 52-53 5.9302361093855774E-5 2.372094443754231E-4 0.0 0.8032653066065498 0.0 54-55 5.9302361093855774E-5 2.372094443754231E-4 0.0 0.9330336982701798 0.0 56-57 5.9302361093855774E-5 2.372094443754231E-4 0.0 1.0849811729829117 0.0 58-59 5.9302361093855774E-5 2.372094443754231E-4 0.0 1.2550455190098164 0.0 60-61 5.9302361093855774E-5 2.372094443754231E-4 0.0 1.4296168444798547 0.0 62-63 8.895354164078366E-5 2.372094443754231E-4 0.0 1.6068864273796628 0.0 64-65 8.895354164078366E-5 2.372094443754231E-4 0.0 1.806008930342557 1.4825590273463943E-5 66-67 8.895354164078366E-5 2.9651180546927885E-4 0.0 2.0358945331228893 2.9651180546927887E-5 68-69 8.895354164078366E-5 2.9651180546927885E-4 0.0 2.287010381174821 2.9651180546927887E-5 70-71 8.895354164078366E-5 3.113373957427428E-4 0.0 2.5615654874491 2.9651180546927887E-5 72-73 8.895354164078366E-5 4.151165276569904E-4 0.0 2.856490954759118 2.9651180546927887E-5 74-75 1.4825590273463942E-4 4.151165276569904E-4 0.0 3.1588588683864156 2.9651180546927887E-5 76-77 1.4825590273463942E-4 4.151165276569904E-4 0.0 3.4795956883625343 2.9651180546927887E-5 78-79 1.4825590273463942E-4 4.151165276569904E-4 0.0 3.839976136729896 2.9651180546927887E-5 80-81 1.4825590273463942E-4 4.151165276569904E-4 0.0 4.2273095082144145 2.9651180546927887E-5 82-83 1.4825590273463942E-4 4.151165276569904E-4 0.0 4.630951028999744 2.9651180546927887E-5 84-85 1.4825590273463942E-4 4.151165276569904E-4 0.0 5.039055052457385 2.9651180546927887E-5 86-87 1.4825590273463942E-4 4.151165276569904E-4 0.0 5.474364034066834 2.9651180546927887E-5 88 1.4825590273463942E-4 4.151165276569904E-4 0.0 5.825567442054922 2.9651180546927887E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7105 0.0 58.503098 1 GTATCAA 12690 0.0 39.246983 1 GTGGTAT 2790 0.0 37.11938 1 TATAACG 330 0.0 34.18648 2 TGGTATC 3095 0.0 32.350132 2 ATCAACG 15600 0.0 31.638931 3 TCAACGC 15830 0.0 31.208935 4 TATCAAC 16235 0.0 30.430393 2 CAACGCA 16420 0.0 30.173422 5 AACGCAG 17055 0.0 29.238153 6 ATAACGC 430 0.0 27.32931 3 ACGCAGA 19195 0.0 25.905014 7 CGCAGAG 19410 0.0 25.642284 8 GCAGAGT 20925 0.0 23.471298 9 CAGAGTA 20405 0.0 21.754627 10-11 TAACGCA 620 0.0 21.228703 4 GAGTACT 16520 0.0 21.010035 12-13 AGAGTAC 19800 0.0 20.47294 10-11 GTACATG 8555 0.0 20.084225 1 CTGTCGC 930 0.0 19.203789 9 >>END_MODULE