FastQCFastQC Report
Wed 25 May 2016
SRR2049459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012212
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74250.045.415291
GTATCAA134100.034.8958441
TCAACGC135450.033.554674
ATCAACG137300.032.999853
CAACGCA156700.029.2142985
TATCAAC161200.028.4568962
AACGCAG164500.027.8011826
ACGCAGA171550.026.0289927
CGCAGAG170450.025.8088138
GTACATG188650.021.8386941
GCAGAGT202900.020.7550269
TACATGG188100.020.439362
ACATGGG188100.019.4649873
TGGTATC42950.018.1655122
GTGGTAT48450.018.1521151
AGAGTAC181150.017.88968810-11
CAGAGTA202800.017.4747210-11
GAGTACT101350.016.25349212-13
CATGGGG113100.015.7916124
GTATAGG29500.014.8265571
Produced by FastQC (version 0.11.5)