FastQCFastQC Report
Wed 25 May 2016
SRR2049459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012212
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201880.2519653748552834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197710.24676081960886706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104720.13070048570856588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139300.059.59211
GTATCAA261100.040.246031
ATCAACG319250.032.6414073
TCAACGC323300.032.2325024
GTGGTAT57400.031.8098071
TGGTATC55350.031.1661032
TATCAAC336150.031.1262022
CAACGCA342350.030.466395
AACGCAG355000.029.4570756
CGCAGAG388450.026.6785018
ACGCAGA390050.026.6052157
GCAGAGT431600.023.9350439
GAGTACT339350.021.8063412-13
CAGAGTA428150.021.31225410-11
GTACATG177850.021.1414181
AGAGTAC405950.020.87426210-11
TACATGG175750.020.2457832
GTACTTT376300.019.65886514-15
AGTACTT355500.019.46059812-13
ACATGGG174850.019.3822273
Produced by FastQC (version 0.11.5)