##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049459_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8012212 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.876328035254186 33.0 31.0 34.0 30.0 34.0 2 31.97519099594469 33.0 31.0 34.0 30.0 34.0 3 32.004666876013765 33.0 31.0 34.0 30.0 34.0 4 35.744199853922986 37.0 35.0 37.0 33.0 37.0 5 35.821364686805595 37.0 35.0 37.0 35.0 37.0 6 35.91108472916093 37.0 35.0 37.0 35.0 37.0 7 35.902590320875184 37.0 35.0 37.0 35.0 37.0 8 35.92832341430806 37.0 35.0 37.0 35.0 37.0 9 37.639482829460825 39.0 37.0 39.0 35.0 39.0 10-11 37.62493310211961 39.0 37.0 39.0 35.0 39.0 12-13 37.612973171453774 39.0 37.0 39.0 35.0 39.0 14-15 38.91407965740297 40.0 38.0 41.0 35.0 41.0 16-17 38.93566402137138 40.0 38.0 41.0 35.5 41.0 18-19 39.02247737578586 40.0 38.5 41.0 36.0 41.0 20-21 39.053489223700026 40.0 39.0 41.0 36.0 41.0 22-23 39.025316853822645 40.0 39.0 41.0 35.0 41.0 24-25 39.00847163055596 40.0 39.0 41.0 35.0 41.0 26-27 38.91079086524421 40.0 38.0 41.0 35.0 41.0 28-29 38.829493840153006 40.0 38.0 41.0 35.0 41.0 30-31 38.71361841149485 40.0 38.0 41.0 35.0 41.0 32-33 38.57066700182172 40.0 38.0 41.0 35.0 41.0 34-35 38.45243385971315 40.0 38.0 41.0 34.0 41.0 36-37 38.308803486477885 40.0 38.0 41.0 34.0 41.0 38-39 38.19427631220941 40.0 38.0 41.0 34.0 41.0 40-41 38.095962263604605 40.0 38.0 41.0 34.0 41.0 42-43 37.945647718757314 40.0 38.0 41.0 33.0 41.0 44-45 37.765393314605255 40.0 38.0 41.0 33.0 41.0 46-47 37.51436912802607 40.0 38.0 41.0 33.0 41.0 48-49 37.26318137862553 40.0 37.0 41.0 32.0 41.0 50-51 37.10528653011178 40.0 37.0 41.0 32.0 41.0 52-53 36.99600029305265 40.0 37.0 41.0 31.5 41.0 54-55 36.8661173718319 40.0 37.0 41.0 31.0 41.0 56-57 36.73614433816779 40.0 36.5 41.0 31.0 41.0 58-59 36.57697643297506 40.0 36.0 41.0 31.0 41.0 60-61 36.41047241386024 39.0 36.0 41.0 31.0 41.0 62-63 36.21416258082037 39.0 35.0 41.0 30.5 41.0 64-65 36.014453936066595 39.0 35.0 41.0 30.0 41.0 66-67 35.77238745055672 38.5 35.0 40.5 30.0 41.0 68-69 35.49352656919213 38.0 35.0 40.0 29.5 41.0 70-71 35.215453859683194 37.0 35.0 40.0 29.0 41.0 72-73 34.88839361714343 37.0 35.0 39.5 29.0 41.0 74-75 34.55983653453004 36.0 34.0 39.0 29.0 41.0 76-77 34.23009482275307 36.0 34.0 39.0 29.0 40.5 78-79 33.90365463370165 35.5 34.0 38.5 29.0 39.5 80-81 33.571166489354 35.0 34.0 37.0 29.0 39.0 82-83 33.27510405366208 35.0 34.0 37.0 28.5 39.0 84-85 32.967089799920416 35.0 34.0 36.5 28.0 38.5 86-87 32.69883529042916 35.0 34.0 36.0 27.5 37.0 88-89 32.45721893030289 35.0 34.0 36.0 27.0 37.0 90-91 32.24427686137112 35.0 34.0 35.0 27.0 36.5 92-93 32.067089774958525 35.0 33.0 35.0 26.5 36.0 94-95 31.89440256947769 35.0 33.0 35.0 26.0 36.0 96-97 31.74271262417919 35.0 33.0 35.0 26.0 36.0 98-99 31.600806181863387 35.0 33.0 35.0 25.5 36.0 100 31.486227523685095 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.298894973300051 1101 2 0.3323719855424869 1101 3 0.45151828468810606 1101 4 0.2526116177514126 1101 5 0.2539583909380312 1101 6 0.2563395359900156 1101 7 0.2831009484907341 1101 8 0.28361195665224415 1101 9 0.35571602797777757 1101 10-11 0.3450581380900175 1101 12-13 0.3156828856551641 1101 14-15 0.46329484285170963 1101 16-17 0.4341450101683151 1101 18-19 0.4535119250372972 1101 20-21 0.47780816411031424 1101 22-23 0.4440399663638175 1101 24-25 0.4368560154042953 1101 26-27 0.47651655963740325 1101 28-29 0.47615641171886836 1101 30-31 0.467096078397347 1101 32-33 0.491072138740428 1101 34-35 0.5134502349543979 1101 36-37 0.5861718341883986 1101 38-39 0.5400869370368397 1101 40-41 0.5645184447062732 1101 42-43 0.509155597482696 1101 44-45 0.5108096214179838 1101 46-47 0.5482962763384123 1101 48-49 0.5788790821044358 1101 50-51 0.500212991368322 1101 52-53 0.5125074819789006 1101 54-55 0.49706708248599796 1101 56-57 0.5522460251263297 1101 58-59 0.5380732905411278 1101 60-61 0.44763554168726927 1101 62-63 0.45317164473193117 1101 64-65 0.4741125598804743 1101 66-67 0.5411432749974594 1101 68-69 0.5075791369876441 1101 70-71 0.5384238367159924 1101 72-73 0.48737215073899876 1101 74-75 0.47231344838953504 1101 76-77 0.4756643050191869 1101 78-79 0.4605031044165031 1101 80-81 0.46855131084595314 1101 82-83 0.4143079721159424 1101 84-85 0.4111002575506362 1101 86-87 0.34322982869714025 1101 88-89 0.43175596968607977 1101 90-91 0.41998043785046235 1101 92-93 0.44405777962116844 1101 94-95 0.41401125559793783 1101 96-97 0.3802296939912715 1101 98-99 0.4362474741467217 1101 100 0.4358655753858436 1102 1 0.21934789817196076 1102 2 0.2716457476127019 1102 3 0.32651852535322234 1102 4 0.24131228741929078 1102 5 0.23698178771297762 1102 6 0.21341294160081503 1102 7 0.22428435387461576 1102 8 0.2152143978216543 1102 9 0.2521139416175089 1102 10-11 0.29087665905847615 1102 12-13 0.26391589658892656 1102 14-15 0.37904139807438497 1102 16-17 0.3660027282641991 1102 18-19 0.36820348174865813 1102 20-21 0.37537617435302195 1102 22-23 0.3223693189815293 1102 24-25 0.29894491641048404 1102 26-27 0.30562994763150897 1102 28-29 0.3308824901220575 1102 30-31 0.31839163028715234 1102 32-33 0.3615019339756742 1102 34-35 0.36126374080564716 1102 36-37 0.3854463491966129 1102 38-39 0.3499763823587543 1102 40-41 0.40813494003083406 1102 42-43 0.34112969435247464 1102 44-45 0.38431851193116984 1102 46-47 0.3793268026455152 1102 48-49 0.3830562549494303 1102 50-51 0.34896372453740554 1102 52-53 0.30299253736281884 1102 54-55 0.3246195251337838 1102 56-57 0.29306345622562446 1102 58-59 0.35422554698286746 1102 60-61 0.2885173421098841 1102 62-63 0.25784653022691373 1102 64-65 0.225416715548171 1102 66-67 0.2589830408509357 1102 68-69 0.2910159104798282 1102 70-71 0.2552552071388021 1102 72-73 0.23147380115159422 1102 74-75 0.22516776244925296 1102 76-77 0.24375660593584314 1102 78-79 0.23857769581870514 1102 80-81 0.26686501335498747 1102 82-83 0.20916143456496883 1102 84-85 0.1322248271898374 1102 86-87 0.1589757134955576 1102 88-89 0.19799517499610886 1102 90-91 0.22344536228505518 1102 92-93 0.23306360406078142 1102 94-95 0.16800448293777137 1102 96-97 0.16757363125881497 1102 98-99 0.22412901430719856 1102 100 0.2609473843348695 1103 1 0.3065880805993828 1103 2 0.28283784953013935 1103 3 0.39673105199978664 1103 4 0.23688442860841263 1103 5 0.2645744542375894 1103 6 0.24453162788635296 1103 7 0.25062264158704295 1103 8 0.24924522495413726 1103 9 0.34489640503596064 1103 10-11 0.31059118010956155 1103 12-13 0.2969336364624482 1103 14-15 0.3961389666829689 1103 16-17 0.3790615080031401 1103 18-19 0.357856890777704 1103 20-21 0.37590692408655 1103 22-23 0.3762160266443999 1103 24-25 0.3694580342442748 1103 26-27 0.3880650669638399 1103 28-29 0.3923809912182108 1103 30-31 0.4078651553008541 1103 32-33 0.4192754946722985 1103 34-35 0.4559927245332105 1103 36-37 0.39852640034623477 1103 38-39 0.4283028286448243 1103 40-41 0.45501928617992604 1103 42-43 0.4928251244250319 1103 44-45 0.4673569993091604 1103 46-47 0.49987645475132325 1103 48-49 0.5088893292629848 1103 50-51 0.51910669035572 1103 52-53 0.5266927608497483 1103 54-55 0.5321101437335827 1103 56-57 0.5377724496569058 1103 58-59 0.5244070485586363 1103 60-61 0.5023937470687585 1103 62-63 0.5851569832117747 1103 64-65 0.5602363489660789 1103 66-67 0.5256140259575091 1103 68-69 0.5183236457012299 1103 70-71 0.5138665634443811 1103 72-73 0.5278433644274756 1103 74-75 0.5385042042927637 1103 76-77 0.5178784552232472 1103 78-79 0.5344115813682819 1103 80-81 0.46045885195602665 1103 82-83 0.4512905951272401 1103 84-85 0.45896423468629166 1103 86-87 0.44491240499674234 1103 88-89 0.4355993576340751 1103 90-91 0.43496109089083035 1103 92-93 0.4666113814828279 1103 94-95 0.452069953368305 1103 96-97 0.4666217427938655 1103 98-99 0.5555240488387518 1103 100 0.5162346171416843 1104 1 0.21470674286513258 1104 2 0.1950839366173227 1104 3 0.30361457063294495 1104 4 0.1651533085830934 1104 5 0.1923265086930641 1104 6 0.13583901178245128 1104 7 0.13748938069112882 1104 8 0.150336734812484 1104 9 0.23694142997471346 1104 10-11 0.2029205505928715 1104 12-13 0.19299260721484046 1104 14-15 0.3086149377171168 1104 16-17 0.2693259525015179 1104 18-19 0.22611066509170286 1104 20-21 0.25241603111664546 1104 22-23 0.2254625894998057 1104 24-25 0.2542560613260676 1104 26-27 0.2113681897612949 1104 28-29 0.23630093333739666 1104 30-31 0.2353915988473858 1104 32-33 0.24002054833623276 1104 34-35 0.2400691423197614 1104 36-37 0.21785233016991157 1104 38-39 0.18773123597437547 1104 40-41 0.24922692393689516 1104 42-43 0.2526598277619101 1104 44-45 0.2864652437668411 1104 46-47 0.2612294651105387 1104 48-49 0.21139243801597019 1104 50-51 0.25892461023610025 1104 52-53 0.26218089142624734 1104 54-55 0.2014326527703858 1104 56-57 0.21893508360466996 1104 58-59 0.24790772474334943 1104 60-61 0.20774824466513309 1104 62-63 0.20615164434925504 1104 64-65 0.19884216741427707 1104 66-67 0.2350689826776815 1104 68-69 0.26227463803968476 1104 70-71 0.23939521247550033 1104 72-73 0.23464002806452555 1104 74-75 0.22605001082829546 1104 76-77 0.21846600973295693 1104 78-79 0.19035323680447647 1104 80-81 0.20729410175476914 1104 82-83 0.13494672349244752 1104 84-85 0.14524093322087595 1104 86-87 0.15798197484712517 1104 88-89 0.19125601950064208 1104 90-91 0.17750431750111062 1104 92-93 0.16836643759041436 1104 94-95 0.16388958105001805 1104 96-97 0.1857574281675589 1104 98-99 0.23085889910058555 1104 100 0.20751670056819194 1105 1 0.19319973856046602 1105 2 0.1837908891425073 1105 3 0.28731820233640093 1105 4 0.20047256387829293 1105 5 0.14208610669493993 1105 6 0.14428591812951197 1105 7 0.13874387203328098 1105 8 0.07786236713252492 1105 9 0.15526475531576978 1105 10-11 0.15392576215460707 1105 12-13 0.1726788982572245 1105 14-15 0.2113033161006399 1105 16-17 0.2207965761095778 1105 18-19 0.21977930770989929 1105 20-21 0.21387367798683954 1105 22-23 0.1873659807140058 1105 24-25 0.20668424860356538 1105 26-27 0.18959952610199338 1105 28-29 0.2208058645660671 1105 30-31 0.2336238844278853 1105 32-33 0.21859172542469452 1105 34-35 0.20321680404468623 1105 36-37 0.22479678947388493 1105 38-39 0.23064657979456626 1105 40-41 0.27940340893757565 1105 42-43 0.25992707033106655 1105 44-45 0.2705206215517606 1105 46-47 0.2439545982715785 1105 48-49 0.270371791253595 1105 50-51 0.2542799442315484 1105 52-53 0.23711489801416974 1105 54-55 0.2813288616310743 1105 56-57 0.27540362169266785 1105 58-59 0.29380400401925755 1105 60-61 0.25500376552651716 1105 62-63 0.29394163578029975 1105 64-65 0.26651936472420346 1105 66-67 0.25171112541922014 1105 68-69 0.2694604288531792 1105 70-71 0.29054411891102916 1105 72-73 0.31393864456459397 1105 74-75 0.32609974917273377 1105 76-77 0.2645433805921513 1105 78-79 0.2201844823034662 1105 80-81 0.2876200066606671 1105 82-83 0.23180151112489256 1105 84-85 0.220005229997696 1105 86-87 0.2303293333312979 1105 88-89 0.23032702939087102 1105 90-91 0.21746515434060143 1105 92-93 0.26761942153065377 1105 94-95 0.24268975939480342 1105 96-97 0.27184767332865434 1105 98-99 0.2953511199248773 1105 100 0.25661752918396985 1106 1 0.17464977575939145 1106 2 0.1195936132342581 1106 3 0.26380654698250083 1106 4 0.13565315398303568 1106 5 0.15036273356907515 1106 6 0.13903862993991822 1106 7 0.12241857948845336 1106 8 0.11119779386615392 1106 9 0.1441908371191971 1106 10-11 0.1507153115635802 1106 12-13 0.1659603933575724 1106 14-15 0.20513866351706156 1106 16-17 0.2082376694692485 1106 18-19 0.22885009004460954 1106 20-21 0.2006354964586734 1106 22-23 0.1699908647365902 1106 24-25 0.18698369464891584 1106 26-27 0.17648817516474224 1106 28-29 0.17844792007168309 1106 30-31 0.23340547090069208 1106 32-33 0.22839648491699194 1106 34-35 0.18638417099161586 1106 36-37 0.2024642122589384 1106 38-39 0.17223433200789628 1106 40-41 0.19210379755158868 1106 42-43 0.20307384811879814 1106 44-45 0.2346713725880818 1106 46-47 0.2113613246417927 1106 48-49 0.24044050120910754 1106 50-51 0.24247964056826277 1106 52-53 0.2453858057177385 1106 54-55 0.2764635857297506 1106 56-57 0.24572158732679128 1106 58-59 0.20667122838681706 1106 60-61 0.188724051069606 1106 62-63 0.20453104805547184 1106 64-65 0.22777193696290254 1106 66-67 0.2376667186580761 1106 68-69 0.19738272378911148 1106 70-71 0.24328239209472713 1106 72-73 0.283711539857741 1106 74-75 0.30016354653491817 1106 76-77 0.25219113342225086 1106 78-79 0.195651294540923 1106 80-81 0.17827565636616782 1106 82-83 0.18908810348680305 1106 84-85 0.19416146672017476 1106 86-87 0.1677823865072341 1106 88-89 0.149456244738019 1106 90-91 0.1990125444511861 1106 92-93 0.18827722311036865 1106 94-95 0.1258577193064916 1106 96-97 0.12749340589804703 1106 98-99 0.16935559685965984 1106 100 0.15101138989532714 1107 1 0.08774545725946581 1107 2 0.07736321923599832 1107 3 0.19473037673469662 1107 4 0.09598791765501602 1107 5 0.12471401366968848 1107 6 0.09083924984242486 1107 7 0.09481271901058363 1107 8 0.07426574970860855 1107 9 0.0569361167096929 1107 10-11 0.06102016890825013 1107 12-13 0.07650720934651645 1107 14-15 0.12951733400255705 1107 16-17 0.1463103065571687 1107 18-19 0.0923352704452185 1107 20-21 0.10708194095705181 1107 22-23 0.15153797068056463 1107 24-25 0.12299399584759385 1107 26-27 0.1458048288568179 1107 28-29 0.15037968357800935 1107 30-31 0.1221655560789614 1107 32-33 0.11349517537803422 1107 34-35 0.084525864622357 1107 36-37 0.1082879784685673 1107 38-39 0.11551582207277988 1107 40-41 0.14786603143689803 1107 42-43 0.09300789773810436 1107 44-45 0.18265180772012002 1107 46-47 0.1500915223518362 1107 48-49 0.1080065898199507 1107 50-51 0.11159972525704376 1107 52-53 0.14857391618127025 1107 54-55 0.17944985851875828 1107 56-57 0.07348558547307249 1107 58-59 0.06495714949988951 1107 60-61 0.12584734596681102 1107 62-63 0.10508452954563552 1107 64-65 0.018229290034447843 1107 66-67 0.057700096036001014 1107 68-69 0.07842586103996041 1107 70-71 0.09585848244982742 1107 72-73 0.09384510636581211 1107 74-75 0.11479372451136527 1107 76-77 0.12314895619692123 1107 78-79 0.11280355602391978 1107 80-81 0.09074569101689178 1107 82-83 0.11099794085522063 1107 84-85 0.07123238000336585 1107 86-87 0.09520864767122106 1107 88-89 0.09341634077352978 1107 90-91 0.11677822580625019 1107 92-93 0.13103948747755823 1107 94-95 0.13874152380929772 1107 96-97 0.0727972750263497 1107 98-99 0.07368793377176175 1107 100 0.06216018457450545 1108 1 0.04156046153942938 1108 2 0.06068179641043514 1108 3 0.13370020916185155 1108 4 0.05977080926919598 1108 5 0.02532532665230036 1108 6 0.04504769275894205 1108 7 0.02280009317862408 1108 8 0.03813275822855644 1108 9 0.08792477119552444 1108 10-11 0.02469084571409752 1108 12-13 0.02632604859577725 1108 14-15 0.06616465582092701 1108 16-17 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-0.39189556911505363 2215 3 -0.28824714749659464 2215 4 -0.26464066558513366 2215 5 -0.3045023308430075 2215 6 -0.2887435003743022 2215 7 -0.28589112555054896 2215 8 -0.2750840358823723 2215 9 -0.45363059115275206 2215 10-11 -0.4364881354986281 2215 12-13 -0.4373248055864991 2215 14-15 -0.6177509418441502 2215 16-17 -0.5993688116654639 2215 18-19 -0.5155134265135857 2215 20-21 -0.5383898700038685 2215 22-23 -0.5498219335797501 2215 24-25 -0.49689037722507123 2215 26-27 -0.5161424220101622 2215 28-29 -0.5161423799761096 2215 30-31 -0.5477019193939583 2215 32-33 -0.4949857224667582 2215 34-35 -0.5630249545937289 2215 36-37 -0.5968322545604892 2215 38-39 -0.5206789661639704 2215 40-41 -0.5454134035025149 2215 42-43 -0.5785300074908761 2215 44-45 -0.5687444562621309 2215 46-47 -0.5654159019697644 2215 48-49 -0.5208027473575711 2215 50-51 -0.5666845256617208 2215 52-53 -0.5763282747776657 2215 54-55 -0.5340493533078927 2215 56-57 -0.4680005023954763 2215 58-59 -0.5019988716369141 2215 60-61 -0.5110677438979323 2215 62-63 -0.4633516657667158 2215 64-65 -0.530183267081334 2215 66-67 -0.44745951414351737 2215 68-69 -0.38515691079486203 2215 70-71 -0.4059689002809961 2215 72-73 -0.37810724763232173 2215 74-75 -0.38492958838188684 2215 76-77 -0.4391197144251535 2215 78-79 -0.4340234387017716 2215 80-81 -0.4810248445277665 2215 82-83 -0.45236542598895824 2215 84-85 -0.44050067779497226 2215 86-87 -0.412900466793225 2215 88-89 -0.3661091044483413 2215 90-91 -0.35482674454403806 2215 92-93 -0.308984649849549 2215 94-95 -0.36543028999512117 2215 96-97 -0.29932405146744756 2215 98-99 -0.34490742418350706 2215 100 -0.3641048463634178 2216 1 -0.2969982507478015 2216 2 -0.25635550048332334 2216 3 -0.26284919813215524 2216 4 -0.24873126247903343 2216 5 -0.2562612448314425 2216 6 -0.2083752146561011 2216 7 -0.3099573680171801 2216 8 -0.28995567486987994 2216 9 -0.4247213847878797 2216 10-11 -0.42077941736710045 2216 12-13 -0.44631301947838864 2216 14-15 -0.639170452626459 2216 16-17 -0.6303296582020508 2216 18-19 -0.5673027185345703 2216 20-21 -0.5763350081812746 2216 22-23 -0.5394421337606303 2216 24-25 -0.5422063503355048 2216 26-27 -0.5143319035911631 2216 28-29 -0.5793062670071976 2216 30-31 -0.6038314272179264 2216 32-33 -0.6112312097227957 2216 34-35 -0.6420342036626749 2216 36-37 -0.6710097646237685 2216 38-39 -0.6319252796671861 2216 40-41 -0.7027258515309924 2216 42-43 -0.7175961015696615 2216 44-45 -0.7154720973796742 2216 46-47 -0.6668966632169528 2216 48-49 -0.6681432632445166 2216 50-51 -0.6936624897423158 2216 52-53 -0.7196576160463479 2216 54-55 -0.6775513124688217 2216 56-57 -0.6425869468407015 2216 58-59 -0.6562962402688797 2216 60-61 -0.672774846518088 2216 62-63 -0.6939708530586799 2216 64-65 -0.7119805602096463 2216 66-67 -0.7133102679134851 2216 68-69 -0.6877395368944761 2216 70-71 -0.7192696204019953 2216 72-73 -0.6930414151895832 2216 74-75 -0.6758629224835957 2216 76-77 -0.5824600647231009 2216 78-79 -0.5668360282153202 2216 80-81 -0.6018974493411235 2216 82-83 -0.6204146365777703 2216 84-85 -0.7359417885692068 2216 86-87 -0.6449924215983813 2216 88-89 -0.6893038761359094 2216 90-91 -0.6228219912434554 2216 92-93 -0.6157695381133088 2216 94-95 -0.6875174184845214 2216 96-97 -0.6528893053431339 2216 98-99 -0.7211832503741675 2216 100 -0.7096724782670378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 694.0 3 48.0 4 69.0 5 118.0 6 467.0 7 1304.0 8 3162.0 9 5957.0 10 7886.0 11 9920.0 12 14200.0 13 20443.0 14 25366.0 15 32896.0 16 40844.0 17 56704.0 18 56204.0 19 28280.0 20 19254.0 21 17290.0 22 17907.0 23 19522.0 24 23138.0 25 28363.0 26 36167.0 27 46610.0 28 61664.0 29 80867.0 30 107095.0 31 138550.0 32 181968.0 33 237086.0 34 323169.0 35 466386.0 36 770754.0 37 1526935.0 38 2452078.0 39 1148146.0 40 4701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05938270478723 21.87926516402098 15.394516347113903 22.66683578407789 2 15.059636464936274 21.408195389737568 40.530280526775876 23.00188761855028 3 17.438193098235544 26.31036223205277 31.517613363201075 24.733831306510613 4 12.168325051808413 18.483747060112737 39.848833256034666 29.499094632044187 5 11.798575734816207 38.2008438342444 36.57699778400205 13.42358264693734 6 28.64477388102491 40.048635842499145 17.91450985110453 13.392080425371415 7 24.7054246692424 33.755859180960265 23.694255718645486 17.844460431151845 8 23.462933831506206 36.945727347204496 21.146395027989772 18.44494379329953 9 23.77812768808414 17.148123389645704 23.08466126457962 35.98908765769053 10-11 22.495329629320842 27.903118389778005 29.820616329173514 19.78093565172764 12-13 23.607619219261796 25.584570153660437 29.939715773871185 20.868094853206582 14-15 20.84200967223533 27.720004163644198 27.915643020928556 23.522343143191918 16-17 19.319490048441054 30.924038205679032 28.73706412161835 21.019407624261564 18-19 19.02435931550488 29.01914602359498 32.128849061907 19.827645598993136 20-21 20.34352685625393 28.30801905890658 31.816831606552597 19.531622478286895 22-23 19.92769287682353 28.203578487438925 31.996744469567208 19.871984166170343 24-25 19.817404981296054 28.350273307795646 31.893115159708707 19.939206551199593 26-27 19.580747488958107 28.2787949195553 32.02581883754449 20.114638753942106 28-29 19.610714244705456 28.22764799533512 32.238044874499074 19.923592885460344 30-31 20.066917849902126 28.712058542634665 31.6394086926307 19.581614914832507 32-33 19.573970334284716 28.394880215351264 32.09414578645698 19.937003663907046 34-35 19.850385885945105 28.128349574374717 31.980244656531802 20.041019883148373 36-37 20.095436815700833 28.593377209689407 31.354874284404854 19.956311690204902 38-39 20.02250439703792 28.637129172318453 31.450578192389315 19.889788238254305 40-41 19.968973611781614 28.378767311698695 31.485655896274338 20.166603180245353 42-43 19.965741046292834 28.39410639658561 31.302947300945107 20.337205256176446 44-45 20.284192430255217 28.517218466011634 30.965275257319703 20.233313846413452 46-47 20.33140161543404 28.76645051329146 30.537684225030493 20.36446364624401 48-49 20.267867350489478 28.70163071072009 30.28671108552794 20.743790853262496 50-51 20.40571941930643 28.66474950987318 30.044518292826 20.88501277799439 52-53 20.452785073585172 28.765882630165052 29.91990226918609 20.861430027063687 54-55 20.58993196884955 29.15905120833048 29.70589769716528 20.545119125654686 56-57 20.464423557439567 28.785945753806814 29.73525912694272 21.014371561810897 58-59 20.33027208965514 28.425339968538026 30.13733910186101 21.107048839945822 60-61 20.470258400551558 28.806277217826988 29.746585587101286 20.976878794520164 62-63 20.34596064108139 28.770949894985304 29.847481569384332 21.03560789454897 64-65 20.34250965900553 28.55464258808928 29.950243453368437 21.152604299536755 66-67 20.250094480775097 28.643544379504686 29.801688971784575 21.304672167935646 68-69 20.773302054413936 28.751429692574288 29.641077894593902 20.834190358417874 70-71 20.709749068047625 28.597414796313426 29.59188423870961 21.100951896929338 72-73 20.48403112648542 28.657142372168888 29.76834611964836 21.090480381697336 74-75 20.644713425545085 28.63763198447969 29.54464129813855 21.173013291836675 76-77 20.660586614532917 28.64719505674588 29.375564450865753 21.316653877855455 78-79 20.752483833428272 28.67380443752612 29.553655095496723 21.020056633548887 80-81 20.5162740611253 28.590419258236004 29.57838785312095 21.314918827517744 82-83 20.415632015313722 28.67064740785552 29.757720125267102 21.15600045156365 84-85 20.398439675399164 28.49446448962261 29.59996275669827 21.507133078279953 86-87 20.52656563985495 28.67030234717502 29.389752218611115 21.413379794358914 88-89 20.344841100158582 28.49904657349322 29.50745829398686 21.648654032361335 90-91 20.15213773835292 28.456496838082423 29.532581620595476 21.858783802969178 92-93 20.293740996926623 28.669679698864027 29.338298015606085 21.698281288603265 94-95 20.099115241734513 28.37442603852867 29.5561256453215 21.97033307441531 96-97 20.036435874302786 28.580768840256965 29.43095796220207 21.951837323238177 98-99 20.035118061500977 28.517795516328302 29.369767651281652 22.077318770889065 100 20.118117356267025 28.482086258195473 29.278459189927965 22.12133719560955 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 876.0 1 607.0 2 531.5 3 709.5 4 482.0 5 377.0 6 874.5 7 2313.5 8 3878.5 9 4650.5 10 4156.0 11 3344.0 12 3585.0 13 4260.0 14 5095.5 15 6102.0 16 7057.0 17 8117.0 18 9639.5 19 11448.5 20 13872.5 21 17576.0 22 22995.0 23 31176.5 24 42282.5 25 56539.5 26 75568.5 27 98127.0 28 125300.5 29 154757.5 30 181921.5 31 207540.0 32 232416.0 33 255442.0 34 275867.0 35 291698.5 36 302747.5 37 310127.0 38 314872.0 39 315364.5 40 313247.5 41 308982.5 42 300356.0 43 292239.0 44 282549.5 45 272539.5 46 262996.5 47 252251.0 48 242910.5 49 235565.5 50 227095.0 51 210797.5 52 189808.5 53 171563.0 54 158224.0 55 147638.0 56 131451.5 57 114004.0 58 100341.0 59 87958.0 60 72873.0 61 55957.0 62 43325.5 63 33560.0 64 25778.5 65 19571.5 66 14611.0 67 10778.5 68 7923.5 69 5792.5 70 4177.0 71 3179.5 72 2416.0 73 1707.5 74 1274.0 75 950.0 76 633.0 77 464.0 78 332.0 79 199.0 80 118.5 81 75.0 82 49.0 83 32.5 84 15.0 85 8.0 86 7.0 87 3.5 88 2.5 89 3.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.5 99 4.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12333672648701757 2 0.0 3 0.0 4 0.0 5 0.011519914849981503 6 1.747332696638581E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.2480947833132723E-5 76-77 0.0 78-79 0.0 80-81 1.747332696638581E-4 82-83 6.55249761239468E-4 84-85 4.1187127849337985E-4 86-87 6.053259699069371E-4 88-89 7.23894974321698E-4 90-91 8.050211352370606E-4 92-93 2.1841658707982264E-4 94-95 2.9330227407861896E-4 96-97 6.677307090726007E-4 98-99 0.001191930518064175 100 1.3729042616445996E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8012212.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.659549838883116 #Duplication Level Percentage of deduplicated Percentage of total 1 71.93011522053682 42.19388178695685 2 16.652712330243453 19.53681217776999 3 5.735277370733453 10.092863663050725 4 2.3761762325571407 5.575417125586205 5 1.2159666861252063 3.566402921359154 6 0.6366693948835587 2.24080440600382 7 0.3675703455069622 1.5093057701092796 8 0.2403594947763688 1.127950381046654 9 0.14961003619400953 0.7898451637067649 >10 0.6249302785779389 6.162214191194627 >50 0.03950689048430933 1.604766226642366 >100 0.027869595572445626 3.22533511944908 >500 0.0022570307582066287 0.871550261506885 >1k 8.939545365982381E-4 0.789169015086643 >5k 2.1284628390008612E-5 0.08402719424562347 >10k+ 6.385388517002582E-5 0.6296545962854438 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20188 0.2519653748552834 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19771 0.24676081960886706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10472 0.13070048570856588 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.4961895666265446E-5 0.0 3 0.0 0.0 0.0 2.4961895666265446E-5 0.0 4 0.0 0.0 0.0 3.744284349939817E-5 0.0 5 0.0 0.0 0.0 4.992379133253089E-5 0.0 6 0.0 0.0 0.0 4.992379133253089E-5 0.0 7 0.0 0.0 0.0 4.992379133253089E-5 0.0 8 0.0 0.0 0.0 4.992379133253089E-5 0.0 9 0.0 0.0 0.0 6.240473916566362E-5 0.0 10-11 0.0 0.0 0.0 8.112616091536271E-5 0.0 12-13 0.0 0.0 0.0 8.736663483192907E-5 0.0 14-15 6.2404739165663614E-6 0.0 0.0 9.984758266506178E-5 0.0 16-17 2.4961895666265446E-5 0.0 0.0 9.984758266506178E-5 0.0 18-19 2.4961895666265446E-5 0.0 0.0 1.1232853049819451E-4 0.0 20-21 2.4961895666265446E-5 0.0 0.0 1.6225232183072542E-4 0.0 22-23 3.1202369582831804E-5 0.0 0.0 3.0578322191175173E-4 0.0 24-25 3.744284349939817E-5 0.0 0.0 4.805164915756098E-4 0.0 26-27 3.744284349939817E-5 0.0 0.0 0.0010171972484003169 0.0 28-29 4.992379133253089E-5 0.0 0.0 0.0026147585710413054 0.0 30-31 6.240473916566361E-5 0.0 0.0 0.008175020830701934 0.0 32-33 8.736663483192907E-5 0.0 0.0 0.022372098990890404 0.0 34-35 9.984758266506178E-5 0.0 0.0 0.04652897352191879 0.0 36-37 1.1856900441476088E-4 0.0 0.0 0.08538840460037753 0.0 38-39 1.3729042616445996E-4 0.0 0.0 0.1355742958374042 0.0 40-41 1.3729042616445996E-4 0.0 0.0 0.18815652905839236 0.0 42-43 1.3729042616445996E-4 0.0 0.0 0.2432848756373396 0.0 44-45 1.3729042616445996E-4 0.0 0.0 0.3161798514567513 0.0 46-47 1.3729042616445996E-4 0.0 0.0 0.4049693143416575 0.0 48-49 1.3729042616445996E-4 0.0 0.0 0.5003175153128749 0.0 50-51 1.4353090008102632E-4 0.0 0.0 0.5962772827279158 0.0 52-53 1.4977137399759267E-4 0.0 0.0 0.6924679476778697 0.0 54-55 1.4977137399759267E-4 0.0 0.0 0.80256862898785 0.0 56-57 1.4977137399759267E-4 0.0 0.0 0.9393286148694019 0.0 58-59 1.6849279574729175E-4 0.0 0.0 1.0897427576804009 0.0 60-61 1.934546914135572E-4 0.0 0.0 1.245005748724572 0.0 62-63 2.0593563924668992E-4 0.0 0.0 1.4031580791921132 0.0 64-65 2.1217611316325628E-4 0.0 0.0 1.574702466684606 0.0 66-67 2.2465706099638902E-4 0.0 0.0 1.7761636861331178 0.0 68-69 2.2465706099638902E-4 0.0 0.0 2.005457419249516 0.0 70-71 2.2465706099638902E-4 1.2480947833132723E-5 0.0 2.2475878072122906 0.0 72-73 2.2465706099638902E-4 1.2480947833132723E-5 0.0 2.5024487619648608 0.0 74-75 2.2465706099638902E-4 1.2480947833132723E-5 0.0 2.7635439501600807 0.0 76-77 2.3089753491295538E-4 1.2480947833132723E-5 0.0 3.0484028630295854 0.0 78-79 2.3713800882952174E-4 1.2480947833132723E-5 0.0 3.3758654913274886 0.0 80-81 2.3713800882952174E-4 1.2480947833132723E-5 0.0 3.7267361372864323 0.0 82-83 2.433784827460881E-4 1.2480947833132723E-5 0.0 4.086462015732984 0.0 84-85 2.496189566626545E-4 1.2480947833132723E-5 0.0 4.447829138819592 0.0 86-87 2.496189566626545E-4 1.2480947833132723E-5 0.0 4.8413284621026005 0.0 88 2.496189566626545E-4 1.2480947833132723E-5 0.0 5.157789134885597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13930 0.0 59.5921 1 GTATCAA 26110 0.0 40.24603 1 ATCAACG 31925 0.0 32.641407 3 TCAACGC 32330 0.0 32.232502 4 GTGGTAT 5740 0.0 31.809807 1 TGGTATC 5535 0.0 31.166103 2 TATCAAC 33615 0.0 31.126202 2 CAACGCA 34235 0.0 30.46639 5 AACGCAG 35500 0.0 29.457075 6 CGCAGAG 38845 0.0 26.678501 8 ACGCAGA 39005 0.0 26.605215 7 GCAGAGT 43160 0.0 23.935043 9 GAGTACT 33935 0.0 21.80634 12-13 CAGAGTA 42815 0.0 21.312254 10-11 GTACATG 17785 0.0 21.141418 1 AGAGTAC 40595 0.0 20.874262 10-11 TACATGG 17575 0.0 20.245783 2 GTACTTT 37630 0.0 19.658865 14-15 AGTACTT 35550 0.0 19.460598 12-13 ACATGGG 17485 0.0 19.382227 3 >>END_MODULE