FastQCFastQC Report
Wed 25 May 2016
SRR2049458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6610052
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.041.8456041
GTATCAA109350.037.245791
ATCAACG119000.032.8613133
TCAACGC119100.032.8327264
CAACGCA129400.030.2565365
TATCAAC138450.028.8558922
AACGCAG136950.028.7255696
ACGCAGA153150.025.2882487
CGCAGAG157600.024.3035938
GTACATG184300.022.5071951
TACATGG182300.021.6571432
GCAGAGT183850.020.3992849
GTGGTAT38350.020.357331
ACATGGG187700.020.0074163
AGAGTAC162350.017.78921310-11
CATGGGG133600.017.554544
CAGAGTA179550.016.8311110-11
TGGTATC41100.016.5819172
GTATTAG27950.016.3217981
GAGTACA139500.015.8453461
Produced by FastQC (version 0.11.5)