FastQCFastQC Report
Wed 25 May 2016
SRR2049458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6610052
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182080.2754592550860417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178040.26934735157907985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100870.1526009175116928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114650.057.4257931
GTATCAA207050.040.3674281
ATCAACG253550.032.8509033
TCAACGC255650.032.599444
TATCAAC267300.031.196212
CAACGCA267950.031.1380775
GTGGTAT46600.030.7008931
AACGCAG275750.030.2874876
TGGTATC51150.026.9258352
ACGCAGA315450.026.4010527
CGCAGAG320900.025.8208568
GCAGAGT353950.023.1575539
GTACATG176050.021.8131051
GAGTACT257450.021.57807412-13
TACATGG173250.021.1897092
CAGAGTA350450.020.61945210-11
AGAGTAC331350.020.37542510-11
GTACTTT286750.019.4469914-15
ACATGGG184400.019.1946983
AGTACTT273700.018.9489712-13
Produced by FastQC (version 0.11.5)