##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049458_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6610052
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797807944627365	31.0	31.0	34.0	30.0	34.0
2	31.97522742635005	33.0	31.0	34.0	30.0	34.0
3	32.114526481788644	33.0	31.0	34.0	30.0	34.0
4	35.84707956911686	37.0	35.0	37.0	35.0	37.0
5	35.85475560555348	37.0	35.0	37.0	35.0	37.0
6	35.960982606490845	37.0	35.0	37.0	35.0	37.0
7	35.939920593665526	37.0	35.0	37.0	35.0	37.0
8	35.95967172421639	37.0	35.0	37.0	35.0	37.0
9	37.68464257164694	39.0	37.0	39.0	35.0	39.0
10-11	37.654890612055695	39.0	37.0	39.0	35.0	39.0
12-13	37.63063724763436	39.0	37.0	39.0	35.0	39.0
14-15	38.94837256953501	40.0	38.0	41.0	36.0	41.0
16-17	38.95323274310096	40.0	38.0	41.0	36.0	41.0
18-19	39.02770031158605	40.0	38.5	41.0	36.0	41.0
20-21	39.05185700505835	40.0	39.0	41.0	36.0	41.0
22-23	39.02481516030434	40.0	39.0	41.0	35.0	41.0
24-25	39.002854516121815	40.0	39.0	41.0	35.0	41.0
26-27	38.90988928680137	40.0	38.0	41.0	35.0	41.0
28-29	38.82901163258625	40.0	38.0	41.0	35.0	41.0
30-31	38.720062187105334	40.0	38.0	41.0	35.0	41.0
32-33	38.5910745482789	40.0	38.0	41.0	35.0	41.0
34-35	38.47391162732154	40.0	38.0	41.0	34.0	41.0
36-37	38.33588427140967	40.0	38.0	41.0	34.0	41.0
38-39	38.22663906426152	40.0	38.0	41.0	34.0	41.0
40-41	38.13062158966375	40.0	38.0	41.0	34.0	41.0
42-43	37.98057995610322	40.0	38.0	41.0	33.0	41.0
44-45	37.8027694789693	40.0	38.0	41.0	33.0	41.0
46-47	37.55868395589021	40.0	37.0	41.0	33.0	41.0
48-49	37.3152762640899	40.0	37.0	41.0	32.5	41.0
50-51	37.15400620146407	40.0	37.0	41.0	32.0	41.0
52-53	37.034802071148604	40.0	37.0	41.0	32.0	41.0
54-55	36.88691843876569	40.0	36.0	41.0	31.0	41.0
56-57	36.735310176077284	39.5	36.0	41.0	31.0	41.0
58-59	36.55887684393406	39.0	35.5	41.0	31.0	41.0
60-61	36.37297891151235	39.0	35.0	41.0	31.0	41.0
62-63	36.153221790085766	39.0	35.0	41.0	31.0	41.0
64-65	35.93204349980908	38.5	35.0	40.5	30.5	41.0
66-67	35.67179350480147	38.0	35.0	40.0	30.0	41.0
68-69	35.38303291713893	37.0	35.0	40.0	30.0	41.0
70-71	35.100514035290495	37.0	35.0	39.5	29.5	41.0
72-73	34.76914508388134	36.0	34.5	39.0	29.0	41.0
74-75	34.44673324808942	36.0	34.0	39.0	29.0	41.0
76-77	34.13013407458822	35.5	34.0	38.5	29.0	40.0
78-79	33.8235686345584	35.0	34.0	37.0	29.0	39.0
80-81	33.51854962714363	35.0	34.0	37.0	29.0	39.0
82-83	33.24507106751959	35.0	34.0	36.5	29.0	39.0
84-85	32.966455558897266	35.0	34.0	36.0	29.0	37.5
86-87	32.72152707724538	35.0	34.0	36.0	28.5	37.0
88-89	32.50648807301364	35.0	34.0	35.5	28.0	37.0
90-91	32.32082947305104	35.0	33.5	35.0	27.0	36.0
92-93	32.1588294615534	35.0	33.0	35.0	27.0	36.0
94-95	32.002885302566455	35.0	33.0	35.0	27.0	36.0
96-97	31.861049126391137	35.0	33.0	35.0	27.0	36.0
98-99	31.731076850832643	35.0	33.0	35.0	26.5	35.5
100	31.623558785921805	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2723860984618014
1101	2	0.32857726757226047
1101	3	0.4538216941559625
1101	4	0.2746381541201046
1101	5	0.23696713372069667
1101	6	0.1970388571837134
1101	7	0.23859020572470513
1101	8	0.27628335359495537
1101	9	0.3512156727261839
1101	10-11	0.37216408528014
1101	12-13	0.3127892365537406
1101	14-15	0.4312177867272453
1101	16-17	0.4025086088815826
1101	18-19	0.4241491938868549
1101	20-21	0.42407245992076525
1101	22-23	0.3870106803949085
1101	24-25	0.41076839337960536
1101	26-27	0.3975815380142791
1101	28-29	0.39899863643271516
1101	30-31	0.4004568594115412
1101	32-33	0.4016606102572098
1101	34-35	0.4452731879955394
1101	36-37	0.4593175405184553
1101	38-39	0.46748594012747446
1101	40-41	0.43648007919043863
1101	42-43	0.4200271818677166
1101	44-45	0.43902828703632935
1101	46-47	0.4837161139944257
1101	48-49	0.466610101625875
1101	50-51	0.4298922341552327
1101	52-53	0.4636789106332131
1101	54-55	0.46192296675239675
1101	56-57	0.461322996855948
1101	58-59	0.41723406838657695
1101	60-61	0.3887796641591166
1101	62-63	0.4028339503346743
1101	64-65	0.3522825150808515
1101	66-67	0.4386675050217761
1101	68-69	0.4426929880458701
1101	70-71	0.44100876642262676
1101	72-73	0.4588970255536964
1101	74-75	0.45731215376851253
1101	76-77	0.4446872708475311
1101	78-79	0.39804562353031514
1101	80-81	0.4124059310949306
1101	82-83	0.413027964758804
1101	84-85	0.3924723483529391
1101	86-87	0.34796930567346607
1101	88-89	0.3445926788039131
1101	90-91	0.3980429206334506
1101	92-93	0.40003454100233427
1101	94-95	0.4237603379178694
1101	96-97	0.3842102777963099
1101	98-99	0.4511187524580933
1101	100	0.39459838837123584
1102	1	0.14644968831576577
1102	2	0.208338103220715
1102	3	0.2917702481658395
1102	4	0.21289043168052046
1102	5	0.1905571039001046
1102	6	0.16462864122856757
1102	7	0.20238171197320298
1102	8	0.18999821849993737
1102	9	0.202155217667233
1102	10-11	0.2333609285317877
1102	12-13	0.24592709849072492
1102	14-15	0.29980450638789335
1102	16-17	0.30225234671349455
1102	18-19	0.29418087338306975
1102	20-21	0.2885258766715495
1102	22-23	0.26178025831282525
1102	24-25	0.24892551868077817
1102	26-27	0.2458853160575103
1102	28-29	0.3177219101079416
1102	30-31	0.2925565924754494
1102	32-33	0.3393566570170208
1102	34-35	0.3390917848300319
1102	36-37	0.3302661597633261
1102	38-39	0.256495204765109
1102	40-41	0.23091783710301428
1102	42-43	0.1665066288429955
1102	44-45	0.219651328033585
1102	46-47	0.25853695215853634
1102	48-49	0.30867202427671714
1102	50-51	0.2692043995817812
1102	52-53	0.20128179733996632
1102	54-55	0.22387125991999568
1102	56-57	0.252977256993546
1102	58-59	0.25045600337737994
1102	60-61	0.19539252400624463
1102	62-63	0.19624695498783495
1102	64-65	0.15504524117672247
1102	66-67	0.11634536704079323
1102	68-69	0.15793791368189858
1102	70-71	0.16236333185604934
1102	72-73	0.2301141189952034
1102	74-75	0.1690038478508029
1102	76-77	0.1457931235000558
1102	78-79	0.13890934403003996
1102	80-81	0.1418621838097991
1102	82-83	0.13938550678263795
1102	84-85	0.10715546452278346
1102	86-87	0.10293304849761142
1102	88-89	0.09315182056930382
1102	90-91	0.10606121601981044
1102	92-93	0.10762008086676644
1102	94-95	0.07913904068696098
1102	96-97	0.10458596442383694
1102	98-99	0.08746895652250686
1102	100	0.12559808817311335
1103	1	0.2064368549136617
1103	2	0.2692673985210128
1103	3	0.37082147099786056
1103	4	0.24228929189784765
1103	5	0.23412152992218438
1103	6	0.2171965310597983
1103	7	0.24079027128911434
1103	8	0.20858429862335726
1103	9	0.3013094135981902
1103	10-11	0.268242078738929
1103	12-13	0.27580512893550235
1103	14-15	0.36461429876640494
1103	16-17	0.33439460486521
1103	18-19	0.34826508560201574
1103	20-21	0.34147755444910644
1103	22-23	0.3510612499130161
1103	24-25	0.3859307096415776
1103	26-27	0.33551867209827435
1103	28-29	0.36504011565657635
1103	30-31	0.32916871196750463
1103	32-33	0.3495072140801341
1103	34-35	0.3964356063593897
1103	36-37	0.386739117242513
1103	38-39	0.3478213301195652
1103	40-41	0.3738838412227281
1103	42-43	0.39789635462015127
1103	44-45	0.37739600926111905
1103	46-47	0.40048126881222856
1103	48-49	0.4029881062034306
1103	50-51	0.3911790842126095
1103	52-53	0.42448867633714116
1103	54-55	0.4109762896291471
1103	56-57	0.3775787690582888
1103	58-59	0.3702182249681698
1103	60-61	0.2904548171358101
1103	62-63	0.3095767990688998
1103	64-65	0.31422433308893005
1103	66-67	0.3144670649256298
1103	68-69	0.3038221744278573
1103	70-71	0.2677975818820286
1103	72-73	0.2600849670171499
1103	74-75	0.295343664489053
1103	76-77	0.23912863557685426
1103	78-79	0.27572998807097093
1103	80-81	0.23935792216595075
1103	82-83	0.26651414047401545
1103	84-85	0.2881202459140084
1103	86-87	0.24333017808095292
1103	88-89	0.2706237222375165
1103	90-91	0.2391394628785406
1103	92-93	0.22712380265220133
1103	94-95	0.23256814869328224
1103	96-97	0.24518705267713514
1103	98-99	0.30571674513593905
1103	100	0.2528607570576895
1104	1	0.11316235855996482
1104	2	0.16571019567935608
1104	3	0.2482534602780504
1104	4	0.18733803008961303
1104	5	0.1406839317813109
1104	6	0.12886200158712313
1104	7	0.1575161305826427
1104	8	0.1428806168389869
1104	9	0.2064591900073367
1104	10-11	0.19642312431408726
1104	12-13	0.18852497511002753
1104	14-15	0.26003238733320444
1104	16-17	0.22586455117722437
1104	18-19	0.17829717338218387
1104	20-21	0.23544117540082254
1104	22-23	0.2172199981938121
1104	24-25	0.198586961533465
1104	26-27	0.18698373876046048
1104	28-29	0.17090553271172126
1104	30-31	0.20581189936378763
1104	32-33	0.21179260208567996
1104	34-35	0.2049481714521093
1104	36-37	0.2083420416635846
1104	38-39	0.21539374264407485
1104	40-41	0.1761225780066482
1104	42-43	0.20933793828867664
1104	44-45	0.21741714882544727
1104	46-47	0.23119616800522635
1104	48-49	0.23011879280052483
1104	50-51	0.2513736373233115
1104	52-53	0.27327834647388016
1104	54-55	0.20924064623895333
1104	56-57	0.22929501716404843
1104	58-59	0.2595544163321222
1104	60-61	0.21662876799545927
1104	62-63	0.2594552635521339
1104	64-65	0.224507347864062
1104	66-67	0.23211900883934788
1104	68-69	0.22947595211412164
1104	70-71	0.25365209294326263
1104	72-73	0.25995118043613985
1104	74-75	0.2814777897838425
1104	76-77	0.2699711701281373
1104	78-79	0.2557640484422663
1104	80-81	0.2524793447781164
1104	82-83	0.2286411224868914
1104	84-85	0.2525546959960323
1104	86-87	0.20723176048327474
1104	88-89	0.17466611659409637
1104	90-91	0.1688700089413615
1104	92-93	0.22552338451649945
1104	94-95	0.1817334254241345
1104	96-97	0.1772835799382797
1104	98-99	0.20692406473131086
1104	100	0.13732421830324526
1105	1	0.1507435465522029
1105	2	0.16946173861539293
1105	3	0.3013759584792126
1105	4	0.19727759448694115
1105	5	0.1710671470268892
1105	6	0.1624051160674469
1105	7	0.13577925550123382
1105	8	0.11842485609928133
1105	9	0.19959267280049886
1105	10-11	0.17057763887360977
1105	12-13	0.16420015803180377
1105	14-15	0.24110197507419429
1105	16-17	0.2512520693057567
1105	18-19	0.21367312032921149
1105	20-21	0.283974638860343
1105	22-23	0.2488858368529563
1105	24-25	0.1999179352906637
1105	26-27	0.22450181426721372
1105	28-29	0.26829664773784856
1105	30-31	0.2164524706907187
1105	32-33	0.1749743105451529
1105	34-35	0.18277036396438007
1105	36-37	0.15833729916317907
1105	38-39	0.21103485770267838
1105	40-41	0.2061453978536676
1105	42-43	0.19308797492346486
1105	44-45	0.19689883227586336
1105	46-47	0.16713880803636982
1105	48-49	0.1909857238235304
1105	50-51	0.1646063617536484
1105	52-53	0.19922826649207792
1105	54-55	0.19754011200093657
1105	56-57	0.17484428334491042
1105	58-59	0.19441100537084566
1105	60-61	0.169705508968228
1105	62-63	0.17986152310721337
1105	64-65	0.14668950715329032
1105	66-67	0.14480292575419895
1105	68-69	0.19545185588295766
1105	70-71	0.2211216787101975
1105	72-73	0.20543581895565666
1105	74-75	0.20504536093400816
1105	76-77	0.17292375650949765
1105	78-79	0.1594088537332894
1105	80-81	0.14460680600271303
1105	82-83	0.11307606839013573
1105	84-85	0.09030702526504086
1105	86-87	0.13606221918891492
1105	88-89	0.12024301850018304
1105	90-91	0.10417195145307545
1105	92-93	0.11899726346926798
1105	94-95	0.10560854480375781
1105	96-97	0.11655260997335049
1105	98-99	0.15871318221020658
1105	100	0.1706823514576783
1106	1	0.06759462317152654
1106	2	0.13299349969680563
1106	3	0.23503245033705156
1106	4	0.17119454662442024
1106	5	0.11861289328219016
1106	6	0.1393688763825125
1106	7	0.12822365276338132
1106	8	0.15836116883464513
1106	9	0.1893855158140738
1106	10-11	0.17142641548579007
1106	12-13	0.16338204558805103
1106	14-15	0.20832273602732698
1106	16-17	0.21776770683832325
1106	18-19	0.23635056874110205
1106	20-21	0.23554101326396193
1106	22-23	0.2110025093824177
1106	24-25	0.21348216422234856
1106	26-27	0.18648695102461943
1106	28-29	0.22512254472733417
1106	30-31	0.24045632706869213
1106	32-33	0.2307335549876086
1106	34-35	0.21181999269873586
1106	36-37	0.2140738122728223
1106	38-39	0.18353975458585836
1106	40-41	0.18052831602879138
1106	42-43	0.2676965357473762
1106	44-45	0.2385653232706133
1106	46-47	0.20384830188687175
1106	48-49	0.2509262981055258
1106	50-51	0.17868771273585082
1106	52-53	0.160569271716831
1106	54-55	0.2138283105626826
1106	56-57	0.17525528203381668
1106	58-59	0.1846965410862751
1106	60-61	0.19155210193203231
1106	62-63	0.17338451264883759
1106	64-65	0.15242696599993621
1106	66-67	0.15277892672376225
1106	68-69	0.16493068876970085
1106	70-71	0.19672574364026474
1106	72-73	0.2230863834660184
1106	74-75	0.1816135781842405
1106	76-77	0.13909553269157726
1106	78-79	0.1229426965926379
1106	80-81	0.08356230588601221
1106	82-83	0.11865093302015595
1106	84-85	0.10360943382218579
1106	86-87	0.08286169011031319
1106	88-89	0.07685449236227271
1106	90-91	0.12514010772613204
1106	92-93	0.14368974149775937
1106	94-95	0.0819979236610493
1106	96-97	0.08711196972107516
1106	98-99	0.15222281893375822
1106	100	0.13467879060805998
1107	1	0.04139764471798202
1107	2	0.043032840955543605
1107	3	0.17537558232449868
1107	4	0.07963084078012628
1107	5	0.08543621956451375
1107	6	0.09308543159096416
1107	7	0.05924607047077757
1107	8	0.06756529621105756
1107	9	0.09266252859757884
1107	10-11	0.08424150933823427
1107	12-13	0.06286896462104608
1107	14-15	0.10173895971238522
1107	16-17	0.11199524217339984
1107	18-19	0.14306530372837756
1107	20-21	0.1307733450919102
1107	22-23	0.1274132992628978
1107	24-25	0.14486115272288913
1107	26-27	0.1230687551137919
1107	28-29	0.16836573815970013
1107	30-31	0.1273344559510221
1107	32-33	0.09802984219042798
1107	34-35	0.1633561500665337
1107	36-37	0.13924958572887647
1107	38-39	0.1508489864221758
1107	40-41	0.16278016556861274
1107	42-43	0.11955769652984571
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2216	74-75	-0.6190026605231012
2216	76-77	-0.4919833958358666
2216	78-79	-0.47027230603418246
2216	80-81	-0.4976653522847485
2216	82-83	-0.5249175644272484
2216	84-85	-0.6474873285885749
2216	86-87	-0.5622652846924083
2216	88-89	-0.5842752368432826
2216	90-91	-0.5459066283261471
2216	92-93	-0.5517358386857651
2216	94-95	-0.5649239678568669
2216	96-97	-0.5623366696079906
2216	98-99	-0.6447479128889952
2216	100	-0.6441473690059603
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	595.0
3	41.0
4	58.0
5	147.0
6	443.0
7	1046.0
8	2351.0
9	4241.0
10	5560.0
11	7290.0
12	10280.0
13	14548.0
14	17865.0
15	23341.0
16	29577.0
17	40429.0
18	40403.0
19	21281.0
20	15283.0
21	14264.0
22	15038.0
23	16442.0
24	19121.0
25	23998.0
26	30811.0
27	39698.0
28	52421.0
29	68991.0
30	89943.0
31	117506.0
32	153222.0
33	202142.0
34	278991.0
35	415609.0
36	705176.0
37	1358658.0
38	1958219.0
39	812461.0
40	2562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04412702971431	20.80122781091082	14.957219902606433	23.19742525676844
2	15.39402413173149	20.71215173496366	39.705497021808604	24.18832711149625
3	17.821024706008366	25.414081462596666	30.947532636657023	25.817361194737952
4	12.765436641043065	17.682916866614665	38.28594691842061	31.26569957392166
5	12.761075786571189	37.51198331051663	35.60441276331509	14.122528139597096
6	30.177794714978234	38.33562857949855	17.483225136795713	14.003351568727506
7	26.08515031349224	32.939861895186304	22.65222724420322	18.32276054711824
8	24.556644940160837	36.06411870889972	20.50492189773999	18.87431445319946
9	24.509126403241606	16.841425755803435	21.99363938438003	36.655808456574924
10-11	23.521244613506823	27.10808477754789	28.826876097192578	20.543794511752704
12-13	24.42283358739084	24.65619029925937	29.126586296144115	21.794389817205673
14-15	21.628929696770918	26.607687806389418	27.077290768665662	24.686091728174002
16-17	20.586547579353383	29.79899401699109	27.70198328243106	21.91247512122446
18-19	20.355808093491547	28.055913932295844	30.82763191575497	20.760646058457635
20-21	21.417289909368336	27.323937844967027	30.653072018192894	20.605700227471736
22-23	21.15177006171812	27.158243233184855	30.729115292890285	20.960871412206743
24-25	20.994010334563175	27.292546261360727	30.678729910142916	21.03471349393318
26-27	20.793837930473163	27.248030726535887	30.77498482614055	21.183146516850396
28-29	20.77104688435129	27.266858112462657	30.89102022192866	21.071074781257394
30-31	21.119387563063043	27.569662084352743	30.462975177804957	20.84797517477926
32-33	20.807324965068354	27.417151937685208	30.786512723349226	20.98901037389721
34-35	20.995152534352226	27.17692689860836	30.722209144496897	21.105711422542516
36-37	21.289567767394267	27.486364706359346	30.202576318612923	21.021491207633463
38-39	21.146596123600844	27.56259708698207	30.280003848683794	21.010802940733296
40-41	21.11753432499472	27.376289929337922	30.266183987660007	21.239991758007353
42-43	21.116081991488116	27.429701006890717	30.08449857883115	21.36971842279002
44-45	21.28480986231273	27.57292227050559	29.849356707027418	21.29291116015426
46-47	21.385497421200316	27.753185602775893	29.454556484578337	21.406760491445453
48-49	21.428590879466604	27.745439824074005	29.173605593420444	21.652363703038947
50-51	21.49157071684156	27.780681604320208	28.97526373468772	21.752483944150516
52-53	21.48231965497397	27.904674577446592	28.91711744476443	21.69588832281501
54-55	21.666471005069248	28.129589600807982	28.73814003278643	21.46579936133634
56-57	21.485375606727448	27.866278510365728	28.85860807146449	21.78973781144233
58-59	21.404665197792696	27.558867918134382	29.176442182300534	21.86002470177239
60-61	21.529860884604236	27.924039024201324	28.843630882177628	21.70246920901681
62-63	21.41182853024454	27.86708031948917	28.958887161553342	21.762203988712947
64-65	21.444286671269758	27.671469150318334	29.030407022516613	21.853837155895295
66-67	21.365807712254004	27.765719543507373	28.91466663197203	21.95380611226659
68-69	21.7186869331739	27.837905057327838	28.811369411314768	21.632038598183495
70-71	21.701674964130387	27.718390112513486	28.768434802025762	21.81150012133036
72-73	21.57880149808201	27.798344097746885	28.893622924600294	21.729231479570814
74-75	21.66080703002087	27.82662132057519	28.668233522840175	21.844338126563763
76-77	21.736931872850622	27.833971654080784	28.504337030934096	21.924759442134494
78-79	21.78748366881229	27.926255345646293	28.623995696251708	21.662265289289707
80-81	21.624979330687964	27.809260184619127	28.689179083760603	21.87658140093231
82-83	21.586315420575417	27.843265580783203	28.832076565094194	21.738342433547185
84-85	21.655255213822155	27.794444378887466	28.621037194104833	21.929263213185546
86-87	21.721066938415884	27.952973184936315	28.504953694514505	21.821006182133296
88-89	21.499316377994745	27.8312938137409	28.608826382703512	22.060563425560844
90-91	21.440398196340382	27.771141794057506	28.669620622857273	22.11883938674484
92-93	21.53210232623579	27.96794570679278	28.542572013612638	21.957379953358792
94-95	21.34095407246485	27.73666392714408	28.719246085401245	22.203135914989826
96-97	21.358832454829273	27.87204942567748	28.617200091588423	22.15191802790482
98-99	21.34583231876846	27.899229293780394	28.598921645182852	22.156016742268296
100	21.364396364078665	27.776032958328265	28.55064202295779	22.30892865463528
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1495.0
1	1036.5
2	1113.0
3	1565.5
4	988.5
5	660.5
6	1603.0
7	4135.0
8	6761.0
9	7745.5
10	6145.0
11	3857.0
12	3420.5
13	3851.0
14	4344.5
15	4934.5
16	5678.0
17	6599.0
18	7686.0
19	8967.0
20	10662.5
21	13013.0
22	16490.5
23	21564.5
24	28795.5
25	38466.0
26	50657.5
27	65157.5
28	82928.0
29	103767.0
30	123211.5
31	141418.5
32	159263.5
33	174870.0
34	188859.0
35	199418.5
36	208091.5
37	216725.0
38	225139.0
39	230163.0
40	231894.0
41	233299.5
42	230151.0
43	226358.0
44	224143.5
45	220970.0
46	217386.0
47	210996.0
48	204772.0
49	202090.0
50	197639.5
51	189122.0
52	177809.0
53	168037.0
54	161271.0
55	152711.0
56	139799.5
57	124969.5
58	113542.0
59	105462.0
60	93632.5
61	78677.0
62	65886.0
63	54377.0
64	44753.5
65	36584.5
66	29349.5
67	23158.5
68	18078.0
69	13901.5
70	10287.5
71	7669.5
72	5853.5
73	4484.0
74	3452.5
75	2483.0
76	1625.5
77	1105.5
78	738.0
79	459.0
80	264.0
81	125.5
82	70.5
83	44.0
84	19.5
85	9.5
86	7.5
87	4.5
88	4.5
89	5.5
90	2.5
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12683712624348492
2	0.0
3	0.0
4	0.0
5	0.013010487663334569
6	1.966701623527319E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	7.564237013566611E-6
76-77	0.0
78-79	0.0
80-81	1.6641321429846541E-4
82-83	6.429601461531619E-4
84-85	3.933403247054638E-4
86-87	5.446250649767959E-4
88-89	5.975747240717622E-4
90-91	7.034740422616947E-4
92-93	2.5718405846126473E-4
94-95	2.950052435290978E-4
96-97	7.034740422616947E-4
98-99	0.0011421997890485583
100	1.2102779221706577E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6610052.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.64664273399587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.783991562017	41.95752758329763
2	16.09249796040363	18.553569612418908
3	5.226723636775516	9.039092104755893
4	2.165845383905619	4.994148602523253
5	1.0931265761299143	3.150753859859865
6	0.6560644010743488	2.269194608353558
7	0.41818040389763966	1.6874687439291727
8	0.2740955944119723	1.264055264482336
9	0.2095080316490612	1.0869691185338517
>10	1.0031383585008233	10.157054445428699
>50	0.05388965134124323	2.123704812032774
>100	0.021362490617895417	2.16542227447924
>500	0.001076918835377906	0.42603565321670983
>1k	3.9397140080683093E-4	0.3473288588732091
>5k	2.6264759805404935E-5	0.07970091984381013
>10k+	7.87942794162148E-5	0.6979735379710871
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18208	0.2754592550860417	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17804	0.26934735157907985	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10087	0.1526009175116928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.5128474027133221E-5	0.0	0.0	0.0
4	0.0	1.5128474027133221E-5	0.0	0.0	0.0
5	0.0	1.5128474027133221E-5	0.0	0.0	0.0
6	0.0	1.5128474027133221E-5	0.0	0.0	0.0
7	0.0	1.5128474027133221E-5	0.0	0.0	0.0
8	0.0	1.5128474027133221E-5	0.0	0.0	0.0
9	0.0	1.5128474027133221E-5	0.0	0.0	0.0
10-11	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.0	0.0
12-13	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.0	0.0
14-15	1.5128474027133221E-5	1.5128474027133221E-5	0.0	1.5128474027133221E-5	0.0
16-17	1.5128474027133221E-5	1.5128474027133221E-5	0.0	1.5128474027133221E-5	0.0
18-19	1.5128474027133221E-5	1.5128474027133221E-5	0.0	3.0256948054266443E-5	0.0
20-21	1.5128474027133221E-5	1.5128474027133221E-5	0.0	6.0513896108532885E-5	0.0
22-23	1.5128474027133221E-5	1.5128474027133221E-5	0.0	1.0589931818993254E-4	0.0
24-25	1.5128474027133221E-5	1.5128474027133221E-5	0.0	2.5718405846126473E-4	0.0
26-27	1.5128474027133221E-5	1.5128474027133221E-5	0.0	5.219323539360962E-4	0.0
28-29	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.0010968143669671585	0.0
30-31	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.0026928683768297134	0.0
32-33	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.00658088620180295	0.0
34-35	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.013729090179623399	0.0
36-37	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.023691190326490624	0.0
38-39	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.03592256157742783	0.0
40-41	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.04767738589651034	0.0
42-43	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.06014324849486812	0.0
44-45	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.07571801250580176	0.0
46-47	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.09417475081890431	0.0
48-49	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.11383420281716392	0.0
50-51	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.13360711837062705	0.0
52-53	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.15386414509295843	0.0
54-55	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.17695019645836374	0.0
56-57	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.20329643397661623	0.0
58-59	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.23244900342690195	0.0
60-61	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.2627135157181819	0.0
62-63	1.5128474027133221E-5	1.5128474027133221E-5	0.0	0.29390842916213067	0.0
64-65	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.3294300861778394	0.0
66-67	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.36930117947634905	0.0
68-69	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.41480762934996573	0.0
70-71	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.4658057152954319	0.0
72-73	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.519126022004063	0.0
74-75	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.5766217875441827	0.0
76-77	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.6391553349353378	0.0
78-79	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.7110987931713699	0.0
80-81	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.7913099624632303	0.0
82-83	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.8757117190606065	0.0
84-85	1.5128474027133221E-5	4.5385422081399664E-5	0.0	0.9649545873466654	0.0
86-87	1.5128474027133221E-5	4.5385422081399664E-5	0.0	1.0623365746593219	0.0
88	1.5128474027133221E-5	4.5385422081399664E-5	0.0	1.1436823795032172	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11465	0.0	57.425793	1
GTATCAA	20705	0.0	40.367428	1
ATCAACG	25355	0.0	32.850903	3
TCAACGC	25565	0.0	32.59944	4
TATCAAC	26730	0.0	31.19621	2
CAACGCA	26795	0.0	31.138077	5
GTGGTAT	4660	0.0	30.700893	1
AACGCAG	27575	0.0	30.287487	6
TGGTATC	5115	0.0	26.925835	2
ACGCAGA	31545	0.0	26.401052	7
CGCAGAG	32090	0.0	25.820856	8
GCAGAGT	35395	0.0	23.157553	9
GTACATG	17605	0.0	21.813105	1
GAGTACT	25745	0.0	21.578074	12-13
TACATGG	17325	0.0	21.189709	2
CAGAGTA	35045	0.0	20.619452	10-11
AGAGTAC	33135	0.0	20.375425	10-11
GTACTTT	28675	0.0	19.44699	14-15
ACATGGG	18440	0.0	19.194698	3
AGTACTT	27370	0.0	18.94897	12-13
>>END_MODULE