FastQCFastQC Report
Wed 25 May 2016
SRR2049457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4699486
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49800.043.1607321
GTATCAA81850.034.994561
ATCAACG88700.031.1569613
TCAACGC88750.031.0318414
CAACGCA100000.027.6359335
TATCAAC100850.027.543112
AACGCAG102600.026.9819876
GTGGTAT29450.024.7541681
ACGCAGA118200.023.1028027
GTACATG161450.023.0430741
TACATGG160150.022.4803562
CGCAGAG122150.022.0840268
ACATGGG166950.020.663843
TGGTATC32450.019.6981562
GCAGAGT139900.018.543849
CATGGGT41850.017.0697214
CATGGGG114000.016.8615234
CAGAGTA131650.016.7789410-11
AGAGTAC121950.016.49489210-11
GAGTACA123050.016.244611
Produced by FastQC (version 0.11.5)