FastQCFastQC Report
Wed 25 May 2016
SRR2049457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4699486
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.13065258626156137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.11862999485475645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.053.8300971
GTATCAA130250.037.475341
GTGGTAT33450.031.239311
ATCAACG159350.030.177333
TCAACGC161650.029.7479574
TATCAAC166650.028.940052
CAACGCA171400.028.0557635
AACGCAG177350.027.189686
TGGTATC38100.026.0324292
ACGCAGA203450.023.4474857
GTACATG163600.023.2473051
CGCAGAG205750.022.979798
TACATGG164850.022.0703492
ACATGGG171300.020.6905123
GCAGAGT230800.020.2005799
GAGTACT137100.019.3172112-13
CAGAGTA224950.018.97089410-11
AGAGTAC216000.018.4296710-11
GTACCTG38600.017.9256361
GTACTTT150850.017.3539314-15
Produced by FastQC (version 0.11.5)