##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049457_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4699486 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.834611274509594 31.0 31.0 34.0 30.0 34.0 2 31.920538544002472 33.0 31.0 34.0 30.0 34.0 3 32.00751167255313 33.0 31.0 34.0 30.0 34.0 4 35.758946191136644 37.0 35.0 37.0 33.0 37.0 5 35.853722087904934 37.0 35.0 37.0 35.0 37.0 6 35.94791238871655 37.0 35.0 37.0 35.0 37.0 7 35.909327317923704 37.0 35.0 37.0 35.0 37.0 8 35.919486939635526 37.0 35.0 37.0 35.0 37.0 9 37.63979784172141 39.0 37.0 39.0 35.0 39.0 10-11 37.61984417019223 39.0 37.0 39.0 35.0 39.0 12-13 37.619427741672176 39.0 37.0 39.0 35.0 39.0 14-15 38.9408831093443 40.0 38.0 41.0 36.0 41.0 16-17 38.94450925058612 40.0 38.0 41.0 35.5 41.0 18-19 39.004887236604176 40.0 38.5 41.0 36.0 41.0 20-21 39.02367407839921 40.0 39.0 41.0 35.5 41.0 22-23 39.00139802523084 40.0 39.0 41.0 35.0 41.0 24-25 38.98980431051396 40.0 38.5 41.0 35.0 41.0 26-27 38.90413398401442 40.0 38.0 41.0 35.0 41.0 28-29 38.8269300089414 40.0 38.0 41.0 35.0 41.0 30-31 38.73804528835707 40.0 38.0 41.0 35.0 41.0 32-33 38.630030177768376 40.0 38.0 41.0 35.0 41.0 34-35 38.53031161280191 40.0 38.0 41.0 34.5 41.0 36-37 38.408213472707445 40.0 38.0 41.0 34.0 41.0 38-39 38.304710451313184 40.0 38.0 41.0 34.0 41.0 40-41 38.22174265440944 40.0 38.0 41.0 34.0 41.0 42-43 38.09295356981593 40.0 38.0 41.0 33.5 41.0 44-45 37.94309515976853 40.0 38.0 41.0 33.0 41.0 46-47 37.74036543570935 40.0 37.5 41.0 33.0 41.0 48-49 37.53250642729864 40.0 37.0 41.0 33.0 41.0 50-51 37.380256159928976 40.0 37.0 41.0 33.0 41.0 52-53 37.25602863802552 40.0 37.0 41.0 32.0 41.0 54-55 37.10562208292566 40.0 36.0 41.0 32.0 41.0 56-57 36.950118800226235 39.5 36.0 41.0 32.0 41.0 58-59 36.76442923758045 39.0 35.5 41.0 31.0 41.0 60-61 36.5635276921774 39.0 35.0 41.0 31.0 41.0 62-63 36.33154764159315 39.0 35.0 41.0 31.0 41.0 64-65 36.10320745715595 38.0 35.0 40.5 31.0 41.0 66-67 35.83483066020412 37.5 35.0 40.0 31.0 41.0 68-69 35.53522917187114 37.0 35.0 40.0 30.0 41.0 70-71 35.24165940700749 36.5 35.0 39.5 30.0 41.0 72-73 34.90512962481429 36.0 34.0 39.0 30.0 41.0 74-75 34.58147837869929 36.0 34.0 39.0 29.5 40.5 76-77 34.26367681486869 35.0 34.0 37.5 29.0 40.0 78-79 33.964390467383026 35.0 34.0 37.0 29.0 39.0 80-81 33.66638926044253 35.0 34.0 37.0 29.0 39.0 82-83 33.40621336035473 35.0 34.0 36.0 29.0 38.5 84-85 33.141932756050345 35.0 34.0 36.0 29.0 37.0 86-87 32.915704717494634 35.0 34.0 36.0 29.0 37.0 88-89 32.7167157216768 35.0 34.0 35.0 29.0 36.5 90-91 32.54305247424931 35.0 34.0 35.0 29.0 36.0 92-93 32.39698064852199 35.0 33.5 35.0 29.0 36.0 94-95 32.25175838378921 35.0 33.0 35.0 29.0 36.0 96-97 32.122406152502634 35.0 33.0 35.0 28.5 35.5 98-99 31.999241193611386 35.0 33.0 35.0 27.5 35.0 100 31.89919408207621 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3038162891296068 1101 2 0.33371400478092994 1101 3 0.4486625751910083 1101 4 0.2593271808701658 1101 5 0.28733754626848196 1101 6 0.23611815778811973 1101 7 0.2411105335973076 1101 8 0.2752494299206987 1101 9 0.31298291954527 1101 10-11 0.27140559527590824 1101 12-13 0.31353484294830736 1101 14-15 0.45174255068288716 1101 16-17 0.4403504052847538 1101 18-19 0.416095757400619 1101 20-21 0.4557107878006832 1101 22-23 0.4263189750443388 1101 24-25 0.44215141262650803 1101 26-27 0.43779072558174903 1101 28-29 0.4839010694012913 1101 30-31 0.47806769960970286 1101 32-33 0.4824833356918603 1101 34-35 0.44215966161743836 1101 36-37 0.5229790021190794 1101 38-39 0.507442475921323 1101 40-41 0.5002065383720264 1101 42-43 0.48103142434253243 1101 44-45 0.5155816160621569 1101 46-47 0.5102806144250636 1101 48-49 0.47319659069501085 1101 50-51 0.43120501312533577 1101 52-53 0.4840194590588709 1101 54-55 0.5008800809637108 1101 56-57 0.5419967626395206 1101 58-59 0.46683213921994593 1101 60-61 0.4219293077672006 1101 62-63 0.4964045185587551 1101 64-65 0.46314205878255166 1101 66-67 0.4605521172451219 1101 68-69 0.45311296395308887 1101 70-71 0.46667471446713904 1101 72-73 0.4803568723401028 1101 74-75 0.41179583396055364 1101 76-77 0.3930755331453284 1101 78-79 0.3774957700323469 1101 80-81 0.3662119879566603 1101 82-83 0.34868340797082453 1101 84-85 0.3199865498262753 1101 86-87 0.28618121474616487 1101 88-89 0.3249123184305134 1101 90-91 0.35006043809187304 1101 92-93 0.36230733328448395 1101 94-95 0.3212549195846677 1101 96-97 0.309274026238171 1101 98-99 0.38935652203876714 1101 100 0.42431957727914593 1102 1 0.21470448949108345 1102 2 0.24923720737229615 1102 3 0.3499519210117299 1102 4 0.22849836599797158 1102 5 0.23916042259174475 1102 6 0.2448702409747483 1102 7 0.22937544451907144 1102 8 0.18726513304019932 1102 9 0.2487605643120645 1102 10-11 0.23812759842122944 1102 12-13 0.2724218344747271 1102 14-15 0.3318217704561448 1102 16-17 0.359373244051973 1102 18-19 0.31245123706167277 1102 20-21 0.3428771258519987 1102 22-23 0.30805840653977157 1102 24-25 0.29000930995768925 1102 26-27 0.24426039907891095 1102 28-29 0.2977990343333303 1102 30-31 0.3462957586665709 1102 32-33 0.36769470578316543 1102 34-35 0.32599771292837687 1102 36-37 0.3355466616735754 1102 38-39 0.29715839968256574 1102 40-41 0.28105553961606944 1102 42-43 0.2228070035270946 1102 44-45 0.2888568548951085 1102 46-47 0.20765691421616594 1102 48-49 0.24203229475340038 1102 50-51 0.20816725756821342 1102 52-53 0.24383179621823103 1102 54-55 0.2285010818321922 1102 56-57 0.17240107688968465 1102 58-59 0.24336043534932372 1102 60-61 0.17066185007642787 1102 62-63 0.23985650550802262 1102 64-65 0.15434373472086804 1102 66-67 0.11308879306607622 1102 68-69 0.1693190996712417 1102 70-71 0.15146798019959817 1102 72-73 0.18586833866876162 1102 74-75 0.1408705470089302 1102 76-77 0.17776786701852387 1102 78-79 0.2168219911904785 1102 80-81 0.2082952332680179 1102 82-83 0.16337668074504563 1102 84-85 0.15463727864819532 1102 86-87 0.1982872284071746 1102 88-89 0.17000045776342887 1102 90-91 0.23100702636030235 1102 92-93 0.20759481303942806 1102 94-95 0.18800233173873693 1102 96-97 0.19101081707449907 1102 98-99 0.24264472944200577 1102 100 0.2208153639608348 1103 1 0.16174097791255093 1103 2 0.19318833919115264 1103 3 0.38410699568080275 1103 4 0.21398199805248197 1103 5 0.19937323057081358 1103 6 0.2019541971114407 1103 7 0.1881000779067179 1103 8 0.19620609801508238 1103 9 0.23988540328092967 1103 10-11 0.2578261054886397 1103 12-13 0.20697962164879158 1103 14-15 0.3309612245437492 1103 16-17 0.34365108392306354 1103 18-19 0.3511041751224795 1103 20-21 0.33544524146225285 1103 22-23 0.3566655938027168 1103 24-25 0.3304715983728741 1103 26-27 0.3348809693199115 1103 28-29 0.3643566486934944 1103 30-31 0.3893721154801497 1103 32-33 0.38420153081021624 1103 34-35 0.3493877269779233 1103 36-37 0.3038560928240628 1103 38-39 0.31410665201779864 1103 40-41 0.3463423433667998 1103 42-43 0.35932610507433793 1103 44-45 0.35906892224315357 1103 46-47 0.3567123964567287 1103 48-49 0.2815983410854841 1103 50-51 0.2983291078889323 1103 52-53 0.3751470895754423 1103 54-55 0.3491849423601465 1103 56-57 0.33511539979583205 1103 58-59 0.3127376544369582 1103 60-61 0.2791764023537553 1103 62-63 0.3178427746578265 1103 64-65 0.275825333406587 1103 66-67 0.2626010089891935 1103 68-69 0.26839070746896 1103 70-71 0.19330691090795682 1103 72-73 0.27445092800457616 1103 74-75 0.276231099654332 1103 76-77 0.25213441428649475 1103 78-79 0.2818120712235128 1103 80-81 0.265080434562563 1103 82-83 0.2539355317345695 1103 84-85 0.2216431767642959 1103 86-87 0.25174371970139475 1103 88-89 0.26119372654964224 1103 90-91 0.2486034430527866 1103 92-93 0.23908944009804856 1103 94-95 0.22809093814048254 1103 96-97 0.24184646207434213 1103 98-99 0.2578223350043771 1103 100 0.19276784687020765 1104 1 0.22722842334660953 1104 2 0.21263713352679048 1104 3 0.3052972806129617 1104 4 0.216894072409346 1104 5 0.20820887051748116 1104 6 0.1868742233573215 1104 7 0.19019644829047166 1104 8 0.2092633132759758 1104 9 0.3024761799989619 1104 10-11 0.29903715086472715 1104 12-13 0.24618459260432246 1104 14-15 0.34910860749493366 1104 16-17 0.3370779675269375 1104 18-19 0.3126828613990398 1104 20-21 0.3707656059532667 1104 22-23 0.30369743388898485 1104 24-25 0.2803880319029872 1104 26-27 0.30661442234025316 1104 28-29 0.31590687514352567 1104 30-31 0.3303285144225683 1104 32-33 0.3313603438874466 1104 34-35 0.349907898333349 1104 36-37 0.37401542238776386 1104 38-39 0.354565829067802 1104 40-41 0.35774172259468173 1104 42-43 0.3529891118162496 1104 44-45 0.24011737128617483 1104 46-47 0.2984268195356705 1104 48-49 0.3223223361596368 1104 50-51 0.3237281537361554 1104 52-53 0.30310428613040585 1104 54-55 0.30069660099245965 1104 56-57 0.2983805847621426 1104 58-59 0.2962901238795297 1104 60-61 0.26387999760919456 1104 62-63 0.22529799613228363 1104 64-65 0.1873952683337592 1104 66-67 0.18001708380637638 1104 68-69 0.1968603081001703 1104 70-71 0.1574283014275366 1104 72-73 0.2322539968168158 1104 74-75 0.19068909805463363 1104 76-77 0.2020814334926655 1104 78-79 0.16931203523404292 1104 80-81 0.16053888387329351 1104 82-83 0.17735530510879016 1104 84-85 0.23226011273316516 1104 86-87 0.2135588084372415 1104 88-89 0.1901613354699876 1104 90-91 0.2258396212385918 1104 92-93 0.2134241903635825 1104 94-95 0.1920490653938245 1104 96-97 0.15517418706684794 1104 98-99 0.21652682718180927 1104 100 0.22593206623158224 1105 1 0.19049421401686573 1105 2 0.17911177518367083 1105 3 0.30874335262844 1105 4 0.1884640508707207 1105 5 0.18687084104566054 1105 6 0.11859442880674465 1105 7 0.15758533871409952 1105 8 0.14365255602613303 1105 9 0.22387737439170508 1105 10-11 0.2065498607933094 1105 12-13 0.18239542655797436 1105 14-15 0.27913284228394275 1105 16-17 0.3132743367634703 1105 18-19 0.285254527327794 1105 20-21 0.2553602715786383 1105 22-23 0.21339445215463115 1105 24-25 0.30849762461700436 1105 26-27 0.2460472770089126 1105 28-29 0.22411496747083248 1105 30-31 0.26549238607881165 1105 32-33 0.25335583523612115 1105 34-35 0.2113317438293265 1105 36-37 0.23926162081603763 1105 38-39 0.24591774752184392 1105 40-41 0.27312345930480575 1105 42-43 0.21627192150874208 1105 44-45 0.19143859162182508 1105 46-47 0.22220654983457422 1105 48-49 0.2345665665534753 1105 50-51 0.22954742325134703 1105 52-53 0.1639499494404717 1105 54-55 0.21605264117742706 1105 56-57 0.2190951696618484 1105 58-59 0.22145902201668122 1105 60-61 0.21210674656779105 1105 62-63 0.18507322806675575 1105 64-65 0.1652027827360456 1105 66-67 0.16889125578303776 1105 68-69 0.18005202226289896 1105 70-71 0.2454521527804232 1105 72-73 0.27294657022654434 1105 74-75 0.22520179197214674 1105 76-77 0.18836411401812114 1105 78-79 0.11303125280866055 1105 80-81 0.182715233961261 1105 82-83 0.1679993226632277 1105 84-85 0.19437198631970176 1105 86-87 0.14461596472661853 1105 88-89 0.15835038540702584 1105 90-91 0.1063662303533448 1105 92-93 0.14880825573057166 1105 94-95 0.1362616189199528 1105 96-97 0.17341714370945027 1105 98-99 0.16318430448901466 1105 100 0.16893132482772089 1106 1 0.17144075736166542 1106 2 0.18835889832243424 1106 3 0.27837537678846047 1106 4 0.12155705674289408 1106 5 0.18506523824261478 1106 6 0.1322431803002857 1106 7 0.1411700269118512 1106 8 0.13195936657190543 1106 9 0.15338519663986006 1106 10-11 0.18572721193788766 1106 12-13 0.18283465340898886 1106 14-15 0.261002459969788 1106 16-17 0.2447858919759085 1106 18-19 0.2241335511734519 1106 20-21 0.2183543301751385 1106 22-23 0.2504097130586871 1106 24-25 0.28213761900754974 1106 26-27 0.2519647564220975 1106 28-29 0.22138152971606218 1106 30-31 0.2945045964803725 1106 32-33 0.3009930675042156 1106 34-35 0.29538036564060377 1106 36-37 0.27361501450208436 1106 38-39 0.27545659039328996 1106 40-41 0.2596109298616369 1106 42-43 0.25844931832290996 1106 44-45 0.3049140407491322 1106 46-47 0.2646607277465023 1106 48-49 0.27134577378754443 1106 50-51 0.24132154308706077 1106 52-53 0.2178819193456647 1106 54-55 0.28743337884293396 1106 56-57 0.27636971359534357 1106 58-59 0.22817565868881218 1106 60-61 0.20333286040545318 1106 62-63 0.20191281920937598 1106 64-65 0.24310271276314666 1106 66-67 0.19878694273431563 1106 68-69 0.19994003817592443 1106 70-71 0.2193920858155778 1106 72-73 0.2479906623571111 1106 74-75 0.24118580455049 1106 76-77 0.2390324028964912 1106 78-79 0.21517293858769904 1106 80-81 0.18201274130550615 1106 82-83 0.2114544391416544 1106 84-85 0.2224266281249001 1106 86-87 0.22935715453071026 1106 88-89 0.1974338492402694 1106 90-91 0.2551133263808225 1106 92-93 0.25122014650956714 1106 94-95 0.22822088293923315 1106 96-97 0.21928070557576262 1106 98-99 0.22607810381781945 1106 100 0.21569411763755042 1107 1 0.07491299658032702 1107 2 0.04493779119214736 1107 3 0.17564833468979657 1107 4 0.12591287809138407 1107 5 0.10663609676466734 1107 6 0.11213879810568983 1107 7 0.09480496912291159 1107 8 0.0896296034321935 1107 9 0.1146585348430662 1107 10-11 0.1204760832697147 1107 12-13 0.07862492037964586 1107 14-15 0.21889850106498443 1107 16-17 0.19147777560604595 1107 18-19 0.20509302188236234 1107 20-21 0.18457784867656812 1107 22-23 0.20698668010107468 1107 24-25 0.17242243200383456 1107 26-27 0.194532153318562 1107 28-29 0.23674676855629428 1107 30-31 0.2072548912770813 1107 32-33 0.17884566491577658 1107 34-35 0.2028958224641073 1107 36-37 0.196102680578214 1107 38-39 0.24299312306315812 1107 40-41 0.2316004381426211 1107 42-43 0.22356179919015062 1107 44-45 0.2609155148895468 1107 46-47 0.32751304432458284 1107 48-49 0.33757232811453974 1107 50-51 0.28347237294213556 1107 52-53 0.23370808201194393 1107 54-55 0.22083844630257943 1107 56-57 0.20641970352299666 1107 58-59 0.24706769139602613 1107 60-61 0.21873037940642348 1107 62-63 0.22001636094311294 1107 64-65 0.18035568912731748 1107 66-67 0.26530452840107444 1107 68-69 0.23019760954056778 1107 70-71 0.25826461628522424 1107 72-73 0.19191643430401228 1107 74-75 0.2233059098112662 1107 76-77 0.23664957399656572 1107 78-79 0.2064425082880561 1107 80-81 0.22945800780235714 1107 82-83 0.2408377594217228 1107 84-85 0.21231728976857056 1107 86-87 0.19228291926066987 1107 88-89 0.16945555923687294 1107 90-91 0.12919092961301715 1107 92-93 0.12407590839667648 1107 94-95 0.1966317451387951 1107 96-97 0.16744465082484794 1107 98-99 0.20120394545534737 1107 100 0.1781158921787913 1108 1 0.047216978606076765 1108 2 0.09427258104578584 1108 3 0.1688083388960564 1108 4 0.07202923359043467 1108 5 0.10282832671200737 1108 6 0.123469814324082 1108 7 0.1167740420716612 1108 8 0.0610973018338683 1108 9 0.08647329591796193 1108 10-11 0.06908738534588821 1108 12-13 0.08714966759764309 1108 14-15 0.17363001514696208 1108 16-17 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2215 14-15 -0.667236481091841 2215 16-17 -0.6158422278127347 2215 18-19 -0.6395883312022121 2215 20-21 -0.6180904033813164 2215 22-23 -0.5923832343744095 2215 24-25 -0.5864934464068554 2215 26-27 -0.5297742224445017 2215 28-29 -0.5082770232163867 2215 30-31 -0.5637985350362413 2215 32-33 -0.5842768589489538 2215 34-35 -0.5191734703274093 2215 36-37 -0.5998031758877005 2215 38-39 -0.566697256241639 2215 40-41 -0.613763061033687 2215 42-43 -0.6212331929841497 2215 44-45 -0.567993475076257 2215 46-47 -0.5779891334853531 2215 48-49 -0.5253933909418009 2215 50-51 -0.5108789101228979 2215 52-53 -0.5455298252259482 2215 54-55 -0.5652420555558848 2215 56-57 -0.5369683551973381 2215 58-59 -0.47475736952044656 2215 60-61 -0.5314432596687624 2215 62-63 -0.5571923732685278 2215 64-65 -0.5974199423939481 2215 66-67 -0.530852751630043 2215 68-69 -0.516130578281242 2215 70-71 -0.4433569878220496 2215 72-73 -0.4742581597860891 2215 74-75 -0.4177680239830224 2215 76-77 -0.4571777697752637 2215 78-79 -0.5019553036103517 2215 80-81 -0.5430773740151196 2215 82-83 -0.5436363992517812 2215 84-85 -0.5695629794426154 2215 86-87 -0.4952940928366374 2215 88-89 -0.4715717454038497 2215 90-91 -0.541992741584366 2215 92-93 -0.5200783794759367 2215 94-95 -0.4674394223731717 2215 96-97 -0.4327983711846315 2215 98-99 -0.44761305007146746 2215 100 -0.4406076446082352 2216 1 -0.38842117611061866 2216 2 -0.4290646611532125 2216 3 -0.3872360933168615 2216 4 -0.3483120706367444 2216 5 -0.3786830278874902 2216 6 -0.3655634892992339 2216 7 -0.42302072161511006 2216 8 -0.3657884055404992 2216 9 -0.5170618773570226 2216 10-11 -0.5280196568793798 2216 12-13 -0.5559485305170426 2216 14-15 -0.8194133442693854 2216 16-17 -0.7830355080053408 2216 18-19 -0.7457027468670674 2216 20-21 -0.7349806048092518 2216 22-23 -0.7650377305499774 2216 24-25 -0.7562229003240404 2216 26-27 -0.7478596028573747 2216 28-29 -0.8118785724049715 2216 30-31 -0.8052953924613249 2216 32-33 -0.7636919240938767 2216 34-35 -0.7854017449087749 2216 36-37 -0.8396697193894553 2216 38-39 -0.7513230602845198 2216 40-41 -0.7686145639757598 2216 42-43 -0.8208136277053555 2216 44-45 -0.9044977776927539 2216 46-47 -0.9401930712616178 2216 48-49 -0.8963545287532284 2216 50-51 -0.8358972694915252 2216 52-53 -0.88849272696892 2216 54-55 -0.9166182424005527 2216 56-57 -0.8597966189182316 2216 58-59 -0.8516117303976074 2216 60-61 -0.9048168990764438 2216 62-63 -0.9622315475464802 2216 64-65 -0.9713020199504427 2216 66-67 -0.8774763632898157 2216 68-69 -0.9289614695997486 2216 70-71 -0.9685610642383935 2216 72-73 -0.9427164710901721 2216 74-75 -0.9301416516550276 2216 76-77 -0.7784154517925899 2216 78-79 -0.7561032677890935 2216 80-81 -0.8002443155750427 2216 82-83 -0.7822843222562099 2216 84-85 -0.8588974294552045 2216 86-87 -0.7630347775953439 2216 88-89 -0.8025798985150168 2216 90-91 -0.8187741722068367 2216 92-93 -0.8431225845789285 2216 94-95 -0.7475524170268741 2216 96-97 -0.7862616681612451 2216 98-99 -0.8590894655429864 2216 100 -0.926924494689569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 231.0 3 28.0 4 28.0 5 76.0 6 195.0 7 485.0 8 1128.0 9 1970.0 10 2892.0 11 4389.0 12 6677.0 13 9327.0 14 10814.0 15 13662.0 16 16615.0 17 23030.0 18 22141.0 19 11510.0 20 8892.0 21 8567.0 22 9203.0 23 10483.0 24 12608.0 25 15959.0 26 21306.0 27 28400.0 28 37487.0 29 50064.0 30 65661.0 31 86681.0 32 112442.0 33 148696.0 34 205029.0 35 303511.0 36 529040.0 37 1009170.0 38 1394538.0 39 515507.0 40 1044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63151133485161 20.3462130879668 14.594273881374455 23.428001695807136 2 15.9613838619798 20.53156451577896 39.40622442539461 24.100827196846634 3 18.27172163083367 25.372774809840905 30.348574290890536 26.006929268434888 4 13.196251675183202 17.439843421174146 37.49716458353105 31.866740320111603 5 13.105428323713445 37.382936513847994 35.00951064345068 14.502124518987875 6 30.767759248308487 37.36584954806712 17.673228171366155 14.193163032258244 7 26.84855322475692 32.40473532637399 22.32418609184068 18.42252535702841 8 25.077593592150286 35.45494124251035 20.503072038090973 18.964393127248382 9 25.200032514194103 16.49176101386407 21.590893131716957 36.71731334022487 10-11 24.080261117918003 26.58397109811584 28.587796622864715 20.747971161101447 12-13 24.807542782338324 24.25032226928647 28.707288414094652 22.234846534280557 14-15 22.15453987946767 26.23333700749401 26.40810080081098 25.204022312227337 16-17 21.33447147198651 29.301491695049204 27.041510497105424 22.322526335858857 18-19 21.178518246463547 27.77800593511716 29.664425003074808 21.379050815344485 20-21 22.403216011282936 27.062023378726952 29.280766024199238 21.253994585790874 22-23 22.135963379824943 26.978397211950412 29.279521207212873 21.60611820101177 24-25 21.85961400885118 27.093409789921708 29.26816890187565 21.77880729935146 26-27 21.658549466899146 27.02984964738697 29.35033746243738 21.961263423276502 28-29 21.66141148202165 27.04899003848506 29.48742692285922 21.802171556634065 30-31 21.953443419131368 27.380739085082922 29.037218112789354 21.628599382996352 32-33 21.64270730884186 27.158342422979874 29.382053696936218 21.816896571242047 34-35 21.865103970944908 26.915560552792368 29.26187034071386 21.95746513554887 36-37 22.14734760354643 27.260215266095056 28.756495923171173 21.83594120718734 38-39 21.994001046071848 27.339043035770295 28.885584508603706 21.78137140955415 40-41 21.906023339573732 27.114997257146843 28.952272652796495 22.026706750482923 42-43 21.86058219984058 27.19846383200205 28.803543621579042 22.137410346578328 44-45 22.089330620412532 27.288388559940387 28.564613236426283 22.057667583220802 46-47 22.164955912199762 27.453225735750674 28.217468889150854 22.16434946289871 48-49 22.148603059994223 27.36018364561571 28.10620140160009 22.385011892789976 50-51 22.21108861692534 27.34081982582776 27.993848688984286 22.454242868262615 52-53 22.270244022431392 27.387335551164533 27.90247699429257 22.439943432111512 54-55 22.311014438600306 27.736320525265956 27.768228270070388 22.184436766063353 56-57 22.19239508320697 27.4252226732881 27.840831954813783 22.541550288691145 58-59 22.13217573155873 27.07380126252105 28.119500728377528 22.674522277542692 60-61 22.326877875580436 27.46556751100014 27.844396174390134 22.36315843902929 62-63 22.17160557558848 27.437617645844675 27.945173152978857 22.44560362558799 64-65 22.156540098214997 27.2643540165882 28.05292961826038 22.526176266936424 66-67 22.11009884910818 27.29279329696907 27.928713906159096 22.668393947763647 68-69 22.51282374285188 27.38999541652002 27.788730512230487 22.308450328397615 70-71 22.48977866941193 27.223604879342123 27.851205429700183 22.43541102154576 72-73 22.294906294007472 27.386122652562428 27.946949943036326 22.372021110393774 74-75 22.34812488004007 27.35372549253259 27.774101252775303 22.524048374652036 76-77 22.41195100911036 27.35122521909843 27.595166790580926 22.641656981210286 78-79 22.49042767655867 27.41155096536089 27.753811799843643 22.344209558236795 80-81 22.29141674943305 27.29435058934806 27.85133863428289 22.562894026936 82-83 22.225738001569546 27.3740341418822 27.886956085055097 22.513271771493162 84-85 22.263025886541648 27.34041867283304 27.74913506185486 22.647420378770452 86-87 22.392787773243928 27.412434694680076 27.684604031438482 22.51017350063752 88-89 22.225661150148674 27.321978558396687 27.69669166385429 22.75566862760035 90-91 22.113943570831392 27.250918139552105 27.77887365093227 22.856264638684237 92-93 22.17458684720326 27.504664353782722 27.625901377856916 22.6948474211571 94-95 22.017076179114223 27.238664441025858 27.817091308478265 22.927168071381658 96-97 22.106629720569966 27.324219660177906 27.75940805101183 22.809742568240303 98-99 22.020187318242797 27.37114915185026 27.718307869104702 22.89035566080224 100 22.10952185794094 27.194678051751236 27.789594682988465 22.906205407319362 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 91.0 1 88.5 2 171.5 3 250.5 4 168.5 5 125.5 6 268.0 7 600.5 8 963.5 9 1202.5 10 1146.5 11 990.0 12 1098.5 13 1290.5 14 1525.0 15 1859.0 16 2157.0 17 2525.0 18 2918.5 19 3322.0 20 3899.0 21 4747.0 22 6091.0 23 8238.0 24 11536.0 25 16129.0 26 22478.0 27 30701.0 28 41449.5 29 54643.0 30 68345.5 31 81614.5 32 93865.0 33 106112.0 34 119075.5 35 130347.0 36 140097.5 37 150217.0 38 159299.0 39 164678.5 40 169130.0 41 172544.0 42 172526.0 43 171599.5 44 169171.5 45 166521.0 46 165073.5 47 162387.0 48 159378.0 49 157246.0 50 154379.5 51 148342.0 52 138936.5 53 131106.5 54 125568.0 55 119674.5 56 110691.5 57 99339.0 58 90749.0 59 83818.5 60 74140.5 61 63361.0 62 53956.5 63 44661.5 64 35903.5 65 28999.5 66 23172.5 67 17786.0 68 13797.0 69 10635.5 70 7881.5 71 5945.5 72 4478.5 73 3197.5 74 2336.5 75 1713.0 76 1133.0 77 769.0 78 517.5 79 302.5 80 160.0 81 73.5 82 35.0 83 22.5 84 18.5 85 10.5 86 5.0 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1182256953207223 2 0.0 3 0.0 4 0.0 5 0.011490618335707352 6 1.0639461421951253E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.872654583926838E-4 82-83 8.085990680682952E-4 84-85 3.6174168834634255E-4 86-87 4.681363025658551E-4 88-89 5.106941482536601E-4 90-91 7.873201452243927E-4 92-93 2.9790491981463503E-4 94-95 3.298233040804888E-4 96-97 5.745309167853676E-4 98-99 0.0010745856036170765 100 8.511569137561002E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4699486.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.68316755390598 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43830381910749 45.77835230637366 2 15.034290552989493 18.246567453623346 3 4.475165772849987 8.147017032760829 4 1.7810204712342872 4.323118546913873 5 0.9103701353875315 2.7622071730896827 6 0.547440032225534 1.9932237120754661 7 0.3574300226301142 1.5182990166441739 8 0.2410634301303744 1.1702794017376654 9 0.1819407457696304 0.9936666684378986 >10 0.9619671044359321 10.255014632965153 >50 0.05032932834335771 2.06769430199582 >100 0.019695287618934246 2.056484843049279 >500 8.077088308707135E-4 0.3044215067122504 >1k 1.0535306782900595E-4 0.13400058890021044 >5k 7.023537855245828E-5 0.2496528147206525 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6140 0.13065258626156137 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5575 0.11862999485475645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1278922843902504E-5 0.0 10-11 0.0 1.0639461421951252E-5 0.0 2.1278922843902504E-5 0.0 12-13 0.0 2.1278922843902504E-5 0.0 3.191838426585376E-5 0.0 14-15 0.0 2.1278922843902504E-5 0.0 4.255784568780501E-5 0.0 16-17 0.0 2.1278922843902504E-5 0.0 4.255784568780501E-5 0.0 18-19 0.0 2.1278922843902504E-5 0.0 6.383676853170752E-5 0.0 20-21 0.0 2.1278922843902504E-5 0.0 7.447622995365876E-5 0.0 22-23 0.0 3.191838426585376E-5 0.0 9.575515279756127E-5 0.0 24-25 4.255784568780501E-5 4.255784568780501E-5 0.0 2.234286898609763E-4 1.0639461421951252E-5 26-27 4.255784568780501E-5 4.255784568780501E-5 0.0 3.936600726121963E-4 2.1278922843902504E-5 28-29 4.255784568780501E-5 4.255784568780501E-5 0.0 0.0010426672193512227 2.1278922843902504E-5 30-31 4.255784568780501E-5 4.255784568780501E-5 0.0 0.002681144278331715 2.1278922843902504E-5 32-33 4.255784568780501E-5 4.255784568780501E-5 0.0 0.006511350390234166 2.1278922843902504E-5 34-35 4.255784568780501E-5 4.255784568780501E-5 0.0 0.013533394928721992 2.1278922843902504E-5 36-37 4.255784568780501E-5 8.511569137561002E-5 0.0 0.023321699436917143 2.1278922843902504E-5 38-39 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.03554644061073913 2.1278922843902504E-5 40-41 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.048526583545519655 2.1278922843902504E-5 42-43 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.06235788339405629 2.1278922843902504E-5 44-45 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.07912567459505146 2.1278922843902504E-5 46-47 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.10040459743895397 2.1278922843902504E-5 48-49 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.12265171127225404 2.1278922843902504E-5 50-51 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.1443455731116126 2.1278922843902504E-5 52-53 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.1670501837860566 2.1278922843902504E-5 54-55 4.255784568780501E-5 1.0639461421951252E-4 0.0 0.1932445378069006 2.1278922843902504E-5 56-57 4.255784568780501E-5 1.595919213292688E-4 0.0 0.22348188716808604 2.1278922843902504E-5 58-59 4.255784568780501E-5 2.3406815128292755E-4 0.0 0.2586133887833691 2.1278922843902504E-5 60-61 4.255784568780501E-5 2.3406815128292755E-4 0.0 0.2936065774001667 2.1278922843902504E-5 62-63 4.255784568780501E-5 2.447076127048788E-4 0.0 0.33027016146021076 2.1278922843902504E-5 64-65 4.255784568780501E-5 2.5534707412683006E-4 0.0 0.37124272739614506 2.1278922843902504E-5 66-67 4.255784568780501E-5 2.5534707412683006E-4 0.0 0.4198118687873525 2.1278922843902504E-5 68-69 4.255784568780501E-5 2.5534707412683006E-4 0.0 0.47685002147043315 2.1278922843902504E-5 70-71 4.255784568780501E-5 2.5534707412683006E-4 0.0 0.5369416995816139 2.1278922843902504E-5 72-73 4.255784568780501E-5 2.5534707412683006E-4 0.0 0.601289162261575 2.1278922843902504E-5 74-75 4.255784568780501E-5 2.5534707412683006E-4 0.0 0.6689348579823411 2.1278922843902504E-5 76-77 4.255784568780501E-5 2.7662599697073255E-4 0.0 0.7451772385320437 2.1278922843902504E-5 78-79 4.255784568780501E-5 2.7662599697073255E-4 0.0 0.8331336661073148 2.1278922843902504E-5 80-81 4.255784568780501E-5 2.7662599697073255E-4 0.0 0.9286121929079052 2.1278922843902504E-5 82-83 4.255784568780501E-5 2.7662599697073255E-4 0.0 1.0332193776085299 2.1278922843902504E-5 84-85 4.255784568780501E-5 2.7662599697073255E-4 0.0 1.1412950267327107 2.1278922843902504E-5 86-87 4.255784568780501E-5 2.7662599697073255E-4 0.0 1.25933985120926 2.1278922843902504E-5 88 4.255784568780501E-5 2.7662599697073255E-4 0.0 1.3581272505120774 2.1278922843902504E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7520 0.0 53.830097 1 GTATCAA 13025 0.0 37.47534 1 GTGGTAT 3345 0.0 31.23931 1 ATCAACG 15935 0.0 30.17733 3 TCAACGC 16165 0.0 29.747957 4 TATCAAC 16665 0.0 28.94005 2 CAACGCA 17140 0.0 28.055763 5 AACGCAG 17735 0.0 27.18968 6 TGGTATC 3810 0.0 26.032429 2 ACGCAGA 20345 0.0 23.447485 7 GTACATG 16360 0.0 23.247305 1 CGCAGAG 20575 0.0 22.97979 8 TACATGG 16485 0.0 22.070349 2 ACATGGG 17130 0.0 20.690512 3 GCAGAGT 23080 0.0 20.200579 9 GAGTACT 13710 0.0 19.31721 12-13 CAGAGTA 22495 0.0 18.970894 10-11 AGAGTAC 21600 0.0 18.42967 10-11 GTACCTG 3860 0.0 17.925636 1 GTACTTT 15085 0.0 17.35393 14-15 >>END_MODULE